BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,383,260	A→T	9.7%	L16Q (CTG→CAG)	PP_1204 ←	lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,383,260	0	A	T	9.7%	675.1 / 38.3	288	L16Q (CTG→CAG)	PP_1204	lipoprotein
Reads supporting (aligned to +/- strand): ref base A (114/122); new base T (6/26); total (132/156)
Fisher's exact test for biased strand distribution p-value = 2.03e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 295 total aligned reads displayed.

GCACACGTCGCTGCGGAAGCTCCATCACCTTCGGCTCGCCGACATCGATCTGCAGGGTATGCTCGAGCAGTTTGCGCTCGACGCGCTCCTCATGGTCGCTGCGCTGGGACATCTGGTTGGCGCAGCCGCTGACGAGCAGGGCGCCGCACAGTGCGGCGCCCCCAAGGGTAAAGGTACTTCGCTTGTACATGATCACTCGTGCATTGGTTATCAGCGGCGAACGCGGGCCTGCAGGAAGGTCAGCACTTCATTGAGCGGCAACGCCTGGGCGTCCTGCTCGGTGCGGTGCTTGTACTCCA  >  NC_002947/1383111‑1383409

gcACACGTCGCTGCGGAAGCTCCATCACCTTCGGCTCGCCGACATCGATCTGCAGGGTATGCTCGAGCAGTTTGCGCTCGACGCGCTCCTCATGGTCGCTGCGCTGGGACATCTGGTTGGCGCAGCCGCTGACGAGCAGGGCGCCGccca                                                                                                                                                       >  1:6545294/1‑150 (MQ=255)

gcACACGTCGCTGCGGAAGCTCCATCACCTTCGGCTCGCCGACATCGATCTGCAGGGTATGCTCGAGCAGTTTGCGCTCGACGCGCTCCTCATGGTCGCTGCGCTGGGACATCTGGTTGGCGCAGCCGCTGACGAGCAGGGCGCCCcaca                                                                                                                                                       >  1:1932800/1‑150 (MQ=255)

  acacGTCGCTGCGGAAGCTCCATCACCTTCGGCTCGCCGACATCGATCTGCAGGGTATGCTCGAGCAGTTTGCGCTCGACGCGCTCCTCATGGTCGCTGCGCTGGGACATCTGGTTGGCGCAGCCGCTGACGAGCAGGGCGCCGCACTGt                                                                                                                                                     <  1:6921890/150‑1 (MQ=255)

   cacGTCGCTGCGGAAGCTCCATCACCTTCGGCTCGCCGACATCGATCTGCAGGGTATGCTCGAGCAGTTTGCGCTCGACGCGCTCCTCATGGTCGCTGCGCTGGGACATCTGGTTGGCGCAGCCGCTGACGAGCAGGGCGCCGCACAGTg                                                                                                                                                    <  2:911418/150‑1 (MQ=255)

   cacGTCGCTGCGGAAGCTCCATCACCTTCGGCTCGCCGACATCGATCTGCAGGGTATGCTCGAGCAGTTTGCGCTCGACGCGCTCCTCATGGTCGCTGCGCTGGGACATCTGGTTGGCGCAGCCGCTGACGAGCAGGGCGCCGCACAGTg                                                                                                                                                    <  1:2458970/150‑1 (MQ=255)

    acGTCGCTGCGGAAGCTCCATCACCTTCGGCTCGCCGACATCGATCTGCAGGGTATGCTCTAGCAGTTTGCGCTCGACGCGCTCCTAATGGTCGCTGCGCTGGGACATCTGGTTGGCGCAGCCGCTGACGAGCAGGGCGCCGCACAGTgc                                                                                                                                                   <  2:5983907/150‑1 (MQ=255)

    acGTCGCTGCGGAAGCTCCATCACCTTCGGCTCGCCGACATCGATCTGCAGGGTATGCTCGAGCAGTTTGCGCTCGACGCGCTCCTCATGGTCGCTGCGCTGGGACGTCTGGTTGGCGCAGCCGCTGACGAGCAGGGCGCCGCACAGTgc                                                                                                                                                   <  1:8007042/150‑1 (MQ=255)

     cGTCGCTGCGGAAGCTCCATCACCTTCGGCTCGCCGACATCGATCTGCAGGGTATGCTCGAGCAGTTTGCGCTCGACGCGCTCCTCATGGTCGCTGCGCTGGGACATCTGGTTGGCGCAGCCGCTGACGAGCAGGGCGCCGCACTGTgcg                                                                                                                                                  <  1:590162/150‑1 (MQ=255)

     cGTCGCTGCGGAAGCTCCATCACCTTCGGCTCGCCGACATCGATCTGCAGGGTATGCTCGAGCAGTTTGCGCTCGACGCGCTCCTCATGGTCGCTGCGCTGGGACATCTGGTTGGCGCAGCCGCTGACGAGCAGGGCGCCGCACAGTgcg                                                                                                                                                  <  2:3700622/150‑1 (MQ=255)

          cTGCGGAAGCTCCATCACCTTCGGCTCGCCGACATCGATCTGCAGGGTATGCTCGAGCAGTTTGCGCTCGACGCGCTCCTCATGGTCGCTGCGCTGGGACATCTGGTTGGCGCAGCCGCTGACGAGCAGGGCGCCGCACTGTGCGGCGcc                                                                                                                                             <  2:8901029/150‑1 (MQ=255)

