Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,344,106 G→T 5.3% P13T (CCT→ACT)  PP_1168 ← TRAP dicarboxylate transporter subunit DctQ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,344,1060GT 5.3% 760.6 / 20.8 225P13T (CCT→ACT) PP_1168TRAP dicarboxylate transporter subunit DctQ
Reads supporting (aligned to +/- strand):  ref base G (118/95);  new base T (5/7);  total (123/102)
Fisher's exact test for biased strand distribution p-value = 3.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

Only 100 of 236 total aligned reads displayed.

GGTGCCCAGCACGTAGCGGGCGAAGATGCCCCACGGGATGATCAGCGTCATGGCCAGGATCGACAGGCCCGCGATCCAGATGCAACTGCGGTACAGCGTGTCGTTCACGCTCAGGAAAAGCGATTTCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGCTTCATCAGGTCGGCGTACTGCGCGCCGTATTTTTCGCGTACCGAAGCCGTGGCGTCGTAGAACGGCTTCTTGTCGACGGTAA  >  NC_002947/1343957‑1344255
                                                                                                                                                     |                                                                                                                                                     
ggggCCCAGCACGTAGCGGGCGAAGATGCCCCACGGGATGATCAGCGTCATGGCCAGGATCGACAGGCCCGCGATCCAGATGCAACTGCGGTACAGCGTGTCGTTCACGCTCAGGAAAAGCGATTTCATAGGCATCACCAAAGCGGCAgg                                                                                                                                                       <  1:7814771/147‑1 (MQ=255)
     ccAGCACGTAGCGGGCGAAGATGCCCCACGGGATGATCAGCGTCATGGCCAGGATCGACAGGCCCGCGATCCAGATGCAACTGCGGTACAGCGTGTCGTTCACGCTCAGGAAAAGCGATTTCATAGGCATCACCAAAGCGGCAGggcggc                                                                                                                                                  <  1:5872279/150‑1 (MQ=255)
      cAGCACGTAGCGGGCGAAGATGCCCCACGGGATGATCAGCGTCATGGCCAGGATCGACAGGCCCGCGATCCAGATGCAACTGCGGTACAGCGTGTCGTTCACGCTCAGGAAAAGCGATTTCATAGGCATCACCAAAGCGGCAGGgcggcg                                                                                                                                                 >  1:1286827/1‑150 (MQ=255)
      cAGCACGTAGCGGGCGAAGATGCCCCACGGGATGATCAGCGTCATGGCCAGGATCGACAGGCCCGCGATCCAGATGCAACTGCGGTACAGCGTGTCGTTCACGCTCAGGAAAAGCGATTTCATAGGCATCACCAAAGCGGCAGGgcggcg                                                                                                                                                 <  2:10622/150‑1 (MQ=255)
      cAGCACGTAGCGGGCGAAGATGCCCCACGGGATGATCAGCGTCATGGCCAGGATCGACAGGCCCGCGATCCAGATGCAACTGCGGTACAGCGTGTCGTTCACGCTCAGGAAAAGCGATTTCATAGGCATCACCAAAGCGGCAGGgcggcg                                                                                                                                                 >  1:8540694/1‑150 (MQ=255)
      cAGCACGTAGCGGGCGAAGATGCCCCACGGGATGATCAGCGTCATGGCCAGGATCGACAGGCCCGCGATCCAGATGCAACTGCGGTACAGCGTGTCGTTCACGCTCAGGAAAAGCGATTTCATAGGCATCACCAAAGCGGCAGGgcggcg                                                                                                                                                 >  1:4082502/1‑150 (MQ=255)
                 gggCGAAGATGCCCCACGGGATGATCAGCGTCATGGCCAGGATCGACAGGCCCGCGATCCAGATGCAACTGCGGTACAGCGTGTCGTTCACGCTCAGGAAAAGCGATTTCATAGGCATCACCAAAGCGGCAGTGCGGCGCCCGCCGCCCt                                                                                                                                      <  1:625321/150‑1 (MQ=255)
                  ggCGAAGATGCCCCACGGGATGATCAGCGTCATGGCCAGGATCGACAGGCCCGCGATCCAGATGCAACTGCGGTACAGCGTGTCGTTCACGCTCAGGAAAAGCGATTTCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTg                                                                                                                                     >  1:6837880/1‑150 (MQ=255)
                   gCGAAGATGCCCCACGGGATGATCAGCGTCATGGCCAGGATCGACAGGCCCGCGATCCAGATGCAACTGCGGTACAGCGTGTCGTTCACGCTCAGGAAAAGCGATTTCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGc                                                                                                                                    >  1:5603342/1‑150 (MQ=255)
                    cGAAGATGCCCCACGGGATGATCAGCGTCATGGCCAGGATCGACAGGCCCGCGATCCAGATGCAACTGCGGTACAGCGTGTCGTTCACGCTCAGGAAAAGCGATTTCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGcc                                                                                                                                   <  2:1369593/150‑1 (MQ=255)
                     gAAGATGCCCCACGGGATGATCAGCGTCATGGCCAGGATCGACAGGCCCGCGATCCAGATGCAACTGCGGTACAGCGTGTCGTTCACGCTCAGGAAAAGCGATTTCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCg                                                                                                                                  >  1:7121691/1‑150 (MQ=255)
