Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 961,723 A→T 8.0% L232Q (CTG→CAG)  selD ← selenide, water dikinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947961,7230AT 8.0% 840.7 / 46.2 265L232Q (CTG→CAG) selDselenide, water dikinase
Reads supporting (aligned to +/- strand):  ref base A (111/131);  new base T (12/9);  total (123/142)
Fisher's exact test for biased strand distribution p-value = 3.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.27e-01

Only 100 of 270 total aligned reads displayed.

GCCCGTAGCTGTCGTAGTTGCGCAGGGTTCCACCGGGGATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTCGCGAGCCAGGCCTTGGTCCTGCACACGCAGCTTGGCCTTCTTTTCGGCAGTGGTGAGGATG  >  NC_002947/961574‑961872
                                                                                                                                                     |                                                                                                                                                     
gCCCGTAGCTGTCGTAGTTGCGCAGGGTTCCACCGGGGATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCa                                                                                                                                                       <  1:8478209/150‑1 (MQ=255)
 cccGTAGCTGTCGTAGTTGCGCAGGGATCCACCGGGGATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGAGGCCGAGCAg                                                                                                                                                      >  2:2625175/1‑150 (MQ=255)
  ccGTAGCTGTCGTAGTTGCGCAGGGTTCCACCGGGGATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCTGCTCGGCCACCTGGTCGAGCTgg                                                                                                                                                     <  1:2492096/150‑1 (MQ=255)
   cGTAGCTGTCGTAGTTGCGCAGGGTTCCACCGGGGATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCTGCTCGGCCACCTGGTCGAGCTGGc                                                                                                                                                    <  1:7449386/150‑1 (MQ=255)
      aGCTGTCGTAGTTGCGCAGGGTTCCACCGGGGATGCAGCCTTCGGCCAGGAAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGTCCAGCTCGACCAGGTGGCCGAGCAGGCCGa                                                                                                                                                 >  2:7868788/1‑150 (MQ=255)
            cGTAGTTGCGCAGGGTTCCACCGGGGATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCgg                                                                                                                                           <  1:7240687/150‑1 (MQ=255)
            cGTAGTTGCGCAGGGTTCCACCGGGGATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCgg                                                                                                                                           <  1:2174243/150‑1 (MQ=255)
             gTAGTTGCGCAGGGTTCCACCGGGGATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGaa                                                                                                                                                >  2:1385844/1‑144 (MQ=255)
              tAGTTGCGCAGGGTTCCACCGGGGATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTg                                                                                                                                         >  1:8674922/1‑150 (MQ=255)
              tAGTTGCGCAGGGTTCCACCGGGGATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTg                                                                                                                                         >  1:6926749/1‑150 (MQ=255)
                gTTGCGCAGGGTTCCACCGGGGATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGAc                                                                                                                                       >  2:806878/1‑150 (MQ=255)
                    cgcAGGGTTCCACCGGGGATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCg                                                                                                                                   <  1:5916297/150‑1 (MQ=255)
                       aGGGTTCCACCGGGGATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTc                                                                                                                                >  1:5220794/1‑150 (MQ=255)
                        gggTTCCACCGGGGATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCa                                                                                                                               >  1:8192642/1‑150 (MQ=255)
                         ggTTCCACCGGGGATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCAt                                                                                                                              >  2:3987307/1‑150 (MQ=255)
                          gTTCCACCGGGGATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCGGGCCGAAGCCGGTGACGTCGGTCATg                                                                                                                             <  1:1294049/150‑1 (MQ=255)
                          gTTCCACCGGGGATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATg                                                                                                                             <  1:806878/150‑1 (MQ=255)
                           ttCCACCGGGGATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATgg                                                                                                                            <  1:5515071/150‑1 (MQ=255)
                           ttCCACCGGGGATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATgg                                                                                                                            <  2:5246776/150‑1 (MQ=255)
                               aCCGGGGATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCtt                                                                                                                        <  1:2959823/150‑1 (MQ=255)
                                ccGGGGATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTg                                                                                                                       >  1:3315942/1‑150 (MQ=255)
                                ccGGGGATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTg                                                                                                                       >  1:5702100/1‑150 (MQ=255)
                                 cGGGGATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGa                                                                                                                      <  1:3975673/150‑1 (MQ=255)
                                 cGGGGATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGa                                                                                                                      <  1:7666238/150‑1 (MQ=255)
                                  ggggATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGc                                                                                                                                                    >  1:7888814/1‑119 (MQ=255)
                                  ggggATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGAc                                                                                                                     >  1:8541260/1‑150 (MQ=255)
                                     gATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGAcccc                                                                                                                  >  2:8700819/1‑150 (MQ=255)
                                      aTGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGCGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCg                                                                                                                 >  2:2570628/1‑150 (MQ=255)
                                       tGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGt                                                                                                                >  1:6179568/1‑150 (MQ=255)
                                          aGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCa                                                                                                             <  1:2544402/150‑1 (MQ=255)
                                          aGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTAGACCCCGACCa                                                                                                             >  2:6088223/1‑150 (MQ=255)
