Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,392,933 C→G 14.5% intergenic (+97/‑89) PP_1214 → / → ruvC transcriptional regulator/crossover junction endodeoxyribonuclease RuvC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,392,9330CG14.5% 560.0 / 66.3 216intergenic (+97/‑89)PP_1214/ruvCtranscriptional regulator/crossover junction endodeoxyribonuclease RuvC
Reads supporting (aligned to +/- strand):  ref base C (85/89);  new base G (16/15);  total (107/109)
Fisher's exact test for biased strand distribution p-value = 8.47e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 217 total aligned reads displayed.

ACGTCTATTCCAATGCCCAGATTTCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACGCC‑CA‑TTTCAA‑ATGTGGTGCAGTACCTGTAGGAGCGGGTTTA‑CCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCACCGGCTATGGCGTGGTACG  >  NC_002947/1392784‑1393080
                                                                                                                                                       |                                                                                                                                                     
 cGTCTATTCCAATGCCCAGATTTCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACGCC‑CA‑TTTCa                                                                                                                                                      <  1:13776/150‑1 (MQ=255)
  gTCTATTCCAATGCCCAGATTTCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACGCC‑CA‑TTTCaa                                                                                                                                                     <  1:1691217/150‑1 (MQ=255)
    cTATTCCAATGCCCAGATTTCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACGCC‑CA‑TTTCAA‑At                                                                                                                                                  <  2:6131025/150‑1 (MQ=255)
    cTATTCCAATGCCCAGATTTCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGGATTACGCC‑CA‑GTTCAA‑At                                                                                                                                                  >  2:5220338/1‑150 (MQ=255)
    cTATTCCAATGCCCAGATTTCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCCCTCCAACAGGTATTAAGCC‑CC‑TTTCAA‑At                                                                                                                                                  >  2:3439545/1‑150 (MQ=255)
    cTATTCCAATGCCCAGATTTCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGACCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACGCC‑CA‑TTTCAA‑At                                                                                                                                                  <  1:997377/150‑1 (MQ=255)
       ttCCAATGCCCAGATTTCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACGCC‑CA‑TTTCAA‑Atgtg                                                                                                                                               <  1:8293440/150‑1 (MQ=255)
       ttCCAATGCCCAGATTTCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACGCC‑CA‑TTTCAA‑Atgtg                                                                                                                                               <  2:3223484/150‑1 (MQ=255)
       ttCCAATGCCCAGATTTCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGGATTACGCC‑CA‑TTTCAA‑Atgtg                                                                                                                                               >  2:6103797/1‑150 (MQ=255)
        tCCAATGCCCAGATTTCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACGCC‑CA‑TTTCAA‑ATgtgg                                                                                                                                              <  2:7144281/150‑1 (MQ=255)
          cAATGCCCAGATTTCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACGCC‑CA‑TTTCAA‑ATgtggtg                                                                                                                                            <  1:5371972/150‑1 (MQ=255)
           aaTGCCCAGATTTCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACGCC‑CA‑TTTCAA‑ATGTGGTGc                                                                                                                                           <  2:8846411/150‑1 (MQ=255)
            aTGCCCAGATTTCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACGCC‑CA‑TTTCAA‑ATGTGGTGCa                                                                                                                                          <  1:6313288/150‑1 (MQ=255)
            aTGCCCAGATTTCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACGCC‑CA‑TTTCAA‑ATGTGGTGCa                                                                                                                                          <  2:4485213/150‑1 (MQ=255)
            aTGCCCAGATTTCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACGCC‑CA‑TTTCAA‑ATGTGGTGCa                                                                                                                                          <  2:3713488/150‑1 (MQ=255)
                ccAGATTTCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACGCC‑CA‑TTTCAA‑ATGTGGTGCAGTAc                                                                                                                                      <  1:6999786/150‑1 (MQ=255)
                  aGATTTCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACGCC‑CA‑TTTCAA‑ATGTGGTGCAGTACCt                                                                                                                                    <  1:4588784/150‑1 (MQ=255)
                    aTTTCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTACTGCACCACA‑TTTGAA‑ATG‑GGCGTAATACCTGt                                                                                                                                  <  2:6470448/150‑1 (MQ=21)
                     tttCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACGCC‑CA‑TTTCAA‑ATGTGGTGCAGTACCTGTa                                                                                                                                 <  1:8638420/150‑1 (MQ=255)
                     tttCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTACTGCACCACA‑TTTGAA‑ATG‑GGCGTAATACCTGTa                                                                                                                                 <  2:6612832/150‑1 (MQ=21)
                     tttCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTACTGCACCACA‑TTTGAA‑ATG‑GGCGTAATACCTGTa                                                                                                                                 <  2:5112705/150‑1 (MQ=21)
                      ttCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACGCC‑CA‑TTTCAA‑ATGTGGTGCAGTACCTGTAg                                                                                                                                <  2:5933822/150‑1 (MQ=255)
                      ttCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACGCC‑CA‑TTTCAA‑ATGTGGTGCAGTACCTGTAg                                                                                                                                <  1:6540923/150‑1 (MQ=255)
                      ttCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACGCC‑CA‑TTTCAA‑ATGTGGTGCAGTACCTGTAg                                                                                                                                <  2:6805808/150‑1 (MQ=255)
                      ttCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACGCC‑CA‑TTTCAA‑ATGTGGTGCAGTACCTGTAg                                                                                                                                <  1:2615634/150‑1 (MQ=255)
                       tCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACGCC‑CA‑TTTCAA‑ATGTGGTGCAGTACCTGTAgg                                                                                                                               <  1:6254066/150‑1 (MQ=255)
                          gacgaGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTACTGCACCACA‑TTTGAA‑ATG‑GGCGTAATACCTGTAGGAGc                                                                                                                            <  1:2944319/150‑1 (MQ=18)
                          gacgaGAACATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGCCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACGCC‑CA‑TTTCAA‑ATGTGGTGCAGTACCTGTAGGAGc                                                                                                                            <  2:2555391/150‑1 (MQ=255)
                            cgaGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACGCC‑CA‑TTTCAA‑ATGTGGTGCAGTACCTGTAGGAGCgg                                                                                                                          <  1:767000/150‑1 (MQ=255)
                            cgaGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACGCC‑CA‑TTTCAA‑ATGTGGTGCAGTACCTGTAGGAGCgg                                                                                                                          <  1:767167/150‑1 (MQ=255)
                             gagaTCATGGAAAACCTCGGCTAAGGTGATTTGGCTTTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTACTGCACCACA‑TTTGAA‑ATG‑GGCGTAATACCTGTAGGAGCggg                                                                                                                         <  1:3869268/150‑1 (MQ=18)
                             gagaTCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACGCC‑CA‑TTTCAA‑ATGTGGTGCAGTACCTGTAGGAGCggg                                                                                                                         <  1:7313638/150‑1 (MQ=255)
                               gaTCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACGCC‑CA‑TTTCAA‑ATGTGGTGCAGTACCTGTAGGAGCGGGtt                                                                                                                       <  1:4036150/150‑1 (MQ=255)
                               gaTCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACGCC‑CA‑GTTCAA‑ATGTGGTGCAGTACCTGGAGGAGCGGGtt                                                                                                                       >  2:9023992/1‑150 (MQ=255)
