Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,464,198 T→G 9.3% Q476P (CAG→CCG)  algX ← alginate biosynthesis protein AlgX

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,464,1980TG 9.3% 503.5 / 3.9 216Q476P (CAG→CCG) algXalginate biosynthesis protein AlgX
Reads supporting (aligned to +/- strand):  ref base T (107/79);  new base G (3/23);  total (114/102)
Fisher's exact test for biased strand distribution p-value = 1.02e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 231 total aligned reads displayed.

TCACCGGTCTTGAGCTTTTCGATCCCCTGGTAGTAACCCTGCGGCGGTACCAGCGCGGCGTGCGCGGCACCACCAAACAAGGCCAACGCGAGCAGGGCGGGGCTGGAAATTCGTTTGAAAAGGGTCA‑TGGGTCGCCTCACTGGCC‑TGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGTACCTCGAACGCGAGCAGGCGCTGGCTGGCCCAGTCCTCGTCTTCGCGCATCTGGAAGACGAAGCGG  >  NC_002947/1464049‑1464347
                                                                                                                                                       |                                                                                                                                                     
tCACCGGTCTTGAGCTTTTCGATCCCCTGGTAGTAACCCTGCGGCGGTACCAGCGCGGCGTGCGCGGCACCACCAAACAAGGCCAACGCGAGCAGGGCGGGGCTGGAAATTCGTTTGAAAAGGGTCA‑TGGGTCGCCTCACTGgcc‑tgcct                                                                                                                                                       >  1:8472331/1‑150 (MQ=255)
     ggTCTTGAGCTTTTCGATCCCCTGGTAGTAACCCTGCGGCGGTACCAGCGCGGCGTGCGCGGCACCACCAAACAAGGCCAACGCGAGCAGGGCGGGGCTGGAAATTCGTTTGAAAAGGGTCA‑TGGGTCGCCTCACTGGCC‑TGCCTGcgcg                                                                                                                                                  >  1:766433/1‑150 (MQ=255)
     ggTCTTGAGCTTTTCGATCCCCTGGTAGTAACCCTGCGGCGGTACCAGCGCGGCGTGCGCGGCACCACCAAACAAGGCCAACGCGAGCAGGGCGGGGCTGGAAATTCGTTTGAAAAGGGTCA‑TGGGTCGCCTCACTGGCC‑TGCCGGcgcg                                                                                                                                                  >  1:4687302/1‑150 (MQ=255)
         ttGAGCTTTTCGATCCCCTGGTAGTAACCCTGCGGCGGTACCAGCGCGGCGTGCGCGGCACCACCAAACAAGGCCAACGCGAGCAGGGCGGGGCTGGAAATTCGTTTGAAAAGGGTCA‑TGGGTCGCCTCACTGGCC‑TGCCTGCGCGGTcg                                                                                                                                              >  1:7237819/1‑149 (MQ=255)
         ttGAGCTTTTCGATCCCCTGGTAGTAACCCTGCGGCGGTACCAGCGCGGCGTGCGCGGCACCACCAAACAAGGCCAACGCGAGCAGGGCGGGGCTGGAAATTCGTTTGAAAAGGGTCA‑TGGGTCGCCTCACTGGCC‑TGCCTGCGCGGTCt                                                                                                                                              >  2:1666052/1‑150 (MQ=255)
           gAGCTTTTCGATCCCCTGGTAGTAACCCTGCGGCGGTACCAGCGCGGCGTGCGCGGCACCACCAAACAAGGCCAACGCGAGCAGGGCGGGGCTGGAAATTCGTTTGAAAAGGGTCA‑TGGGTCGCCTCACTGGCC‑TGCCTGCGCGGTCTgc                                                                                                                                            >  1:5005634/1‑150 (MQ=255)
            aGCTTTTCGATCCCCTGGTAGTAACCCTGCGGCGGTACCAGCGCGGCGTGCGCGGCACCACCAAACAAGGCCAACGCGAGCAGGGCGGGGCTGGAAATTCGTTTGAAAAGGGTCA‑TGGGTCGCCTCACTGGCC‑TGCCTGCGCGGTCTgcg                                                                                                                                           >  1:6651796/1‑150 (MQ=255)
             gCTTTTCGATCCCCTGGTAGTAACCCTGCGGCGGTACCAGCGCGGCGTGCGCGGCACCACCAAACAAGGCCAACGCGAGCAGGGCGGGGCTGGAAATTCGTTTGAAAAGGGTCA‑TGGGTCGCCTCACTGGCC‑TGCCTGCGCGGTCTgcgc                                                                                                                                          >  1:3203254/1‑150 (MQ=255)
             gCTTTTCGATCCCCTGGTAGTAACCCTGCGGCGGTACCAGCGCGGCGTGCGCGGCACCACCAAACAAGGCCAACGCGAGCAGGGCGGGGCTGGAAATTCGTTTGAAAAGGGTCA‑TGGGTCGCCTCACTGGCC‑TGCCTGCGCGGGCTgcgc                                                                                                                                          >  1:5589380/1‑150 (MQ=255)