           tGCGGAAGCTCCATCACCTTCGGCTCGCCGACATCGATCTGCAGGGTATGCTCGAGCAGTTTGCGCTCGACGCGCTCCTCATGGTCGCTGCGCTGGGACATCTGGTTGGCGCAGCCGCTGACGAGCAGGGCGCCGCACAGTGCGGCGccc                                                                                                                                            >  2:1348745/1‑150 (MQ=255)

               gAAGCTCCATCACCTTCGGCTCGCCGACATCGATCTGCAGGGTATGCTCGAGCAGTTTGCGCTCGACGCGCTCCTCATGGTCGCTGCGCTGGGACATCTGGTTGGCGCAGCCGCTGACGAGCAGGGCGCCGCACAGTGCGGCGCCCCCaa                                                                                                                                        >  2:2569218/1‑150 (MQ=255)

                 aGCTCCATCACCTTCGGCTCGCCGACATCGATCTGCAGGGTATGCTCGAGCAGTTTGCGCTCGACGCGCTCCTCATGGTCGCTGCGCTGGGACATCTGTTTGGCGCAGCCGCTGACGAGCAGGGCGCCGCACAGTGCGGCGCCCCCAAgg                                                                                                                                      <  1:5568590/150‑1 (MQ=255)

                 aGCTCCATCACCTTCGGCTCGCCGACATCGATCTGCAGGGTATGCTCGAGCAGTTTGCGCTCGACGCGCTCCTCATGGTCGCTGCGCTGGGACATCTGGTTGGCGCAGCCGCTGACGAGCAGGGCGCCGCACAGTGCGGCGCCCCCAAgg                                                                                                                                      <  2:2701760/150‑1 (MQ=255)

                 aGCTCCATCACCTTCGGCTCGCCGACATCGATCTGCAGGGTATGCTCGAGCAGTTTGCGCTCGACGCGCTCCTCATGGTCGCTGCGCTGGGACATCTGGTTGGCGCAGCCGCTGACGAGCAGGGCGCCGCACAGTGCGGCGCCCCCAAgg                                                                                                                                      <  2:1443363/150‑1 (MQ=255)

                    tCCATCACCTTCGGCTCGCCGACATCGATCTGCAGGGTATGCTCGAGCAGTTTGCGCTCGACGCGCTCCTCATGGTCGCTGCGCTGGGACATCTGGTTGGCGCAGCCGCTGACGAGCAGGGCGCCGCACAGTGCGGCGCCCCCAAGGGTa                                                                                                                                   >  2:440609/1‑150 (MQ=255)

                    tCCATCACCTTCGGCTCGCCGACATCGATCTGCAGGGTATGCTCGAGCAGTTTGCGCTCGACGCGCTCCTCATGGTCGCTGCGCTGGGACATCTGGTTGGCGCAGCCGCTGACGAGCAGGGCGCCCCCCAGAGCCGCGcccccccgggga                                                                                                                                   >  1:779543/1‑143 (MQ=255)

                      cATCACCTTCGGCTCGCCGACTTCGATCTGCTGGGTATGCTCGAGCTGTTTGCGCTCGACGGGCTCCTCTTGGTCGCTGCGCTGGGACATCTGGTTGGCGCAGCCGCTGACGAGCTGGGCGCCGCACAGTGCGGCGCCCCCAAGGGTaaa                                                                                                                                 <  1:6687042/150‑1 (MQ=255)

                       aTCACCTTCGGCTCGCCGACATCGATCTGCAGGGTATGCTCGAGCAGTTTGCGCTCGACGCGCTCCTCATGGTCGCTGCGCTGGGACATCTGGTTGGCGCAGCCGCTGACGAGCAGGGCGCCGCACAGTGCGGCGCCCCCAAGGGTAAAg                                                                                                                                <  2:6089525/150‑1 (MQ=255)

                        tCACCTTCGGCTCGCCGACATCGATCTGCAGGGTATGCTCGAGCAGTTTGCGCTCGACGCGCTCCTCATGGTCGCTGCGCTGGGACATCTGGTTGGCGCAGCCGCTGACGAGCAGGGCGCCGCCCCGTGCGGCGCCCCCCAGGGTaaatg                                                                                                                               >  1:29182/1‑148 (MQ=255)

                          aCCTTCGGCTCGCCGACATCGATCTGCAGGGTTTGCTCGAGCAGTTTGCGCTCGACGCGCTCCTCATGGTCGCTGCGCTGGGACATCTGGTTGGCGCAGCCGCTGACGAGCAGGGCGCCGCACAGTGCGGCGCCCCCAAGGGTAAAGGTa                                                                                                                             <  1:7433696/150‑1 (MQ=255)

                                ggCTCGCCGACATCGATCTGCAGGGTATGCTCGAGCAGTTTGCGCTCGACGCGCTCCTCATGGTCGCTGCGCTGGGACATCTGGTTGGCGCAGCCGCTGACGAGCAGGGCGCCGCACAGTGCGgcgc                                                                                                                                              >  1:4933029/1‑127 (MQ=255)

                                ggCTCGCCGACATCGATCTGCAGGGTATGCTCGAGCAGTTTGCGCTCGACGCGCTCCTCATGGTCGCTGCGCTGGGACATCTGGTTGGCGCAGCCGCTGACGAGCAGGGCGCCGCACAGTGCGGCGCCCCCAAGGGTAAAGGTACTTCGc                                                                                                                       >  2:193958/1‑150 (MQ=255)

