BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	961,712	G→C	8.8%	L236V (CTG→GTG)	selD ←	selenide, water dikinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	961,712	0	G	C	8.8%	871.1 / 55.4	271	L236V (CTG→GTG)	selD	selenide, water dikinase
Reads supporting (aligned to +/- strand): ref base G (107/140); new base C (12/12); total (119/152)
Fisher's exact test for biased strand distribution p-value = 5.28e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.00e-01

Only 100 of 273 total aligned reads displayed.

GCTGATCTTGTGCCCGTAGCTGTCGTAGTTGCGCAGGGTTCCACCGGGGATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTCGCGAGCCAGGCCTTGGTCCTGCACACGCAGCTTGGCCTTCTTTTCGGCA  >  NC_002947/961563‑961860

     tCTTGTGCCCGTAGCTGTCGTAGTTGCGCAGGGTTCCACCGGGGATGCAGCCTTCGGCCAGGTACTGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGc                                                                                                                                                 >  1:677336/1‑150 (MQ=255)

        tgtgCCCGTAGCTGTCGTAGTTGCGCAGGGTTCCACCGGGGATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGa                                                                                                                                              <  1:3794721/150‑1 (MQ=255)

        tgtgCCCGTAGCTGTCGTAGTTGCGCAGGGTTCCACCGGGGATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGa                                                                                                                                              <  2:1525978/150‑1 (MQ=255)

         gtgCCCGTAGCTGTCGTAGTTGCGCAGGGTTCCACCGGGGATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCACCTGGTCGAGCTGGCCGaa                                                                                                                                             <  1:2846819/150‑2 (MQ=255)

            cccGTAGCTGTCGTAGTTGCGCAGGGTTCCACCGGGGTGGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGGCCACCTGGTCGAGctg                                                                                                                                          <  2:602066/150‑3 (MQ=255)

             ccGTAGCTGTCGTAGTTGCGCAGGGTTCCACCGGGGATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCTGCTCGGCCACCTGGTCGAGCTgg                                                                                                                                         <  1:2492096/150‑1 (MQ=255)

              cGTAGCTGTCGTAGTTGCGCAGGGTTCCACCGGGGATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCTGCTCGGCCACCTGGTCGAGCTGGc                                                                                                                                        <  1:7449386/150‑1 (MQ=255)

              cGTAGCTGTCGTAGTTGCGCAGGGTTCCACCGGGGATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGACCAGCTCGACCAGGTGGCCGAGCAGGc                                                                                                                                        <  2:4207813/150‑1 (MQ=255)

                    tGTCGTAGTTGCGCAGGGTTCCACCGGGGATGCAGCCTTCGGCCAGGTAATGGTCGACACTCGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCTGCTCGGCCACCTGGTCGAGCTGGCCGAAgg                                                                                                                                  <  1:9036082/150‑2 (MQ=38)

                     gTCGTAGTTGCGCAGGGTTCCACCGGGGATGCAGCCTTTGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCACCTCGACCCGGTGGCCGAGCAGGCCGAAccc                                                                                                                                 >  1:3025496/1‑150 (MQ=255)

                      tCGTAGTTGCGCAGGGTTCCACCGGGGATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCg                                                                                                                                >  1:8379701/1‑150 (MQ=255)

                      tCGTAGTTGCGCAGGGTTCCACCGGGGATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCg                                                                                                                                >  2:4499738/1‑150 (MQ=255)

                       cGTAGTTGCGCAGGGTTCCACCGGGGATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCgg                                                                                                                               <  2:4786149/150‑1 (MQ=255)

                       cGTAGTTGCGCAGGGTTCCACCGGGGATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCgg                                                                                                                               <  1:7240687/150‑1 (MQ=255)

                        gTAGTTGCGCAGGGTTCCACCGGGGATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGaa                                                                                                                                    >  2:1385844/1‑144 (MQ=255)