               ttttCGATCCCCTGGTAGTAACCCTGCGGCGGTACCAGCGCGGCGTGCGCGGCACCACCAAACAAGGCCAACGCGAGCAGGGCGGGGCTGGAAATTCGTTTGAAAAGGGTCA‑TGGGTCGCCTCACTGGCC‑TGCCTGCGCGGTCTGCGCAg                                                                                                                                        >  2:5513867/1‑150 (MQ=255)
               ttttCGATCCCCTGGTAGTAACCCTGCGGCGGTACCAGCGCGGCGTGCGCGGCACCACCAAACAAGGCCAACGCGAGCAGGGCGGGGCTGGAAATTCGTTTGAAAAGGGTCA‑TGGGTCGCCTCACTGGCC‑TGCCTGCGCGGTCTGCGCAg                                                                                                                                        >  1:6322516/1‑150 (MQ=255)
               ttttCGATCCCCTGGTAGTAACCCTGCGGCGGTACCAGCGCGGCGTGCGCGGCACCACCAAACAAGGCCAACGCGAGCAGGGCGGGGCTGGAAATTCGTTTGAAAAGGGTCA‑TGGGTCGCCTCACTGGCC‑TGCCTGCGCGGTCTGCGCAg                                                                                                                                        >  1:2598887/1‑150 (MQ=255)
                tttCGATCCCCTGGTAGTAACCCTGCGGCGGTACCAGCGCGGCGTGCGCGGCACCACCAAACAAGGCCAACGCGAGCAGGGCGGGGCTGGAAATTCGTTTGAAAAGGGTCA‑TGGGTCGCCTCACTGGCC‑TGCCTGCGCAGACCGCGCAgg                                                                                                                                       <  1:100779/150‑1 (MQ=255)
                  tCGATCCCCTGGTAGTAACCCTGCGGCGGTACCAGCGCGGCGTGCGCGGCACCACCAAACAAGGCCAACGCGAGCAGGGCGGGGCTGGAAATTCGTTTGAAAAGGGTCA‑TGGGTCGCCTCACTGGCC‑TGCCTGCGCGGTCTGCGCAGGCa                                                                                                                                     <  2:137388/150‑1 (MQ=255)
                   cGATCCCCTGGTAGTAACCCTGCGGCGGTACCAGCGCGGCGTGCGCGGCACCACCAAACAAGGCCAACGCGAGCAGGGCGGGGCTGGAAATTCGTTTGAAAAGGGTCA‑TGGGTCGCCTCACTGGCC‑TGCCTGCGCGGTCTGCGCAGGCAc                                                                                                                                    <  1:6003289/150‑1 (MQ=255)
                   cGATCCCCTGGTAGTAACCCTGCGGCGGTACCAGCGCGGCGTGCGCGGCACCACCAAACAAGGCCAACGCGAGCAGGGCGGGGCTGGAAATTCGTTTGAAAAGGGTCA‑TGGGTCGCCTCACTGGCC‑TGCCTGCGCGGTCTGCGCAGGCAc                                                                                                                                    <  2:3663813/150‑1 (MQ=255)
                    gATCCCCTGGTAGTAACCCTGCGGCGGTACCAGCGCGGCGTGCGCGGCACCACCAAACAAGGCCAACGCGAGCAGGGCGGGGCTGGAAATTCGTTTGAAAAGGGTCA‑TGGGTCGCCTCACTGGCCGTGCCTGCGCAGACCGCGCAGGCag                                                                                                                                    <  1:1245123/150‑2 (MQ=255)
                     aTCCCCTGTTAGTAACCCTGCGGCGGTACCAGCGCGGCGTGCGCGGCACCACCAAACAAGGCCAACGCGAGCAGGGCGGGGCTGGAAATTCGTTTGAAAAGGGTCA‑TGGGTCGCCTCACTGGCC‑TGCCTGCGCGGTCTGCGCAGGCACgg                                                                                                                                  <  1:2954706/150‑1 (MQ=255)
                      tCCCCTGGTAGTAACCCTGCGGCGGTACCAGCGCGGCGTGCGCGGCACCACCAAACAAGGCCAACGCGAGCAGGGCGGGGCTGGAAATTCGTTTGAAAAGGGTCA‑TGGGTCGCCTCACTGGCC‑TGCCTGCGCGGTCTGCGCAGGCACGGc                                                                                                                                 >  2:1089659/1‑150 (MQ=255)
                      tCCCCTGGTAGTAACCCTGCGGCGGTACCAGCGCGGCGTGCGCGGCACCACCAAACAAGGCCAACGCGAGCAGGGCGGGGCTGGAAATTCGTTTGAAAAGGGTCA‑TGGGTCGCCTCACTGGCC‑TGCCTGCGCGGTCTGCGCAGGCACGGc                                                                                                                                 >  1:5076148/1‑150 (MQ=255)
                        cccTGGTAGTAACCCTGCGGCGGTACCAGCGCGGCGTGCGCGGCACCACCAAACAAGGCCAACGCGAGCAGGGCGGGGCTGGAAATTCGTGTGAAAAGGGTCA‑TGGGTCGCCTCACTGGCC‑TGCCTGCGCGGTCTGCGCCGGCACGGCGa                                                                                                                               >  2:2187072/1‑150 (MQ=255)
                         ccgggTAGTAACCCTGCGGCGTTACCCGCGCGGCGTGCGCGGCACCACCAAACAAGGCCAACGCGAGCAGGGGGGGGCTGGAATTTCGTTTGAAAAGGGTCA‑TGGGTCGCCTCACTGGCC‑TGCCTGCGGGGTCTGCGCAGGCACGGCGaa                                                                                                                              <  2:8196393/147‑1 (MQ=255)