                                ggCTCGCCGACATCGATCTGCAGGGTATGCTCGAGCAGTTTGCGCTCGACGCGCTCCTCATGGTCGCTGCGCTGGGACATCTGGTGGGCGCAGCCGCTGACGAGCAGGGCGCCGCACAGTGCGGCGCCCCCAAGGGTAAAGGTACTTCGc                                                                                                                       <  1:8354104/150‑1 (MQ=255)

                                   tcaccGACATCGATCTGCAGGGTATGCTCGAGCAGTTTGCGCTCGACGCGCTCCTCATGGTCGCTGCGCTGGGACATCTGGTTGGCGCAGCCGCTGACGAGCAGGGCGCCGCACACTGCGGCGCCCCCAAGGGTAAAGGTACTTAGCATg                                                                                                                    >  1:1117098/4‑150 (MQ=255)

                                        gACAACGATCTGCAGGGTATGCTCGAGCAGTTTGCGCTCGACGCGCTCCTCATGGTCGCTGCGCTGGGACAACTGGTTGGCGCAGCCGCTGACGAGCAGGGCGCCGCACAGTGCGGCGCCCCCAAGCGTAAAGGTACTTCGCTTGTACAt                                                                                                               <  2:5499644/150‑1 (MQ=255)

                                           aTCGATCTGCAGGGTATGCTCGAGCAGTTTGCGCTCGACGCGCTCCTCATGGTCGCTGCGCTGGGACATCTGGTTGGCGCAGCCGCTGACGAGCAGGGCGCCGCCCAGTGCGGCGCCCCCCAGGGTAAAGGTACTTCGCGTGTACATGAt                                                                                                            >  1:437153/1‑150 (MQ=255)

                                           aTCGATCTGCAGGGTATGCTCGAGCAGTTTGCGCTCGACGCGCTCCTCATGGTCGCTGCGCTGGGACATCTGGTTGGCGCAGCCGCTGACGAGCAGGGCGCCGCACAGTGCGGCGCCCCCAAGGGTAAAGGTACTTCGCTTGTACATGAt                                                                                                            >  2:838435/1‑150 (MQ=255)

                                            tCGATCTGCAGGGTATGCTCGAGCAGTTTGCGCTCGACGCGCTCCTCATGGTCGCTGCGCTGGGACATCTGGTTGGCGCAGCCGCTGACGAGCAGGGCGCCGCACCGTGCGGCGCCCCCAAGGGGAAAGGTACTTCGCTTGTACATGATc                                                                                                           >  1:3448509/1‑150 (MQ=255)

                                             ctatgtGCTGGGTAGGCTCGAGCAGTTTGCGCTCGACGCGCTCCTATTGGTCGCTGGGCTGGGACATCTGGTTGGCGCCGCCGGTGAGGAGCTGGGCGCCGCACCGTGCGGCGCCCCCAAGGGTAAAGGTACTTCGCTTGTACATGATCa                                                                                                          <  1:1170292/145‑1 (MQ=255)

                                               aTCTGCAGGGTATGCTCGAGCAGTTTGCGCTCGACGCGCTCCTCATGGTCGCTGCGCTGGGACATCTGGTTGGCGCAGCCGCTGACGAGCAGGGCGCCGCACAGTGCGGCGCCCCCAAGGGTAAAGGTACTTCGCTTGTACATGATCACt                                                                                                        >  1:2762248/1‑150 (MQ=255)

                                                tCTGCAGGGTATGCTCGAGCAGTTTGCGCTCGACGCGCTCCTCATGGTCGCTGCGCTGGGACATCTGGTTGGCGCAGCCGCTGACGAGCAGGGCGCCGCACAGTGCGGCGCCCCCAAGGGTAAAGGTACTTCGCTTGTACATGATCACTc                                                                                                       <  2:2261782/150‑1 (MQ=255)

                                                  tGCAGGGTATGCTCGAGCAGTTTGCGCTCGACGCGCTCCTCATGGTCGCTGCGCTGGGACATCTGGTTGGCGCAGCCGCTGACGAGCAGGGCGCCGCACAGTGCGGCGCCCCCAAGGGTAAAGGTACTTCGCTTGTACATGATCACTCGt                                                                                                     >  2:3846347/1‑150 (MQ=255)

                                                  tGCAGGGTATGCTCGAGCAGTTTGCGCTCGACGCGCTCCTCATGGTCGCTGCGCTGGGACATCTGGTTGGCGCAGCCGCTGACGAGCAGGGCGCCGCACAGTGCGGCGCCCCCAAGGGGAAAGGTACTTCGCGTGTACATGATCACTCgg                                                                                                     >  1:6945143/1‑149 (MQ=255)

                                                   gCAGGGTATGCTCGAGCAGTTTGCGCTCGACGCGCTCCTCATGGTCGCTGCGCTGGGACATCTGGTTGGCGCAGCCGCTGACGAGCAGGGCGCCGCACAGTGCGGCGCCCCCAAGGGTAAAGGTACTTCGCTTGTACATGATCACTCGTg                                                                                                    >  1:2239619/1‑150 (MQ=255)

                                                   gCAGGGTATGCTCGAGCAGTTTGCGCTCGACGCGCTCCTCATGGTCGCTCCGCTGGGACATCTGGTTGGCGCAGCCGCTGACGAGCAGGGCGCCGCACAGTGCGGCGCCCCCAAGGGGAAAGGTACTTCGCTTGTACATGATCACTCGTg                                                                                                    >  2:2298575/1‑150 (MQ=255)

