Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,286,864 A→T 5.1% K81* (AAG→TAG)  PP_1125 → putative Helicase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,286,8640AT 5.1% 868.5 / 20.5 255K81* (AAG→TAG) PP_1125putative Helicase
Reads supporting (aligned to +/- strand):  ref base A (106/134);  new base T (9/5);  total (116/139)
Fisher's exact test for biased strand distribution p-value = 1.72e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 268 total aligned reads displayed.

TGATGATCGCCGAAGTGGCCAAGGTGCTTGGCGACATTGCCTGCGACGATGAAGGCCGCCGCGCTGGCGAGCCTGCCGTGGTGGCCGTGGAAGCGGGCACCGGTACCGGCAAGACGGTAGCCTACAGCCTGGCTGCCATCCCGGCGGCCAAGGCCGCCGGCAAACGCCTGGTGATCGCCACGGCCACCGTGGCCCTGCAGGAACAGATCGTCTTCAAGGACCTGCCCGACCTGATGCGCAGCAGCGGCCTCAACTTCAGCTTCGCCCTGGCCAAGGGCCGTGGCCGCTATCTGTGCCTG  >  NC_002947/1286715‑1287013
                                                                                                                                                     |                                                                                                                                                     
tgatgaTCGCCGAAGTGGCCAAGGTGCTTGGCGACATTGCCTGCGACGATGAAGGCCGCCGCGCTGGCGAGCCTGCCGTGGTGGCCGTGGAAGCGGGCACCGGTACCGGCAAGACGGTAGCCTACAGCCTGGCTGCCATCCCGGCGGCCa                                                                                                                                                       >  2:4197154/1‑150 (MQ=255)
 gatgatCGCCGAAGTGGCCAAGGTGCTTGGCGACATTGCCTGCGACGATGAAGGCCGCCGCGCTGGCGAGCCTGCCGTGGTGGCCGTGGAAGCGGGCACCGGTACCGGCAAGACGGTAGCCTACAGCCTGGCTGCCATCCCGGCGGCCaa                                                                                                                                                      >  2:8011186/1‑150 (MQ=255)
   tgatCGCCGAAGTGGCCAAGGTGCTTGGCGACATTGCCTGCGACGATGAAGGCCGCCGCGCTGGCGAGCCTGCCGTGGTGGCCGTGGAAGCGGGCACCGGTACCGGCAAGACGGTAGCCTACAGCCTGGCTGCCATCCCGGCGGCCAAgg                                                                                                                                                    >  1:5299358/1‑150 (MQ=255)
       cGCCGAAGTGGCCAAGGTGCTTGGCGACATTGCCTGCGACGATGAAGGCCGCCGCGCTGGCGAGCCTGCCGTGGTGGCCGTGGAAGCGGGCACCGGTACCGGCAAGACGGTAGCCTACAGCCTGGCTGCCATCCCGGCGGCCAAgctgta                                                                                                                                                <  1:6164635/150‑6 (MQ=255)
       cGCCGAAGTGGCCAAGGTGCTTGGCGACAGTGCCTGCGACGATGAAGGCCGCCGCGCTGGCGAGCCTGCCGTGGTGGCCGTGGAAGCGGGCACCGGTACCGGCAAGACGGTAGCCTACAGCCTGGCTGCCATCCCGGCGGCCAAgctgta                                                                                                                                                <  1:4455952/150‑6 (MQ=255)
         ccGAAGTGGCCAAGGTGCTTGGCGACATTGCCTGCGACGATGAAGGCCGCCGCGCTGGCGAGCCTGCCGTGGTGGCCGTGGAAGCGGGCACCGGTACCGGCAAGACGGTAGCCTACAGCCTGGCTGCCATCCCGGCGGCCAAGGccgccg                                                                                                                                              <  2:1534843/150‑1 (MQ=255)
          cGAAGTGGCCAAGGTGCTTGGCGACATTGCCTGCGACGATGAAGGCCGCCGCGCTGGCGAGCCTGCCGTGGTGGCCGTGGAAGCGGGCACCGGTACCGGCAAGACGGTAGCCTACAGCCTGGCTGCCATCCCGGCGGCCTTGGCCGCCgg                                                                                                                                             <  2:5695656/150‑1 (MQ=255)
          cGAAGTGGCCAAGGTGCTTGGCGACATTGCCTGCGACGATGAAGGCCGCCGCGCTGGCGAGCCTGCCGTGGTGGCCGTGGAAGCGGGCACCGGTACCGGCAAGACGGTAGCCTACAGCCTGGCTGCCATCCCGGCGGCCAAGGCCGCCgg                                                                                                                                             <  2:5186593/150‑1 (MQ=255)
          cGAAGTGGCCAAGGTGCTTGGCGACATTGCCTGCGACGATGAAGGCCGCCGCGCTGGCGAGCCTGCCGTGGTGGCCGTGGAAGCGGGCACCGGTACCGGCAAGACGGTAGCCTACAGCCTGGCTGCCATCCCGGCGGCCAAGGCCGCCgg                                                                                                                                             >  1:3519605/1‑150 (MQ=255)
            aaGTGGCCAAGGTGCTTGGCGACATTGCCTGCGACGATGAAGGCCGCCGCGCTGGCGAGCCTGCCGTGGTGGCCGTGGAAGCGGGCACCGGTACCGGCAAGACGGTAGCCTACAGCCTGGCTGCCATCCCGGCGGCCAAGGCCGCCGGCa                                                                                                                                           >  1:1154764/1‑150 (MQ=255)