                                              ttCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGttt                                                                                                         <  2:9021483/150‑1 (MQ=255)
                                              ttCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGttt                                                                                                         <  1:7266292/150‑1 (MQ=255)
                                                cGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGc                                                                                                       <  2:341303/150‑1 (MQ=255)
                                                cGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGc                                                                                                       <  1:7130330/150‑1 (MQ=255)
                                                 ggCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGcc                                                                                                      <  2:8659584/150‑1 (MQ=255)
                                                    cAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGaa                                                                                                   <  1:3087758/150‑1 (MQ=255)
                                                    cAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGaa                                                                                                   >  2:4512500/1‑150 (MQ=255)
                                                     aGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCTGCTCGGCCACCTGGTCGAGCTGGCCGaa                                                                                                                                                <  1:1560929/104‑1 (MQ=37)
                                                      ggTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGc                                                                                                 >  1:5312604/1‑150 (MQ=255)
                                                             gTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGcc                                                                                          >  2:798371/1‑150 (MQ=255)
                                                             gTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGcc                                                                                          <  1:3681002/150‑1 (MQ=255)
                                                             gTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGcc                                                                                          >  2:3577319/1‑150 (MQ=255)
                                                                 acacTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGc                                                                                      <  2:333426/150‑1 (MQ=255)
                                                                   acTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGTCGTCGGTCAAGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCgt                                                                                    >  1:1089079/1‑150 (MQ=255)
                                                                    cTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCgtg                                                                                   <  2:4233061/150‑1 (MQ=255)
                                                                      aggcaggcGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGtt                                                                                 <  1:4899938/150‑1 (MQ=255)
                                                                        gcaggcGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGa                                                                               <  1:1287844/150‑1 (MQ=255)
                                                                        gcaggcGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGa                                                                               >  2:2343951/1‑150 (MQ=255)
                                                                        gcaggcGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGa                                                                               <  2:1244440/150‑1 (MQ=255)
                                                                         caggcGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAg                                                                              <  2:2788946/150‑1 (MQ=255)
                                                                          aggcGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAgg                                                                             <  1:178683/150‑1 (MQ=255)
                                                                           ggcGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAggg                                                                            <  1:8562925/150‑1 (MQ=255)
                                                                              gTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGt                                                                           >  2:6566299/1‑148 (MQ=255)
                                                                              gTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGt                                                                           <  1:6566299/148‑1 (MQ=255)
                                                                              gTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGc                                                                         <  2:3758715/150‑1 (MQ=255)
                                                                                     ggcggcGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCa                                                                  <  2:7197626/150‑1 (MQ=255)
                                                                                        ggcgTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTc                                                               <  1:3499727/150‑1 (MQ=255)
                                                                                          cgTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTcgc                                                             <  2:5685964/150‑1 (MQ=255)
                                                                                            tAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTCGCGa                                                           <  2:3845084/150‑1 (MQ=255)
                                                                                            tAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTCGCGa                                                           <  1:1037315/150‑1 (MQ=255)
                                                                                            tAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTCGCGa                                                           <  1:4078483/150‑1 (MQ=255)
                                                                                                 cAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTCGCGAGCCAg                                                      <  1:1002792/150‑1 (MQ=255)
                                                                                                   ggTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTCGCGAGCCAGGc                                                    >  2:2981441/1‑150 (MQ=255)
                                                                                                    gTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCg                                                                                     <  2:223088/116‑1 (MQ=255)
                                                                                                    gTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCg                                                                                     >  1:223088/1‑116 (MQ=255)
                                                                                                       cgcgGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTCGCGAGCCAGGCCTTg                                                <  1:9041507/150‑1 (MQ=255)
                                                                                                       cgcgGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTCGCGAGCCAGGCCTTg                                                <  2:7903851/150‑1 (MQ=255)
