BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	14,826	C→G	5.8%	T499T (ACC→ACG)	gyrB →	DNA gyrase subunit B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	14,826	0	C	G	5.8%	872.6 / 31.0	259	T499T (ACC→ACG)	gyrB	DNA gyrase subunit B
Reads supporting (aligned to +/- strand): ref base C (121/123); new base G (8/7); total (129/130)
Fisher's exact test for biased strand distribution p-value = 7.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

Only 100 of 261 total aligned reads displayed.

GGTAAAATCCTCAACGTCGAGAAAGCGCGCTTCGACAAGATGATTTCGTCCCAGGAAGTGGGCACGCTGATCACTGCGCTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCA  >  NC_002947/14677‑14974

ggTAAAATCCTCAACGTCGAGAAAGCGCGCTTCGACAAGATGATTTCGTCCCAGGAAGTGGGCACGCTGATCACTGCGCTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACAGCGTTATCACAACATCATCATCATGAcc                                                                                                                                                      >  1:8171438/1‑150 (MQ=255)

  tAAAATCCTCAACGTCGAGAAAGCGCGCTTCGACAAGATGATTTCGTCCCAGGAAGTGGGCACGCTGATCACTGCGCTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGa                                                                                                                                                    >  1:863151/1‑150 (MQ=255)

   aaaaTCCTCAACGTCGAGAAAGCGCGCTTCGACAAGATGATTTCGTCCCAGGAAGTGGGCACGCTGATCACTGCGCTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGAt                                                                                                                                                   <  2:1053977/150‑1 (MQ=255)

    aaaTCCTCAACGTCGAGAAAGCGCGCTTCGACAAGATGATTTCGTCCCAGGAAGTGGGCACGCTGATCACTGCGCTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATg                                                                                                                                                  >  2:549321/1‑150 (MQ=255)

    aaaTCCTCAACGTCGAGAAAGCGCGCTTCGACAAGATGATTTCGTCCCAGGAAGTGGGCACGCTGATCACTGCGCTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATg                                                                                                                                                  <  1:6873439/150‑1 (MQ=255)

         cTCAACGTCGAGAAAGCGCGCTTCGACACGATGATTTCGTCCCAGGAAGTGGGCACGCTGATCACTGCGCTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGAc                                                                                                                                             <  1:8842463/150‑1 (MQ=255)

          tCAACGTCGAGAAAGCGCGCTTCGACAAGATGATTTCGTCCCAGGAAGTGGGCACGCTGATCACTGCGCTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACg                                                                                                                                            >  1:5637739/1‑150 (MQ=255)

            aaCGTCGAGAAAGCGCGCTTCGACAAGATGATTTCGTCCCAGGAAGTGGGCACGCTGATCACTGCGCTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGtt                                                                                                                                          <  1:8449149/150‑1 (MQ=255)

              cGTCGAGAAAGCGCGCTTCGACAAGATGATTTCGTCCCAGGAAGTGGGCACGCTGATCACTGCGCTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGa                                                                                                                                        <  2:1821660/150‑1 (MQ=255)

              cGTCGAGAAAGCGCGCTTCGACAAGATGATTTCGTCCCAGGAAGTGGGCACGCTGATCACTGCGCTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGa                                                                                                                                        <  1:2904823/150‑1 (MQ=255)

              cGTCGAGAAAGCGCGCTTCGACAAGATGATTTCGTCCCAGGAAGTGGGCACGCTGATCACTGCGCTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGa                                                                                                                                        <  2:3781546/150‑1 (MQ=255)

               gTCGAGAAAGCGCGCTTCGACAAGATGATTTCGTCCCAGGAAGTGGGCACGCTGATCACTGCGCTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGAc                                                                                                                                       >  2:8253571/1‑150 (MQ=255)

                tCGAGAAAGCGCGCTTCGACAAGATGATTTCGTCCCAGGAAGTGGGCACGCTGATCACTGCGCTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACg                                                                                                                                      >  1:2628063/1‑150 (MQ=255)

                tCGAGAAAGCGCGCTTCGACAAGATGATTTCGTCCCAGGAAGTGGGCACGCTGATCACTGCGCTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACg                                                                                                                                      <  2:3604491/150‑1 (MQ=255)

