Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,549,801 C→T 9.7% G53G (GGC→GGT)  groL → chaperonin GroEL

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,549,8010CT 9.7% 1007.0 / 69.1 349G53G (GGC→GGT) groLchaperonin GroEL
Reads supporting (aligned to +/- strand):  ref base C (142/173);  new base T (20/14);  total (162/187)
Fisher's exact test for biased strand distribution p-value = 1.49e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01

Only 100 of 352 total aligned reads displayed.

AAAGACGTAAAATTTGGTGATTCCGCTCGTAAGAAAATGCTGGTTGGTGTCAACGTTCTGGCTGACGCGGTAAAAGCGACCCTGGGCCCGAAAGGCCGTAACGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGG  >  NC_002947/1549652‑1549950
                                                                                                                                                     |                                                                                                                                                     
      ttAAAATTTTTTTATTCCGCTCTAAATAAAATGCTGGTTGTTGTAAACGTTCTGGCTGACGCGTAAAAAGCGACCCTGGGCCCGAAAGGCCGTAACGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTcc                                                                                                                                                 <  1:2554376/149‑1 (MQ=255)
      gTAAAATTTGGTGATTCCGCTCGTAAGAAAATGCTGGTTGGTGTCAACGTTCTGGCTGACGCGGTAAAAGCGACCCTGGGCCCGAAAGGCCGTAACGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTcc                                                                                                                                                 >  1:1301564/1‑150 (MQ=255)
      gTAAAATTTGGTGATTCCGCTCGTAAGAAAATGCTGGTTGGTGTCAACGTTCTGGCTGACGCGGTAAAAGCGACCCTGGGCCCGAAAGGCCGTAACGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTcc                                                                                                                                                 >  1:6047097/1‑150 (MQ=255)
      gTAAAATTTGGTGATTCCGCTCGTAAGAAAATGCTGGTTGGTGTCAACGTTCTGGCTGACGCGGTAAAAGCGACCCTGGGCCCGAAAGGCCGTAACGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTcc                                                                                                                                                 >  1:3545369/1‑150 (MQ=255)
         aaaTTTGGTGATTCCGCTCGTAAGAAAATGCTGGTTGGTGTCAACGTTCTGGCTGACGCGGTAAAAGCGACCCTGGGCCCGAAAGGCCGTAACGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTc                                                                                                                                              >  1:7549537/1‑150 (MQ=255)
              tGGTGATTCCGCTCGTAAGAAAATGCTGGTTGGTGTCAACGTTCTGGCTGACGCGGTAAAAGCGACCCTGGGCCCGAAAGGCCGTAACGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCaa                                                                                                                                         <  2:5767797/150‑1 (MQ=255)
               ggTGATTCCGCTCGTAAGAAATTGCTGGTTGGTGTCAACGTTCTGGCTGACGCGGTAAAAGCGACCCTGGGCCCGAAAGGCCGTAACGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCaaa                                                                                                                                        <  1:8483999/150‑1 (MQ=255)
                  gATTCCGCTCGTAAGAAAATGCTGGTTGGTGTCAACGTTCTGGCTGACGCGGTAAAAGCGACCCTGGGCCCGAAAGGCCGTAACGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGaa                                                                                                                                     <  1:5647980/150‑1 (MQ=255)
                  gATTCCGCTCGTAAGAAAATGCTGGTTGGTGTCAACGTTCTGGCTGACGCGGTAAAAGCGACCCTGGGCCCGAAAGGCCGTAACGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGaa                                                                                                                                     >  1:2974699/1‑150 (MQ=255)
                  gATTCCGCTCGTAAGAAAATGCTGGTTGGTGTCAACGTTCTGGCTGACGCGGTAAAAGCGACCCTGGGCCCGAAAGGCCGTAACGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGaa                                                                                                                                     >  2:2422152/1‑150 (MQ=255)
                     tCCGCTCGTAAGAAAATGCTGGTTGGTGTCAACGTTCTGGCTGACGCGGTAAAAGCGACCCTGGGCCCGAAAGGCCGTAACGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATc                                                                                                                                  >  1:4420741/1‑150 (MQ=255)
                        gatCGTAATAAAATGCTGTTTGGTTTAAACGTTCTGGCTGACGCGTTAAAAGCGACCCTGGGCCCGAAAGGCCGTAACGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAg                                                                                                                               <  1:993902/148‑1 (MQ=255)
                        gCTCGTAAGAAAATGCTGGTTGGTGTCAACGTTCTGGCTGACGCGGTAAAAGCGACCCTGGGCCCGAAAGGCCGTAACGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAg                                                                                                                               <  1:5945983/150‑1 (MQ=255)
                             tAAGAAAATGCTGGTTGGTGTCAACGTTCTGGCTGACGCGGTAAAAGCGACCCTGGGCCCGAAAGGCCGTAACGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGaa                                                                                                                          >  1:2240481/1‑150 (MQ=255)
                              aaGAAAATGCTGGTTGGTGTCAACGTTCTGGCTGACGCGGTAAAAGCGACCCTGGGCCCGAAAGGCCGTAACGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGaaa                                                                                                                         <  2:4350587/150‑1 (MQ=255)
                               aGAAAATGCTGGTTGGTGTCAACGTTCTGGCTGACGCGGTAAAAGCGACCCTGGGCCCGAAAGGCCGTAACGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAg                                                                                                                        <  2:1627456/150‑1 (MQ=255)
