Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,392,942 T→C 12.7% intergenic (+106/‑80) PP_1214 → / → ruvC transcriptional regulator/crossover junction endodeoxyribonuclease RuvC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,392,9420TC12.7% 523.9 / 51.6 198intergenic (+106/‑80)PP_1214/ruvCtranscriptional regulator/crossover junction endodeoxyribonuclease RuvC
Reads supporting (aligned to +/- strand):  ref base T (95/74);  new base C (9/16);  total (108/90)
Fisher's exact test for biased strand distribution p-value = 8.44e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.06e-02

Only 100 of 205 total aligned reads displayed.

CCAATGCCCAGATTTCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTAC‑GCC‑CA‑TTTCAAATGTGGTGCAGTACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCACCGGCTATGGCGTGGTACGCCAGACCGCCC  >  NC_002947/1392793‑1393091
                                                                                                                                                        |                                                                                                                                                     
ccAATGCCCAGATTTCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTAC‑GCC‑CA‑TTTCAAATgtggt                                                                                                                                                       <  1:4764356/150‑1 (MQ=255)
 cAATGCCCAGATTTCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTAC‑GCC‑CA‑TTTCAAATgtggtg                                                                                                                                                      <  2:5975321/150‑1 (MQ=255)
  aaTGCCCAGATTTCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTAC‑GCC‑CA‑TTTCAAATGTGGTGc                                                                                                                                                     <  1:8790341/150‑1 (MQ=255)
  aaTGCCCAGATTTCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTAC‑GCC‑CA‑TTTCAAATGTGGTGc                                                                                                                                                     <  2:8846411/150‑1 (MQ=255)
   aTGCCCAGATTTCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTAC‑GCC‑CA‑TTTCAAATGTGGTGCa                                                                                                                                                    <  2:4485229/150‑1 (MQ=255)
   aTGCCCAGATTTCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTAC‑GCC‑CA‑TTTCAAATGTGGTGCa                                                                                                                                                    <  2:3713488/150‑1 (MQ=255)
        cAGATTTCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCATTTCGCGGGTAAACCCGCTCCTACAGGTATTAC‑GCC‑CA‑TTTCAAATGTGGTGCAGTAcc                                                                                                                                               <  2:373847/150‑1 (MQ=255)
           aTTTCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTACTGC‑ACCACA‑TTTGAAATG‑GGCGTAATACCTGt                                                                                                                                            <  2:6470613/150‑1 (MQ=21)
            tttCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTACTGC‑ACCACA‑TTTGAAATG‑GGCGTAATACCTGTa                                                                                                                                           <  1:5464451/150‑1 (MQ=21)
            tttCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGCGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTACTGC‑ACCACA‑TTTGAAATG‑GGCGTAATACCTGTa                                                                                                                                           <  1:5295225/150‑1 (MQ=18)
             ttCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTAC‑GCC‑CA‑TTTCAAATGTGGTGCAGTACCTGTAg                                                                                                                                          <  1:2615634/150‑1 (MQ=255)
             ttCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTAC‑GCC‑CA‑TTTCAAATGTGGTGCAGTACCTGTAg                                                                                                                                          <  2:6805808/150‑1 (MQ=255)
             ttCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTACTGC‑ACCACA‑TTTGAAATG‑GGCGTAATACCTGTAg                                                                                                                                          <  2:2554448/150‑1 (MQ=18)
              tCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTAC‑GCC‑CA‑TTTCAAATGTGGTGCAGTACCTGTAgg                                                                                                                                         <  1:6254083/150‑1 (MQ=255)
               ccGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACCGGTATTAC‑GCC‑CA‑TTTCAAATGTGGTGCAGTACCTGTAGGa                                                                                                                                        <  1:4503731/150‑1 (MQ=255)