                     gAAGATGCCCCACGGGATGATCAGCGTCATGGCCAGGATCGACAGGCCCGCGATCCAGATGCAACTGCGGTACAGCGTGTCGTTCACGCTCAGGAAAAGCGATTTCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCg                                                                                                                                  >  1:3556226/1‑150 (MQ=255)
                      aaGATGCCCCACGGGATGATCAGCGTCATGGCCAGGATCGACAGGCCCGCGATCCAGATGCAACTGCGGTACAGCGTGTCGTTCACGCTCAGGAAAAGCGATTTCATAGGCATCACCAAAGCGGCAGTGCGGCGCCCGCCGCCCTGCCgc                                                                                                                                 <  2:5396150/150‑2 (MQ=255)
                      aaGATGCCCCACGGGATGATCAGCGTCATGGCCAGGATCGACAGGCCCGCGATCCAGATGCAACTGCGGTACAGCGTGTCGTTCACGCTCAGGAAAAGCGATTTCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGa                                                                                                                                 <  1:3174908/150‑1 (MQ=255)
                      aaGATGCCCCACGGGATGATCAGCGTCATGGCCAGGATCGACAGGCCCGCGATCCAGATGCAACTGCGGTACAGCGTGTCGTTCACGCTCAGGAAAAGCGATTTCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGa                                                                                                                                 <  2:4198615/150‑1 (MQ=255)
                           gCCCCACGGGATGATCAGCGTCATGGCCAGGATCGACAGGCCCGCGATCCAGATGCAACTGCGGTACAGCGTGTCGTTCATGCTCAGGAAAAGCGATTTCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCg                                                                                                                            >  1:2361093/1‑150 (MQ=255)
                             cccACGGGATGATCAGCGTCATGGCCAGGATCAACAGGCCCGCGATCCAGATGCAACTGCGGTACAGCGTGTCGTTCACGCTCAGGAAAAGCGATTTCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGAt                                                                                                                          >  2:5598700/1‑150 (MQ=255)
                              ccACGGGATTATCAGCGTCATGGCCAGGATCGACAGGCCCGCGATCCAGATGAAACTGCGGTACAGCGTGTCGTTCACGCTCAGTAAAAGCGATTTAATACACATCACAAAACCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGAtt                                                                                                                         <  2:3193638/150‑1 (MQ=255)
                              ccACGGGATGATCAGCGTCATGGCCAGGATCGACAGGCCCGCGATCCAGATGCAACTGCGGTACAGCGTGTCGTTCACGCTCAGGAAAAGCGATTTCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGGtt                                                                                                                         >  1:8898376/1‑150 (MQ=255)
                              ccACGGGATGATCAGCGTCATGGCCAGGATCGACAGGCCCGCGATCCAGATGCAACTGCGGTACAGCGTGTCGTTCACGCTCAGGAAAAGCGATTTCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGAtt                                                                                                                         <  2:5886612/150‑1 (MQ=255)
                              ccACGGGATGATCAGCGTCATGGCCAGGATCGACAGGCCCGCGATCCAGATGCAACTGCGGTACAGCGTGTCGTTCACGCTCAGGAAAAGCGATTTCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGAtt                                                                                                                         >  2:4038452/1‑150 (MQ=255)
                              ccACGGGATGATCAGCGTCATGGCCAGGATCGACAGGCCCGCGATCCAGATGCAACTGCGGTACAGCGTGTCGTTCACGCTCAGGAAAAGCGATTTCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGAtt                                                                                                                         >  1:7552644/1‑150 (MQ=255)
                               cACGGGATGATCAGCGTCATGGCCAGGATCGACAGGCCCGCGATCCAGATGCAACTGCGGTACAGCGTGTCGTTCACGCTCAGGAAAAGCGATTTCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTg                                                                                                                        <  2:2565764/150‑1 (MQ=255)
                                    gatgatCAGCGTCATGGCCAGGATCGACAGGCCCGCGATCCAGATGCAACTGCGGTACAGCGTGTCGTTCACGCTCAGGAAAAGCGATTTCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCaa                                                                                                                   >  1:1449142/1‑150 (MQ=255)
                                      tgatCAGCGTCATGGCCAGGATCGACAGGCCCGCGAACCAGATGCAACTGCGGTACAGCGTGACGTTCACGCTCAGGAAAAGCTATATCATAGTCATCACCAAATCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAgg                                                                                                                 >  1:6921717/1‑150 (MQ=255)