                                      gCTGGTTGGTGTCAAGGTTCTGGCTGACGCGGTAAAAGCGACCCTGGGCCCGAAAGGCCGTAACGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCttt                                                                                                                 <  1:4646132/150‑1 (MQ=255)
                                      gCTGGTTGGTGTCAACGTTCTGGCTGACGCGGTAAAAGCGACCCTGGGCCCGAAAGGCCGTAACGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCttt                                                                                                                 <  2:4933037/150‑1 (MQ=255)
                                          gttggtGTCAACGTTCTGGCTGACGCGGTAAAAGCGACCCTGGGCCCGAAAGGCCGTAACGTGGTACTGGCCAATAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTACGaa                                                                                                             >  2:3264313/1‑150 (MQ=255)
                                          gttggtGTCAACGTTCTGGCTGACGCGGTAAAAGCGACCCTGGGCCCGAAAGGCCGTAACGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGaa                                                                                                             <  2:4273077/150‑1 (MQ=255)
                                          gttggtGTCAACGTTCTGGCTGACGCGGTAAAAGCGACCCTGGGCCCGAAAGGCCGTAACGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGaa                                                                                                             <  1:5328841/150‑1 (MQ=255)
                                           ttggtGTCAACGTTCTGGCTGACGCGGTAAAAGCGACCCTGGGCCCGAAAGGCCGTAACGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGaaa                                                                                                            >  1:6907331/1‑150 (MQ=255)
                                             ggtGTCAACGTTCTGGCTGACGCGGTAAAAGCGACCCTGGGCCCGAAAGGCCGTAACGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAAc                                                                                                          <  2:6496510/150‑1 (MQ=255)
                                                gtatactttCTGGCTGACGCTTTAAAAGCGAACCTGGGCCCGAAAGGCCGTAACGTTGTACTGGCCAAGAGCTTCGGCGCGCCGACCCTCACCAAGGAAGGCGTTTCCGTCGGCATAGAAATCGAGCTGAAAGACGCTTTCGAAAACATg                                                                                                       <  1:6550985/143‑1 (MQ=255)
                                                gtCAACGTTCTGGCTGACGCGGTAAAAGCGACCCTGGGCCCGAAAGGCCGTAACGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATg                                                                                                       <  2:7791263/150‑1 (MQ=255)
                                                gtCAACGTTCTGGCTGACGCGGTAAAAGCGACCCTGGGCCCGAAAGGCCGTAACGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATg                                                                                                       >  1:3299058/1‑150 (MQ=255)
                                                 tCAACTTTCTGGCTGACGCGGTAAAAGCGACCCTGGGCCCGAAAGGCCGTAACGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATgg                                                                                                      <  2:940018/150‑1 (MQ=255)
                                                 tCAACGTTCTGGCTGACGCGGTAAAAGCGACCCTGGGCCCGAAAGGCTGTAACGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATgg                                                                                                      >  2:376455/1‑150 (MQ=255)
                                                   aaCGTTCTGGCTGACGCGGTAAAAGCGACCCTGGGCCCGAAAGGCCGTAACGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGgc                                                                                                    >  2:1901704/1‑150 (MQ=255)
                                                   aaCGTTCTGGCTGACGCGGTAAAAGCGACCCTGGGCCCGAAAGGCCGTAACGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGgc                                                                                                    >  2:4889466/1‑150 (MQ=255)
                                                   aaCGTTCTGGCTGACGCGGTAAAAGCGACCCTGGGCCCGAAAGGCCGTAACGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGgc                                                                                                    >  2:7995267/1‑150 (MQ=255)
                                                   aaCGTTCTGGCTGACGCGGTAAAAGCGACCCTGGGCCCGAAAGGCCGTAACGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGgc                                                                                                    >  1:6712224/1‑150 (MQ=255)
                                                   aaCGTTCTGGCTGACGCGGTAAAAGCGACCCTGGGCCCGAAAGGCCGTAACGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCGGTCGAAAACATGGgc                                                                                                    >  1:718387/1‑150 (MQ=255)
                                                      gTTCTGGCTGACGCGGTAAAAGCGACCCTGGGCCCGAAAGGCCGTAACGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTc                                                                                                                                              >  1:2795930/1‑105 (MQ=255)
                                                           ggCTGACGCGGTAAAAGCGACCCTGGGCCCGAAAGGCCGTAACGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCt                                                                                            >  1:7416238/1‑150 (MQ=255)
                                                           ggCTGACGCGGTAAAAGCGACCCTGGGCCCGAAAGGCCGTAACGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCt                                                                                            >  1:140985/1‑150 (MQ=255)
                                                              tGACGCGGTAAAAGCGACCCTGGGCCCGAAAGGCCGTAACGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGt                                                                                         >  1:1505388/1‑150 (MQ=255)