                 gacgaGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTACTGC‑ACCACA‑TTTGAAATG‑GGCGTAATACCTGTAGGAGc                                                                                                                                      <  1:2944319/150‑1 (MQ=18)
                   cgaGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTAC‑GCC‑CA‑TTTCAAATGTGGTGCAGTACCTGTAGGAGCgg                                                                                                                                    <  1:1703740/150‑1 (MQ=255)
                   cgaGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTAC‑GCC‑CA‑TTTCAAATGTGGTGCAGTACCTGTAGGAGCgg                                                                                                                                    <  1:767109/150‑1 (MQ=255)
                   cgaGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTAC‑GCC‑CA‑TTTCAAATGTGGTGCAGTACCTGTAGGAGCgg                                                                                                                                    <  1:4656171/150‑1 (MQ=255)
                    gagaTCATGGAAAACCTCGGCTAAGGTGATTTGGCTTTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTACTGC‑ACCACA‑TTTGAAATG‑GGCGTAATACCTGTAGGAGCggg                                                                                                                                   <  1:3869268/150‑1 (MQ=18)
                    gagaTCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTAC‑GCC‑CA‑TTTCAAATGTGGTGCAGTACCTGTAGGAGCggg                                                                                                                                   <  1:725460/150‑1 (MQ=255)
                    gagaTCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTAC‑GCC‑CA‑TTTCAAATGTGGTGCAGTACCTGTAGGAGCggg                                                                                                                                   <  1:3703052/150‑1 (MQ=255)
                    gagaTCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTAC‑GCC‑CA‑TTTCAAATGTGGTGCAGTACCTGTAGGAGCggg                                                                                                                                   <  1:6880914/150‑1 (MQ=255)
                      gaTCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTAC‑GCC‑CA‑TTTCAAATGTGGTGCAGTACCTGTAGGAGCGGGtt                                                                                                                                 <  1:4036150/150‑1 (MQ=255)
                      gaTCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTAC‑GCC‑CA‑GTTCAAATGTGGTGCAGTACCTGGAGGAGCGGGtt                                                                                                                                 >  2:9023992/1‑150 (MQ=255)
                         cATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTAC‑GCC‑CA‑TTTCAAATGTGGTGCAGTACCTGTAGGAGCGGGTTTAc                                                                                                                              <  2:7821861/150‑1 (MQ=255)
                         cATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTAC‑GCC‑CA‑TTTCAAATGTGGTGCAGTACCTGTAGGAGCGGGTTTAc                                                                                                                              <  1:7596504/150‑1 (MQ=255)
                          aTGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTTAACCCGCGCCTACCGGTATTAC‑GCC‑CC‑ATTCAAAAGTGGTGGAGGACCAGGAGGAGCGGGGTTTcc                                                                                                                             >  2:211680/1‑150 (MQ=255)
                          aTGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTGAAACCGCTCCTACAGGTATTAC‑GCC‑CA‑AATGAAATGTTGTGCAGTACCAGTGGGAGCGGGGTTagc                                                                                                                             >  1:6794463/1‑148 (MQ=38)
                          aTGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTAC‑GCC‑CA‑TTTCAAATGTGGTGCAGTACCTGTAGGAGCGGGTTTAcc                                                                                                                             <  2:5004989/150‑1 (MQ=255)
                          aTGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTAC‑GCC‑CA‑TTTCAAATGTGGTGCAGTACCTGTAGGAGCGGGTTTAcc                                                                                                                             <  2:544691/150‑1 (MQ=255)
                          aTGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTAC‑GCC‑CA‑TTTCAAATGTGGTGCAGTACCTGTAGGAGCGGGTTTAcc                                                                                                                             <  2:7243873/150‑1 (MQ=255)
                          aTGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCGTTCGCGGGGAAAACCGCGCCAAAAGGTATTAC‑GCC‑CC‑ATTGAAAAGTGGTGCCGTAACGGGTGGGGCGGGGTTagc                                                                                                                             >  1:6794609/1‑148 (MQ=255)