                          cTGGTAGTAACCCTGCGGCGGTACCAGCGCGGCGTGCGCGGCACCACCAAACAAGGCCAACGCGAGCAGGGCGGGGCTGGAAATTCGTTTGAAAAGGGTCA‑TGGGTCGCCTCACTGGCC‑TGCCTGCGCGGTCTGCGCAGGCACGGCGAAg                                                                                                                             >  2:2661286/1‑150 (MQ=255)
                          cTGGTAGTAACCCTGCGGCGGTACCAGCGCGGCGTGCGCGGCACCACCAAACAAGGCCAACGCGAGCAGGGCGGGGCTGGAAATTCGTTTGAAAAGGGTCA‑TGGGTCGCCTCACTGGCC‑TGCCTGCGCGGTCTGCGCAGGCACGGCGAAg                                                                                                                             >  2:7305839/1‑150 (MQ=255)
                           tGGTAGTAACCCTGCGGCGGTACCAGCGCGGCGTGCGCGGCACCACCAAACAAGGCCAACGCGAGCAGGGCGGGGCTGGAAATTCGTTTGAAAAGGGTCA‑TGGGTCGCCTCACTGGCC‑TGCCTGCGCGGTCTGCGCAGGCGCGGCGAAgt                                                                                                                            >  1:5352593/1‑150 (MQ=255)
                           tGGTAGTAACCCTGCGGCGGTACCAGCGCGGCGTGCGCGGCACCACCAAACAAGGCCAACGCGAGCAGGGCGGGGCTGGAAATTCGTTTGAAAAGGGTCA‑TGGGTCGCCTCACTGGCC‑TGCCTGCGCGGTCTGCGCAGGCACGGCGAAgt                                                                                                                            >  2:8070002/1‑150 (MQ=255)
                            tttataaaCCCTCCGGCGTTACCAGCGCGGCGTGCGCGCAACAACAAAAAAAGCAAAACGCAAGCAGGGCGGGGATGAAAATTCTTTTTAAAAGGGTCA‑TGGGTCCCCTAACTGGCC‑TCCCTGCGCGCTCTGCCCCGGCACGGCGAAgtt                                                                                                                           <  2:156702/144‑1 (MQ=255)
                              tagtaACCCTGCGGCGGTACCAGCGCGGCGTGCGCGGCACCACCAAACAAGGCCAACGCGAGCAGGGCGGGGCTGGAAATTCGTTTGAAAAGGGTCA‑TGGGTCGCCTCACTGGCC‑TGCCTGCGCGGTCTGCGCAGGCACGGCGACgttgt                                                                                                                         >  2:696875/1‑150 (MQ=255)
                               agtaACCCTGCGGCGGTACCAGCGCGGCGTGCGCGGCACCACCAAACAAGGCCAACGCGAGCAGGGCGGGGCTGGAAATTCGTTTGAAAAGGGTCA‑TGGGTCGCCTCACTGGCC‑TGCCTGCGCGGTCTGCGCAGGCACGGCGAAgttgtt                                                                                                                        >  1:3716189/1‑150 (MQ=255)
                                 taACCCTGCGGGGTTACCCGCGGGGCGTGCGCGCCACCACCAAACACGGCCACGGCGTGCGGGGGGGGGCTGGAATTTGTTTTTAAAGGGGTCT‑TGGGTCGCCTCACTGGCC‑GGCCGGGGGGGTCGGCGCCGGCACGGCGAAGTTGTTGc                                                                                                                      <  2:532243/150‑1 (MQ=255)
                                 taACCCTGCGGCGGTACCCGCGCGGCGTGCGGGGCACCACCACCCACGGCCAACGCGAGCCGGGGGGGGCTGGAATTTCGTTGGAAAAGGGTCA‑TGGGTCGCCCCACGGGCC‑TGCCTGGGGGGTGGGCGCAGGCACGGCGAAGTTGTTGc                                                                                                                      <  2:5106065/150‑1 (MQ=255)
                                  aaCCCTGCGGCGGTACCAGCGCGGCGTGCGCGGCACCACCAAACAAGGCCAACGCGAGCAGGGCGGGGCTGGAAATTCGTTTGAAAAGGGTCA‑TGGGTCGCCTCACTGGCC‑TGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCg                                                                                                                     >  1:4738071/1‑150 (MQ=255)
                                  aaCCCTGCGGCGGTACCAGCGCGGCGTGCGCGGCACCACCAAACAAGGCCAACGCGAGCAGGGCGGGGCTGGAAATTCGTTTGAAAAGGGTCA‑TGGGTCGCCTCACTGGCC‑TGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCg                                                                                                                     >  1:2743013/1‑150 (MQ=255)
                                    cccGGCGGCTGAAGCAGCGCGGGGTGCGCGCCCCCACCAAACAAGGCCAGCGCGAGCTGGGCGGGGCTGTAATTTCGTTTCACATGGGTCA‑TGTGTCGACACACTGGCC‑TTCCTGCGCGGTGTGCGCAGCCACGGCGAAGTTGTTGCGtt                                                                                                                   <  2:2686165/150‑1 (MQ=255)
                                     ccTGCGGCGGGCCCCGCGCGGCGGGCGCGGCACCCCCAACCACGCCCACCGCGTGCCGGGGGGGGCTGTAATTGCGTTTGAAACGGTTCT‑GGGGTCGCCGCACTGGCG‑GGCCGGCGCGGTCGGCGCAGGCACGGCGAAGTTGTTGCGttt                                                                                                                  <  2:7970747/150‑1 (MQ=255)