                 gCCAAGGTGCTTGGCGACATTGCCTGCGACGATGAAGGCCGCCGCGCTGGCGAGCCTGCCGTGGTGGCCGTGGAAGCGGGCACCGGTACCGGCAAGACGGTAGCCTACAGCCTGGCTGCCATCCCGGCGGCCAAGGCCGCCGGCAAACGc                                                                                                                                      <  1:657142/150‑1 (MQ=255)
                  ccAAGGTGCTTGGCGACATTGCCTGCGACGATGAAGGCCGCCGCGCTGGCGAGCCTGCCGTGGTGGCCGTGGAAGCGGGCACCGGTACCGGCAAGACGGTAGCCTACAGCCTGGCTGCCATCCCGGCGGCCAAGGCCGCCGGCAAACGcc                                                                                                                                     >  2:4834636/1‑150 (MQ=255)
                    aaGGTGCTTGGCGACATTGCCTGCGACGATGAAGGCCGCCGCGCTGGCGAGCCTGCCGTGGTGGCCGTGGAAGCGGGCACCGGTACCGGCAAGACGGTAGCCTACAGCCTGGCTGCCATCCCGGCGGCCAAGGCCGCCGGCAAACGCCTg                                                                                                                                   >  1:4080601/1‑150 (MQ=255)
                      ggTGCTTGGCGACATTGCCTGCGACGATGAAGGCCGCCGCGCTGGCGAGCCTGCCGTGGTGGCCGTGGAAGCGGGCACCGGTACCGGCAAGACGGTAGCCTACAGCCTGGCTGCCATCCCGGCGGCCAAGGCCGCCGGCAAACGCCTGGt                                                                                                                                 <  2:2788704/150‑1 (MQ=255)
                          cTTGGCGACATTGCCTGCGACGATGAAGGCCGCCGCGCTGGCGAGCCTGCCGTGGTGGCCGTGGAAGCGGGCACCGGTACCGGCAAGACGGTAGCCTACAGCCTGGCTGCCATCCCGGCGGCCAAGGCCGCCGGCAAACGCCTGGTGATc                                                                                                                             <  2:1336944/150‑1 (MQ=255)
                          cTTGGCGACATTGCCTGCGACGATGAAGGCCGCCGCGCTGGCGAGCCTGCCGTGGTGGCCGTGGAAGCGGGCACCGGTACCGGCAAGACGGTAGCCTACAGCCTGGCTGCCATCCCGGCGGCCAAGGCCGCCGGCAAACGCCTGGTGATc                                                                                                                             <  2:3470108/150‑1 (MQ=255)
                           ttGGCGACATTGCCTGCGACGATGAAGGCCGCCGCGCTGGCGAGCCTGCCGTGGTGGCCGTGGAAGCGGGCACCGGTACCGGCAAGACGGTAGCCTACAGCCTGGCTGCCATCCCGGCGGCCAAGGCCGCCGGCAAACGCCTGGTGATCg                                                                                                                            >  2:6697162/1‑150 (MQ=255)
                                gACATTGCCTGCGACGATGAAGGCCGCCGCGCTGGCGAGCCTGCCGTGGTGGCCGTGGAAGCGGGCACCGGTACCGGAAAGACGGTAGCCTACAGCCTGGCTGCCATCCCGGCGGCCAAGGCCGCCGGCAAACGCCTGGTGATCGCCACg                                                                                                                       <  1:6216862/150‑1 (MQ=255)
                                     tGCCTGCGACGATGAAGGCCGCCGCGCTGGCGAGCCTGCCGTGGTGGCCGTGGAAGCGGGCACCGGTACCGGCAAGACGGTAGCCTACAGCCTGGCTGCCATCCCGGCGGCCAAGGCCGCCGGCAAACGCCTGGTGATCGCCACGGCCAc                                                                                                                  >  2:8585697/1‑150 (MQ=255)
                                     tGCCTGCGACGATGAAGGCCGCCGCGCTGGCGAGCCTGCCGTGGTGGCCGTGGAAGCGGGCACCGGTACCGGCAAGACGGTAGCCTACAGCCTGGCTGCCATCCCGGCGGCCAAGGCCGCCGGCAAACGCCTGGTGATCGCCACGGCCAc                                                                                                                  <  2:7357823/150‑1 (MQ=255)
                                      gCCTGCGACGATGAAGGCCGCCGCGCTGGCGAGCCTGCCGTGGTGGCCGTGGAAGCGGGCACCGGTACCGGCAAGACGGTAGCCTACAGCCTGGCTGCCATCCCGGCGGCCAAGGCCGCCGGCAAACGCCTGGTGATCGCCACGGCCAcc                                                                                                                 <  2:4202650/150‑1 (MQ=255)
                                        cTGCGACGATGAAGGCCGCCGCGCTGGCGAGCCTGCCGTGGTGGCCGTGGAAGCGGGCACCGGTACCGGCAAGACGGTAGCCTACAGCCTGGCTGCCATCCCGGCGGCCAAGGCCGCCGGCAAACGCCTGGTGATCGCCACGGCCACCGt                                                                                                               >  2:6386138/1‑150 (MQ=255)
                                         tGCGACGATGAAGGCCGCCGCGCTGGCGAGCCTGCCGTGGTGGCCGTGGAAGCGGGCACCGGTACCGGCAAGACGGTAGCCTACAGCCTGGCTGCCATCCCGGCGGCCAAGGCCGCCGGCAAACGCCTGGTGATCGCCACGGCCACCGTg                                                                                                              <  1:4695621/150‑1 (MQ=255)