                             gAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTAC‑GCC‑CA‑TTTCAAATGTGGTGCAGTACCTGTAGGAGCGGGTTTACCcgc                                                                                                                          <  2:4709773/150‑1 (MQ=255)
                                   cTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGGAAACCCCCGGCTAAAGGTGTTAG‑GCC‑CC‑TTTCAAATGTGGTGCGGGACCGGGTGGGGGGGGGTTAGCCGCCGAAgg                                                                                                                    >  2:7709820/1‑150 (MQ=255)
                                      ggCTATGGTGTTTTGGCATTTGGTGCCAACTCTGAGGGTTTGATAGCGCCTGTGCGGGCCCTTTCGGGGTTAAACCCGCTCCTACAGGTATTAC‑GCC‑CA‑TTTCAAATGTGGTGCAGTACCTGTAGGAGGGGGTTTACCCGCGAAAGGGcc                                                                                                                 <  2:5773311/150‑1 (MQ=37)
                                       gatacggTTATTTGGCTTTTTGTGCCAACTCTGAGCTTTTGATCGCGCCTGTGCGGGCCCTTTCGCGGGTAACCCCGCTCCTACAGGTTTTAC‑GCC‑AA‑TTTCAAATGTGGTGAAGTACCTTTAGGAGCGGTTTTACCCGCGAAAGGGCCg                                                                                                                <  2:4796485/145‑1 (MQ=25)
                                       gCTTAGGTGTTTTGGCTTTTTGTGCCAACTCTGAGCGTTTGTTAGCGCCTGTGCCGGCCCTTTCGCGGTTAAACCCGCTCCTACCGGTATTAC‑GCC‑CA‑TTTCAAATGTGGTGCAGTACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCg                                                                                                                <  2:4795251/150‑1 (MQ=37)
                                         tAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAAACCGCTCCAACAGGTATTAA‑GCC‑CA‑TTTCAAATGTGGTGCAGTAACTGGAGGATCGGGGTTAACCGCGAAAGGGCCCgc                                                                                                              >  2:4484435/1‑150 (MQ=38)
                                             gTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCGTTCGCGGGGAAACCCCCGCCGACCCGGATTAC‑GCC‑CA‑AGTCAAATGGGGTGCCGTACCGGTAGGAGCGGGGTTACCCCCGCCAGGGCCCgccccg                                                                                                          >  1:6056075/1‑146 (MQ=255)
                                                             tgCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAAACCGCGCCGACAGGTATTAC‑GCC‑CA‑TTTCAAATGTGGTGCAGTACCCGGAGGAGCGGGTTTTCCCGCGAAAGGGCCGGCGCCGTTTGTAGAGATGcatc                                                                                          >  2:4400798/1‑150 (MQ=37)
                                                                cAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCACCTACAGGTATTAC‑GCC‑CA‑TTTAAAAAGTGGTGCAGTACCAGTAGGAGCCGGTTTACCCCCGGAAGGGCCGGCGCAGGTTGTAGAGATGCATCCtt                                                                                       >  2:3690749/1‑150 (MQ=37)
                                                                cAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTAGCGGGTAAAACCCCGCCAAAACGTATTAA‑GCC‑CA‑ATTAAAAATTGGTGCCGTACCCGTGGGAGCGGGGTTACCCGCGCAAGGGCCGGCGCCGTGTGTGGTGCTTCATCAtt                                                                                       >  2:163042/1‑150 (MQ=255)
                                                                 aaCTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAAACCCCTCCAACAGGTATTAC‑GCC‑CA‑ATTCAAATGTGGTGCAGTACCGGTAGGAGCGGGTTTAACCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCAAGAttt                                                                                      >  2:7358814/1‑150 (MQ=37)
                                                                  aCTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAAACCCCTACTAAAGGTATTAA‑GCC‑CA‑ATTAAAATGTTGTGCAGTACCAGTAGGAGCGGGTTTACCCCCGCAAGGGCCCGCGCAGTTTGTATATATGCAAAAtttt                                                                                     >  2:4324281/1‑150 (MQ=255)
                                                                            ttttATCGCGCCTGGGCGGGCCCTTTCGCGGTTAAACCCGCCCCTACCGTTATAAC‑GCC‑CA‑TTTCAAATGTGGTGCATTACCTGTTGGCGCGGTTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGc                                                                           <  2:2887820/150‑1 (MQ=25)
                                                                            tttGATTGCGCGTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGTTATTAC‑GCC‑CA‑TTTAAATTGTGGTGCTTTACCTTTAGGCGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGc                                                                           <  2:2888211/150‑1 (MQ=37)
                                                                            tttGATAGCGCCTGTGCCGGCCCTTTCGCGGGGAAAACCCCGCCGACAGGTAATAC‑GCC‑CA‑ATTGAAATGTGGTGGAGGAACAGTAGGAGCGGGTTTACCCGCGACAGGGCCGGCGCAGGTTGTTGGGGTGCATCATTTTTTAGACgggg                                                                           >  2:3421700/1‑149 (MQ=21)
                                                                                aTAGCGCCTGTGCCGGCCCTTTCGCGGGTAAAACCCCACCAAAACGGATTAA‑GCC‑CC‑AATCAAATTGGGGGCCGTAGCCGTCGGGGCGGGGTTAACCCCCCAAGGGGCCGCGCCGGTTGTATAGATGCCACATTTTTTAGAAGGGCTTTa                                                                       >  2:4242223/1‑150 (MQ=255)