                  gagaAAGCGCGCTTCGACAAGATGATTTCGTCCCAGGAAGTGGGCACGCTGATCACTGCGCTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGt                                                                                                                                    >  1:7237379/1‑150 (MQ=255)

                  gagaAAGCGCGCTTCGACAAGATGATTTCGTCCCAGGAAGTGGGCACGCTGATCACTGCGCTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGt                                                                                                                                    >  1:1850596/1‑150 (MQ=255)

                  gagaAAGCGCGCTTCGACAAGATGATTTCGTCCCAGGAAGTGGGCACGCTGATCACTGCGCTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGt                                                                                                                                    <  1:6384914/150‑1 (MQ=255)

                  gagaAAGCGCGCTTCGACAAGATGATTTCGTCCCAGGAAGTGGGCACGCTGATCACTGCGCTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGt                                                                                                                                    >  2:4670773/1‑150 (MQ=255)

                    gaAAGCGCGCTTCGACAAGATGATTTCGTCCCAGGAAGTGGGCACGCTGATCACTGCGCTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTc                                                                                                                                  <  1:2127367/150‑1 (MQ=255)

                     aaaGCGCGCTTCGACAAGATGATTTCGTCCCAGGAAGTGGGCACGCTGATCACTGCGCTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCg                                                                                                                                 >  1:407007/1‑150 (MQ=255)

                          gcgcTTCGACAAGATGATTTCGTCCCAGGAAGTGGGCACGCTGATCACTGCGCTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACAt                                                                                                                            <  1:4762567/150‑1 (MQ=255)

                              ttCGACAAGATGATTTCGTCCCAGGAAGTGGGCACGCTGATCACTGCGCTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGt                                                                                                                        >  2:8136991/1‑150 (MQ=255)

                               tCGACAAGATGATTTCGTCCCAGGAAGTGGGCACGCTGATCACTGCGCTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTa                                                                                                                       <  2:4748285/150‑1 (MQ=255)

                                 gACAAGATGATTTCGTCCCAGGAAGTGGGCACGCTGATCACTGCGCTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACg                                                                                                                     >  1:2783563/1‑150 (MQ=255)

                                      gatgatTTCGTCCCAGGAAGTGGGCACGCTGATCACTGCGCTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACgctgct                                                                                                                >  1:2931466/1‑150 (MQ=255)

                                          atTTCGTCCCAGGAAGTGGGCACGCTGATCACTGCGCTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGctgctgctg                                                                                                            <  2:9081341/150‑1 (MQ=255)

                                          atTTCGTCCCAGGAAGTGGGCACGCTGATCACTGCGCTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGctgctgctg                                                                                                            <  1:3924239/150‑1 (MQ=255)

                                           tttCGTCCCAGGAAGTGGGCACGCTGATCACTGCGCTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGa                                                                                                           <  2:328025/150‑1 (MQ=255)

                                           tttCGTCCCAGGAAGTGGGCACGCTGATCACTGCGCTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGa                                                                                                           <  2:3147432/150‑1 (MQ=255)

                                           tttCGTCCCAGGAAGTGGGCACGCTGATCACTGCGCTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGa                                                                                                           >  2:1957563/1‑150 (MQ=255)

                                            ttCGTCCCAGGAAGTGGGCACGCTGATCACTGCGCTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGAc                                                                                                          >  2:5892883/1‑150 (MQ=255)

                                                  ccAGGAAGTGGGCACGCTGATCACTGCGCTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACcttctt                                                                                                    >  1:2035271/1‑150 (MQ=255)

                                                  ccAGGAAGTGGGCACGCTGATCACTGCGCTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACcttctt                                                                                                    >  1:241018/1‑150 (MQ=255)

                                                   cAGGAAGTGGGCACGCTGATCACTGCGCTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACcttcttc                                                                                                   <  1:3691971/150‑1 (MQ=255)

                                                        aGTGGGCACGCTGATCACTGCGCTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACctt                                                                                                       >  2:7416311/1‑141 (MQ=255)