                                          gCGACGATGAAGGCCGCCGCGCTGGCGAGCCTGCCGTGGTGGCCGTGGAAGCGGGCACCGGTACCGGCAAGACGGTAGCCTACAGCCTGGCTGCCATCCCGGCGGCCAAGGCCGCCGGCAAACGCCTGGTGATCGCCACGGCCACCGTgg                                                                                                             <  1:8081700/150‑1 (MQ=255)
                                          gCGACGATGAAGGCCGCCGCGCTGGCGAGCCTGCCGTGGTGGCCGTGGAAGCGGGCACCGGTACCGGCAAGACGGTAGACTACAGCCTGGCTGCCATCCCGGCGGCCAAGGCCGCCGGCAAACGCCTGGTGATCGCCACGGCCACCGTgg                                                                                                             >  2:707718/1‑150 (MQ=255)
                                               gaTGAAGGCCGCCGCGCTGGCGAGCCTGCCGTGGTGGCCGTGGAAGCGGGCACCGGTACCGGCAAGACGGTAGCCTACAGCCTGGCTGCCATCCCGGCGGCCAAGGCCGCCGGCAAACGCCTGGTGATCGCCACGGCCACCGTGGCCCTg                                                                                                        >  1:2364352/1‑150 (MQ=255)
                                               gaTGAAGGCCGCCGCGCTGGCGAGCCTGCCGTGGTGGCCGTGGAAGCGGGCACCGGTACCGGCAAGACGGTAGCCTACAGCCTGGCTGCCATCCCGGCGGCCAAGGCCGCCGGCAAACGCCTGGTGATCGCCACGGCCACCGTGGCCCTg                                                                                                        >  2:754699/1‑150 (MQ=255)
                                                 tGAAGGCCGCCGCGCTGGCGAGCCTGCCGTGGTGGCCGTGGAAGCGGGCACCGGTACCGGCAAGACGGTAGCCTACAGCCTGGCTGCCATCCCGGCGGCCAAGGCCGCCGGCAAACGCCTGGTGATCGCCACGGCCACCGTGGCCCTGCa                                                                                                      <  1:3318324/150‑1 (MQ=255)
                                                  gAAGGCCGCCGCGCTGGCGAGCCTGCCGTGGTGGCCGTGGAAGCGGGCACCGGTACCGGCAAGACGGTAGCCTACAGCCTGGCTGCCATCCCGGCGGCCAAGGCCGCCGGCAAACGCCTGGTGATCGCCACGGCCACCGTGGCCCTGCAg                                                                                                     >  1:2745364/1‑150 (MQ=255)
                                                  gAAGGCCGCCGCGCTGGCGAGCCTGCCGTGGGGGCCGTGGAAGCGGGCACCGGTACCGGCAAGACGGTAGCCTACAGCCTGGCTGCCATCCCGGCGGCCAAGGCCGCCGGCAAACGCCTGGTGATCGCCACGGCCACCGTGGCCCTGCAg                                                                                                     <  1:1750894/150‑1 (MQ=255)
                                                    aGGCCGCCGCGCTGGCGAGCCTGCCGTGGTGGCCGTGGAAGCGGGCACCGGTACCGGCAAGACGGTAGCCTACAGCCTGGCTGCCAGCCCGGCGGCCAAGGCCGCCGGCAAACGCCTGGTGATCGCCACGGCCACCGTGGCCCTGCAGGa                                                                                                   <  2:2398910/150‑1 (MQ=255)
                                                           cgcgCTGGCGAGCCTGCCGTGGTGGCCGTGGAAGCGGGCACCGGTACCGGCAAGACGGTAGCCTACAGCCTGGCTGCCATCCCGGCGGCCAAGGCCGCCGGCAAACGCCTGGTGATCGCCACGGCCACCGTGGCCCTGCAGGAACAGATc                                                                                            <  1:2936860/150‑1 (MQ=255)
                                                                 ggCGAGCCTGCCGTGGTGGCCGTGGAAGCGGGCACCGGTACCGGCAAGACGGTAGCCTACAGCCTGGCTGCCATCCCGGCTGCCTAGGCCGCCGGCAAACGCCTGGTGATCGCCACGGCCACCGTGGCCCTGCAGGAACAGATCGTCTTc                                                                                      <  2:1096855/150‑1 (MQ=255)
                                                                 ggCGAGCCTGCCGTGGTGGCCGTGGAAGCGGGCACCGGTACCGGCAAGACGGTAGCCTACAGCCTGGCTGCCATCCCGGCGGCCAAGGCCGCCGGCAAACGCCTGGTGATCGCCACGGCCACCGTGGCCCTGCAGGAACAGATCGTCTTc                                                                                      <  2:1869326/150‑1 (MQ=255)
                                                                    gAGCCTGCCGTGGTGGCCGTGGAAGCGGGCACCGGTACCGGCAAGACGGTAGCCTACAGCCTGGCTGCCATCCCGGCGGCCAAGGCCGCCGGCAAACGCCTGGTGATCGCCACGGCCACCGTGGCCCTGCAGGAACGGATCGTCTTCAAg                                                                                   <  1:1961278/150‑1 (MQ=255)
                                                                       ccTGCCGTGGTGGCCGTGGAAGCGGGCACCGGTACCGGCAAGACGGTAGCCTACAGCCTGGCTGCCATCCCGGCGGCCAAGGCCGCCGGCAAACGCCTGGTGATCGCCACGGCCACCGTGGCCCTGCAGGAACAGATCGTCTTCAAGGAc                                                                                >  1:902521/1‑150 (MQ=255)
