BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	14,838	T→C	6.0%	V503V (GTT→GTC)	gyrB →	DNA gyrase subunit B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	14,838	0	T	C	6.0%	796.8 / 24.4	249	V503V (GTT→GTC)	gyrB	DNA gyrase subunit B
Reads supporting (aligned to +/- strand): ref base T (114/120); new base C (8/7); total (122/127)
Fisher's exact test for biased strand distribution p-value = 7.94e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.77e-01

Only 100 of 252 total aligned reads displayed.

AACGTCGAGAAAGCGCGCTTCGACAAGATGATTTCGTCCCAGGAAGTGGGCACGCTGATCACTGCGCTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGA  >  NC_002947/14689‑14984

aaCGTCGAGAAAGCGCGCTTCGACAAGATGATTTCGTCCCAGGAAGTGGGCACGCTGATCACTGCGCTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGtt                                                                                                                                                    >  1:3928035/1‑150 (MQ=255)

    tCGAGAAAGCGCGCTTCGACAAGATGATTTCGTCCCAGGAAGTGGGCACGCTGATCACTGCGCTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACg                                                                                                                                                <  2:2067819/150‑1 (MQ=255)

      gagaAAGCGCGCTTCGACAAGATGATTTCGTCCCAGGAAGTGGGCACGCTGATCACTGCGCTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGt                                                                                                                                              >  2:3279120/1‑150 (MQ=255)

        gaAAGCGCGCTTCGACAAGATGATTTCGTCCCAGGAAGTGGGCACGCTGATCACTGCGCTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTc                                                                                                                                            <  2:1664614/150‑1 (MQ=255)

        gaAAGCGCGCTTCGACAAGATGATTTCGTCCCAGGAAGTGGGCACGCTGATCACTGCGCTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTc                                                                                                                                            <  1:2954225/150‑1 (MQ=255)

         aaaGCGCGCTTCGACAAGATGATTTCGTCCCAGGAAGTGGGCACGCTGATCACTGCGCTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCg                                                                                                                                           >  1:407007/1‑150 (MQ=255)

                  ttCGACAAGATGATTTCGTCCCAGGAAGTGGGCACGCTGATCACTGCGCTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGt                                                                                                                                  >  2:1414151/1‑150 (MQ=255)

                     gACAAGATGATTTCGTCCCAGGAAGTGGGCACGCTGATCACTGCGCTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACg                                                                                                                               >  1:2783563/1‑150 (MQ=255)

                         aGATGATTTCGTCCCAGGAAGTGGGCACGCTGATCACTGCGCTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACgctgc                                                                                                                           >  1:2927543/1‑150 (MQ=255)

                          gatgatTTCGTCCCAGGAAGTGGGCACGCTGATCACTGCGCTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGca                                                                                                                                         >  2:5837921/1‑135 (MQ=255)

                          gatgatTTCGTCCCAGGAAGTGGGCACGCTGATCACTGCGCTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGca                                                                                                                                         <  1:5837921/135‑1 (MQ=255)

                           atgatTTCGTCCCAGGAAGTGGGCACGCTGATCACTGCGCTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACgctgctg                                                                                                                         <  2:4736047/150‑1 (MQ=255)

                           atgatTTCGTCCCAGGAAGTGGGCACGCTGATCACTGCGCTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACgctgctg                                                                                                                         <  1:7295391/150‑1 (MQ=255)

                             gatTTCGTCCCAGGAAGTGGGCACGCTGATCACTGCGCTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACgctgctgct                                                                                                                       >  1:2440715/1‑150 (MQ=255)

                              atTTCGTCCCAGGAAGTGGGCACGCTGATCACTGCGCTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGctgctgctg                                                                                                                      <  2:9081341/150‑1 (MQ=255)

                                ttCGTCCCAGGAAGTGGGCACGCTGATCACTGCGCTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGAc                                                                                                                    >  2:5892875/1‑150 (MQ=255)

                                    tCCCAGGAAGTGGGCACGCTGATCACTGCGCTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACcttc                                                                                                                <  2:2377229/150‑1 (MQ=255)

                                    tCCCAGGAAGTGGGCACGCTGATCACTGCGCTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACcttc                                                                                                                <  2:8657999/150‑1 (MQ=255)

                                    tCCCAGGAAGTGGGCACGCTGATCACTGCGCTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACcttc                                                                                                                <  1:7896235/150‑1 (MQ=255)

                                       cacgAAGTGCGCACGCTGATCACTGCGCTGCGCTGTGCCATCGCCCGCGAACACTACAACATCGACAAACTGCGTTATCACAACAACAACATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTACTGCTGACcttcttc                                                                                                             <  2:4574647/147‑1 (MQ=255)

                                        aGGAAGTGGGCACGCTGATCACTGCGCTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACcttcttct                                                                                                            >  2:4032189/1‑150 (MQ=255)