                                                                                 tggcgcGTGTGCGGGCCGTTGCG‑GGGTTAACCCGGCCCCGCCGGTTTTACGGCC‑CT‑TTTATATTGTGGTGCCTTACGTGTGGGTGGGGTTTTCCCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTAt                                                                      <  2:4167978/148‑1 (MQ=255)
                                                                                   gcggCTGTGCCGCCCCTTTCGCGGTTAACCCCGATCCCACCGGTCTCAC‑CCC‑CA‑TTTCACATGTGGTGCAGTACCTGTAGGTGGGGTTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGa                                                                    <  2:8768650/150‑1 (MQ=37)
                                                                                    cgcCTGTGCCGGCCCTTTCGCGGGTAAAACCCCTCCAACAGGGATTAC‑GCC‑CA‑TTTCAAAAGTGGGGCAGTGCCGGTAGGAGCGGGTTTACCCGCGCAAGGGCCGGCGCCGGTTGTAGAGCTGCATCATTTTTTCGACGGGCTTTAttaa                                                                   >  2:4515849/1‑147 (MQ=25)
                                                                                      ccTGTGCCGGCCCTTTCGCGGGTAAACCCCCTCCTACAGGTATTAA‑GCC‑CC‑TTTGAAATGGGGGGCAGTAACGGTAGGAGCGGGGTTAACCGCGAAAGGGCCCGCGCCGGTTGTAGAGAAGCCTCAATTTTTAGACGGGGGTTATGActc                                                                 >  2:8121291/1‑150 (MQ=18)
                                                                                              ggCCCTTGCGCGGGTAACCCCGCTCCTACAGGTATTAC‑GCC‑CA‑TTTCAATTGTGGTGCCGTACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTa                                                         <  2:8970281/150‑1 (MQ=255)
                                                                                              gccccTTCCGGGGTTAACCCCGCCCCTACCGTTA‑TAC‑CCC‑CATTTTCAATGGTGGTGCTGTACGTTTTGGCGGGGTTTTACCCGCGAACGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTa                                                         <  2:3991863/148‑1 (MQ=255)
                                                                                               gCCCTTTCGGGGTTAAACCCGCCCCTTCAGTTATTAC‑GCC‑AT‑TTTCAACTGTGGTTCAGTACCTGTTGGAGCGGGTTTACCCGCGAATGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAg                                                        <  2:5833729/150‑1 (MQ=38)
                                                                                               gCCCTTCGGCGGTAAACCCCGCTCCTACCGTTATTCC‑CCC‑CA‑TTTCAACGGTGGTGCTGAACCTGTTGGCGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAg                                                        <  1:8362381/150‑1 (MQ=255)
                                                                                                 ccTTTAGCGGGTAAAACCGCACCAAAAGGTAATAA‑GCC‑CA‑TTTCAAATGTGGTGCAGTAACTGTAGGAGCCGGTTTAACCCCGAAAGGGCCGGCGCAGTTTGTAGAGATTCATCATTTTTTCGACGGGCGTTAAGACAATGTTTCGAGGt                                                      >  2:3786890/1‑150 (MQ=255)
                                                                                                       gcgGGTAAACCCGCTCCTACAGGTATTAC‑GCC‑CA‑TTTCAAATGTGGTGCAGTACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGAc                                                >  2:6891310/1‑150 (MQ=255)
                                                                                                         gggTAAACCCGCTCCTACAGGTACTGC‑ACCACA‑TTTGAAATG‑GGCGTAATACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGAccc                                              >  1:3357009/1‑150 (MQ=17)
                                                                                                               aCCCGCTCCTACAGGTATTAC‑GCC‑CA‑TTTCAAATGTGGTGCAGTACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTc                                        >  2:6106145/1‑150 (MQ=255)
                                                                                                                cccGCTCCTACAGGTATTAC‑GCC‑CA‑TTTCAAATGTGGTGCAGTACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTcg                                       >  2:6366017/1‑150 (MQ=255)
                                                                                                                 ccGCTCCTACAGGTATTAC‑GCC‑CC‑TTTCAAATGTGGTGCAGTACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTCGCGCTGCATCATTTTTTCGACGGGCTGTATGACTCTGATTCTAGGTATCGACCCCGGCTcgc                                      >  2:7003043/1‑150 (MQ=38)
                                                                                                                 ccGCTCCTACAGGTATTAC‑GCC‑CA‑TTTCAAATGTGGTGCAGTACCTGTAGGAGCGGGTTTACCCGCGACAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTcgc                                      >  1:3020454/1‑150 (MQ=255)
                                                                                                                 ccGCTCCTACAGGTATTAC‑GCC‑CA‑TTTCAAATGTGGTGCAGTACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTcgc                                      >  1:2553293/1‑150 (MQ=255)