                        gTAGTTGCGCAGGGTTCCACCGGGGATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGaa                                                                                                                                    <  1:1385844/144‑1 (MQ=255)

                        gTAGTTGCGCAGGGTTCCACCGGGGATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGt                                                                                                                              >  2:2316164/1‑150 (MQ=255)

                         tAGTTGCGCAGGGTTCCACCGGGGATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTg                                                                                                                             >  1:6926749/1‑150 (MQ=255)

                         tAGTTGCGCAGGGTTCCACCGGGGATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTg                                                                                                                             >  1:8674922/1‑150 (MQ=255)

                              gcgcAGGGTTCCACCGGGGATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTc                                                                                                                        <  1:1574906/150‑1 (MQ=255)

                               cgcAGGGTTCCACCGGGGATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCg                                                                                                                       <  1:5916297/150‑1 (MQ=255)

                                gcAGGGTTCCACCGGGGATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCgg                                                                                                                      >  1:209650/1‑150 (MQ=255)

                                  aGGGTTCCACCGGGGATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTc                                                                                                                    >  1:5220794/1‑150 (MQ=255)

                                     gTTCCACCGGGGATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATg                                                                                                                 <  1:805752/150‑1 (MQ=255)

                                     gTTCCACCGGGGATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATg                                                                                                                 <  1:8197120/150‑1 (MQ=255)

                                      ttCCACCGGGGATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATgg                                                                                                                >  1:5573418/1‑150 (MQ=255)

                                        ccACCGGGGATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGcc                                                                                                              <  1:8554033/150‑1 (MQ=255)

                                          aCCGGGGATGCAGCCTTCGGCCAGGTCATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCACGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCtt                                                                                                            >  1:945139/1‑150 (MQ=255)

                                          aCCGGGGATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCtt                                                                                                            <  1:2959823/150‑1 (MQ=255)

                                           ccGGGGATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTg                                                                                                           >  1:5702100/1‑150 (MQ=255)

                                            cGGGGATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGa                                                                                                          <  1:3975673/150‑1 (MQ=255)

                                            cGGGGATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGa                                                                                                          <  1:7666232/150‑1 (MQ=255)

                                             ggggATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGc                                                                                                                                        <  2:7888814/119‑1 (MQ=255)

                                             ggggATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGAc                                                                                                         >  1:8541260/1‑150 (MQ=255)

                                                gATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGAcccc                                                                                                      >  2:8700819/1‑150 (MQ=255)

                                                 aTGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGCGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCg                                                                                                     >  2:2570628/1‑150 (MQ=255)

                                                     aGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCa                                                                                                 <  1:3605051/150‑1 (MQ=255)

                                                     aGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCa                                                                                                 >  1:6595370/1‑150 (MQ=255)

                                                         ttCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGttt                                                                                             <  2:9021483/150‑1 (MQ=255)

                                                         ttCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGttt                                                                                             <  1:4995241/150‑1 (MQ=255)

                                                         ttCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGttt                                                                                             <  1:7266292/150‑1 (MQ=255)

                                                           cGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGc                                                                                           <  1:7130330/150‑1 (MQ=255)

                                                           cGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGc                                                                                           <  2:341406/150‑1 (MQ=255)

                                                            ggCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGcc                                                                                          >  2:7925448/1‑150 (MQ=255)

                                                             gCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCg                                                                                         >  1:4357390/1‑150 (MQ=255)

                                                                aGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAg                                                                                      >  2:2753831/1‑150 (MQ=255)

                                                                   tAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTc                                                                                                   >  2:8010641/1‑134 (MQ=255)

                                                                      tGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTg                                                                                >  1:4616461/1‑150 (MQ=255)

                                                                       ggTCGACACGAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGc                                                                               <  1:5390318/150‑1 (MQ=255)

                                                                        gTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGcc                                                                              >  1:6208038/1‑150 (MQ=255)

                                                                         tCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCg                                                                             <  2:7695687/150‑1 (MQ=255)