                                                   gCAGGGTATGCTCGAGCAGTTGGCGCTCGACGCGCTCCTCATGGTCGCGGCGCTGGGACATCTGGTTGGCGCAGCCGCTGACGAGCTGGGCGCCGCACAGTGCGGCGCCCCCAAGGGTAAAGGTACTTCGCTTGTACATGATCACTCGTg                                                                                                    <  1:9073002/150‑1 (MQ=255)

                                                    cAGGGTATGCTCGAGCAGTTTGCGCTCGACGCGCTCCTCTTGGTCGGTGCGCTGGGACATCTGGTTGGCGCAGCGGCTGACGAGCCGGGCGCCGCACCGTGCGGCGCCCCCAAGGGTAAAGGTACTTCGCTTGTACATGATCACTCGTGc                                                                                                   <  1:518688/150‑1 (MQ=255)

                                                       ggTATGCTCTAGCTGTTTGCGCTCGACGGGAACCTATTGGTCGCTGGGGTGGTACTTCTGTTTGGCGCCGCCGCTGACGCGCCGGGCGCCGCACCGGGGGGCGCCCCCAAGGGTAAAGGTACTTCGCTTGTACATGATCACTCGTGCAtt                                                                                                <  1:1545816/150‑1 (MQ=255)

                                                       ggTATGCTCGAGCTGTTTGCGCTCTACGGGCTCCTCTTGGTCGGTGGGCTGGGACTTCTGGTGGGCGCCGCCGCTGACGCCCTGGGCGCCGCACTGGGCGGCGCCCCCAAGGGTAAAGGTACTTCGCTTGTACATGATCACTCGTGCAtt                                                                                                <  1:5350605/150‑1 (MQ=255)

                                                        gtagccTCGAGCTGTTGGCGCTCGACGCGCTCCTCATGGTCGCGGCGCTGGGACATCTGGTGGGCGCAGCCGCTGCCGAGCAGGGCGCCGCACTGTGCGGCGCCCCCAAGGGTAAAGGTACTTCGCTTGTACATGATCACTCGTGCATTg                                                                                               <  1:134995/145‑1 (MQ=255)

                                                        gTATGCTCGAGCAGTTTGCGCTCGACGCGCTCCTCATGGTCGCTGCGCTGGGACATCTGGTTGGCGCAGCCGCTGACGAGCAGGGCGCCGCACAGTGCGGCGCCCCCAAGGGTAAAGGTACTTCGCTTGTACATGATCACTCGTGCATTg                                                                                               <  1:7605256/150‑1 (MQ=255)

                                                         tATGCTCGAGCAGTTTGCGCTCGACGCGCTCCTCATGGTCGCTGCGCTGGGACATCTGGTTGGCGCAGCCGCTGACGAGCAGGGCGCCGCACAGTGCGGCGCCCCCAAGGGTAAAGGTACTTCGCTTGTACATGATCACTCGTGCATTgg                                                                                              <  2:675207/150‑1 (MQ=255)

                                                            gCTCGAGCAGTTTGCGCTCGACGCGCTCCTCATGGTCGCTGCGCTGGGACATCTGGTTGGCGCAGCCGCTGACGAGCAGGGCGCCGCACAGTGCGGCGCCCCCAAGGGTAAAGGTACTTCGCTTGTACATGATCACTCGTGCATTGGTTa                                                                                           >  2:619747/1‑150 (MQ=255)

                                                            gCTCGAGCAGTTTGCGCTCGACGCGCTCCTCATGGTCGCTGCGCTGGGACATCTGGTTGGCGCAGCCGCTGACGAGCAGGGCGCCGCACAGTGCGGCGCCCCCAAGGGTAAAGGTACTTCGCTTGTACATGATCACTCGTGCATTGGTTa                                                                                           >  2:1573906/1‑150 (MQ=255)

                                                              tctaGCAGTTTGCGCTCGACGCGCTCCTCATGGTCGCTGCGCTGGGACATCTGGTTGGCGCAGCCGCTGACGAGCAGGGCGCCGCCCCGTGCGGCGCCCCCCAGGGTAAAGGTACTTCGCGTGTTCATGATCACTCGTGCAGTGGTTATc                                                                                         >  1:1674043/4‑150 (MQ=255)

                                                              tCGAGCAGTTTGCGCTCGACGCGCTCCTCATGGTCGCTGCGCTGGGACATCTGGTTGGCGCAGCCGCTGACGAGCAGGGCGCCGCACAGTGCGGCGCCCCCAAGGGTAAAGGTACTTCGCTTGTACATGATCACTCGTGCATTGGTTATc                                                                                         <  2:8249188/150‑1 (MQ=255)

                                                                 aGCAGTTTGCGCTCGACGCGCTCCTCATGGTCGCTGCGCTGGGACATCTGGTTGGCGCAGCCGCTGACGAGCTGGGCGCCGCACTGTGCGGCGCCCCCAAGGGTAAAGGTACTTCGCTTGTACATGATCACTCGTGCATTGGTTATCAgc                                                                                      <  1:4276048/150‑1 (MQ=255)

                                                                 aGCAGTTTGCGCTCGACGCGCTCCTCATGGTCGCTGCGCTGGGACATCTGGTTGGCGCAGCCGCTGACGAGCAGGGCGGCGCACAGTGCGGCGCCCCCCAGGGTAAAGGTTCTTCGCGTGTTCAATATCACTCGTGCAGTGGTTTTCAgc                                                                                      >  1:7055077/1‑150 (MQ=255)