                                                                                                       cgcgGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTCGCGAGCCAGGCCTTg                                                <  1:4364413/150‑1 (MQ=255)
                                                                                                       cgcgGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTCGCGAGCCAGGCCTTg                                                <  2:3680263/150‑1 (MQ=255)
                                                                                                        gcgGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTCGCGAGCCAGGCCTTgg                                               <  1:8007510/150‑1 (MQ=255)
                                                                                                        gcgGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTCGCGAGCCAGGCCTTgg                                               <  1:6799567/150‑1 (MQ=255)
                                                                                                        gcgGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTCGCGAGCCAGGCCTTgg                                               <  1:3238699/150‑1 (MQ=255)
                                                                                                          ggTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTCGCGAGCCAGGCCTTGGTc                                             >  2:6094080/1‑150 (MQ=255)
                                                                                                           gTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTCGCGAGCCAGGCCTTGGTcc                                            <  1:1707011/150‑1 (MQ=255)
                                                                                                            tGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTCGCGAGCCAGGCCTTGGTCCt                                           <  2:2517310/150‑1 (MQ=255)
                                                                                                            tGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTCGCGAGCCAGGCCTTGGTCCt                                           <  2:8964373/150‑1 (MQ=255)
                                                                                                            tGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTCGCGAGCCAGGCCTTGGTCCt                                           <  2:1008329/150‑1 (MQ=255)
                                                                                                                gCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGGGTTGAGGGTGCACATCCAGTCGCGAGCCAGGCCTTGGTCCTGcac                                       >  2:709389/1‑150 (MQ=255)
                                                                                                                gCCGCTGCCTTCGGCCAGCACGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCAAGGCCATGACCCCGGCCAGTTTGCCGAAGCGGCTGCCCGGCGTGTTTAGGGTGCACAACCACTCGCGAGCCAGGCCTTGGTCCTGcac                                       >  1:4110891/1‑150 (MQ=255)
                                                                                                                   accggcTTCGGCCTGCTCGGCCACCTGGTCGAGCTGGCCGAAGGCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTCGCGAGCCAGGCCTTGGTCCTGCACACg                                    >  2:4643870/6‑150 (MQ=37)
                                                                                                                        cTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTCGCGAGCCAGGCCTTGGTCCTGCACACGCAGCt                               >  1:3145590/1‑150 (MQ=255)
                                                                                                                          tCGGCCAGCTCGACCAGGTGGCCGAGCCGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTCGCGAGCCAGGCCTTGGTCCTGCACACGCAGCTTg                             <  2:2013600/150‑1 (MQ=255)
                                                                                                                          tCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTCGCGAGCCAGGCCTTGGTCCTGCACACGCAGCTTg                             <  2:6691531/150‑1 (MQ=255)
                                                                                                                           cGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTCGCGAGCCAGGCCTTGGTCCTGCACACGCAGCTTgg                            >  1:8770294/1‑150 (MQ=255)
                                                                                                                            ggCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTCGCGAGCCAGGCCTTGGTCCTGCACACGCAGCTTGGc                           <  1:8841630/150‑1 (MQ=255)
                                                                                                                            ggCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTCGCGAGCCAGGCCTTGGTCCTGCACACGCAGCTTGGc                           >  2:1695676/1‑150 (MQ=255)
                                                                                                                             gCCTGCTCGGCCACCTGGTCGAGCTGGCCGAAGGCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTCGCGAGCCAGGCCTTGGTCCTGCACACGCAGCTTGGcc                          >  2:7402436/1‑150 (MQ=255)
                                                                                                                              cctgCTCGGCCACCTGGTCGAGCTGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTCGCGAGCCAGGCCTTGGTCCTGCACACGCAGCTTGGCct                         >  1:8317325/4‑150 (MQ=255)
                                                                                                                               ctgCTCGGCCACCTGGTCGAGCTGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTCGCGAGCCAGGCCTTGGTCCTGCACACGCAGCTTGGCctt                        >  1:4853579/3‑150 (MQ=255)
                                                                                                                                  cTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTCGCGAGCCAGGCCTTGGTCCTGCACACGCAGCTTGGCcttctt                     <  2:5493931/150‑1 (MQ=255)
                                                                                                                                      aCCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTCGCGAGCCAGGCCTTGGTCCTGCACACGCAGCTTGGCCTTCTTTTCg                 <  2:6378503/150‑1 (MQ=255)
                                                                                                                                      aCCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTCGCGAGCCAGGCCTTGGTCCTGCACACGCAGCTTGGCCTTCTTTTCg                 >  1:8262418/1‑150 (MQ=255)
                                                                                                                                         aGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTCGCGAGCCAGGCCTTGGTCCTGCACACGCAGCTTGGCCTTCTTTTCGGCa              >  1:4956055/1‑150 (MQ=255)
                                                                                                                                             ggCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTCGCGAGCCAGGCCTTGGTCCTGCACACGCAGCTTGGCCTTCTTTTCGGCAgtgg          <  2:4432308/150‑1 (MQ=255)
                                                                                                                                              gCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTCGCGAGCCAGGCCTTGGTCCTGCACACGCAGCTTGGCCTTCTTTTCGGCAgtggt         >  2:8328921/1‑150 (MQ=255)
                                                                                                                                                cGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTCGCGAGCCAGGCCTTGGTCCTGCTCACGCAGCTTGGCCTTCTTTTCGGCAGTGGTGa       <  2:8192642/150‑1 (MQ=255)
                                                                                                                                                cGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTCGCGAGCCAGGCCTTGGTCCTGCACACGCAGCTTGGCCTTCTTTTCGGCAGTGGTGa       >  2:505599/1‑150 (MQ=255)
                                                                                                                                                cGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCATTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTCGCGAGCCAGGCCTTGGTCCTGCACACGCAGCTTGGCCTTCTTTTCGGCAGTGGTGa       <  2:677336/150‑1 (MQ=255)
                                                                                                                                                 gAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTCGCGAGCCAGGCCTTGGTCCTGCACACGCAGCTTGGCCTTCTTTTCGGCAGTGGTGAg      <  2:7583060/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GCCCGTAGCTGTCGTAGTTGCGCAGGGTTCCACCGGGGATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTCGCGAGCCAGGCCTTGGTCCTGCACACGCAGCTTGGCCTTCTTTTCGGCAGTGGTGAGGATG  >  NC_002947/961574‑961872

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