                                 tCATGGAGAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACGCC‑CA‑TTTCAA‑ATGTGGTGCAGTACCTGTAGGAGCGGGTTTa                                                                                                                     <  1:3629829/150‑1 (MQ=255)
                                  cATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACGCC‑CA‑TTTCAA‑ATGTGGTGCAGTACCTGTAGGAGCGGGTTTA‑c                                                                                                                   <  1:7596504/150‑1 (MQ=255)
                                  cATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACGCC‑CA‑TTTCAA‑ATGTGGTGCAGTACCTGTAGGAGCGGGTTTA‑c                                                                                                                   <  2:7821861/150‑1 (MQ=255)
                                   aTGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTTAACCCGCGCCTACCGGTATTACGCC‑CC‑ATTCAA‑AAGTGGTGGAGGACCAGGAGGAGCGGGGTTT‑cc                                                                                                                  >  2:211680/1‑150 (MQ=255)
                                   aTGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTGAAACCGCTCCTACAGGTATTACGCC‑CA‑AATGAA‑ATGTTGTGCAGTACCAGTGGGAGCGGGGTTagc                                                                                                                   >  1:6794463/1‑148 (MQ=38)
                                   aTGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACGCC‑CA‑TTTCAA‑ATGTGGTGCAGTACCTGTAGGAGCGGGTTTA‑cc                                                                                                                  <  2:544691/150‑1 (MQ=255)
                                   aTGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCGTTCGCGGGGAAAACCGCGCCAAAAGGTATTACGCC‑CC‑ATTGAA‑AAGTGGTGCCGTAACGGGTGGGGCGGGGTTagc                                                                                                                   >  1:6794609/1‑148 (MQ=255)
                                    tGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAAACCCCTCCTACAGGTATTACGCC‑CA‑TTTCAA‑ATGTGGTGCAGTACCGGTAGGAGCGGGTTTT‑ccc                                                                                                                 >  2:4515610/1‑150 (MQ=255)
                                            cTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGGAAACCCCCGGCTAAAGGTGTTAGGCC‑CC‑TTTCAA‑ATGTGGTGCGGGACCGGGTGGGGGGGGGTTA‑GCCGCCGAAgg                                                                                                         >  2:7709820/1‑150 (MQ=255)
                                              cGGCTATGGTGATTGGGCTTTTTGTGCCAACTCTGAGCGTTTGATAGCGCGTGTGCGGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTACTGCACCACA‑TTTTAA‑CTG‑GGCTTAATACCTGTAGGCGCGGGTTTA‑CCCGCGAAAGGGc                                                                                                       <  2:4759867/150‑1 (MQ=14)
                                               ggCTATGGTGTTTTGGCATTTGGTGCCAACTCTGAGGGTTTGATAGCGCCTGTGCGGGCCCTTTCGGGGTTAAACCCGCTCCTACAGGTATTACGCC‑CA‑TTTCAA‑ATGTGGTGCAGTACCTGTAGGAGGGGGTTTA‑CCCGCGAAAGGGcc                                                                                                      <  2:5773311/150‑1 (MQ=37)
                                                gCTTAGGTGTTTTGGCTTTTTGTGCCAACTCTGAGCGTTTGTTAGCGCCTGTGCCGGCCCTTTCGCGGTTAAACCCGCTCCTACCGGTATTACGCC‑CA‑TTTCAA‑ATGTGGTGCAGTACCTGTAGGAGCGGGTTTA‑CCCGCGAAAGGGCCg                                                                                                     <  2:4795251/150‑1 (MQ=37)
                                                  tAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACGCC‑CA‑TTTCAA‑ATGTGGTGCAGTACCGGTAGGAGCGGGTGTA‑ACCCCGAAAGGGCCGgc                                                                                                   >  2:4483738/1‑150 (MQ=255)
                                                                aTTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAAACCGCTCCAAAAGGTATTACGCC‑CC‑ATTCAA‑ATGTGGTGCAGTACCGGTAGGAGCGGGTTTA‑CCCGCGAAAGGGGCGGCGCAGTTTGTAgaga                                                                                     >  2:2907118/1‑150 (MQ=37)
                                                                         cAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCACCTACAGGTATTACGCC‑CA‑TTTAAA‑AAGTGGTGCAGTACCAGTAGGAGCCGGTTTA‑CCCCCGGAAGGGCCGGCGCAGGTTGTAGAGATGCATCCtt                                                                            >  2:3690749/1‑150 (MQ=37)
                                                                         cAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTAGCGGGTAAAACCCCGCCAAAACGTATTAAGCC‑CA‑ATTAAA‑AATTGGTGCCGTACCCGTGGGAGCGGGGTTA‑CCCGCGCAAGGGCCGGCGCCGTGTGTGGTGCTTCATCAtt                                                                            >  2:163042/1‑150 (MQ=255)
                                                                            ctctGCGCTTTTTATCGCGCGTGGCCCGGCCCTTTCGGGGTAAAACCCGGTCCTAC‑CGTTTTTCCCC‑CA‑TTTAAATTTTTGGTGCATTACATTTCGTCGGGGTTTAA‑CCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCAttttt                                                                         <  2:4894583/150‑1 (MQ=255)