                                                                  gCAGTTTGCGCTCGACGCGCACCTCCTGGTCGCTGCGCTGGGAAATCTGGTTGGCGCAGCGGCTGACGAGCAGGGCGCCGCACAGTGCGGCGCCCCCAAGGGTAAAGGTACTTCGCTTGTACATGATCACTCGTGCATTGGTTATCAgcg                                                                                     <  1:4779278/150‑1 (MQ=255)

                                                                    aGTTTGCGCTCGACGCGCTCCTCATGGTCGCTGCGCTGGGACATCTGGTTGGCGCAGCCGCTGACGAGCAGGGCGCCGCACAGTGCGGCGCCCCCCAGGGTAAAGGTACATAGCGTGTAAATGATCACTCGTGCCTTTGTTATAAgcggc                                                                                   >  1:8690299/1‑150 (MQ=255)

                                                                      tttGCGCTCGACGCGCTCCTCATGGTCGCTGCGCTGGGACATCTGGTTGGCGCAGCCGCTGACGAGCAGGGCGCCGCACAGTGCGGCGCCCCCAAGGGTAAAGGTACTTCGCTTGTACATGATCACACCTGCATTGGTTATCAGCGGCGa                                                                                 >  1:5808510/1‑150 (MQ=255)

                                                                        tGCGCTCGACGCGCTCCTCATGGTCGCTGCGCTGGGACATCTGGTTGGCGCAGCCGCTGACGAGCAGGGCGCCGCACAGTGCGGCGCCCCCAAGGGTAAAGGTACTTCGCTTGTACATGATCACTCGTGCATTGGTTATCAGCGGCGAAc                                                                               <  1:6798546/150‑1 (MQ=255)

                                                                           gcTCGACGCGCTCCTCATGGTCGCTGCGCTGGGACATCTGGTTGGCGCAGCCGCTGACGAGCAGGGCGCCGCACAGTGCGGCGCCCCCAAGGGTAAAGGTACTTCGCTTGTACATGATCACTCGTGGATTGGTTATCAGCGGCGAAcgcg                                                                            >  2:8608254/1‑150 (MQ=255)

                                                                            cccGACGGGCCCCTCTTGGTCGCTGCGCTGGGACATCTGTTTGGCGCAGCCGCTGCCGCGCCGGGCGCCCCACAGTGCGGCGCCCCCAAGGGTAAAGGTACTTCGCTTGTACATGATCACTCGTGCATTGGTTATCAGCGGCGAACGCgg                                                                           <  1:1521125/148‑1 (MQ=255)

                                                                            cTCGACGCGCTCCTCATGGTCGCTGCGCTGGGACATCTGGTTGGCGCAGCCGCTGACGAGCAGGGCGCCGCACAGTGCGGCGCCCCCAAGGGTAAAGGTACTTCGCTTGTACATGATCACTCGTGCATTGGTTATCAGCGGCGAACGCgg                                                                           >  2:4306777/1‑150 (MQ=255)

                                                                            cTCGACGCGCTCCTCATGGTCGCTGCGCTGGGACATCTGGTTGGCGCAGCCGCTGACGAGCAGGGCGCCGCACAGTGCGGCGCCCCCAAGGGTAAAGGTACTTCGCTTGTACATGATCACTCGTGCATTGGTTATCAGCGGCGAACGCgg                                                                           <  2:3169468/150‑1 (MQ=255)

                                                                              cGACGCGCTCCTCTTGGTCGCTGCGCTGGGACTTCTGGTTGGCGCAGCCGCTGACGAGCAGGGCGCCGCACAGTGCGGCGCCCCCAAGGGTAAAGGTACTTCGCTTGTACATGATCACTCGTGCATTGGTTATCAGCGGCGAACGCGGGc                                                                         <  1:6292404/150‑1 (MQ=255)

                                                                              cGACGCGCTCCTCCTGGTCGCTGCGCTGGGACATCTGGTTGGCGCAGCCGCTGACGAGCAGGGCGCCGCACAGTGCGGCGCCCCCAAGGGTAAAGGTACTTCGCTTGTACATGATCACTCGTGCATTGGTTATCAGCGGCGAACGCGGGc                                                                         <  2:8172328/150‑1 (MQ=255)

                                                                                 cgcgCTCCTCATGGTCGCTGCGCTGGGACATCTGGTTGGCGCAGCCGCTGACGAGCAGGGCGCCGCACAGTGCGGCGCCCCCAAGGGTAAAGGTACTTCGCTTGTACATGATCACTCGTGCATTGGTTATCAGCGGCGCACGCGGGCCTg                                                                      >  1:683921/1‑150 (MQ=255)

                                                                                  gcgcTCCTCATGGTCGCTGCGCTGGGACATCTGGTTGGCGCAGCCGCTGACGAGCAGGGCGCCGCACAGTGCGGCGCCCCCCAGGGTAAAGGTACTTCGCGTGTACATGATCACTCGTGCATTGGTTATCAGCGGCGAACGCGGGCCTGc                                                                     >  1:1299865/1‑150 (MQ=255)

                                                                                     ctcctcATGGTCGCTGCGCTGGGACATCTGGTTGGCGCAGCCGCTGACGAGCAGGGCGCCGCACAGTGCGGCGCCCCCAAGGGTAAAGGTACTTCGCTTGTACATGATCACTCGTGCATTGGTTATCAGCGGCGAACGCGGGCCTGCAgg                                                                  >  2:1648136/1‑150 (MQ=255)