                                         ggAAGTGGGCACGCTGATCACTGCGCTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACcttcttctt                                                                                                           <  1:8340402/150‑1 (MQ=255)

                                          gAAGTGGGCACGCTGATCACTGCGCTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCttcttcttc                                                                                                          <  1:8965372/150‑1 (MQ=255)

                                           aaGTGGGCACGCTGATCACTGCGCTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTcc                                                                                                         <  2:8061398/150‑1 (MQ=255)

                                            aGTGGGCACGCTGATCACTGCGCTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACctt                                                                                                                 >  2:7416311/1‑141 (MQ=255)

                                                ggCACGCTGATCACTGCGCTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAg                                                                                                    <  1:4731546/150‑1 (MQ=255)

                                                 gCACGCTGATCACTGCGCTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGc                                                                                                   <  2:3012211/150‑1 (MQ=255)

                                                  cACGCTGATCACTGCGCTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCt                                                                                                  <  2:3863366/150‑1 (MQ=255)

                                                   aCGCTGATCACTGCGCTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCGTCTTCTTCCGTCAGCTg                                                                                                 >  1:8259758/1‑150 (MQ=255)

                                                       tGATCACTGCGCTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCgg                                                                                             <  1:6171190/150‑1 (MQ=255)

                                                        gATCACTGCGCTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGa                                                                                            >  1:4302789/1‑150 (MQ=255)

                                                        gATCACTGCGCTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGa                                                                                            <  2:8253458/150‑1 (MQ=255)

                                                           cACTGCGCTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCt                                                                                         >  1:1181980/1‑150 (MQ=255)

                                                             cTGCGCTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTgg                                                                                       >  2:5313370/1‑150 (MQ=255)

                                                                 gcTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGa                                                                                   <  1:204404/150‑1 (MQ=255)

                                                                   tGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGc                                                                                 <  1:4495833/150‑1 (MQ=255)

                                                                   tGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGc                                                                                 <  1:2273544/150‑1 (MQ=255)

                                                                     ggCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGt                                                                               <  1:734124/150‑1 (MQ=255)

                                                                     ggCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGt                                                                               >  2:1788058/1‑150 (MQ=255)

                                                                      gCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTg                                                                              >  2:1447800/1‑150 (MQ=255)

                                                                      gCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTg                                                                              <  1:1656926/150‑1 (MQ=255)

                                                                       cTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTgg                                                                             <  2:4224900/150‑1 (MQ=255)

                                                                       cTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTgg                                                                             <  2:4302789/150‑1 (MQ=255)

                                                                       cTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTgg                                                                             <  2:4224826/150‑1 (MQ=255)

                                                                       cTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTgg                                                                             <  1:4914488/150‑1 (MQ=255)

                                                                        tgtgGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTCCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGc                                                                            >  2:1105777/1‑150 (MQ=255)

                                                                           ggCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTAc                                                                         <  2:1923065/150‑1 (MQ=255)

                                                                           ggCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTAc                                                                         <  2:1181980/150‑1 (MQ=255)

                                                                           ggCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTAc                                                                         <  1:597973/150‑1 (MQ=255)

                                                                             cATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACAt                                                                       <  1:7538629/150‑1 (MQ=255)

                                                                                 ggCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCtat                                                                   <  1:692894/150‑1 (MQ=255)

                                                                                 ggCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCtat                                                                   <  2:6290788/150‑1 (MQ=255)

                                                                                     gcgAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTg                                                               <  2:2447884/150‑1 (MQ=255)

                                                                                       gAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGcc                                                             <  2:6484062/150‑1 (MQ=255)

                                                                                         agagTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCa                                                           <  2:6335927/150‑1 (MQ=255)

                                                                                             tACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAgccg                                                       >  2:6495101/1‑150 (MQ=255)

                                                                                                aatcctgACAAGCTGCGCTACCATAAGATCATCATCATGACGGATGCTGACGTCGACGGTGCGCATATTCGTACGCTGCTGTTGACCTTCTTCTTCCGTCAGATGCCGGAACTGATCGAGCGTGGCCATATCTATATCGCGCAGccgccg                                                    <  2:8056282/144‑1 (MQ=255)

                                                                                                aacaTCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGccgccg                                                    >  1:3815789/1‑150 (MQ=255)

                                                                                                 acaTCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCcgccgc                                                   <  1:7554178/150‑1 (MQ=255)

                                                                                                  tcctgACAAGCTGCGCTACCATAAGATCATCATCATGACGGATGCTGACGTCGACGGTGCGCATATTCGTACGCTGCTGTTGACCTTCTTCTTCCGTCAGATGCCGGAACTGATCGAGCGTGGCCATATCTATATCGCGCAGCCGCCGCt                                                  >  1:2850627/5‑150 (MQ=255)