                                                        aGTGGGCACGCTGATCACTGCGCTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACctt                                                                                                       <  1:7416311/141‑1 (MQ=255)

                                                         gTGGGCACGCTGATCACTGCGCTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGt                                                                                             >  2:8680996/1‑150 (MQ=255)

                                                             gCACACTGATCACTGCGCTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGc                                                                                         >  2:5313134/1‑150 (MQ=255)

                                                               aCGCTGATCACTGCGCTGGGCTGTGGCATAGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGAAGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTg                                                                                       >  1:8259798/1‑150 (MQ=255)

                                                                 gCTGATCACTGCGCTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGcc                                                                                     >  2:5087762/1‑150 (MQ=255)

                                                                    gATCACTGCGCTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGa                                                                                  <  1:7929875/150‑1 (MQ=255)

                                                                    gATCACTGCGCTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGa                                                                                  >  1:4302789/1‑150 (MQ=255)

                                                                         cTGCGCTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTgg                                                                             <  2:4460192/150‑1 (MQ=255)

                                                                         cTGCGCTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTgg                                                                             >  2:5313788/1‑150 (MQ=255)

                                                                            cgcTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTc                                                                           <  2:5855778/149‑1 (MQ=255)

                                                                               tGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGc                                                                       <  1:4495833/150‑1 (MQ=255)

                                                                                  gCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTg                                                                    >  2:1447800/1‑150 (MQ=255)

                                                                                  gCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTg                                                                    <  1:1656926/150‑1 (MQ=255)

                                                                                   cTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTgg                                                                   <  1:4914488/150‑1 (MQ=255)

                                                                                   cTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTgg                                                                   <  2:4302789/150‑1 (MQ=255)

                                                                                   cTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTgg                                                                   <  2:4224900/150‑1 (MQ=255)

                                                                                   cTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTgg                                                                   <  2:4224826/150‑1 (MQ=255)

                                                                                    tgtgGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTCCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGc                                                                  >  2:1105445/1‑150 (MQ=255)

                                                                                      tgGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTa                                                                >  2:6179323/1‑150 (MQ=255)

                                                                                       ggCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTAc                                                               <  2:1181980/150‑1 (MQ=255)

                                                                                       ggCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTAc                                                               <  1:597973/150‑1 (MQ=255)

                                                                                        gCATCGGCCGCGAGGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACa                                                              >  2:6841266/1‑150 (MQ=255)

                                                                                        gCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGAt                                                                                                                                                   >  1:1084115/1‑65 (MQ=255)

                                                                                          aTCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATc                                                            <  2:1597272/150‑1 (MQ=255)

                                                                                           tCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCt                                                           <  2:6391846/150‑1 (MQ=255)

                                                                                                  cgAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGc                                                    >  1:6039209/1‑150 (MQ=255)

                                                                                                     agagTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCa                                                 <  2:6335653/150‑1 (MQ=255)

                                                                                                         tACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAgccg                                             >  2:2483280/1‑150 (MQ=255)

                                                                                                         tACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAgccg                                             >  2:6495101/1‑150 (MQ=255)

                                                                                                            aatcctgACAAGCTGCGCTACCATAAGATCATCATCATGACGGATGCTGACGTCGACGGTGCGCATATTCGTACGCTGCTGTTGACCTTCTTCTTCCGTCAGATGCCGGAACTGATCGAGCGTGGCCATATCTATATCGCGCAGccgccg                                          <  2:8056282/144‑1 (MQ=255)

                                                                                                            aacaTCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGccgccg                                          >  1:3815789/1‑150 (MQ=255)

                                                                                                             acaTCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCcgccgc                                         <  1:7554178/150‑1 (MQ=255)

                                                                                                              tcctgACAAGCTGCGCTACCATAAGATCATCATCATGACGGATGCTGACGTCGACGGTGCGCATATTCGTACGCTGCTGTTGACCTTCTTCTTCCGTCAGATGCCGGAACTGATCGAGCGTGGCCATATCTATATCGCGCAGCCGCCGCt                                        >  1:2850627/5‑150 (MQ=255)