                                                                                     tttGATCGCGCCTGTGCCGGCCCTTTCGCGGTTAAACCCGCTCCTCCCGTTTTTACGCC‑CA‑TTTAAA‑ATGTGGTGCAGTACCTGTAGGAGCGGTTTTA‑CCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGc                                                                <  2:2887896/150‑1 (MQ=37)
                                                                                     tttGATAGCGCCTGTGCCGGCCCGTTCGCGGGTAAAACCCCACCAACACGTAATAAGCC‑CA‑ATTAAA‑ATGTTGTGCACTACCGGTAGGAGCGGGTTTA‑CCCGCGACAGGGCCCGCGCAGGTTGTAGAGATTGAACAATTTTTCggacggc                                                                >  2:514124/1‑144 (MQ=19)
                                                                                         aTAGCGCCTGTGCCGGCCCTTTCGCGGGGAAACCCCCCCCAACCGGTATTAACCC‑CC‑CTTCAA‑AATGGGGGCCGTACCGGGCGGAGCCGGGTTA‑CCCGCCAAAGGGCCGGGGGAGGTTGGTTCGCTGGATCAATTTTTTGAAGGGCgttt                                                            >  2:4241734/1‑149 (MQ=255)
                                                                                            gcggCTGTGCCGCCCCTTTCGCGGTTAACCCCGATCCCACCGGTCTCACCCC‑CA‑TTTCAC‑ATGTGGTGCAGTACCTGTAGGTGGGGTTTTA‑CCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGa                                                         <  2:8768650/150‑1 (MQ=37)
                                                                                               ccTGTGCCGGCCCTTTCGCGGGTAAACCCCCTCCTACAGGTATTAAGCC‑CC‑TTTGAA‑ATGGGGGGCAGTAACGGTAGGAGCGGGGTTA‑ACCGCGAAAGGGCCCGCGCCGGTTGTAGAGAAGCCTCAATTTTTAGACGGGGGTTATGActc                                                      >  2:8121291/1‑150 (MQ=18)
                                                                                                       ggCCCTTGCGCGGGTAACCCCGCTCCTACAGGTATTACGCC‑CA‑TTTCAA‑TTGTGGTGCCGTACCTGTAGGAGCGGGTTTA‑CCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTa                                              <  2:8970281/150‑1 (MQ=255)
                                                                                                       gccccTTCCGGGGTTAACCCCGCCCCTACCGTTA‑TACCCC‑CATTTTCAA‑TGGTGGTGCTGTACGTTTTGGCGGGGTTTTA‑CCCGCGAACGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTa                                              <  2:3991863/148‑1 (MQ=255)
                                                                                                        gCCCTTTCGGGGTTAAACCCGCCCCTTCAGTTATTACGCC‑AT‑TTTCAA‑CTGTGGTTCAGTACCTGTTGGAGCGGGTTTA‑CCCGCGAATGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAg                                             <  2:5833729/150‑1 (MQ=38)
                                                                                                          ccTTTAGCGGGTAAAACCGCACCAAAAGGTAATAAGCC‑CA‑TTTCAA‑ATGTGGTGCAGTAACTGTAGGAGCCGGTTTA‑ACCCCGAAAGGGCCGGCGCAGTTTGTAGAGATTCATCATTTTTTCGACGGGCGTTAAGACAATGTTTCGAGGt                                           >  2:3786890/1‑150 (MQ=255)
                                                                                                                gcgGGTAAACCCGCTCCTACAGGTATTACGCC‑CA‑TTTCAA‑ATGTGGTGCAGTACCTGTAGGAGCGGGTTTA‑CCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGAc                                     >  1:4746087/1‑150 (MQ=255)
                                                                                                                gcgGGTAAACCCGCTCCTACAGGTATTACGCC‑CA‑TTTCAA‑ATGTGGTGCAGTACCTGTAGGAGCGGGTTTA‑CCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGAc                                     >  2:6891310/1‑150 (MQ=255)
                                                                                                                  gggTAAACCCGCTCCTACAGGTACTGCACCACA‑TTTGAA‑ATG‑GGCGTAATACCTGTAGGAGCGGGTTTA‑CCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGAccc                                   >  1:3357009/1‑150 (MQ=17)
                                                                                                                     tAAACCCGCTCCTACAGGTATTACGCC‑CA‑TTTCAA‑ATGTGGTGCAGTACCTGTAGGAGCGGGTTTA‑CCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCgg                                >  1:6156676/1‑150 (MQ=255)
                                                                                                                        aCCCGCTCCTACAGGTATTACGCC‑CA‑TTTCAA‑ATGTGGTGCAGTACCTGTAGGAGCGGGTTTA‑CCCGCGAAAGGGCGGGCGCAGTTTGTAGAGATGCATCATTTTTTCGAGGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTc                             >  2:733015/1‑150 (MQ=255)
                                                                                                                         cccGCTCCTACAGGTATTACGCC‑CA‑TTTCAA‑ATGTGGTGCAGTACCTGTAGGAGCGGGTTTA‑CCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTcg                            >  2:6366017/1‑150 (MQ=255)
                                                                                                                          ccGCTCCTACAGGTATTACGCC‑CC‑TTTCAA‑ATGTGGTGCAGTACCTGTAGGAGCGGGTTTA‑CCCGCGAAAGGGCCGGCGCAGTTTGTCGCGCTGCATCATTTTTTCGACGGGCTGTATGACTCTGATTCTAGGTATCGACCCCGGCTcgc                           >  2:7003043/1‑150 (MQ=38)