                                      cTGCGGCGGTACCAGCGCGGCGTGCGCGGCACCACCAAACAAGGCCAACGCGAGCAGGGCGGGGCTGGAAATTCGTTTGAAAAGGGTCA‑TGGGTCGCCTCACTGGCC‑TGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTg                                                                                                                 >  1:2506728/1‑150 (MQ=255)
                                       tGCGGCGGTACCAGCGCGGCGTGCGCGGCACCACCAAACAAGGCCAACGCGAGCAGGGCGGGGCTGGAAATTCGTTTGAAAAGGGTCA‑TGGGTCGCCTCACTGGCC‑TGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGc                                                                                                                >  2:801934/1‑150 (MQ=255)
                                       tGCGGCGGTACCAGCGCGGCGTGCGCGGCACCACCAAACAAGGCCAACGCGAGCAGGGCGGGGCTGGAAATTCGTTTGAAAAGGGTCA‑TGGGTCGCCTCACTGGCC‑TGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGc                                                                                                                >  2:4900627/1‑150 (MQ=255)
                                       tGCGGCGGTACCAGCGCGGCGTGCGCGGCACCACCAAACAAGGCCAACGCGAGCAGGGCGGGGCTGGAAATTCGTTTGAAAAGGGTCA‑TGGGTCGCCTCACTGGCC‑TGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGc                                                                                                                >  2:7082716/1‑150 (MQ=255)
                                              gTACCAGCGCGGCGTGCGCGGCACCACCACACACGGCCACCGCGAGCAGGGCGGGGCTGGAATTTCGATTTAAAAGGGTCA‑TGGGTCGCATCACGGGCC‑GGCCTGCGCGGTCTGCGCTGGCACGGCGAAGTTGTTGCGTTTGCAGAGCtt                                                                                                         <  2:6839811/150‑1 (MQ=255)
                                                 ccAGCGCGGCGTGCGCGGCACCACCAAACAAGGCCAACGCGAGCAGGGCGGGGCTGGAAATTCGTTTGAAAAGGGTCA‑TGGGTCGCCTCACTGGCC‑TGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGc                                                                                                      >  2:8937844/1‑150 (MQ=255)
                                                 ccAGCGCGGCGTGCGCGGCACCACCAAACAAGGCCAACGCGAGCAGGGCGGGGCTGGAAATTCGTTTGAAAAGGGTCA‑TGGGTCGCCTCACTGGCC‑TGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGc                                                                                                      >  1:3779981/1‑150 (MQ=255)
                                                 ccAGCGCGGCGTGCGCGGCACCACCAAACAAGGCCAACGCGAGCAGGGCGGGGCTGGAAATTCGTTTGAAAAGGGTCA‑TGGGTCGCCTCACTGGCC‑TGCCTGCGCGGTCGGCGCAGGCACGGCGAAGTTGGTGCGGTTGCAGAGCTTGGc                                                                                                      >  1:2027674/1‑150 (MQ=255)
                                                  cAGCGCGGCGTGCGCGGTACCACCAAACAAGGCCAACGCGAGCAGGGCGGGGCTGGAAATTCGTTTGAAAAGGGTCA‑TGGGTCGCCTCACTGGCC‑TGCCTGCGCGGTCTGCGCCGGCACGGCGAAGGTGTTGCGGTTGCAGTGCTTGGCt                                                                                                     >  1:7674045/1‑150 (MQ=255)
                                                  cAGCGCGGCGTGCGCGGCACCACCAAACAAGGCCAACGCGAGCAGGGCGGGGCTGGAAATTCGTTTGAAAAGGGTCA‑TGGGTCGCCTCACTGGCC‑TGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCATAGCTTGGCt                                                                                                     >  1:2184702/1‑150 (MQ=255)
                                                      gcggggGGCGCGGCACCCCCAACCCCGGCCACCGGGCGCGGGGGGGGGGTGGAATTTGGTTTGAACCGGGTCC‑TGGGGCGCCTCCGGGGCC‑GGGGGGGGGGGTGGGCGCGGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGa                                                                                                 <  2:2563756/150‑1 (MQ=255)
                                                          cgTGCGCGGCACCACCAAACAAGGCCAACGCGAGCAGGGCGGGGCTGGAAATTCGTTTGAAAAGGGTCA‑TGGGTCGCCTCACTGGCC‑TGCCTGCCGGGGCTGCGCAGGCACGGCCTAGATGGTGGGTTTGCAGAGCATGGCATCGAccgg                                                                                             >  1:868187/1‑148 (MQ=255)
                                                           gTGCGCGGCACCACCAACCAAGGCCACCGCGAGCTGGGCGGGGCTGGAAATTCTTTTGAAAAGGGTCA‑TGGGTCGCCTCACTGGCC‑GGCCTGCGCGGTCTGCGCTGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTc                                                                                            <  2:9066148/150‑1 (MQ=255)