                                                                                                               cctgACAAGCTGCGCTACCATAAGATCATCATCATGACGGATGCTGACGTCGACGGTGCGCATATTCGTACGCTGCTGTTGACCTTCTTCTTCCGTCAGATGCCGGAACTGATCGAGCGTGGCCATATCTATATCGCGCAGCCGCCGCtc                                       <  2:3218730/147‑2 (MQ=255)

                                                                                                               aTCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTg                                       >  1:7303616/1‑150 (MQ=255)

                                                                                                               aTCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCGGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTg                                       >  2:3142501/1‑150 (MQ=255)

                                                                                                                  gACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTaa                                    >  1:6411947/1‑149 (MQ=255)

                                                                                                                  gACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTAc                                    <  1:2856331/150‑1 (MQ=255)

                                                                                                                    cAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTACTTCGTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACaa                                  <  1:3361349/150‑1 (MQ=255)

                                                                                                                         tGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGa                             >  2:7213606/1‑150 (MQ=255)

                                                                                                                           cGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGaaa                           <  2:863001/150‑1 (MQ=255)

                                                                                                                            gTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAAc                          <  2:5566960/150‑1 (MQ=255)

                                                                                                                               aTCACAACCTCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAg                       <  2:8799652/150‑1 (MQ=255)

                                                                                                                               aTCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAg                       <  1:5332642/150‑1 (MQ=255)

                                                                                                                               aTCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAg                       <  2:849877/150‑1 (MQ=255)

                                                                                                                               aTCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAg                       <  2:238976/150‑1 (MQ=255)

                                                                                                                                 cacaACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGc                     <  1:1265703/150‑1 (MQ=255)

                                                                                                                                 cacaACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGc                     <  1:5102739/150‑1 (MQ=255)

                                                                                                                                  acaacaTCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCa                    >  2:183463/1‑150 (MQ=255)

                                                                                                                                  acaacaTCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCa                    >  1:3115567/1‑150 (MQ=255)

                                                                                                                                  acaacaTCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCa                    >  2:8522073/1‑150 (MQ=255)

                                                                                                                                  acaacaTCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCa                    >  2:1395459/1‑150 (MQ=255)

                                                                                                                                    aacaTCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAg                  >  1:4359938/1‑150 (MQ=255)

                                                                                                                                     acaTCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCGCAGCAGCCGCTGTACAATGTGAAACGAGGCAAGc                 >  2:2202945/1‑150 (MQ=255)

                                                                                                                                     acaTCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGc                 >  1:6810256/1‑150 (MQ=255)

                                                                                                                                      gatcatcatcatGACGGATGCTGACGTCGACGGTGCGCATATTCGTACGCTGCTGTTGACCTTCTTCTTCCGTCAGATGCCGGAACTGATCGAGCGTGGCCATATCTATATCGCGCAGCCGCCGCTCTATAAGGTGTCTCGCGGCAAgtc                >  2:2054562/2‑148 (MQ=255)

                                                                                                                                      catcatcatcatGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCa                >  2:7826326/1‑150 (MQ=255)

                                                                                                                                         catcatcatGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGa             <  2:407007/150‑1 (MQ=255)

                                                                                                                                           tcatcatGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGc           >  2:3948737/1‑150 (MQ=255)

                                                                                                                                            catcatGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCa          <  1:6200877/150‑1 (MQ=255)

                                                                                                                                              tcatGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGt        <  1:7826326/150‑1 (MQ=255)

                                                                                                                                                atGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGccc                                                  >  1:1571445/1‑106 (MQ=255)

                                                                                                                                                atGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGccc                                                  <  2:1571445/106‑1 (MQ=255)

                                                                                                                                                   aCCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATc   >  2:5542971/1‑150 (MQ=255)

                                                                                                                                                    ccGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCa  <  2:3928035/150‑1 (MQ=255)

GGTAAAATCCTCAACGTCGAGAAAGCGCGCTTCGACAAGATGATTTCGTCCCAGGAAGTGGGCACGCTGATCACTGCGCTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCA  >  NC_002947/14677‑14974

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`