                                                                        cTGCCGTGGTGGCCGTGGAAGCGGGCACCGGTACCGGCAAGACGGTAGCCTACAGCCTGGCTGCCATCCCGGCGGCCAAGGCCGCCGGCAAACGCCTGGTGATCGCCACGGCCACCGTGGCCCTGCAGGAACAGATCGTCTTCAAGGAcc                                                                               >  1:6982880/1‑150 (MQ=255)
                                                                         tGCCGTGGTGGCCGTGGAAGCGGGCACCGGTACCGGCAAGACGGTAGCCTACAGCCTGGCTGCCATCCCGGCGGCCAAGGCCGCCGGCAAACGCCTGGTGATCGCCACGGCCACCGTGGCCCTGCAGGAACAGATCGTCTTCAAGGACCt                                                                              >  2:7128929/1‑150 (MQ=255)
                                                                           ccGTGGTGGCCGTGGAAGCGGGCACCGGTACCGGCAAGACGGTAGCCTACAGCCTGGCTGCCATCCCGGCGGCCAAGGCCGCCGGCAAACGCCTGGTGATCGCCACGGCCACCGTGGCCCTGCAGGAACAGATCGTCTTCAAGGACCTGc                                                                            <  1:5423556/150‑1 (MQ=255)
                                                                           ccGTGGTGGCCGTGGAAGCGGGCACCGGTACCGGCAAGACGGTAGCCTACAGCCTGGCTGCCATCCCGGCGGCCAAGGCCGCCGGCAAACGCCTGGTGATCGCCACGGCCACCGTGGCCCTGCAGGAACAGATCGTCTTCAAGGACCTGc                                                                            >  2:8342700/1‑150 (MQ=255)
                                                                             gtggtgGCGGTGGACGCGGGCACCGGTCCCGGAAAGAGGGTAGCAAACCGCCGGCCCGCAAGCCGGGCGCCCAAGGCCGCCGGCAAACGCCTGGTGATCGCCACGGCCACCGTGGCCCTGCAGGAACAGATCGTCTTCAAGGACCTGccc                                                                          <  1:1909369/150‑1 (MQ=255)
                                                                             gtggtgGCCGTGGAAGCGGGCACCGGTACCGGCAAGACGGTAGCCTACAGCCTGGCTGCCATCCCGGCGGCCAAGGCCGCCGGCAAACGCCTGGTGATCGCCACGGCCACCGTGGCCCTGCAGGAACAGATCGTCTTCAAGGACCTGccc                                                                          <  1:4926247/150‑1 (MQ=255)
                                                                             agtgtggCCGTGGAAGCGGGCACCGGTACCGGCAAGACGGTAGCCTACAGCCTGGCTGCCATCCCGGCGGCCAAGGCCGCCGGCAAACGCCTGGTGATCGCCACGGCCACCGTGGCCCTGCAGGAACAGATCGTCTTCAAGGACCTGccc                                                                          >  2:4297921/4‑150 (MQ=255)
                                                                                gtggCCGTGGAAGCGGGCACCGGTACCGGCAAGACGGTAGCCTACAGCCTGGCTGCCATCCCGGCGGCCAAGGCCGCCGGCAAACGCCTGGTGATCGCCACGGCCACCGTGGCCCTGCAGGAACAGATCGTCTTCAAGGACCTGCCCGAc                                                                       <  2:7763734/150‑1 (MQ=255)
                                                                                gtggCCGTGGAAGCGGGCACCGGTACCGGCAAGACGGTAGCCTACAGCCTGGCTGCCATCCCGGCGGCCAAGGCCGCCGGCAAAAGCCTGGTGATCGCCACGGCCACCGTGGCCCTGCAGGAACAGATCGTCTTCAAGGACCTGCCCGAc                                                                       <  1:986468/150‑1 (MQ=255)
                                                                                 tggCCGTGGAAGCGGGCACCGGTACCGGCAAGACGGTAGCCTACAGCCTGGCTGCCATCCCGGCGGCCAAGGCCGCCGGCAAACGCCTGGTGATCGCCACGGCCACCGTGGCCCTGCAGGAACAGATCGTCTTCAAGGACCTGCCCGAcc                                                                      <  2:5825986/150‑1 (MQ=255)
                                                                                      gTGGAAGCGGGCACCGGTACCGGCAAGACGGTAGCCTACAGCCTGGCTGCCATCCCGGCGGCCAAGGCCGCCGGCAAACGCCTGGTGATCGCCACGGCCACCGTGGCCCTGCAGGAACAGATCGTCTTCAAGGACCTGCCCGACCTGATg                                                                 >  1:8791471/1‑150 (MQ=255)
                                                                                       tGGAAGCGGGCACCGGTACCGGCAAGACGGTAGCCTACAGCCTGGCTGCCATCCCGGCGGCCAAGGCCGCCGGCAAACGCCTGGTGATCGCCACGGCCACCGTGGCCCTGCAGGAACAGATCGTCTTCAAGGACCTGCCCGACCTGATgc                                                                <  2:8843519/150‑1 (MQ=255)
                                                                                       tGGAAGCGGGCACCGGTACCGGCAAGACGGTAGCCTACAGCCTGGCTGCCATCCCGGCGGCCAAGGCCGCCGGCAAACGCCTGGTGATCGCCACGGCCACCGTGGCCCTGCAGGAACAGATCGTCTTCAAGGACCTGCCCGACCTGATgc                                                                >  1:706929/1‑150 (MQ=255)