                                                            tGCGCGCCACCACAAAACACGGCCACCGCGCGCGGGGGGGGGCTTTAATTTCGTTTCAAATGGGTCA‑GGGGCCGCCTCAGGGGCC‑TGCGGGCGGGGGCGGCGCCGCCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCt                                                                                           <  1:6261579/150‑1 (MQ=255)
                                                             gcgcGGCCCCACCAAACCCGGCCACCGCGCGCCGGGGGGGGCTGGAATTTGTTTTCAAACGGGTCC‑TGGGTCGCCTCCGGGGCC‑TGCCTGCGGGGCCGGCGCCGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTg                                                                                          <  2:3164546/150‑1 (MQ=255)
                                                              cgcgGCACCACAAAACACGGCCACCGCGAGCTGGGGGGGGCTGTAATTTGTTTTTAAATGGGTCT‑TGGGGCGCCTCCCGGGCC‑TGCCGGCGGGGCGTGCGCCGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTgg                                                                                         <  1:9015620/150‑1 (MQ=255)
                                                                  gCACCACCAAACAAGGCCAACGCGAGCAGGGCGGGGCTGGAAATTCGTTTGAAAAGGGTCA‑TGGGTCGCCTCACTGGCC‑TGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGTGCTTGGCTTCGACCTTCTGGGTGc                                                                                     <  2:8168355/150‑1 (MQ=255)
                                                                       accaAACAAGGCCAACGCGAGCAGGGCGGGGCTGGAAATTCGTTTGAAAAGGGTCA‑TGGGTCGCCTCACTGGCC‑TGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCtt                                                                                >  1:6229791/1‑150 (MQ=255)
                                                                         caAACAAGGCCAACGCGAGCAGGGCGGGGCTGTAAATTCGTTTGAAAAGGGTCA‑TGGGTCGCCTCACTGGCC‑TGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTTCAGAGCTTGGCTTAGACCTTCTGGGTGCCGCTTTc                                                                              >  2:3907130/1‑150 (MQ=255)
                                                                             cacggCCACCGCGAGCGGGGCGGGGCTGCAAATTCTTTTTAACCGGGTCT‑TGGGCCGCCTCACGGGCC‑GGCCGGCGCGGTCGGCGCCGCCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGc                                                                          <  2:433507/147‑1 (MQ=255)
                                                                             cacgcccACCGCGCGCCGGGGGGGGCTGCAATTTGTTTTGAACCGGGTCT‑TGGGGCGCCTCACGGGCC‑GGCCGGGGGGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGc                                                                          <  2:4578366/145‑1 (MQ=255)
                                                                               cggCCAAGGCGAGCAGGGCGGGGCTGGAAATTGTTTTGAAACGGGTCT‑TGGGGCGCCTCCCTGGCC‑TGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGccc                                                                        <  2:5570456/149‑1 (MQ=255)
                                                                               cgcccAACGCGAGCTGGGCGGGGCTGGAAATTCGTTTTAAATGGGTCT‑TGGGTCGCCCCACTGGCC‑GGCCTGCGGGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGccc                                                                        <  2:2397509/147‑1 (MQ=255)
                                                                                   caccgcgAGCGGGGGGGGGGTTTAATTTCGTTTCAAACGGGTAT‑GGGGGCGCCTCACTGGCG‑GGCCTGCGGGGTCGGCGCAGCCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGt                                                                    <  1:4757742/147‑1 (MQ=255)
                                                                                   cAACGCGAGCAGGGCGGGGCTGGAAATTCGTTTGAAAAGGGTCA‑TGGGTCGCCTCACTGGCC‑TGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGt                                                                    >  1:4279847/1‑150 (MQ=255)
                                                                                   cAACGCGAGCAGGGCGGGGCTGGAAATTCGTTTGAAAAGGGTCA‑TGGGTCGCCTCACTGGCC‑TGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGt                                                                    >  2:6098893/1‑150 (MQ=255)