                                                                                                                 ccGCTCCTACAGGTATTAC‑GCC‑CA‑TTTCAAATGTGGTGCAGTACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTcgc                                      >  2:7186247/1‑150 (MQ=255)
                                                                                                                 ccGCTCCTACAGGTATTAC‑GCC‑CA‑TTTCAAATGTGGTGCAGTACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTcgc                                      >  1:6407426/1‑150 (MQ=255)
                                                                                                                 ccGCTCCTACAGGTACTGC‑ACCACA‑TTTGAAATG‑GGCGTAATACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTcgc                                      >  2:3534747/1‑150 (MQ=18)
                                                                                                                  cGCTCCTACAGGTCTTAC‑GCC‑CA‑TTTCAAATGTGGTGCAGTACCTGTAGGAGCGGGTTTACCCGCGACAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTcgcg                                     >  2:6302244/1‑150 (MQ=255)
                                                                                                                     tCCTACAGGTACTGC‑ACCACA‑TTTGAAATG‑GGCGTAATACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCACAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCAt                                  >  1:6184101/1‑150 (MQ=18)
                                                                                                                       cTACAGGTACTGC‑ACCACA‑TTTGAAATG‑GGCGTAATACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTAt                                                                      >  1:1307246/1‑112 (MQ=11)
                                                                                                                        tACAGGTATTAC‑GCC‑CA‑TTTCAAATGTGGTGCAGTACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCAc                               >  2:5127937/1‑150 (MQ=255)
                                                                                                                        tACAGGTATTAC‑GCC‑CA‑TTTCAAATGTGGTGCAGTACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCAc                               >  1:4570464/1‑150 (MQ=255)
                                                                                                                        tACAGGTATTAC‑GCC‑CA‑TTTCAAATGTGGTGCAGTACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCAc                               >  1:735331/1‑150 (MQ=255)
                                                                                                                        tACAGGTATTAC‑GCC‑CA‑TTTCAAATGTGGTGCAGTACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCAGCAc                               >  2:5203434/1‑150 (MQ=255)
                                                                                                                        tACAGGTATTAC‑GCC‑CA‑TTTCAAATGTGGTGCAGTACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATGCAAGGTATCGACCCCGGCTCGCGCATCAc                               >  1:2230271/1‑150 (MQ=255)
                                                                                                                        tACAGGTACTGC‑ACCACA‑TTTGAAATG‑GGCGTAATACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCAc                               >  1:7886107/1‑150 (MQ=21)
                                                                                                                         aCAGGTATTAC‑GCC‑CA‑TTTCAAATGTGGTGCAGTACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCAcc                              >  2:3840291/1‑150 (MQ=255)
                                                                                                                         aCAGGTATTAC‑GCC‑CA‑TTTCAAATGTGGTGCAGTACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCAcc                              >  1:7359738/1‑150 (MQ=255)
                                                                                                                           aGGTATTAC‑GCC‑CA‑TTTCAAATGTGGTGCAGTACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCACCgg                            >  2:5618554/1‑150 (MQ=255)
                                                                                                                           aGGTATTAC‑GCC‑CA‑TTTCAAATGTGGTGCAGTACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCACCgg                            >  2:1069864/1‑150 (MQ=255)
                                                                                                                             gTATTAC‑GCC‑CA‑TTTCAAATGTGGTGCAGTACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCACCGGCt                          >  1:1105/1‑150 (MQ=255)
                                                                                                                                  tgcaccacA‑TTTGAAATG‑GGCGTAATACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCACAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCACCGGCTATgg                      >  1:7466871/8‑150 (MQ=25)
                                                                                                                                  aC‑GCC‑CT‑TTTCAATTGTGGTGATGTACCGGTTGGAGGGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCACCGGCTATGGc                     <  2:670963/150‑1 (MQ=255)