                                                                          cGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGGGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCgg                                                                            <  2:2584416/150‑1 (MQ=255)

                                                                              acTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGTCGTCGGTCAAGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCgt                                                                        >  1:1089079/1‑150 (MQ=255)

                                                                               cTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCgtg                                                                       <  2:4233335/150‑1 (MQ=255)

                                                                                 aggcaggcGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGtt                                                                     <  1:4899938/150‑1 (MQ=255)

                                                                                    caggcGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAg                                                                  >  1:1710312/1‑150 (MQ=255)

                                                                                      ggcGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAggg                                                                <  1:8562983/150‑1 (MQ=255)

                                                                                         gTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGt                                                               >  2:6566299/1‑148 (MQ=255)

                                                                                         gTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGc                                                             <  2:3758715/150‑1 (MQ=255)

                                                                                          tGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAgg                                                                 <  1:2135505/145‑1 (MQ=255)

                                                                                                ggcggcGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCa                                                      <  2:7197626/150‑1 (MQ=255)

                                                                                                      gTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTcgcg                                                <  2:3342931/150‑1 (MQ=255)

                                                                                                       tAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTCGCGa                                               <  1:1037315/150‑1 (MQ=255)

                                                                                                       tAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTCGCGa                                               <  1:4078483/150‑1 (MQ=255)

                                                                                                       tAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTCGCGa                                               <  2:3845084/150‑1 (MQ=255)

                                                                                                            cAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTCGCGAGCCAg                                          <  1:1002792/150‑1 (MQ=255)

                                                                                                              ggTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTCGCGAGCCAGGc                                        <  1:7671241/150‑1 (MQ=255)

                                                                                                               gTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCg                                                                         >  1:223088/1‑116 (MQ=255)

                                                                                                               gTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGGTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTCGCGCGCCAGGcc                                       >  1:3456121/1‑150 (MQ=255)

                                                                                                                  cgcgGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTCGCGAGCCAGGCCTTg                                    <  2:3680263/150‑1 (MQ=255)

                                                                                                                   gcgGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTCGCGAGCCAGGCCTTgg                                   <  1:3238626/150‑1 (MQ=255)

                                                                                                                     ggTGATGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTCGCGAGCCAGGCCTTGGTc                                 >  2:2969135/1‑150 (MQ=255)

                                                                                                                     ggTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGTCAGAGCAGGCCGAAGCCGGTGACGTCTGTCATGGCCTTGACCCCGTCCTGTTTGCAGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTCGCGAGACAGGCCTTGGTc                                 <  2:5703237/150‑1 (MQ=255)

                                                                                                                     ggTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTCGCGAGCCAGGCCTTGGTc                                 >  2:6094080/1‑150 (MQ=255)

                                                                                                                       tGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTCGCGAGCCAGGCCTTGGTCCt                               <  2:2997830/150‑1 (MQ=255)

                                                                                                                       tGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTCGCGAGCCAGGCCTTGGTCCt                               <  2:8964373/150‑1 (MQ=255)

                                                                                                                       tGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTCGCGAGCCAGGCCTTGGTCCt                               <  2:8528951/150‑1 (MQ=255)

                                                                                                                       tGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTCGCGAGCCAGGCCTTGGTCCt                               <  2:1008329/150‑1 (MQ=255)

                                                                                                                       tGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTCGCGAGCCAGGCCTTGGTCCt                               <  2:2517310/150‑1 (MQ=255)

                                                                                                                        gAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTCGCGAGCCAGGCCTTGGTCCTg                              <  2:3582196/150‑1 (MQ=255)

                                                                                                                           gCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGGGTTGAGGGTGCACATCCAGTCGCGAGCCAGGCCTTGGTCCTGcac                           >  2:709389/1‑150 (MQ=255)

                                                                                                                              accggcTTCGGCCTGCTCGGCCACCTGGTCGAGCTGGCCGAAGGCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTCGCGAGCCAGGCCTTGGTCCTGCACACg                        >  2:4643708/6‑150 (MQ=37)