                                                                                   cAACGCGAGCAGGGCGGGGCTGGAAATTCGTTTGAAAAGGGTCA‑TGGGTCGCCTCACTGGCC‑TGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCGGt                                                                    >  1:1415793/1‑150 (MQ=255)
                                                                                   cAACGCGAGCAGGGCGGGGCTGGAAATTCGTTTGAAAAGGGTCA‑TGGGTCGCCTCACTGGCC‑TGCCTGCGCGGTCTGCGCAGGCACCGCGAAGTTGTTTCGTTTGCAGAGCTTGGCTTAGACCATATGGGTGCCGCATTACGGCCCCAGt                                                                    >  1:8518892/1‑150 (MQ=255)
                                                                                    accgcgcgCCGGGCGGGGGTGGAATTGGGTTGTAAACGGGTCT‑GGGGTCGCCTCCCTGGCC‑TGCGTGCGGGGTCTGCGCCGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGccccagaa                                                                   <  2:2419470/148‑5 (MQ=255)
                                                                                    aaCGCGCGCTGGGGGGGGCTGTAATTTGGTTTTAAATGGGCCT‑GGGGCCGCCACACGGGCC‑GGCCGGCGCGGTCTGCGCAGCCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGTa                                                                   <  1:2162707/150‑1 (MQ=255)
                                                                                     cggcgcgcCGGGGGGGGGTGTAAATTCGTTTGAAACGGGTCT‑GGGGTCGCCTCACTGGCC‑GGCCGGGGGGGTGTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGTAc                                                                  <  2:7814017/148‑1 (MQ=255)
                                                                                     aCGCGAGCAGGGCGGGGCTGGAAATTCGTTTGAAAAGGGTCA‑TGGGTCGCCTCACTGGCC‑TGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGTAc                                                                  >  2:3525411/1‑150 (MQ=255)
                                                                                      cgcgcgCTGGGCGGGGCGGGAAATTCGTTTCAAATGGGGCG‑GGGGGCGCCTCCGGGGCG‑GGCGGGCGCGGGCGGCGCCGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGTAcc                                                                 <  1:5740407/150‑1 (MQ=255)
                                                                                      cgcgAGCTGGGCGGGGCTGGAAATTCGTTTTAAATGGGTCT‑GGGGTCGCCTCACTGGCC‑TGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGTAcc                                                                 <  1:8342813/150‑1 (MQ=255)
                                                                                       gcgAGCAGGGCGGGGCTGGAAATTCGTTTGAAAAGGGTCA‑TGGGTCGCCTCACTGGCC‑TGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGTACCt                                                                >  2:5708902/1‑150 (MQ=255)
                                                                                        cgAGCTGGGCGGGGCTGGAAATTCGTTTTAAATGGGTCA‑TGGGTCGCCTCACTGGCC‑TGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGTACCTc                                                               <  2:5340300/150‑1 (MQ=255)
                                                                                        cgAGCTGGGCGGGGCTGGAAATTCGTTTGAAATGGGTCA‑TGGGTCGCCTCACTGGCC‑TGCCGGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGTACCTc                                                               <  2:8362191/150‑1 (MQ=255)
                                                                                         gAGCAGGGCGGGGCTGGAAATTCGTTTGAAAAGGGTCA‑TGGGTCGCCTCACTGGCC‑TGCCAGCGCGGTCTGCGCAGTCACGGCGAAGTTGTTTCGTTTTCAGAGCTTGGCTTCGACCATCTGGGTGCCGCTTTCCGGCCCCGGTACCTCg                                                              >  2:44709/1‑150 (MQ=255)
                                                                                             aGGGCGGGGCTGGAAATTCGTTTGAAAAGGGTCA‑TGGGTCGCCTCACTGGCC‑TGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGTACCTCGAAcg                                                          >  2:6345475/1‑150 (MQ=255)
                                                                                              ggggggggCTGTATTTTGTTTTTAAATGGGTCT‑TGGGCCGCCTCCGGGGCG‑TGCGTGGGGGGTCGGCGCCGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGTACCTCGAAcgc                                                         <  1:6340424/150‑1 (MQ=255)