                                                                                                                                     gCC‑CA‑TTTCAAATGTGGTGCAGTACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTa                                                         <  1:838999/112‑1 (MQ=255)
                                                                                                                                     gCC‑CA‑TTTCAAATGTGGTGCAGTACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTa                                                         >  2:838999/1‑112 (MQ=255)
                                                                                                                                     gCC‑CA‑TTTCAAATGTGGTGCAGTACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCACCGGCTATGGCGt                   >  1:6900673/1‑150 (MQ=255)
                                                                                                                                      cc‑cA‑TTTCAAATGTGGTGCAGTACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCACCGGCTATGGCGTg                  <  1:1069806/150‑1 (MQ=255)
                                                                                                                                       c‑cA‑TTTCAAATGTGGTGCAGTACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCACCGGCTATGGCGTgg                 >  2:8073981/1‑150 (MQ=255)
                                                                                                                                       c‑cA‑TTTCAAATGTGGTGCAGTACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCACCGGCTATGGCGTgg                 >  1:8555458/1‑150 (MQ=255)
                                                                                                                                       c‑cA‑TTTCAAATGTGGTGCAGTACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTGTGTCGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCACCGGCTATGGCgggg                 >  1:8959432/1‑150 (MQ=255)
                                                                                                                                         cA‑TTTCAAATGTGGTGCAGTACCTGTAGGAGCGGGTTTAACCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATAGACACCGGCTCGCGCATCACCGGCTATGGCGTGGt                >  2:8152749/1‑150 (MQ=255)
                                                                                                                                            tttCAAATGTGGTGCAGTACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCACCGGCTATGGCGTGGTAc              >  2:8035081/1‑150 (MQ=255)
                                                                                                                                            tttCAAATGTGGTGCAGTACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCACCGGCTATGGCGTGGTAc              >  2:5277580/1‑150 (MQ=255)
                                                                                                                                             ttCAAATGTGGTGCAGTACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCACCGGCTATGGCGTGGTACg             >  2:5111542/1‑150 (MQ=255)
                                                                                                                                                aaaTGTGGTGCAGTACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCACCGGCTATGGCGTGGTACGCCa          >  2:8376434/1‑150 (MQ=255)
                                                                                                                                                aaaTGTGGTGCAGTACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCACCGGCTATGGCGTGGTACGCCa          >  1:349649/1‑150 (MQ=255)
                                                                                                                                                aaaTGTGGTGCAGTACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCACCGGCTATGGAGTGGTACGCCa          >  2:1941807/1‑150 (MQ=255)
                                                                                                                                                        tgCAGTACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCACCGGCTATGGCGTGGTACGCCAGACCGccc  >  1:5152898/1‑150 (MQ=255)
                                                                                                                                                        tgCAGTACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCACCGGCTATGGCGTGGTACGCCAGACCGccc  >  1:8266781/1‑150 (MQ=255)
                                                                                                                                                        |                                                                                                                                                     
CCAATGCCCAGATTTCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTAC‑GCC‑CA‑TTTCAAATGTGGTGCAGTACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCACCGGCTATGGCGTGGTACGCCAGACCGCCC  >  NC_002947/1392793‑1393091

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