                                                               gACGCGGTAAAAGCGACCCTGGGCCCGAAAGGCCGTAACGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTc                                                                                        <  2:7318355/150‑1 (MQ=255)
                                                                 cgcgGTAAAAGCGACCCTGGGCCCGAAAGGCCGTAACGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCaa                                                                                      <  2:7701939/150‑1 (MQ=255)
                                                                  gcgGTAAAAGCGACCCTGGGCCCGAAAGGCCGTAACGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAg                                                                                     <  2:5433860/150‑1 (MQ=255)
                                                                    ggTAAAAGCGACCCTGGGCCCGAAAGGCCGTAACGTGTTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGa                                                                                   <  1:7093237/150‑1 (MQ=255)
                                                                    ggTAAAAGCGACCCTGGGCCCGAAAGGCCGTAACGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGa                                                                                   >  2:2772874/1‑150 (MQ=255)
                                                                    ggTAAAAGCGACCCTGGGCCCGAAAGGCCGTAACGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGa                                                                                   <  1:1068850/150‑1 (MQ=255)
                                                                     gTAAAAGCGACCCTGGGCCCGAAAGGCCGTAACGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGaa                                                                                  <  2:8723361/150‑1 (MQ=255)
                                                                      tAAAAGCGACCCTGGGCCCGAAAGGCCGTAACGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAg                                                                                 >  2:6930509/1‑150 (MQ=255)
                                                                        aaaGCGACCCTGGGCCCGAAAGGCCGTAACGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGtt                                                                               >  2:266328/1‑150 (MQ=255)
                                                                        aaaGCGACCCTGGGCCCGAAAGGCCGTAACGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGtt                                                                               <  1:7743671/150‑1 (MQ=255)
                                                                         aaGCGACCCTGGGCCCGAAAGGCCGTAACGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTg                                                                              >  1:2050342/1‑150 (MQ=255)
                                                                          aGCGACCCTGGGCCCGAAAGGCCGTAACGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGa                                                                                                              <  1:8348432/117‑1 (MQ=255)
                                                                          aGCGACCCTGGGCCCGAAAGGCCGTAACGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGa                                                                                                              >  2:8348432/1‑117 (MQ=255)
                                                                          aGCGACCCTGGGCCCGAAAGGCCGTAACGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGc                                                                             >  1:1921103/1‑150 (MQ=255)
                                                                            cGACCCTGGGCCCGAAAGGCCGTAACGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCtt                                                                           <  1:8577158/150‑1 (MQ=255)
                                                                                ccTGGGCCCGAAAGGCCGTAACGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCaa                                                                       <  1:8166319/150‑1 (MQ=255)
                                                                                 cTGGGCCCGAAAGGCCGTAACGTGGTACTGGCCATGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCCAAGAAATCGAGCTGAAAGACGCTTTCGAAATCATGGGCGCCCAGCTGGTCAAGGCAGTTGCTTCCaat                                                                      <  2:671797/150‑2 (MQ=255)
                                                                                 cTGGGCCCGAAAGGCCGTAACGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAg                                                                      <  2:3556293/150‑1 (MQ=255)
                                                                                        cGAAAGGCCGTAACGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAacg                                                               >  1:2377164/1‑150 (MQ=255)
                                                                                         gAAAGGCCGTAACGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAAGATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAacga                                                              <  2:8184803/150‑1 (MQ=255)
                                                                                               ccGTAACGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGc                                                        <  2:1719335/150‑1 (MQ=255)
                                                                                                   aaCGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGt                                                    <  2:7849275/150‑1 (MQ=255)