                                           aGCGTCATGGCCAGGATCGACAGGCCCGCGATCCAGATGCAACTGCGTTACAGCGTCTCGTTCACGCTCAGCAAAACCGATTTCATAGGCAACACCAAAGCGGCAGGGCGGCGCGCGCCCCACTGCCGAGGTCGATTGGGCAAGggcggc                                                                                                            <  2:6722182/150‑1 (MQ=255)
                                             cGTCATGGCCAGGATCGACAGGCCCGCGATCCAGATGCAACTGCGGTACAGCGTGTCGTTCACGCTCAGGAAAAGCGATTTCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTg                                                                                                          <  2:3954547/150‑1 (MQ=255)
                                               tCATGGCCAGGATCGACAGGCCCGCGATCCAGATGCAACTGCGGTACAGCGTGTCGTTCACGCTCAGGAAAAGCGATTTCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGtt                                                                                                        >  2:7847846/1‑150 (MQ=255)
                                                cATGGCCTGCAGCGACCGGCCCGCGATCAAGATGCAACTGCGGTACCGCGTGTCGTACCCGCGCAGCAAAAGCGATTTCATAGGAATCACCAACGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTa                                                                                                       <  1:832119/150‑1 (MQ=255)
                                                cATGGCCAGGATCGACAGGCCCGCGATCCAGATGCAACTGCGGTACAGCGTGTCGTTCACGCTCAGGAAAAGCGATTTCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTa                                                                                                       <  1:3596762/150‑1 (MQ=255)
                                                  tGGCCTGGATCGACAGGCCCGCTTTCCTTTTTCAACTGGGGTTCAGCGTGTATTTCAGGCTCAGGAAAAGCGTTTTCATAGGCATCACCTAAGGTGCAGGGCTGGTGGCGCCGCAATGCCGAGGTCGATTTGGCAAGGGCGGCTGTTACt                                                                                                     <  2:8849963/150‑1 (MQ=255)
                                                  tGGCCAGGATCGACAGGCCCGCGATCCAGATGCAACTGCGGTACAGCGTGTCGTTCACGCTCAGGAAAAGCGATTTCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACt                                                                                                     <  2:7312357/150‑1 (MQ=255)
                                                  gggCCAGGATCGACAGGCCGGCGAACCAGATCCAACTGCGGTACCGCGTGCCGTTCCCGCACAGGAAAAGCGATTCCATCGGCAACACCAACGCGGCAGGGCGGCGGGCGCCGCACGGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACt                                                                                                     <  1:5411598/149‑1 (MQ=255)
                                                   ggCCAGGATCGACAGGCCCGCGATCCAGATGCAACTGCGGTACAGCGTGTCGTTCACGCTCAGGAAAAGCGATTTCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTg                                                                                                    <  1:2855152/150‑1 (MQ=255)
                                                     ccAGGATCGACAGGCCCGCGATCCAGATGCAACTGCGGTACAGCGTGTCGTTCACGCTCAGGAAAAGCGATTTCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGa                                                                                                  <  2:2614391/150‑1 (MQ=255)
                                                      cAGGATCGACAGGCCCGCGATCCAGATGCAACTGCGGTACAGCGTGTCGTTCACGCTCAGTAAAAGCGATTTCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGAc                                                                                                 <  2:1195039/150‑1 (MQ=255)
                                                      cAGGATCGACAGGCCCACGATCCAGATGCAACTGCGGTACAGCGTGTCGTTCACGCTCAGGAAAAGCGATTTCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGAc                                                                                                 >  2:1311585/1‑150 (MQ=255)
                                                       tggATCGACCGGCCCGCGATCCAGATGCAACTGGGGTACTGCGTGTGGTTCCCGCTCCGGAAAAGCGATTGCATCGGCATCACCAACGCGGCCGGGGGGGGGGCGCGGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACg                                                                                                <  1:2622540/149‑1 (MQ=255)
                                                         gATCGACAGGCCCGCGATCCAGATGCAACTGCGGTACAGCGTGTCGTTCACGCTCAGGAAAAGCGATTTCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGc                                                                                              >  2:3631670/1‑150 (MQ=255)
