BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,488,064	T→A	8.0%	K89M (AAG→ATG) ‡	degS ←	serine endoprotease DegS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,488,064	0	T	A	8.0%	1125.4 / 59.0	355	K89M (AAG→ATG) ‡	degS	serine endoprotease DegS
Reads supporting (aligned to +/- strand): ref base T (166/160); new base A (12/16); total (179/176)
Fisher's exact test for biased strand distribution p-value = 4.38e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 362 total aligned reads displayed.

GTTGTTGGTCAGCAGGTAGCCCTCTGGGCTCATGATTACCGCCGAACCCAGGCTTGATTCCCAGCGGCGCTGTTTGGGCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCGGGTAGTCTGTGGCGCTTGCTGCAGGTTGACGTCCTGGCTGGGCAGCCCGACCCATTGCGGAAAACGCTGG  >  NC_002947/1487916‑1488213

gttgttGGTCAGCAGGTAGCCCTCTGGGCTCATGATTACCGCCGAACCCAGGCTTGATTCCCAGCGGCGCTGTTTGGGCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCtt                                                                                                                                                      <  2:2811527/150‑1 (MQ=255)

gttgttGGTCAGCAGGTAGCCCTCTGGGCTCATGATTACCCCCGAACCCAGGCTTGATACCCAGAGGCGCAGTATGGGCAGGTTATCTCCGAAGAAGAGGCGGAACTGCGGGACTTCGAACAGCGGGTGGGCGCTTTTGATCACCACCtt                                                                                                                                                      >  2:8016171/1‑150 (MQ=255)

   gttgGTCAGCAGGTAGCCCTCTGGGCTCATGATTACCGCCGAACCCAGGCTTGATTCCCAGCGGCGCTGTTTGGGCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTtggt                                                                                                                                                   >  1:5663773/1‑150 (MQ=255)

    ttgGTCAGCAGGTAGCCCTCTGGGCTCATGATTACCGCCGAACCCAGGCTTGATTCCCAGCGGCGCTGTTTGGGCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTtggtg                                                                                                                                                  <  1:7407447/150‑1 (MQ=255)

      ggTCAGCAGGTAGCCCTCTGGGCTCATGATTACCGCCGAACCCAGGCTTCATTCCCAGCGGCGCTGTTTGGGCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTggtggt                                                                                                                                                >  1:1030298/1‑150 (MQ=255)

      ggTCAGCAGGTAGCCCTCTGGGCTCATGATTACCGCCGAACACAGGCTTGATTCCCAGCGGCGCTGTTTGGGCAGGTTATCTCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTAAACACCTTggtggt                                                                                                                                                >  2:6156542/1‑150 (MQ=255)

        tCAGCAGGTAGCCCTCTGGGCTCATGATTACCGCCGAACCCAGGCTTGATTCCCAGCGGCGCTGTTTGGGCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGgtgt                                                                                                                                              >  1:5294420/1‑150 (MQ=255)

        tCAGCAGGTAGCCCTCTGGGCTCATGATTACCGCCGAACCCAGGCTTGATTCCCAGCGGCGCTGTTTGGGCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGgtgt                                                                                                                                              >  1:720824/1‑150 (MQ=255)

         cagcagGTAGCCCTCTGGGCTCATGATTACCGCCGAACCCAGGCTTGATTCCCAGCGGCGCTGTTTGGGCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCAAGgtggtgaa                                                                                                                                             <  1:6572932/150‑3 (MQ=255)

          agcagGTAGCCCTCTGGGCTCATGATTACCGCCGAACCCAGGCTTGATTCCCAGCGGCGCTGTTTGGGCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTAc                                                                                                                                            <  1:4848104/150‑1 (MQ=255)

          agcagGTAGCCCTCTGGGCTCATGATTACCGCCGAACCCAGGCTTGATTCCCAGCGGCGCTGTTTGGGCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTAc                                                                                                                                            >  2:7294613/1‑150 (MQ=255)

            cagGTAGCCCTCTGGGCTCATGATTACCGCCGAACCCAGGCTTGATTCCCAGCGGCGCTGTTTGGGCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACAg                                                                                                                                          >  2:8626605/1‑150 (MQ=255)

            cagGTAGCCCTCTGGGCTCATGATTACCGCCGAACCCAGGCTTGATTCCCAGCGGCGCTGTTTGGGCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCAAGGTGGTGAACaa                                                                                                                                          <  1:3856401/150‑2 (MQ=255)

              ggTAGCCCTGTGGGCTCATTATTACCGCCGACCCCAGGCTTGATTCCCAGCGGCGCTGTTTGGGCATGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACaaaa                                                                                                                                        <  1:4057954/150‑4 (MQ=255)