                                                                                              ggggggggCTGTAATTTGTTTTTAAATGGGGCC‑TGGGTCGCCTCCCTGGCC‑GGCCGGCGCGGTCGGCGCCGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGTACCTCGAAcgc                                                         <  1:2834711/150‑1 (MQ=255)
                                                                                              gggCGGGGCTGTAAATTCGTTTGAAATGGGACT‑TGGGTCGCCCCACTGCCC‑GGCCGGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGTACCTCGAAcgc                                                         <  2:300396/150‑1 (MQ=255)
                                                                                               ggCGGGGCTGGAAATTCGTTTGAAAAGGGTCA‑TGGGTCGCCTCACTGGCC‑TGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGTACCTCGAAcgcg                                                        >  2:7456233/1‑150 (MQ=255)
                                                                                                      cTGGAAATTCGTTTGAAAAGGGTCA‑TGGGTCGCCTCACTGGCC‑TGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCATCTGGGTGCCGCTTTCCGGCCCCTGTACCTCGAACGCGAGCAGgc                                                 >  1:7020558/1‑150 (MQ=255)
                                                                                                        tgAAATTGGTTTTAAAAGGGACT‑TGGGTCGCCTCACTGGCC‑TGCCTGCGCGTCCTGCGCTGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGTACCTCGAACGCGAGCAGgcgc                                               <  2:2974559/149‑1 (MQ=255)
                                                                                                         gAATTTCGTTTTAAATGGGTCT‑TGGGTCGCCTCACTGGCC‑GGCCTGCGCGGGCGGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGTACCTCGAACGCGAGCAGGCgct                                              <  2:2056436/150‑1 (MQ=255)
                                                                                                              tCGTTTGAAAAGGGTCA‑TGGGTCGCCTCACTGGCC‑TGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGTACCTCGAACGCGAGCAGGCgctggctg                                         >  1:2915820/1‑150 (MQ=255)
                                                                                                                 tttGAAAAGGGTCA‑TGGGTCGCCTCACTGGCC‑TGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGTACCTCGAACGCGAGCAGGCGCTGGCTGGcc                                      >  2:5488389/1‑150 (MQ=255)
                                                                                                                    gAAAAGGGTCA‑TGGGTCGCCTCACTGGCC‑TGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGAACTTCTGGGTGCCGCTTTCCGGCCCCTGTACCTCGAACGCGAGCCGGCGCGGGCTGGCCCAg                                   >  1:532085/1‑150 (MQ=255)
                                                                                                                     aaaaGGGTCA‑TGGGTCGCCTCACTGGCC‑TGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGTACCTCGAACGCGAGCAGGCGCTGGCTGGCCCAGt                                  >  2:8693770/1‑150 (MQ=255)
                                                                                                                      aaaTGGTCATTGGGTCGCCTCAGTGGCC‑GGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGTACCTCGAACGCGAGCAGGCGCTGGCTGGCCCAGt                                  <  2:3417200/150‑1 (MQ=255)
                                                                                                                      aaaGGGTCA‑TGGGTCGCCTCACTGGCC‑TGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGGGTTTTCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGTACCTCGAACGCGAGCAGGCGCTGGCTGGCCCAGTc                                 >  1:3007912/1‑150 (MQ=255)
                                                                                                                      aaaGGGTCA‑TGGGTCGCCTCACTGGCC‑TGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTAGACATTCTGGGTGCCGCTTTCCGGCCCCTGTACCTCGAACGCGAGCAGGCGCTGGCTGGCCCAGTc                                 >  2:923310/1‑150 (MQ=255)