                                                          aTCGACAGGCCCGCGATCCAGATGCAACTGCGGTACAGCGTGTCGTTCACGCTCAGGAAAAGCGATTTCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCg                                                                                             >  1:5684770/1‑150 (MQ=255)
                                                           tCGACTGGCCCGCTATCCTTTTTAAACTGCGGTACTGCTTGTCGTACACGCTCAGGAAAAGCTATTTAATAGGAATCACAAAAGGGGCAGGGCGGGTGGCGCCGAACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGt                                                                                            <  1:4822990/150‑1 (MQ=255)
                                                           tCGACCGGCCCGCGATCCAGATGCAACTGCGGTACAGCGTGTCGTTCACGCTCAGGAAAAGCGATTTCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGATGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGt                                                                                            <  1:1411190/150‑1 (MQ=255)
                                                             gACAGGCCCGCGATCCAGATGCAACTGCGGTACAGCGTGTCGTTCACGCTCAGGAAAAGCGATTTCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCg                                                                                          >  2:1308863/1‑150 (MQ=255)
                                                               cAGGCCCGCGATCCAGATGCAACGGCGGTACAGCGTGTCGTTCACGCTCCGGAAAAGCGATTTCATAGGCATCCCCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGAt                                                                                        <  1:3464399/150‑1 (MQ=255)
                                                                 ggCCCGCGATCCAGATGCAACTGCGGTACAGCGTGTCGTTCACGCTCAGGAAAAGCGATTTCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATAc                                                                                      <  2:5076848/150‑1 (MQ=255)
                                                                    ccGCGATCCAGATGCAACTGCGGTACAGCGTGTCGTTCACGCTCAGGAAAAGCGATTTCATAGGCATCACCAATGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGCt                                                                                   >  2:8003180/1‑150 (MQ=255)
                                                                      gccacccAGATCCAACTGCGCAACAGCGTTTCGTTCACGCACAGCAAAAGCGATTACATAGCAATCACCAAACCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGCTtc                                                                                 <  1:84479/145‑1 (MQ=255)
                                                                       ctaTCCAGATCCAACTGCGGTACAGCGTCTCTTACACGCTCAGCAAAAGCGATTTCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGCTtca                                                                                <  2:6116135/148‑1 (MQ=255)
                                                                            cAGATGCAACTGCGGTACAGCGTGTCGTTCACGCTCAGGAAAAGCGATTTCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGCTTCATCAgg                                                                           >  2:2667982/1‑150 (MQ=255)
                                                                            cAGATGCAACTGCGGTACAGCGTGTCGTTCACGCTCAGGAAAAGCGATTTCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGCTTCATCAgg                                                                           >  2:6780318/1‑150 (MQ=255)
                                                                             aGATGCAACTGCGGTACAGCGTGTCGTTCACGCTCAGGAAAAGCGATTTCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGCTTCATCAGGt                                                                          >  2:4619396/1‑150 (MQ=255)
                                                                             aGATGCAACTGCGGTACAGCGTGTCGTTCACGCTCAGGAAAAGCGATTACATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGCTTCATCAGGt                                                                          <  1:5210735/150‑1 (MQ=255)
                                                                              gATGCAACTGCGGTACAGCGTGTCGTTCACGCTCAGGAAAAGCGATTTCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGCTTCATCAGGTc                                                                         >  1:4377388/1‑150 (MQ=255)
                                                                                   aaCTGCGGTACAGCGTGTCGTTCACGCTCAGGAAAAGCGATTTCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGCTTCATCAGGTCGGCGt                                                                    <  1:8397560/150‑1 (MQ=255)
                                                                                     cTGCGGTACAGCGTGTCGTTCACGCTCAGGAAAAGCGATTTCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGCTTCATCAGGTCGGCGTAc                                                                  <  2:6197290/150‑1 (MQ=255)