                                                                                      tcctcATGGTCGCTGCGCTGGGACATCTGGTTGGCGCAGCCGCTGACGAGCAGGGCGGCGGACAGTGCGGCGCCCCCAAGGGTAAATGTACTTGGCGTGTTAATTATCACTCGTTGATTTTTTTTAAGGGGCGAAAGCGGGGCTggagga                                                                 >  1:3368474/1‑150 (MQ=255)

                                                                                       cctcATGGTCGCTGCGCTGGGACATCTGGTTGGCGCAGCCGCTTACGAGCAGGGCGCCCCCCAGTGCGGCGCCCCCCAGGGTAAAGGTTCATAGCGTGTTCAAGATCACGAGGGCCTTTGTTATGAGCCGCGAACGCGGGCCGGCAggga                                                                >  1:6132521/1‑148 (MQ=255)

                                                                                       cctcATGGTCGCTGCGCTGGGACATCTGGTTGGCGCAGCCGCTGACGAGCAGGGCGCCGCACAGTGCGGCGCCCCCAAGGGTAAAGGTACTTCGCTTGTACATGATCACTCGTGCATTGGTTATCAGCGGCGAACGCGGGCCTGCAGGaa                                                                <  1:639159/150‑1 (MQ=255)

                                                                                        ctcATGGTCGCTGCGCTGGGACATCTGGTTGGCGCAGCCGCTGACGAGCAGGGCGCCGCACAGTGCGGCGCCCCCAAGGGTAAAGGTACTTCGCTTGTACATGATCACTCGTGCATTGGTTATCAGCGGCGAACGCGGGCCTGCAGGAAg                                                               >  2:2980431/1‑150 (MQ=255)

                                                                                        ctcATGGTCGCTGCGCTGGGACATCTGGTTGGCGCAGCCGCTGACGAGCAGGGCGCCGCACAGTGCGGCGCCCCCAAGGGTAAAGGTACTTCGCTTGTACATGATCACTCGTGCATTGGTTATCAGCGGCGAACGCGGGCCTGCAGGAAg                                                               >  2:8763988/1‑150 (MQ=255)

                                                                                        ctcATGGTCGCTGCGCTGGGACATCTGGTTGGCGCAGCCGCTGACGAGCAGGGCGCCGCACAGTGCGGCGCCCCCAAGGGTAAACGTACATCGCTTGTACATGATCACTCGTGCATTGGTTATCACCGGCCAACCCCGGCCAgaaggaag                                                               >  1:8165730/1‑150 (MQ=255)

                                                                                           tcagcggctgcgctgGGACATCTGGTTGGCGCAGCCGCTGACGAGCAGGGCGCCGCACAGTGCGGCGCCCCCAAGGGTAAAGGTACTTCGCTTGTACATGATCACTCGTGCATTGGTTATCAGCGGCGAACGCGGGCCTGCAGGAAGGTc                                                            >  2:7332354/7‑150 (MQ=255)

                                                                                           aTGGTCGCTGCGCTGGGACATCTGGTTGGCGCAGCCGCTGACGAGCAGGGCGCCGCACAGTGCGGCGCCCCCAAGGGTAAAGGTACTTCGCTTGTACATGATCACTCGTGCCTTGGTTATCAGCGGCGAACGCGGGCCTGCAGGAAGGTc                                                            >  2:6343379/1‑150 (MQ=255)

                                                                                           aTGGTCGCTGCGCTGGGACATCTGGTTGGCGCAGCCGCTGACGAGCAGGGCGCCGCACAGTGCGGCGCCCCCAAGGGTAAAGGTACTTCGCTTGTACATGATCACTCGTGCATTGGTTATCAGCGGCGAACGCGGGCCTGCAGGAAGGTc                                                            >  2:7812233/1‑150 (MQ=255)

                                                                                           aTGGTCGCTGCGCTGGGACATCTGGTTGGCGCAGCCGCTGACGAGCAGGGCGCCGCACAGTGCGGCGCCCCCAAGGGTAAAGGTACTTCGCTTGTACATGATCACTCGTGCATTGGTTATCAGCGGCGAACGCGGGCCTGCAGGAAGGTc                                                            <  2:3377267/150‑1 (MQ=255)

                                                                                           aTGGTCGCTGCGCTGGGACATCTGGTTGGCGCAGCCGCTGACGAGCAGGGCGCCGCACAGTGCGGCGCCCCCAAGGGTAAAGGTACTTCGCTTGTACAAGATCACACGTGCATTGGTTATCAGCGGCGAACGCGGGCCTGCAGGAAGGTc                                                            >  2:516370/1‑150 (MQ=255)

                                                                                            gggTCGCTGCGCTGGGACCTGGGGTGGGCGCCGCCGCGGACGCGCTGGGCGCCGCACCGGGCGGCGCCCCCAAGGGTAAAGGTACTTCGCTTGTACATGATCACTCGTGCATTGGTTATCAGCGGCGAACGCGGGCCTGCAGGAAGGTCa                                                           <  1:5476821/149‑1 (MQ=255)

                                                                                               tCGCTGCGCTGGGACATCTGGTTGGCGCAGCCGCTGACGAGCAGGGCGCCGCACAGTGCGGCGCCCCCAAGGGTAAAGGTACTTCGCTTGTACATGATCACTCGTGCATTGGTTATCAGCGGCGAACGCGGGCCTGCAGGAAGGTCAGCa                                                        >  2:4646884/1‑150 (MQ=255)

                                                                                                   tgcgctgGGACATCTGGTTGGCGCAGCCGCTGACGAGCAGGGCGCCGCACAGTGCGGCGCCCCCAAGGGTAAAGGTACTTCGCTTGTACATGATCACTCGTGCATTGGTTATCAGCGGCGAACGCGGGCCTGCAGGAAGGTCAGCACTTc                                                    >  2:4083461/1‑150 (MQ=255)