                                                                                                                        tgggTCC‑GGGGTCGCCTCCGGGGCC‑GGCCGGGGGGGTCGGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGTACCTCGAACGCGAGCAGGCGCTGGCTGGCCCAGTCCt                               <  1:3993843/149‑1 (MQ=255)
                                                                                                                         gggTCA‑TGGGTCGCCTCCCGGGCC‑GGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGTACCTCGAACGCGAGCAGGCGCTGGCTGGCCCAGTCCTc                              <  2:6757159/150‑1 (MQ=255)
                                                                                                                            tCA‑TGGGTCGCCTCACTGGCC‑TGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTTTTGCGTTTGCAGAGCTTTGCTTAGACCGTCTGGGTGCCGCTTTCCGGCCCCAGTACCACGAACGCGAGCAGGCGCAGGCTGGCCCAGTCCGCGTc                           >  1:6999309/1‑150 (MQ=255)
                                                                                                                              a‑TGGGTCGCCTCACTGGCC‑TGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGTACCTCGAACGCGAGCAGGCGCTGGCTGGCCCAGTCCTCGTCtt                         >  2:4239571/1‑150 (MQ=255)
                                                                                                                                            ggggCC‑TGCGGGCGGGGCCGGCGCCGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGTACCTCGAACGCGAGCAGGCGCTGGCTGGCCCAGTCCTCGTCTTCGCGCATCTGGaa            <  1:2661286/148‑1 (MQ=255)
                                                                                                                                             tGGCC‑TGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGTACCTCGAACGCGAGCAGGCGCTGGCTGGCCCAGTCCTCGTCTTCGCGCATCTGGAAg           >  2:6727026/1‑150 (MQ=255)
                                                                                                                                              ggCC‑TGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGTACCTCGAACGCGAGCAGGCGCTGGCTGGCCCAGTCCTCGTCTTCGCGCATCTGGAAGa          >  2:8621923/1‑150 (MQ=255)
                                                                                                                                                 cgtgcctgcGCAGACCGCGCAGGCA‑GGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGTACCTCGAACGCGAGCAGGCGCTGGCTGGCCCAGTCCTCGTCTTCGCGCATCTGGAagaaga       >  1:4898833/3‑150 (MQ=255)
                                                                                                                                                 c‑tgcctgcGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGTACCTCGAACGCGAGCAGGCGCTGGCTGGCCCAGTCCTCGTCTTCGCGCATCTGGAAGACGa       >  2:5221085/1‑150 (MQ=255)
                                                                                                                                                 c‑tgcctgcGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGTACCTCGAACGCGAGCAGGCGCTGGCTGGCCCAGTCCTCGTCTTCGCGCATCTGGAAGACGa       >  1:8103158/1‑150 (MQ=255)
                                                                                                                                                       tgcGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGTACCTCGAACGCGAGCAGGCGCTGGCTGGCCCAGTCCTCGTCTTCGCGCATCTGGAAGACGAAGCgg  >  1:1766561/1‑150 (MQ=255)
                                                                                                                                                       tgcGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGTACCTCGAACGCGAGCAGGCGCTGGCTGGCCCAGTCCTCGTCTTCGCGCATCTGGAAGACGAAGCgg  >  1:7864062/1‑150 (MQ=255)
                                                                                                                                                       |                                                                                                                                                     
TCACCGGTCTTGAGCTTTTCGATCCCCTGGTAGTAACCCTGCGGCGGTACCAGCGCGGCGTGCGCGGCACCACCAAACAAGGCCAACGCGAGCAGGGCGGGGCTGGAAATTCGTTTGAAAAGGGTCA‑TGGGTCGCCTCACTGGCC‑TGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGTACCTCGAACGCGAGCAGGCGCTGGCTGGCCCAGTCCTCGTCTTCGCGCATCTGGAAGACGAAGCGG  >  NC_002947/1464049‑1464347

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