              ggTAGCCCTCTGGGCTCATGATTACCGCCGAACCCAGGCTTGATTCCCAGCGGCGCTGTTTGGGCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACAGGt                                                                                                                                        <  2:3052947/150‑1 (MQ=255)

              ggTAGCCCTCTGGGCTCATGATTACCGCCGAACCCAGGCTTGATTCCCAGCGGCGCTGTTTGGGCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCAAGGTGGTGAACaaaa                                                                                                                                        <  2:8832443/150‑4 (MQ=255)

              ggTAGCCCTCTGGGCTCATGATTACCGCCGAACCCAGGCTTGATTCCCAGCGGCGCTGTTTGGGCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTACACCACCAAGGTGGTGAACaaaa                                                                                                                                        <  1:472534/150‑4 (MQ=255)

               gTAGCCCTCTGGGCTCATGATTACCGCCGAACCCAGGCTTGATTCCCAGCGGCGCTGTTTGGGCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACAGGtt                                                                                                                                       <  1:6363005/150‑1 (MQ=255)

               gTAGCCCTCTGGGCTCATGATTACCGCCGAACCCAGGCTTGATTCCCAGCGGCGCTGTTTGGGCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACAGGtt                                                                                                                                       >  2:4753541/1‑150 (MQ=255)

                 aGCCCTCTGGGCTCATGATTACCGCCGAACCCAGGCTTGATTCCCAGCGGCGCTGTTTGGGCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACAGGTTgg                                                                                                                                     >  1:781571/1‑150 (MQ=255)

                  gCCCTCTGGGCTCATGATTACCGCCGAACCCAGGCTTGATTCCCAGCGGCGCTGTTTGGGCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACAGGTTGGc                                                                                                                                    <  1:2599547/150‑1 (MQ=255)

                    ccTCTGGGCTCATGATTACCGCCGAACCCAGGCTTGATTCCCAGCGGCGCTGTTTGGGCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACAGGTTGGCCa                                                                                                                                  <  1:4976069/150‑1 (MQ=255)

                        tGGGCTCATGATTACCGCCGAACCCAGGCTTGATTCCCAGCGGCGCTGTTTGGGCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACAGGTTGGCCAccgc                                                                                                                              >  1:2096176/1‑150 (MQ=255)

                         gggCTCATGATTACCGCCGAACCCAGGCTTGATTCCCAGCGGCGCTGTTTGGGCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACAGGTTGGCCAccgcc                                                                                                                             <  2:7982301/150‑1 (MQ=255)

                          ggCTCATGATTACCGCCGAACCCAGGCTTGATTCCCAGCGGCGCTGTTTGGGCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACAGGTTGGCCAccgccg                                                                                                                            <  2:7115098/150‑1 (MQ=255)

                          ggCTCATGATTACCGCCGAACCCAGGCTTGATTCCCAGCGGCGCTGTTTGGGCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACAGGTTGGCCAccgccg                                                                                                                            >  2:2424330/1‑150 (MQ=255)

                             tCATGATTACCGCCGAACCCAGGCTTGATTCCCAGCGGCGCTGTTTGGGCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACAGGTTGGCCACCGCCgggg                                                                                                                         >  1:2989387/1‑150 (MQ=255)

                             tCATGATTACCGCCGAACCCAGGCTTGATTCCCAGCGGCGCTGTTTGGGCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACAGGTTGGCCACCGCCgggg                                                                                                                         >  1:6782624/1‑150 (MQ=255)

                             tCATGATTACCGCCGAACCCAGGCTTGATTCCCAGCGGCGCTGTTTGGGCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACAGGTTGGCCACCGCCgggg                                                                                                                         >  1:6057989/1‑150 (MQ=255)

                              cATGATTACCGCCGAACCCAGGCTTGATTCCCAGCGGCGCTGTTTGGGCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACAGGTTGGCCACCGCCGGggc                                                                                                                        <  1:1846762/150‑1 (MQ=255)

                              cATGATTACCGCCGAACCCAGGCTTGATTCCCAGCGGCGCTGTTTGGGCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACAGGTTGGCCACCGCCGGggc                                                                                                                        <  1:2742913/150‑1 (MQ=255)

                               aTGATTACCGCCGAACCCAGGCTTGATTCCCAGCGGCGCTGTTTGGGCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACAGGTTGGCCACCGCCGGggcg                                                                                                                       >  1:3473448/1‑150 (MQ=255)