                                                                                                                          ccGCTCCTACAGGTATTACGCC‑CA‑TTTCAA‑ATGTGGTGCAGTACCTGTAGGAGCGGGTTTA‑CCCGCGACAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTcgc                           >  1:3020454/1‑150 (MQ=255)
                                                                                                                          ccGCTCCTACAGGTATTACGCC‑CA‑TTTCAA‑ATGTGGTGCAGTACCTGTAGGAGCGGGTTTA‑CCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTcgc                           >  1:2553293/1‑150 (MQ=255)
                                                                                                                          ccGCTCCTACAGGTACTGCACCACA‑TTTGAA‑ATG‑GGCGTAATACCTGTAGGAGCGGGTTTA‑CCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTcgc                           >  2:3534747/1‑150 (MQ=18)
                                                                                                                           cGCTCCTACAGGTCTTACGCC‑CA‑TTTCAA‑ATGTGGTGCAGTACCTGTAGGAGCGGGTTTA‑CCCGCGACAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTcgcg                          >  2:6302244/1‑150 (MQ=255)
                                                                                                                            gCTCCTACAGGTATTACGCC‑CA‑TTTCAA‑ATGTGGTGCAGTACCTGTAGGAGCGGGTTTA‑CCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCgcgc                         >  1:8871883/1‑150 (MQ=255)
                                                                                                                                cTACAGGTACTGCACCACA‑TTTGAA‑ATG‑GGCGTAATACCTGTAGGAGCGGGTTTA‑CCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTAt                                                           >  1:1307246/1‑112 (MQ=11)
                                                                                                                                 tACAGGTATTACGCC‑CA‑TTTCAA‑ATGTGGTGCAGTACCTGTAGGAGCGGGTTTA‑CCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCAc                    >  2:5203473/1‑150 (MQ=255)
                                                                                                                                 tACAGGTATTACGCC‑CA‑TTTCAA‑ATGTGGTGCAGTACCTGTAGGAGCGGGTTTA‑CCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCAc                    >  1:4506199/1‑150 (MQ=255)
                                                                                                                                 tACAGGTATTACGCC‑CA‑TTTCAA‑ATGTGGTGCAGTACCTGTAGGAGCGGGTTTA‑CCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCAc                    >  2:437600/1‑150 (MQ=255)
                                                                                                                                 tACAGGTACTGCACCACA‑TTTGAA‑ATG‑GGCGTAATACCTGTAGGAGCGGGTTTA‑CCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCAc                    >  2:4313744/1‑150 (MQ=21)
                                                                                                                                 tACAGGTACTGCACCACA‑TTTGAA‑ATG‑GGCGTAATACCTGTAGGAGCGGGTTTA‑CCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCAc                    >  2:7232081/1‑150 (MQ=21)
                                                                                                                                 tACAGGTACTGCACCACA‑TTTGAA‑ATG‑GGCGTAATACCTGTAGGAGCGGGTTTA‑CCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCAc                    >  1:7886447/1‑150 (MQ=21)
                                                                                                                                  aCAGGTATTACGCC‑CA‑TTTCAA‑ATGTGGTGCAGTACCTGTAGGAGCGGGTTTA‑CCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCAcc                   >  2:3840291/1‑150 (MQ=255)
                                                                                                                                  aCAGGTATTACGCC‑CA‑TTTCAA‑ATGTGGTGCAGTACCTGTAGGAGCGGGTTTA‑CCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCAcc                   >  1:7359071/1‑150 (MQ=255)
                                                                                                                                    aGGTATTACGCC‑CA‑TTTCAA‑ATGTGGTGCAGTACCTGTAGGAGCGGGTTTA‑CCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCACCgg                 >  2:5618554/1‑150 (MQ=255)
                                                                                                                                    aGGTATTACGCC‑CA‑TTTCAA‑ATGTGGTGCAGTACCTGTAGGAGCGGGTTTA‑CCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCACCgg                 >  2:1069864/1‑150 (MQ=255)
                                                                                                                                    aGGTATTACGCC‑CA‑TTTCAA‑ATGTGGTGCAGTACCTGTAGGAGCGGGTTTA‑CCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCACCgg                 >  1:8474856/1‑150 (MQ=255)
                                                                                                                                      gTATTACGCC‑CA‑TTTCAA‑ATGTGGTGCAGTACCTGTAGGAGCGGGTTTA‑CCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCACCGGCt               >  1:1105/1‑150 (MQ=255)