                                                                                        ggAAGCGGGCACCGGTACCGGCAAGACGGTAGCCTACAGCCTGGCTGCCATCCCGGCGGCCAAGGCCGCCGGCAAACGCCTGGTGATCGCCACGGCCACCGTGGCCCTGCAGGAACAGATCGTCTTCAAGGACCTGCCCGACTTGATgcg                                                               >  1:4885320/1‑150 (MQ=255)
                                                                                             cGGGCACCGGTACCGGCAAGACGGTAGCCTACAGCCTGGCTGCCATCCCGGCGGCCAAGGCCGCCGGCAAACGCCTGGTGATCGCCACGGCCACCGTGGCCCTGCAGGAACAGATCGTCTTCAAGGACCTGCCCGACCTGATGCgcagca                                                          <  1:9146216/150‑1 (MQ=255)
                                                                                               ggCACCGGTACCGGCAAGACGGTAGCCTACAGCCTGGCTGCCATCCCGGCGGCCAAGGCCGCCGGCAAACGCCTGGTGATCGCCACGGCCACCGTGGCCCTGCAGGAACAGATCGTCTTCAAGGACCTGCCCGACCTGATGCGCagcagc                                                        <  1:2541697/150‑1 (MQ=255)
                                                                                               ggCACCGGTACCGGCAAGACGGTAGCCTACAGCCTGGCTGCCATCCCGGCGGCCAAGGCCGCCGGCAAACGCCTGGTGATCGCCACGGCCACCGTGGCCCTGCAGGAACAGATCGTCTTCAAGGACCTGCCCGACCTGATGCGCagcagc                                                        <  2:8520932/150‑1 (MQ=255)
                                                                                                gCACCGGTACCGGCAAGACGGTAGCCTACAGCCTGGCTGCCATCCCGGCGGCCAAGGCCGCCGGCAAACGCCTGGTGATCGCCACGGCCACCGTGGCCCTGCAGGAACAGATCGTCTTCAAGGACCTGCCCGACCTGATGCGCAGCAGCg                                                       >  2:4002754/1‑150 (MQ=255)
                                                                                                  aCCGGTACCGGCAAGACGGTAGCCTACAGCCTGGCTGCCATCCCGGCGGCCAAGGCCGCCGGCAAACGCCTGGTGATCGCCACGGCCACCGTGGCCCTGCAGGAACAGATCGTCTTCAAGGACCTGCCCGACCTGATGCGCAGCAGCGGc                                                     >  1:5590388/1‑150 (MQ=255)
                                                                                                  aCCGGTACCGGCAAGACGGTAGCCTACAGCCTGGCTGCCATCCCGGCGGCCAAGGCCGCCGGCAAACGCCTGGTGATCGCCACGGCCACCGTGGCCCTGCAGGAACAGATCGTCTTCAAGGACCTGCCCGACCTGATGCGCAGCAGCGGc                                                     <  1:3607431/150‑1 (MQ=255)
                                                                                                    cGGTACCGGCAAGACGGTAGCCTACAGCCTGGCTGCCATCCCGGCGGCCAAGGCCGCCGGCAATCGCCTGGTGATCTCCACGGCCACCGTGGCCCTGGAGGAACAGATCGTCTTCAAGGACCTGCCCGACCTGATGCGCAGCAGCGGCCt                                                   <  1:6725386/150‑1 (MQ=255)
                                                                                                     ggTACCGGCAAGACGGTAGCCTACAGCCTGGCTGCCATCCCGGCGGCCAAGGCCGCCGGCAAACGCCTGGTGATCGCCACGGCCACCGTGGCCCTGCAGGAACAGATCGTCTTCAAGGACCTGCCCGACCTGATGCGCAGCAGCGGCCTc                                                  <  1:7633594/150‑1 (MQ=255)
                                                                                                       gaCCGGCAAGACGGTAGCCGACAGCCTGGCTGCCATCCCGGCGGCCAAGGCCGCCGGCAAACGCCTGGTGATCGCCACGGCCACCGTGGCCCTGCAGGAACAGATCGTCTTCAAGGACCTGCCCGACCTGATGCGCAGCAGCGGCCTCaa                                                <  2:6043574/149‑1 (MQ=255)
                                                                                                          cGGCAAGACGGTAGCCTACAGCCTGGCTGCCATCCCGGCGGCCAAGGCCGCCGGCAAACGCCTGGTGATCGCCACGGCCACCGTGGCCCTGCAGGAACAGATCGTCTTCAAGGACCTGCCCGACCTGATGCGCAGCAGCGGCCTCAACtt                                             >  2:4540785/1‑150 (MQ=255)
                                                                                                            gCAAGACGGTAGCCTACAGCCTGGCTGCCATCCCGGCGGCCAAGGCCGCCGGCAAACGCCTGGTGATCGCCACGGCCACCGTGGCCCTGCAGGAACAGATCGTCTTCAAGGACCTGCCCGACCTGATGCGCAGCAGCGGCCTCAACTTCa                                           <  1:8000212/150‑1 (MQ=255)
                                                                                                              aaGACGGTAGCCTACAGCCTGGCTGCCATCCCGGCGGCCAAGGCCGCCGGCAAACGCCTGGTGATCGCCACGGCCACCGTGGCCCTGCAGGAACAGATCGTCTTCAAGGACCTGCCCGACCTGATGCGCAGCAGCGGCCTCAACTTCAGc                                         >  2:1249289/1‑150 (MQ=255)