                                 gATTACCGCCGAACCCAGGCTTGATTCCCAGCGGCGCTGTTTGGGCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACAGGTTGGCCACCGCCGGggcggc                                                                                                                     >  1:5398743/1‑150 (MQ=255)

                                     aCCGCCGAACCCAGGCTTGATTCCCAGCGGCGCTGTTTGGGCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACAGGTTGGCCGCCCCGGCGGTGGCCaac                                                                                                                 <  2:3459962/150‑3 (MQ=255)

                                     aCCGCCGAACCCAGGCTTGATTCCCAGCGGCGCTGTTTGGGCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACAGGTTGGCCACCGCCGGGGCGGCCAgg                                                                                                                 <  2:6693146/150‑1 (MQ=255)

                                         ccgAACCCAGGCTTGATTCCCAGCGGCGCTGTTTGGGCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCa                                                                                                             >  1:8675573/1‑150 (MQ=255)

                                            aaCCCAGGCTTGATTCCCAGCGGCGCTGTTTGGGCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCAcgg                                                                                                          >  2:1771607/1‑150 (MQ=255)

                                               ccAGGCTTGATTCCCAGCGGCGCTGTTTGGGCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCAcggcgt                                                                                                       >  2:1291282/1‑150 (MQ=255)

                                                 aGGCTTGATTCCCAGCGGCGCTGTTTGGGCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCAcggcgtcg                                                                                                     >  1:445032/1‑150 (MQ=255)

                                                  ggCTTGATTCCCAGCGGCGCTGTTTGGGCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCAcggcgtcgg                                                                                                    <  1:971903/150‑1 (MQ=255)

                                                     ttGATTCCCAGCGGCGCTGTTTGGGCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTggtggt                                                                                                                                                >  1:6884410/1‑103 (MQ=255)

                                                     ttGATTCCCAGCGGCGCTGTTTGGGCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTggtggt                                                                                                                                                <  2:6884410/103‑1 (MQ=255)

                                                      tGATTCCCAGCGGCGCTGTTTGGGCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTa                                                                                                <  2:5988470/150‑1 (MQ=255)

                                                             cAGCGGCGCTGTTTGGGCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACAcc                                                                                         >  2:2189912/1‑150 (MQ=255)

                                                             cAGCGGCGCTGTTTGGGCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACAcc                                                                                         <  2:4244934/150‑1 (MQ=255)

                                                                cggcgCTGTTTGGGCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCggg                                                                                      <  1:3306520/150‑1 (MQ=255)

                                                                    gcTGTTTGGGCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACAGGGCCtt                                                                                  <  1:3546143/150‑1 (MQ=255)

                                                                     cTGTTTGGGCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTg                                                                                 <  1:8455550/150‑1 (MQ=255)

                                                                       gTTTGGGCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCa                                                                               >  2:8426319/1‑150 (MQ=255)

                                                                            ggCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATc                                                                          <  2:4536156/150‑1 (MQ=255)

                                                                               aGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCgg                                                                       <  1:2189912/150‑1 (MQ=255)

                                                                               aGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCgg                                                                       <  2:7350210/150‑1 (MQ=255)

                                                                                      cGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCGGGTAGTCt                                                                <  1:8250862/150‑1 (MQ=255)

                                                                                        ccGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCgg                                                                       <  2:210124/141‑1 (MQ=255)

                                                                                         cGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCGGGTAGTCtgtg                                                             <  2:1543208/150‑1 (MQ=255)

                                                                                         cGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCGGGTAGTCtgtg                                                             <  1:4091497/150‑1 (MQ=255)

                                                                                         cGAAGAAGCGGCGGAACTGCGGGCCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACAGGTGGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCGGGTAGTCtgtg                                                             <  1:181103/150‑1 (MQ=255)

                                                                                              aagCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCGGGTAGTCTGTGGCGCt                                                        >  2:1972061/1‑150 (MQ=255)

                                                                                                gcggcgGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCGGGTAGTCTGTGGCGCTtg                                                      >  2:2583638/1‑150 (MQ=255)

                                                                                                gcggcgGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCGTTTGTTCACCACCTTGGTGGTGGACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCGGGTAGTCTGTGGCGCtgg                                                      >  2:9066029/1‑148 (MQ=255)

                                                                                                   gcggAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCGGGTAGTCTGTGGCGCTtgctg                                                   <  2:4790471/150‑1 (MQ=255)

                                                                                                      gAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCGGGTAGTCTGTGGCGCTTGCTGCAg                                                <  2:5647381/150‑1 (MQ=255)

                                                                                                         cTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCGGGTAGTCTGTGGCGCTTGCTGCAGGtt                                             <  1:8953263/150‑1 (MQ=255)