                                                                                      tGCGGTACAGCGTGTCGTTCACGCTCAGGAAAAGCGATTTCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGCTTCATCAGGTCGGCGTACt                                                                 >  2:3805669/1‑150 (MQ=255)
                                                                                             cAGCGTGTCGTTCACGCTCAGGAAAAGCGATTTCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGCTTCATCAGGTCGGCGTACTGCGCGcc                                                          >  1:7372240/1‑150 (MQ=255)
                                                                                               gCGTGTCGTTCACGCTCAGGAAAAGCGATTTCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGCTTCATCAGGTCGGCGTACTGCGCGCCGt                                                        >  1:4519436/1‑150 (MQ=255)
                                                                                                cGTGTCGTTCACGCTCAGGAAAAGCGATTTCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGCTTCATCAGGTCGGCGTACTGCGCGCCGTa                                                       >  2:7439857/1‑150 (MQ=255)
                                                                                                 gtgtCGTTCACGCTCAGGAAAAGCGATTTCATAGGCATCACCAAAGCGGCTGGGCGGGGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGCTTCATCAGGTCGGCGTACTGCGCGCCGTAt                                                      <  1:782598/150‑1 (MQ=255)
                                                                                                  tgtCGTTCACGCTCAGGAAAAGCGATTTCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGCTTCATCAGGTCGGCGTACTGCGCGCCGTAtt                                                     >  2:1454021/1‑150 (MQ=255)
                                                                                                   gtCGTTCACGCTCAGGAAAAGCGATTTCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGCTTCATCAGGTCGGCGTACTGCGCGCCGTAttt                                                    <  2:7121691/150‑1 (MQ=255)
                                                                                                    tCGTTCACGCTCAGGAAAAGCGATTTCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGCTTCATCAGGTCGGCGTACTGCGCGCCGTAtttt                                                   >  2:2281264/1‑150 (MQ=255)
                                                                                                      tttCACGATCAGTAAATGCTATTTAATAGGCATCACCAAAGCGGATGGGGGGGGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGCTTCATCAGGTCGGCGTACTGCGCGCCGTATTTTTc                                                 <  1:4194835/149‑1 (MQ=255)
                                                                                                      gTTCACGCTCAGGAAAAGCGATTTCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGCTTCATCAGGTCGGCGTACTGCGCGCCGTATTTTTc                                                 <  2:4864399/150‑1 (MQ=255)
                                                                                                          aCGCTCAGGAAAAGCGATTTCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGCTTCATCAGGTCGGCGTACTGCGCGCCGTATTTTTCGCGt                                             >  2:8360218/1‑150 (MQ=255)
                                                                                                                aGGAAAAGCGATTTCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCt                                                                                                           <  2:822579/82‑1 (MQ=255)
                                                                                                                aGGAAAAGCGATTTCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCt                                                                                                           >  1:822579/1‑82 (MQ=255)
                                                                                                                aGGAAAAGCGATTTCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGCTTCATCAGGTCGGCGTACTGCGCGCCGTATTTTTCGCGTACCGaa                                       >  2:1484083/1‑150 (MQ=255)
                                                                                                                  gAAAAGCGATTTCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGCTTCATCAGGTCGGCGTACTGCGCGCCGTATTTTTCGCGTACCGAAGc                                     >  2:2743686/1‑150 (MQ=255)