                                                                                                                 aCGGTAGCCTACAGCCTGGCTGCCATCCCGGCGGCCAAGGCCGCCGGCAAACGCCTGGTGATCGCCACGGCCACCGTGGCCCTGCAGGAACAGATCGTCTTCAAGGACCTGCCCGACCTGATGCGCAGCAGCGGCCTCAACTTCAGCTTc                                      <  1:1008003/150‑1 (MQ=255)
                                                                                                                 aCGGTAGCCTACAGCCTGGCTGCCATCCCGGCGGCCAAGGCCGCCGGCAAACGCCTGGTGATCGCCACGGCCACCGTGGCCCTGCAGGAACAGATCGTCTTCAAGGACCTGCCCGACCTGATGCGCAGCAGCGGCCTCAACTTCAGCTTc                                      <  2:2409741/150‑1 (MQ=255)
                                                                                                                 aCGGTAGCCTACAGCCTGGCTGCCATCCCGGCGGCCAAGGCCGCCGGCAAACGCCTGGTGATCGCCACGGCCACCGTGGCCCTGCAGGAACAGATCGTCTTCAAGGACCTGCCCGACCTGATGCGCAGCAGCGGCCTCAACTTCAGCTTc                                      <  2:5775029/150‑1 (MQ=255)
                                                                                                                  cGGTAGCCTACAGCCTGGCTGCCATCCCGGCGGCCAAGGCCGCCGGCAAACGCCTGGTGATCGCCACGGCCACAGTGGCCCTGCAGGAACAGATCGTCTTCAAGGACCTGCCCGACCTGATGCGCAGCAGCGGCCTCAACTTCAGCTTCg                                     <  1:8658171/150‑1 (MQ=255)
                                                                                                                     tAGCCTACAGCCTGGCTGCCATCCCGGCGGCCAAGGCCGCCGGCAAACGCCTGGTGATCGCCACGGCCACCGTGGCCCTGCAGGAACAGATCGTCTTCAAGGACCTGCCCGACCTGATGCGCAGCAGCGGCCTCAACTTCAGCTTCGccc                                  >  1:6828962/1‑150 (MQ=255)
                                                                                                                       gCCTACAGCCTGGCTGCCATCCCGGCGGCCAAGGCCGCCGGCAAACGCCTGGTGATCGCCACGGCCACCGTGGCCCTGCAGGAACAGATCGTCTTCAAGGACCTGCCCGACCTGATGCGCAGCAGCGGCCTCAACTTCAGCTTCGCCCTg                                <  1:8751079/150‑1 (MQ=255)
                                                                                                                        ccTACAGCCTGGCTGCCATCCCGGCGGCCAAGGCCGCCGGCAAACGCCTGGTGATCGCCACGGCCACCGTGGCCCTGCAGGAACAGATCGTCTTCAAGGACCTGCCCGACCTGATGCGCAGCAGCGGCCTCAACTTCAGCTTCGCCCTgg                               >  1:1527797/1‑150 (MQ=255)
                                                                                                                         cTACAGCCTGGCTGCCATCCCGGCGGCCAAGGCCGCCGGCAAACGCCTGGTGATCGCCACGGCCACCGTGGCCCTGCAGGAACAGATCGTCTTCAAGGACCTGCCCGACCTGATGCGCAGCAGCGGCCTCAACTTCAGCTTCGCCCTGGc                              <  2:55242/150‑1 (MQ=255)
                                                                                                                         cTACAGCCTGGCTGCCATCCCGGCGGCCAAGGCCGCCGGCAAACGCCTGGTGATCGCCACGGCCACCGTGGCCCTGCAGGAACAGATCGTCTTCAAGGACCTGCCCGACCTGATGCGCAGCAGCGGCCTCAACTTCAGCTTCGCCCTGGc                              >  2:5158867/1‑150 (MQ=255)
                                                                                                                         cTACAGCCTGGCTGCCATCCCGGCGGCCAAGGCCGCCGGCAAACGCCTGGTGATCGCCACGGCCACCGTGGCCCTGCAGGAACAGATCGTCTTCAAGGACCTGCCCGACCTGATGCGCAGCAGCGGCCTCAACTTCAGCTTCGCCCTGGc                              >  1:3162527/1‑150 (MQ=255)
                                                                                                                          tACAGCCTGGCTGCCATCCCGGCGGCCAAGGCCGCCGGCAAACGCCTGGTGATCGCCACGGCCACCGTGGCCCTGCAGGAACAGATCGTCTTCAAGGACCTGCCCGACCTGATGCGCAGCAGCGGCCTCAACTTCAGCTTCGCCCTGGcc                             >  1:7084422/1‑150 (MQ=255)
                                                                                                                           aCAGCCTGGCTGCCATCCCGGCGGCCAAGGCCGCCGGCAAACGCCTGGTGATCGCCACGGCCACCGTGGCCCTGCAGGAACAGATCGTCTTCAAGGACCTGCCCGACCTGATGCGCAGCAGCGGCCTCAACTTCAGCTTCGCCCTGGCCa                            >  1:1079225/1‑150 (MQ=255)
                                                                                                                               ccTGGCTGCCATCCCGGCGGCCAAGGCCGCCGGCAAACGCCTGGTGATCGCCACGGCCACCGTGGCCCTGCAGGAACAGATCGTCTTCAAGGACCTGCCCGACCTGATGCGCAGCAGCGGCCTCAACTTCAGCTTCGCCCTGGCCACggc                        <  1:7926622/150‑2 (MQ=255)