                                                                                                                                       tATTACGCC‑CA‑TTTCAA‑ATGTGGTGCAGTACCTGTAGGAGCGGGTTTA‑CCCGCGAAAGGGCCGGCGCAGTTTGTa                                                                                         <  1:5613398/75‑1 (MQ=255)
                                                                                                                                         tcaCGCC‑CT‑TTACAC‑CTGGGGTGCAGTCCCTGTAGCCG‑GGGTTAACCCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCACCGGCTATg            <  1:5208359/148‑1 (MQ=255)
                                                                                                                                          tgcaccacA‑TTTGAA‑ATG‑GGCGTAATACCTGTAGGAGCGGGTTTA‑CCCGCGAAAGGGCCGGCACAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCACCGGCTATgg           >  1:7466871/8‑150 (MQ=25)
                                                                                                                                             gCC‑CA‑TTTCAA‑ATGTGGTGCAGTACCTGTAGGAGCGGGTTTA‑CCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTa                                              <  1:838999/112‑1 (MQ=255)
                                                                                                                                             gCC‑CA‑TTTCAA‑ATGTGGTGCAGTACCTGTAGGAGCGGGTTTA‑CCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTa                                              >  2:838999/1‑112 (MQ=255)
                                                                                                                                             gCC‑CA‑TTTCAA‑ATGTGGTGCAGTACCTGTAGGAGCGGGTTTA‑CCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCACCGGCTATGGCGt        >  1:6900087/1‑150 (MQ=255)
                                                                                                                                              cc‑cA‑TTTCAA‑ATGTGGTGCAGTACCTGTAGGAGCGGGTTTA‑CCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCACCGGCTATGGCGTg       <  1:1069806/150‑1 (MQ=255)
                                                                                                                                               c‑cA‑TTTCAA‑ATGTGGTGCAGTACCTGTAGGAGCGGGTTTA‑CCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCACCGGCTATGGCGTgg      >  2:2353434/1‑150 (MQ=255)
                                                                                                                                               c‑cA‑TTTCAA‑ATGTGGTGCAGTACCTGTAGGAGCGGGTTTA‑CCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCACCGGCTATGGCGTgg      >  2:8073981/1‑150 (MQ=255)
                                                                                                                                                 cA‑TTTCAA‑ATGTGGTGCAGTACCTGTAGGAGCGGGTTTA‑ACCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATAGACACCGGCTCGCGCATCACCGGCTATGGCGTGGt     >  2:8152749/1‑150 (MQ=255)
                                                                                                                                                    tttCAA‑ATGTGGTGCAGTACCTGTAGGAGCGGGTTTA‑CCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCACCGGCTATGGCGTGGTAc   >  2:8035081/1‑150 (MQ=255)
                                                                                                                                                    tttCAA‑ATGTGGTGCAGTACCTGTAGGAGCGGGTTTA‑CCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCACCGGCTATGGCGTGGTAc   >  2:5277580/1‑150 (MQ=255)
                                                                                                                                                    tttCAA‑ATGTGGTGCAGTACCTGTAGGAGCGGGTTTA‑CCCGCGAAAGGGCCGGCGCAGTTTGTAAAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCACCGGCTATGGCGTGGTAc   >  1:2163319/1‑150 (MQ=255)
                                                                                                                                                     ttCAA‑ATGTGGTGCAGTACCTGTAGGAGCGGGTTTA‑CCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCACCGGCTATGGCGTGGTACg  >  2:456987/1‑150 (MQ=255)
                                                                                                                                                     ttCAA‑ATGTGGTGCAGTACCTGTAGGAGCGGGTTTA‑CCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCACCGGCTATGGCGTGGTACg  >  2:5111404/1‑150 (MQ=255)
                                                                                                                                                       |                                                                                                                                                     
ACGTCTATTCCAATGCCCAGATTTCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACGCC‑CA‑TTTCAA‑ATGTGGTGCAGTACCTGTAGGAGCGGGTTTA‑CCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCACCGGCTATGGCGTGGTACG  >  NC_002947/1392784‑1393080

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