                                                                                                                  gAAAAGCGATTTCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGCTTCATCAGGTCGGCGTACTGCGCGCCGTATTTTTCGCGTACCGAAGc                                     >  1:2803222/1‑150 (MQ=255)
                                                                                                                  gAAAAGCGATTTCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGCTTCATCAGGTCGGCGTACTGCGCGCCGTATTTTTCGCGTACCGAAGc                                     >  2:9150445/1‑150 (MQ=255)
                                                                                                                   aaaaGCGATTTCATCGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGCTTCATCAGGTCGGCGTACTGCGCGCCGTATTTTTCGCGTACCGAAGcc                                    <  1:489354/150‑1 (MQ=255)
                                                                                                                   aaaaGCGATTTCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGCTTCATCAGGTCGGCGTACTGCGCGCCGTATTTTTCGCGTACCGAAGcc                                    >  1:1114359/1‑150 (MQ=255)
                                                                                                                      aGCGATTTCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGCTTCATCAGGTCGGCGTACTGCGCGCCGTATTTTTCGCGTACCGAAGCCGTg                                 <  2:406461/150‑1 (MQ=255)
                                                                                                                       gCGATTTCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGCTTCATCAGGTCGGCGTACTGCGCGCCGTATTTTTCGCGTACCGAAGCCGTgg                                >  1:8006252/1‑150 (MQ=255)
                                                                                                                       gCGATTTCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGCTTCATCAGGTCGGCGTACTGCGCGCCGTATTTTTCGCGTACCGAAGCCGTgg                                >  1:6820953/1‑150 (MQ=255)
                                                                                                                         gATTTCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGCTTCATCAGGTCGGCGTACTGCGCGCCGTATTTTTCGCGTACCGAAGCCGTGGcg                              >  2:6455449/1‑150 (MQ=255)
                                                                                                                           tttCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGCTTCATCAGGTCGGCGTACTGCGCGCCGTATTTTTCGCGTACCGAAGCCGTGGcgtc                            >  2:2913411/1‑150 (MQ=255)
                                                                                                                               aTAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGCTTCATCAGGTCGGCGTACTGCGCGCCGTATTTTTCGCGTACCGAAGCCGTGGCGTCGTAg                        >  1:6661205/1‑150 (MQ=255)
                                                                                                                                  ggCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGCTTCATCAGGTCGGCGTACTGCGCGCCGTATTTTTcg                                                >  2:9117919/1‑123 (MQ=255)
                                                                                                                                  ggCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGCTTCATCAGGTCGGCGTACTGCGCGCCGTATTTTTcg                                                <  1:9117919/123‑1 (MQ=255)
                                                                                                                                   gCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGCTTCATCAGGTCGGCGTACTGCGCGCCGTATTTTTCGCGTACCGAAGCCGTGGCGTCGTAGAACg                    >  2:581773/1‑150 (MQ=255)
                                                                                                                                   gCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGCTTCATCAGGTCGGCGTACTGCGCGCCGTATTTTTCGCGTACCGAAGCCGTGGCGTCGTAGAACg                    >  1:2719714/1‑150 (MQ=255)
                                                                                                                                   gCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGCTTCATCAGGTCGGCGTACTGCGCGCCGTATTTTTCGCGTACCGAAGCCGTGGCGTCGTAGAACg                    >  1:7070427/1‑150 (MQ=255)
                                                                                                                                     aTCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGCTTCATCAGGTCGGCGTACTGCGCGCCGTATTTTTCGCGTACCGAAGCCGTGGCGTCGTAGAACGGc                  <  2:2175670/150‑1 (MQ=255)