                                                                                                                                cTGGCTGCCATCCCGGCGGCCAAGGCCGCCGGCAAACGCCTGGTGATCGCCACGGCCACCGTGGCCCTGCAGGAACAGATCGTCTTCAAGGACCTGCCCGACCTGATGCGCAGCAGCGGCCTCAACTTCAGCTTCGCCCTGGCCAAGGGc                       >  1:5776782/1‑150 (MQ=255)
                                                                                                                                   gCTGCCATCCCGGCGGCCAAGGCCGCCGGCAAACGCCTGGTGATCGCCCCGGCCACCGTGGCCCTGCAGGAACAGATCGTCTTCAAGGACCTGCCCGACCTGATGCGCAGCAGCGGCCTCAACTTCAGCTTCGCCCTGGCCTAGGGCCGt                    <  2:8942745/150‑1 (MQ=255)
                                                                                                                                   gCTGCCATCCCGGCGGCCAAGGCCGCCGGCAAACGCCTGGTGATCGCCACGGCCACCGTGGCCCTGCAGGAACAGATCGTCTTCAAGGACCTGCCCGACCTGATGCGCAGCAGCGGCCTCAACTTCAGCTTCGCCCTGGCCAAGGGCCGt                    <  1:7581554/150‑1 (MQ=255)
                                                                                                                                     tGCCATCCCGGCGGCCAAGGCCGCCGGCAAACGCCTGGTGATCGCCACGGCCACCGTGGCCCTGCAGGAACAGATCGTCTTCAAGGACCTGCCCGACCTGATGCGCAGCAGCGGCCTCAACTTCAGCTTCGCCCTGGCCAAGGGCCGTgg                  <  2:807868/150‑1 (MQ=255)
                                                                                                                                        cATCCCGGCGGCCAAGGCCGCCGGCAAACGCCTGGTGATCGCCACGGCCACCGTGGCCCTGCAGGAACAGATCGTCTTCAAGGACCTGCCCGACCTGATGCGCAGCAGCGGCCTCAACTTCAGCTTCGCCCTGGCCAAGGGCCGTGGCCg               >  2:698324/1‑150 (MQ=255)
                                                                                                                                        cATCCCGGCGGCCAAGGCCGCCGGCAAACGCCTGGTGATCGCCACGGCCACCGTGGCCCTGCAGGAACAGATCGTCTTCAAGGACCTGCCCGACCTGATGCGCAGCAGCGGCCTCAACTTCAGCTTCGCCCTGGCCAAGGGCCGTGGCCg               >  2:3322470/1‑150 (MQ=255)
                                                                                                                                         aTCCCGGCGGCCAAGGCCGCCGGCAAACGCCTGGTGATCGCCACGGCCACCGTGGCCCTGCAGGAACAGATCGTCTTCAAGGACCTGCCCGACCTGATGCGCAGCAGCGGCCTCAACTTCAGCTTCGCCCTGGCCAAGGGCCGTGGCCGc              >  2:5668090/1‑150 (MQ=255)
                                                                                                                                         aTCCCGGCGGCCAAGGCCGCCGGCAAACGCCTGGTGATCGCCACGGCCACCGTGGCCCTGCAGGAACAGATCGTCTTCAAGGACCTGCCCGACCTGATGCGCAGCAGCGGCCTCAACTTCAGCTTCGCCCTGGCCAAGGGCCGTGGCCGc              >  1:5387749/1‑150 (MQ=255)
                                                                                                                                          tCCCGGCGGCCAAGGCCGCCGGCAAACGCCTGGTGATCGCCACGGCCACCGTGGCCCTGCAGGAACAGATCGTCTTCAAGGACCTGCCCGACCTGATGCGCAGCAGCGGCCTCAACTTCAGCTTCGCCCTGGCCAAGGGCCGTGGCCGCt             >  1:2751610/1‑150 (MQ=255)
                                                                                                                                          tCCCGGCGGCCAAGGCCGCCGGCAAACGCCTGGTGATCGCCACGGCCACCGTGGCCCTGCAGGAACAGATCGTCTTCAAGGACCTGCCCGACCTGATGCGCAGCAGCGGCCTCAACTTCAGCTTCGCCCTGGCCAAGGGCCGTGGCCGCt             >  1:3940803/1‑150 (MQ=255)
                                                                                                                                          tCCCGGCGGCCAAGGCCGCCGGCAAACGCCTGGTGATCGCCACGGCCACCGTGGCCCTGCAGGAACAGATCGTCTTCAAGGACCTGCCCGACCTGATGCGCAGCAGCGGCCTCAACTTCAGCTTCGCCCTGGCCAAGGGCCGTGGCCGCt             >  1:4499272/1‑150 (MQ=255)
                                                                                                                                             cggcggCCTTGGCCGCCGGCAAACGCCTGGTGATCGCCACGGCCACCGTGGCCCTGCAGGAACAGATCGTCTTCAAGGACCTGCCCGACCTGATGCGCAGCAGCGGCCTCAACTTCAGCTTCGCCCTGGCCAAGGGCCGTGGCCGCTATc          >  1:7307353/1‑150 (MQ=255)
                                                                                                                                             cggcggCCTTGGCCGCCGGCAAACGCCTGGTGATCGCCACGGCCACCGTGGCCCTGCAGGAACAGATCGTCTTCAAGGACCTGCCCGACCTGATGCGCAGCAGCGGCCTCAACTTCAGCTTCGCCCTGGCCAAGGGCCGTGGCCGCTATc          >  1:6909027/1‑150 (MQ=255)