                                                                                                          tGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCGGGTAGTCTGTGGCGCTTGCTGCAGGTTg                                            <  1:8334750/150‑1 (MQ=255)

                                                                                                            cGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCGGGTAGTCTGTGGCGCTTGCTGCAGGTTGAc                                          <  2:4828649/150‑1 (MQ=255)

                                                                                                            cGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCGGGTAGTCTGCGGCGCTTGCTGCAGGTTGAc                                          <  2:781571/150‑1 (MQ=255)

                                                                                                                 cTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCGGGTAGTCTGTGGCGCTTGCTGCAGGTTGACGTCct                                     <  2:6735632/150‑1 (MQ=255)

                                                                                                                 cTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCGGGTAGTCTGTGGCGCTTGCTGCAGGTTGACGTCct                                     <  1:7551234/150‑1 (MQ=255)

                                                                                                                  ttCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCGGGTAGTCTGTGGCGCTTGCTGCAGGTTGACGTCctg                                    >  2:7638791/1‑150 (MQ=255)

                                                                                                                  ttCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCGGGTAGTCTGTGGCGCTTGCTGCAGGTTGACGTCctg                                    >  1:8841855/1‑150 (MQ=255)

                                                                                                                  ttCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCGGGTAGTCTGTGGCGCTTGCTGCAGGTTGACGTCctg                                    >  2:386536/1‑150 (MQ=255)

                                                                                                                  ttCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCGGGTAGTCTGTGGCGCTTGCTGCAGGTTGACGTCctg                                    >  1:7989565/1‑150 (MQ=255)

                                                                                                                     gAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCGGGTAGTCTGTGGCGCTTGCTGCAGGTTGACGTCctggct                                 >  1:1355567/1‑150 (MQ=255)

                                                                                                                         aGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCGGGTAGTCTGTGGCGCTTGCTGCAGGTTGACGTCCTGGCTGGGc                             >  1:6217207/1‑150 (MQ=255)

                                                                                                                            gggTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCGGGTAGTCTGTGGCGCTTGCTGCAGGTTGACGTCCTGGCTGGGCAGc                          <  2:2620552/150‑1 (MQ=255)

                                                                                                                              gTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCGGGTAGTCTGTGGCGCTTGCTGCAGGTTGACGTCCTGGCTGGGCAGccc                        >  2:5843778/1‑150 (MQ=255)

                                                                                                                                   cgcTTTTGTTCACCACCTTGGTGGTGTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCGGGTAGTCTGTGGCGCTTGCTGCAGGTTGACGTCCTGGCTGGGCAGCCCGAccc                   >  2:4380996/1‑150 (MQ=255)

                                                                                                                                      ttttGTTCACCACCTTGGTGGTGTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCGGGTAGTCTGTGGCGCTTGCTGCAGGTTGACGTCCTGGCTGGGCAGCCCGACCCAtt                >  1:524157/1‑150 (MQ=255)

                                                                                                                                      ttttGTTCACCACCTTGGTGGTGTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCGGGTAGTCTGTGGCGCTTGCTGCAGGTTGACGTCCTGGCTGGGCAGCCCGACCCAtt                >  1:128945/1‑150 (MQ=255)

                                                                                                                                      ttttGTTCACCACCTTGGTGGTGTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCGGGTAGTCTGTGGCGCTTGCTGCAGGTTGACGTCCTGGCTGGGCAGCCCGACCCAtt                >  1:1973022/1‑150 (MQ=255)

                                                                                                                                      ttttGTTCACCACCTTGGTGGTGTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCGGGTAGTCTGTGGCGCTTGCTGCAGGTTGACGTCCTGGCTGGGCAGCCCGACCCAtt                >  2:6386696/1‑150 (MQ=255)

                                                                                                                                         tGTACACCACCAAGGTGGTGTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCGGGTAGTCTGTGGCGCTTGCTGCAg                                                >  2:5193638/1‑115 (MQ=255)

                                                                                                                                         tGTACACCACCAAGGTGGTGTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCGGGTAGTCTGTGGCGCTTGCTGCAGGTTGACGTCCTGGCTGGGCAGCCCGACCCATTGCg             >  2:3252775/1‑150 (MQ=255)

                                                                                                                                         tGTACACCACCAAGGTGGTGTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCGGGTAGTCTGTGGCGCTTGCTGCAGGTTGACGTCCTGGCTGGGCAGCCCGACCCATTGCg             >  1:4249847/1‑150 (MQ=255)

                                                                                                                                         tGTACACCACCAAGGTGGTGAACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCGGGTAGt                                                                  >  1:5754002/1‑97 (MQ=255)