                                                                                                                                   cTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTCGCGAGCCAGGCCTTGGTCCTGCACACGCAGCt                   >  1:3145528/1‑150 (MQ=255)

                                                                                                                                    ttCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTCGCGAGCCAGGCCTTGGTCCTGCACACGCAGCtt                  <  2:8085333/150‑1 (MQ=255)

                                                                                                                                    ttCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTCGCGAGCCAGGCCTTGGTCCTGCACACGCAGCtt                  >  1:1288679/1‑150 (MQ=255)

                                                                                                                                     tCGGCCAGCTCGACCAGGTGGCCGAGCCGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTCGCGAGCCAGGCCTTGGTCCTGCACACGCAGCTTg                 <  2:2013600/150‑1 (MQ=255)

                                                                                                                                     tCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTCGCGAGCCAGGCCTTGGTCCTGCACACGCAGCTTg                 <  2:6691531/150‑1 (MQ=255)

                                                                                                                                      cGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTCGCGAGCCAGGCCTTGGTCCTGCACACGCAGCTTgg                >  1:8770179/1‑150 (MQ=255)

                                                                                                                                       ggCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTCGCGAGCCAGGCCTTGGTCCTGCACACGCAGCTTGGc               >  2:1695676/1‑150 (MQ=255)

                                                                                                                                       ggCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTCGCGAGCCAGGCCTTGGTCCTGCACACGCAGCTTGGc               <  1:8841630/150‑1 (MQ=255)

                                                                                                                                        gCCTGCTCGGCCACCTGGTCGAGCTGGCCGAAGGCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTCGCGAGCCAGGCCTTGGTCCTGCACACGCAGCTTGGcc              >  2:7402436/1‑150 (MQ=255)

                                                                                                                                        gCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTCGCGAGCCAGGCCTTGGTCCTGCACACGCAGCTTGGcc              >  1:7914462/1‑150 (MQ=255)

                                                                                                                                         cctgCTCGGCCACCTGGTCGAGCTGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTCGCGAGCCAGGCCTTGGTCCTGCACACGCAGCTTGGCct             >  1:8317175/4‑150 (MQ=255)

                                                                                                                                          ctgCTCGGCCACCTGGTCGAGCTGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTCGCGAGCCAGGCCTTGGTCCTGCACACGCAGCTTGGCctt            >  2:6727858/3‑150 (MQ=255)

                                                                                                                                             cTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTCGCGAGCCAGGCCTTGGTCCTGCACACGCAGCTTGGCcttctt         <  2:5493931/150‑1 (MQ=255)

                                                                                                                                                 aCCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTCGCGAGCCAGGCCTTGGTCCTGCACACGCAGCTTGGCCTTCTTTTCg     <  2:6378503/150‑1 (MQ=255)

                                                                                                                                                  ccAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTCGCGAGCCAGGCCTTGGTCCTGCACACGCAGCTTGGCCTTCTTTTCgg    <  1:3294929/150‑1 (MQ=255)

                                                                                                                                                    aGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTCGCGAGCCAGGCCTTGGTCCTGCACACGCAGCTTGGCCTTCTTTTCGGCa  >  2:5201264/1‑150 (MQ=255)

                                                                                                                                                    aGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTCGCGAGCCAGGCCTTGGTCCTGCACACGCAGCTTGGCCTTCTTTTCGGCa  >  1:4956055/1‑150 (MQ=255)

                                                                                                                                                    aGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTCGCGAGCCAGGCCTTGGTCCTGCACACGCAGCTTGGCCTTCTTTTCGGCa  >  1:814807/1‑150 (MQ=255)

GCTGATCTTGTGCCCGTAGCTGTCGTAGTTGCGCAGGGTTCCACCGGGGATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTCGCGAGCCAGGCCTTGGTCCTGCACACGCAGCTTGGCCTTCTTTTCGGCA  >  NC_002947/961563‑961860

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`