                                                                                                                                             cggcggCCAAGGCCGCCGGCAAACGCCTGGTGATCGCCACGGCCACCGTGGCCCTGCAGGAACAGATCGTCTTCAAGGACCTGCCCGACCTGATGCGCAGCAGCGGCCTCAACTTCAGCTTCGCCCTGGCCAAGGGCCGTGGCCGCTATc          >  1:8376684/1‑150 (MQ=255)
                                                                                                                                             cggcggCCAAGGCCGCCGGCAAACGCCTGGTGATCGCCACGGCCACCGTGGCCCTGCAGGAACAGATCGTCTTCAAGGACCTGCCCGACCTGATGCGCAGCAGCGGCCTCAACTTCAGCTTCGCCCTGGCCAAGGGCCGTGGCCGCTATc          >  1:3145576/1‑150 (MQ=255)
                                                                                                                                              ggcggcCAAGGCCGCCGGCAAACGCCTGGTGATCGCCACGGCCACCGTGGCCCTGCAGGAACAGATCGTCTTCAAGGACCTGCCCGACCTGATGCGCAGCAGCGGCCTCAACTTCAGCTTCGCCCTGGCCAAGGGCCGTGGCCGCTATCt         <  1:4245826/150‑1 (MQ=255)
                                                                                                                                              ggcggcCAAGGCCGCCGGCAAACGCCTGGTGATCGCCACGGCCACCGTGGCCCTGCAGGAACAGATCGTCTTCAAGGACCTGCCCGACCTGATGCGCAGCAGCGGCCTCAACTTCAGCTTCGCCCTGGCCAAGGGCCGTGGCCGCTATCt         >  2:1664745/1‑150 (MQ=255)
                                                                                                                                                cggcCAAGGCCGCCGGCAAACGCCTGGTGATCGCCACGGCCACCGTGGCCCTGCAGGAACAGATCGTCTTCAAGGACCTGCCCGACCTGATGCGCAGCAGCGGCCTCAACTTCAGCTTCGCCCTGGCCAAGGGCCGTGGCCGCTATCtgt       <  2:5299358/150‑1 (MQ=255)
                                                                                                                                                cggcCAAGGCCGCCGGCAAACGCCTGGTGATCGCCACGGCCACCGTGGCCCTGCAGGAACAGATCGTCTTCAAGGACCTGCCCGACCTGATGCGCAGCAGCGGCCTCAACTTCAGCTTCGCCCTGGCCAAGGGCCGTGGCCGCTATCtgt       <  1:4197236/150‑1 (MQ=255)
                                                                                                                                                  gcCAAGGCCGCCGGCAAACGCCTGGTGATCGCCACGGCCACCGTGGCCCTGCAGGAACAGATCGTCTTCAAGGACCTGCCCGACCTGATGCGCAGCAGCGGCCTCAACTTCAGCTTCGCCCTGGCCAAGGGCCGTGGCCGCTATCTGTGc     <  2:4047907/150‑1 (MQ=255)
                                                                                                                                                  gcCAAGGCCGCCGGCAAACGCCTGGTGATCGCCACGGCCACCGTGGCCCTGCAGGAACAGATCGTCTTCAAGGACCTGCCCGACCTGATGCGCAGCAGCGGCCTCAACTTCAGCTTCGCCCTGGCCAAGGGCCGTGGCCGCTATCTGTGc     <  1:3619479/150‑1 (MQ=255)
                                                                                                                                                  gcCAAGGCCGCCGGCAAACGCCTGGTGATCGCCACGGCCACCGTGGCCCTGCAGGAACAGATCGTCTTCAAGGACCTGCCCGACCTGATGCGCAGCAGCGGCCTCAACTTCAGCTTCGCCCTGGCCAAGGGCCGTGGCCGCTATCTGTGc     <  2:3775331/150‑1 (MQ=255)
                                                                                                                                                    cAAGGCCGCCGGCAAACGCCTGGTGATCGCCACGGCCACCGTGGCCCTGCAGGAACAGATCGTCTTCAAGGACCTGCCCGACCTGATGCGCAGCAGCGGCCTCAACTTCAGCTTCGCCCTGGCCAAGGGCCGTGGCCGCTATCTGTGCCt   >  1:6589217/1‑150 (MQ=255)
                                                                                                                                                    cAAGGCCGCCGGCAAACGCCTGGTGATCGCCACGGCCACCGTGGCCCTGCAGGAACAGATCGTCTTCAAGGACCTGCCCGACCTGATGCGCAGCAGCGGCCTCAACTTCAGCTTCGCCCTGGCCAAGGGCCGTGGCCGCTATCTGTGCCt   <  2:6154532/150‑1 (MQ=255)
                                                                                                                                                    cAAGGCCGCCGGCAAACGCCTGGTGATCGCCACGGCCACCGTGGCCCTGCAGGAACAGATCGTCTTCAAGGACCTGCCCGACCTGATGCGCAGCAGCGGCCTCAACTTCAGCTTCGCCCTGGCCAAGGGCCGTGGCCGCTATCTGTGCCt   >  2:2217623/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TGATGATCGCCGAAGTGGCCAAGGTGCTTGGCGACATTGCCTGCGACGATGAAGGCCGCCGCGCTGGCGAGCCTGCCGTGGTGGCCGTGGAAGCGGGCACCGGTACCGGCAAGACGGTAGCCTACAGCCTGGCTGCCATCCCGGCGGCCAAGGCCGCCGGCAAACGCCTGGTGATCGCCACGGCCACCGTGGCCCTGCAGGAACAGATCGTCTTCAAGGACCTGCCCGACCTGATGCGCAGCAGCGGCCTCAACTTCAGCTTCGCCCTGGCCAAGGGCCGTGGCCGCTATCTGTGCCTG  >  NC_002947/1286715‑1287013

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