                                                                                                                                         tGTACACCACCAAGGTGGTGAACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCGGGTAGTCTGTGGCGCTTGCTGCAGGTTGACGTCCTGGCTGGGCAGCCCGACCCATTGCg             >  1:5836747/1‑150 (MQ=255)

                                                                                                                                           ttCACCACCTTGGTGGTGTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCGGGTAGTCTGTGGCGCTTGCTGCAGGTTGACGTCCTGGCTGGGTAGCCCGACCCATTGCGGa           >  2:8787905/1‑150 (MQ=255)

                                                                                                                                              accaccTTGGTGGTGTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCGGGTAGTCTGTGGCGCTTGCTGCAGGTTGACGTCCTGGCTGGGCCGCCCGACCCATTGCGGaaaa        >  2:2956690/1‑150 (MQ=255)

                                                                                                                                              accaccTTGGTGGTGTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCGGGTAGTCTGTGGCGCTTGCTGCAGGTTGACGTCCTGGCTGGGCAGCCCGACCCATTGCGGaaaa        <  1:1643244/150‑1 (MQ=255)

                                                                                                                                                caccTTGGTGGTGTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCGGGTAGTCTGTGGCGCTTGCTGCAGGTTGACGTCCTGGCTGGGCAGCCCGACCCATTGCGGAAAACg      >  2:541368/1‑150 (MQ=255)

                                                                                                                                                  ccTTGGTGGTGTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCGGGTAGTCTGTGGCGCTTGCTGCAGGTTGACGTCCTGGCTGGGCAGCCCGACCCATTGCGGAAAACGCt    <  2:3728181/150‑1 (MQ=255)

                                                                                                                                                  ccTTGGTGGTGTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCGGGTAGTCTGTGGCGCTTGCTGCAGGTTGACGTCCTGGCTGGGCAGCCCGACCCATTGCGGAAAACGCt    <  2:4025214/150‑1 (MQ=255)

                                                                                                                                                  ccTTGGTGGTGTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCGGGTAGTCTGTGGCGCTTGCTGCAGGTTGACGTCCTGGCTGGGCAGCCCGACCCATTGCGGAAAACGCt    <  1:3698998/150‑1 (MQ=255)

                                                                                                                                                  ccTTGGTGGTGTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCGGGTAGTCTGTGGCGCTTGCTGCAGGTTGACGTCCTGGCTGGGCAGCCCGACCCATTGCGGAAAACGCt    <  1:2812509/150‑1 (MQ=255)

                                                                                                                                                  ccTTGGTGGTGTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCGGGTAGTCTGTGGCGCTTGCTGCAGGTTGACGTCCTGGCTGGGCAGCCCGACCCATTGCGGAAAACGCt    <  2:8330132/150‑1 (MQ=255)

                                                                                                                                                  ccTTGGTGGTGTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCGGGTAGTCTGTGGCGCTTGCTGCAGGTTGACGTCCTGGCTGGGCAGCCCGACCCATTGCGGAAAACGCt    <  2:5723161/150‑1 (MQ=255)

                                                                                                                                                  ccTTGGTGGTGTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCGGGTAGTCTGTGGCGCTTGCTGCAGGTTGACGTCCTGGCTGGGCAGCCCGACCCATTGCGGAAAACGCt    <  2:1134160/150‑1 (MQ=255)

                                                                                                                                                   cTTGGTGGTGTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCTCGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCGGGTAGTCTGTGGCGCTTGCTGCAGGTTGACGTCCTGGCTGGGCAGCCCGACCCATTGCGGAAAACGCTg   <  1:8016171/150‑1 (MQ=255)

                                                                                                                                                   cTTGGTGGTGTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCGGGTAGTCTGTGGCGCTTGCTGCAGGTTGACGTCCTGGCTGGGCAGCCCGACCCATTGCGGAAAACGCTg   <  1:699913/150‑1 (MQ=255)

                                                                                                                                                   cTTGGTGGTGTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCGGGTAGTCTGTGGCGCTTGCTGCAGGTTGACGTCCTGGCTGGGCAGCCCGACCCATTGCGGAAAACGCTg   <  2:1722874/150‑1 (MQ=255)

GTTGTTGGTCAGCAGGTAGCCCTCTGGGCTCATGATTACCGCCGAACCCAGGCTTGATTCCCAGCGGCGCTGTTTGGGCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCGGGTAGTCTGTGGCGCTTGCTGCAGGTTGACGTCCTGGCTGGGCAGCCCGACCCATTGCGGAAAACGCTGG  >  NC_002947/1487916‑1488213

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`