                                                                                                   tgcgctgGGACATCTGGTTGGCGCAGCCGCTGACGAGCAGGGCGCCCCACACTGCGGCGCCCCCCACGGTAAAGGTACTTCGCGTGTAAAAGATAACTAGTGCATTGGTTATAAGCGGCGAACGCGGGCCAGCACGAAGGTCAGCACATc                                                    >  1:7715149/1‑150 (MQ=255)

                                                                                                     cgctgGGACATCTGGTTGGCGCAGCCGCTGACGAGCAGGGCGCCGCACAGTGCGGCGCCCCCAAGGGTAAAGGTACTTCGCTTGTACATGATCACTCGTGCATTGGTTATCAGCGGCGAACGCGGGCCTGCAGGAAGGTCAGCACTTCAt                                                  <  2:1753767/150‑1 (MQ=255)

                                                                                                      gctgGGACATCTGGTTGGCGCAGCCGCTGACGAGCAGGGCGCCGCACAGTGCGGCGCCCCCAAGGGTAAAGGTACTTCGCTTGTACATGATCACTCGTGCATTGGTTATCAGCGGCGAACGCGGGCCTGCAGGAAGGTCAGCACTTCAtt                                                 <  1:3496922/150‑1 (MQ=255)

                                                                                                                     tGGCGCAGCCGCTGACGAGCAGGGCGCCGCACAGTGCGGCGCCCCCAAGGGTAAAGGTACTTCGCTTGTACATGATCACTCGTGCATTGGTTATCAGCGGCGAACGCGGGCCTGCAGGAAGGTCAGCACTTCATTGAGCGGCAACGCCTg                                  <  2:9135373/150‑1 (MQ=255)

                                                                                                                      ggCGCAGCCGCTGACGAGCAGGGCGGCGCACAGTGCGGCGCCCCCAAGGGTAAAGGTACTTCGCTTGTACATGATCACTCGTGCATTGGTTATCAGCGGCGAACGCGGGCCTGCAGGAAGGTCAGCACTTCATTGAGCGGCAACGCCTgg                                 <  2:7009265/150‑1 (MQ=255)

                                                                                                                      ggCGCAGCCGCTGACGAGCAGGGCGCCGCACAGTGCGGCGCCCCCAAGGGTAAAGGTACTTCGCTTGTACATGATCACTCGTGCATTGGTTATCAGCGGCGAACGCGGGCCTGCAGGAAGGTCAGCACTTCATTGAGCGGCAACGCCTgg                                 <  2:6537433/150‑1 (MQ=255)

                                                                                                                      ggCGCAGCCGCTGACGAGCAGGGCGCCGCACAGTGCGGCGCCCCCAAGGGTAAAGGTACTTCGCTTGTACATGATCACTCGTGCATTGGTTATCAGCGGCGAACGCGGGCCTGCAGGAAGGTCAGCACTTCATTGAGCGGCAACGCCTgg                                 <  1:958511/150‑1 (MQ=255)

                                                                                                                      ggCGCAGCCGCCGACGAGCAGGGCGCCGCACAGTGCGGCGCCCCCAAGGGTAAAGGTACTTCGCTTGTACATGATCACTCGTGCATTGGTTATCAGCGGCGAACGCGGGCCTGCAGGAAGGTCAGCACTTCATTGAGCGGCAACGCCTgg                                 <  2:2401361/150‑1 (MQ=255)

                                                                                                                             ccGCTGACGAGCAGGGCGCCGCACTGTGCGGCGCCCCCAAGGGTAAAGGTACTTCGCTTGTACATGATCACTCGTGCATTGGTTATCAGCGGCGAACGCGGGCCTGCAGGAAGGTCAGCACTTCATTGAGCGGCAACGCCTGGGCGTCCt                          <  1:5257798/150‑1 (MQ=255)

                                                                                                                                 tGACGAGCAGGGCGCCGCACAGTGCGGCGCCCCCAAGGGTAAAGGTACTTCGCTTGTACATGATAACTCGTGCATTGGTTATCAGCGGCGAACGCGGGCCTGCAGGAAGGTCAGCACTTCATTGAGCGGCAACGCCTGGGCGTCCTGCTc                      >  2:3304075/1‑150 (MQ=255)

                                                                                                                                     gAGCAGGGCGCCCCAAAGTGCGGCGCCCCCAAGGGGAAAGGTACATCGCTTTTAAATGAAAACACGTGCAGTGGTTATAAGCGGCGAAAGCGGGCCTGCAGGAAGGTAAGCACTTCAATGAGCGGCAACGCCTGGGCGTCCATCTcggtg                  >  2:7712919/1‑150 (MQ=255)

                                                                                                                                      ttgggggCGCCGCACTGTGCGGCGCCCTCAAGGGTAAAGGTACTTCGCTTGTACATGATCACTCGTGCATTGGTTATCAGCGGCGAACGCGGGCCTGCAGGAAGGTCAGCACTTCATTGAGCGGCAACGCCTGGGCGTCCTGCTcggtgc                 >  1:7351993/5‑150 (MQ=255)

                                                                                                                                        cAGGGCGCCGCACAGTGCGGCGCCCCCAAGGGTAAAGGTACTTCGCTTGTACATGATCACTCGTGCATTGGTTATCAGCGGCGAACGCGGGCCTGCAGGAAGGTCAGCACTTCATTGAGCGGCAACGCCTGGGCGTCCTGCTcggtgcgg               >  2:4879679/1‑150 (MQ=255)