                                                                                                                                     aTCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGCTTCATCAGGTCGGCGTACTGCGCGCCGTATTTTTCGCGTACCGAAGCCGTGGCGTCGTAGAACGGc                  >  2:1685530/1‑150 (MQ=255)
                                                                                                                                        aCCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGCTTCATCAGGTCGGCGTACTGCGCGCCGTATTTTTCGCGTACCGAAGCCGTGGCGTCGTAGAACGGcttc               >  1:2020709/1‑150 (MQ=255)
                                                                                                                                         ccAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGCTTCATCAGGTCGGCGTACTGCGCGCCGTATTTTTCGCGTACCGAAGCCGTGGCGTCGTAGAACGGcttct              >  2:575573/1‑150 (MQ=255)
                                                                                                                                           aaaGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGCTTCATCAGGTCGGCGTACTGCGCGCCGTATTTTTCGCGTACCGAAGCCGTGGCGTCGTAGAACGGCTTCTTg            >  1:1831452/1‑150 (MQ=255)
                                                                                                                                            cctcGGCAGTGCGGCGCCCGCCGCCCTGCCGCGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGCTTCATCAGGTCGGCGTACTGCGCGCCGTATTTTTCGCGTACCGAAGCCGTGGCGTCGTAGAACGGCTTCTTGt           >  1:5907129/4‑150 (MQ=255)
                                                                                                                                            aaGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGCTTCATCAGGTCGGCGTACTGCGCGCCGTATTTTTCGCGTACCGAAGCCGTGGCGTCGTAGAACGGCTTCTTGt           <  1:1312354/150‑1 (MQ=255)
                                                                                                                                             aGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGCTTCATCAGGTCGGCGTACTGCGCGCCGTATTTTTCGCGTACCGAAGCCGTGGCGTCGTAGAACGGCTTCTTGTc          <  2:8540694/150‑1 (MQ=255)
                                                                                                                                             aGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGCTTCATCAGGTCGGCGTACTGCGCGCCGTATTTTTCGCGTACCGAAGCCGTGGCGTCGTAGAACGGCTTCTTGTc          <  2:3434963/150‑1 (MQ=255)
                                                                                                                                             aGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGCTTCATCAGGTCGGCGTACTGCGCGCCGTATTTTTCGCGTACCGAAGCCGTGGCGTCGTAGAACGGCTTCTTGTc          <  2:4132187/150‑1 (MQ=255)
                                                                                                                                                ggCAGTGCGGCGCCCGCCGCCCTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGCTTCATCAGGTCGGCGTACTGCGCGCCGTATTTTTCGCGTACCGAAGCCGTGGCGTCGTAGAACGGCTTCTTGTCGAc       >  1:5277299/1‑150 (MQ=255)
                                                                                                                                                ggCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGCTTCATCAGGTCGGCGTACTGCGCGCCGTATTTTTCGCGTACCGAAGCCGTGGCGTCGTAGAACGGCTTCTTGTCGAc       >  1:328816/1‑150 (MQ=255)
                                                                                                                                                 gCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGCTTCATCAGGTCGGCGTACTGCGCGCCGTATTTTTCGCGTACCGAAGCCGTGGCGTCGTAGAACGGCTTCTTGTCGACg      >  2:1252202/1‑150 (MQ=255)
                                                                                                                                                  cAGTGCGGCGCCCGCCGCCCTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGCTTCATCAGGTCGGCGTACTGCGCGCCGTATTTTTCGCGTACCGAAGCCGTGGCGTCGTAGAACGGCTTCTTGTCGACgg     >  2:4460847/1‑150 (MQ=255)
                                                                                                                                                    gggCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGCTTCATCAGGTCGGCGTACTGCGCGCCGTATTTTTCGCGTACCGAAGCCGTGGCGTCGTAGAACGGCTTCTTGTCGACGGTa   >  1:7240404/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GGTGCCCAGCACGTAGCGGGCGAAGATGCCCCACGGGATGATCAGCGTCATGGCCAGGATCGACAGGCCCGCGATCCAGATGCAACTGCGGTACAGCGTGTCGTTCACGCTCAGGAAAAGCGATTTCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGCTTCATCAGGTCGGCGTACTGCGCGCCGTATTTTTCGCGTACCGAAGCCGTGGCGTCGTAGAACGGCTTCTTGTCGACGGTAA  >  NC_002947/1343957‑1344255

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