                                                                                                  tcctgACAAGCTGCGCTACCATAAGATCATCATCATGACGGATGCTGACGTCGACGGTGCGCATATTCGTACGCTGCTGTTGACCTTCTTCTTCCGTCAGATGCCGGAACTGATCGAGCGTGGCCATATCTATATCGCGCAGCCGCCGCt                                                  >  2:4986176/5‑150 (MQ=255)

                                                                                                   cctgACAAGCTGCGCTACCATAAGATCATCATCATGACGGATGCTGACGTCGACGGTGCGCATATTCGTACGCTGCTGTTGACCTTCTTCTTCCGTCAGATGCCGGAACTGATCGAGCGTGGCCATATCTATATCGCGCAGCCGCCGCtc                                                 <  2:3218730/147‑2 (MQ=255)

                                                                                                   aTCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTg                                                 >  2:3142616/1‑150 (MQ=255)

                                                                                                    tCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGt                                                >  2:1967393/1‑150 (MQ=255)

                                                                                                     cGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTa                                               >  1:3175958/1‑150 (MQ=255)

                                                                                                      gACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTaa                                              >  1:6411947/1‑149 (MQ=255)

                                                                                                      gACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTAc                                              <  1:2856331/150‑1 (MQ=255)

                                                                                                        cAAGCTGCGCTACCATAAGATCATCATCATGACGGATGCTGACGTCGACGGTGCGCATATTCGTACGCTGCTGTTGACCTTCTTCTTCCGTCAGATGCCGGAACTGATCGAGCGTGGCCATATCTATATCGCGCAGCCGCCGCTCTATaa                                            <  1:7948455/150‑1 (MQ=255)

                                                                                                        cAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACaa                                            <  1:144965/150‑1 (MQ=255)

                                                                                                        cAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTACTTCGTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACaa                                            <  1:3361349/150‑1 (MQ=255)

                                                                                                          aaCTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAgg                                          <  2:8723668/150‑1 (MQ=255)

                                                                                                             tGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGa                                       >  2:7213606/1‑150 (MQ=255)

                                                                                                              gCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGaa                                      >  2:3470967/1‑150 (MQ=255)

                                                                                                                gTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAAc                                    >  1:6389589/1‑150 (MQ=255)

                                                                                                                gTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAAc                                    <  2:5566960/150‑1 (MQ=255)

                                                                                                                  tATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGccgccg                                                    <  1:3697209/132‑1 (MQ=255)

                                                                                                                  tATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGa                                  >  2:5928160/1‑150 (MQ=255)

                                                                                                                   aTCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTTTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAg                                 <  1:1883845/150‑1 (MQ=255)

                                                                                                                   aTCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAg                                 <  1:5332642/150‑1 (MQ=255)

                                                                                                                     cacaACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGc                               <  1:1715664/150‑1 (MQ=255)

                                                                                                                     cacaACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGc                               <  1:5102739/150‑1 (MQ=255)

                                                                                                                      acaacaTCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGTGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACCAGGTGAAACGAGGCa                              >  2:8302251/1‑150 (MQ=255)

                                                                                                                      acaacaTCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCa                              >  2:183463/1‑150 (MQ=255)

                                                                                                                      acaacaTCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCa                              >  2:5309581/1‑150 (MQ=255)

                                                                                                                      acaacaTCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCa                              >  2:8522073/1‑150 (MQ=255)

                                                                                                                       caacaTCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCaa                             <  2:8614852/150‑1 (MQ=255)

                                                                                                                        aacaTCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAg                            >  1:4359938/1‑150 (MQ=255)

                                                                                                                        aacaTCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAg                            >  1:8636946/1‑150 (MQ=255)

                                                                                                                         acaTCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGc                           >  1:6810256/1‑150 (MQ=255)

                                                                                                                          catcatcatcatGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCa                          >  2:7826326/1‑150 (MQ=255)

                                                                                                                             catcatcatGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGa                       <  2:407007/150‑1 (MQ=255)

                                                                                                                                catcatGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCa                    <  1:6200877/150‑1 (MQ=255)

                                                                                                                                    atGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGccc                                                            <  2:1571445/106‑1 (MQ=255)

                                                                                                                                       aCCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATc             >  2:5542899/1‑150 (MQ=255)

                                                                                                                                        ccGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCa            <  2:3928035/150‑1 (MQ=255)

                                                                                                                                               tGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGa     >  1:2809312/1‑150 (MQ=255)

                                                                                                                                                gACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGAt    >  2:5351613/1‑150 (MQ=255)

                                                                                                                                                 aCGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATg   >  2:6901979/1‑150 (MQ=255)

                                                                                                                                                  cGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGa  <  2:4348980/150‑1 (MQ=255)

                                                                                                                                                  cGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGa  <  2:3670686/150‑1 (MQ=255)

AACGTCGAGAAAGCGCGCTTCGACAAGATGATTTCGTCCCAGGAAGTGGGCACGCTGATCACTGCGCTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGA  >  NC_002947/14689‑14984

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`