                                                                                                                                           ggCGCCGCACTGTGCGGCGCCCCCAAGGGTAAAGGTACTTCGCTTGTACATGATCACTCGTGCATTGGTTATCAGCGGCGAACGCGGGCCTGCAGGAAGGTCAGCACTTCATTGAGCGGCAACGCCTGGGCGTCCTGCTCggtgcggtgc            >  2:8050799/1‑150 (MQ=255)

                                                                                                                                           ggCGCCGCACAGTGCGGCGCCCCCAAGGGTAAAGGTACTTCGCTTGTACATGATCACTCGTGCATTGGTTACCAGCGGCGAACGCGGGCCTGCAGGAAGGTCAGCACTTCATTGAGCGGCAACGCCTGGGCGTCCTGCTCggtgcggtgc            <  2:29182/150‑1 (MQ=255)

                                                                                                                                            gcgcCGCACAGTGCGGCGCCCCCAAGGGTAAAGGTACTTCGCTTGTACATGATCACTCGTGCATTGGTTATCAGCGGCGAACGCGGGCCTGCAGGAAGGTCAGCACTTCATTGAGCGGCAACGCCTGGGCGTCCTGCTCGGTGCGGTGCt           >  1:5918289/1‑150 (MQ=255)

                                                                                                                                             cgccgcACAGTGCGGCGCCCCCAAGGGTAAATGTACTTCGCTTGTACATGATCAATCGTGCATTGGTTATCAGCGGCGAACGCGGGCATGCAGGAAGGTCAGCACTTCATTGAGCGGCAACGCCTGGGCGTCCTGCTCGGTGCGGTGCtt          <  2:8789767/150‑1 (MQ=255)

                                                                                                                                             cgccgcACAGTGCGGCGCCCCCAAGGGTAAAGGTACTTCGCTTGTACATGATCACTCGTGCATTGGTTATCAGCGGCGAACGCGGGCCTGCAGGAAGGTCAGCACTTCATTGAGCGGCAACGCCTGGGCGTCCTGCTCGGTGCGGTGCtt          <  2:5467246/150‑1 (MQ=255)

                                                                                                                                              gccgcACAGTGCGGCGCCCCCAAGGGTAAAGGTACTTCGCTTGTACATGATCACTCGTGCATTGGTTATCAGCGGCGAACGCGGGCCTGCAGGAAGGTCAGCACTTCATTGAGCGGCAACGCCTGGGCGTCCTGCTCGGTGCGGTGCTTg         >  2:7469180/1‑150 (MQ=255)

                                                                                                                                              gccgcACAGTGCGGCGCCCCCAAGGGTAAAGGTACTTCGCTTGTACATGATCACTCGTGCATTGGTTATCAGCGGCGAACGCGGGCCTGCAGGAAGGTCAGCACTTCATTGAGCGGCAACGCCTGGGCGTCCTGCTCGGTGCGGTGCTTg         >  2:4999592/1‑150 (MQ=255)

                                                                                                                                               ccgcACAGTGCGGCGCCCCCAAGGGTAAAGGTACTTCGCTTGTACATGATCACTCGTGCATTGGTTATCAGCGGCGAACGCGGGCCTGCAGGAAGGTCAGCACTTCATTGAGCGGCAACGCCTGGGCGTCCTGCTCGGTGCGGTGCTTGt        <  1:4646884/150‑1 (MQ=255)

                                                                                                                                                cgcACAGTGCGGCGCCCCCAAGGGTAAAGGTACTTCGCTTGTACATGATCACTCGTGCATTGGTTATCAGCGGCGAACGCGGGCCTGCAGGAAGGTCAGCACTTCATTGAGCGGCAACGCCTGGGCGTCCTGCTCGGTGCGGTGCTTGTa       <  2:6230056/150‑1 (MQ=255)

                                                                                                                                                    caGTGCGGCGCCCCCAAGGGTAAAGGTACTTCGCTTGTACATGATCACTCGTGCATTGGTTATCAGCGGCGAACGCGGGCCTGCAGGAAGGTCAGCACTTCATTGAGCGGCAACGCCTGGGCGTCCTGCTCGGTGCGGTGCTTGTACTcc   <  2:3612497/150‑1 (MQ=255)

                                                                                                                                                     aGTGCGGCGCCCCCAAGGGTAAAGGTACTTCGCTTGTACATGATCACTCGTGCATTGGTTATCAGCGGCGAACGCGGGCCTGCAGGAAGGTCAGCACTTCATTGAGCGGCAACGCCTGGGCGTCCTGCTCGGTGCGGTGCTTGTACTCCa  <  2:568688/150‑1 (MQ=255)

GCACACGTCGCTGCGGAAGCTCCATCACCTTCGGCTCGCCGACATCGATCTGCAGGGTATGCTCGAGCAGTTTGCGCTCGACGCGCTCCTCATGGTCGCTGCGCTGGGACATCTGGTTGGCGCAGCCGCTGACGAGCAGGGCGCCGCACAGTGCGGCGCCCCCAAGGGTAAAGGTACTTCGCTTGTACATGATCACTCGTGCATTGGTTATCAGCGGCGAACGCGGGCCTGCAGGAAGGTCAGCACTTCATTGAGCGGCAACGCCTGGGCGTCCTGCTCGGTGCGGTGCTTGTACTCCA  >  NC_002947/1383111‑1383409

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`