                                                                                                     cGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGa                                                  <  2:2619559/150‑1 (MQ=255)
                                                                                                     cGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGa                                                  <  2:8498258/150‑1 (MQ=255)
                                                                                                        gttACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACgg                                               <  1:5570938/148‑1 (MQ=255)
                                                                                                          tACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGca                                             >  2:6375212/1‑150 (MQ=255)
                                                                                                          tACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGca                                             <  2:8873020/150‑1 (MQ=255)
                                                                                                           aCTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGcac                                            >  1:4046825/1‑150 (MQ=255)
                                                                                                               gCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGcaccacc                                        >  1:8677030/1‑150 (MQ=255)
                                                                                                                ccAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGcaccacca                                       >  2:6117209/1‑150 (MQ=255)
                                                                                                                 cAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGcaccaccac                                      >  2:1078144/1‑150 (MQ=255)
                                                                                                                 cAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACAGGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGcaccaccac                                      <  2:4612585/150‑1 (MQ=255)
                                                                                                                  aaGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCaccaccacc                                     >  2:2290210/1‑150 (MQ=255)
                                                                                                                   agagCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCg                                    >  1:5735667/1‑150 (MQ=255)
                                                                                                                       cTTGGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAATGAAATCGCGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTAc                                <  1:7951955/150‑1 (MQ=255)
                                                                                                                        ttCGGCGCTCCACGCATCACCAAGGACGGTGTATCGGTTGCCAAGGAAATCGAACTGGAAGACAAGTTCGAAAACATGGGCGCACAGATGCTGCGCGAAGTGGCTTCGAAGACCAACGACACTGCCGGTGACGGCACCACCACTGCGAcc                               >  1:6309776/1‑150 (MQ=255)
                                                                                                                        ttCGGCGCTCCACGCATCACCAAGGACGGTGTATCGGTTGCCAAGGAAATCGAACTGGAAGACAAGTTCGAAAACATGGGCGCACAGATGCTGCGCGAAGTGGCTTCGAAGACCAACGACACTGCCGGTGACGGCACCACCACTGCGAcc                               >  2:6630208/1‑150 (MQ=255)
                                                                                                                        ttCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCCAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTAcc                               >  2:5507970/1‑150 (MQ=255)
                                                                                                                             cgcgcCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCt                          <  2:1209192/150‑1 (MQ=255)
                                                                                                                              gcgcCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTg                         <  2:3691444/150‑1 (MQ=255)
                                                                                                                               cgcCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTgg                        <  1:4219214/150‑1 (MQ=255)
                                                                                                                               cgcCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTgg                        <  1:4168424/150‑1 (MQ=255)
                                                                                                                                gcCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGc                       >  1:6422302/1‑150 (MQ=255)
                                                                                                                                 ccGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCt                      >  1:3919263/1‑150 (MQ=255)
                                                                                                                                      cATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGc                 >  1:1952884/1‑150 (MQ=255)
                                                                                                                                         cACCAAGGACGGTGTATCGGTTGCCAAGGAAATCGAACTGGAAGACAAGTTCGAAAACATGGGCGCACAGATGCTGCGCGAAGTGGCTTCGAAGACCAACGACACTGCCGGTGACGGCACCACCACTGCGACCGTTCTCGGTCAGGCTAt              >  1:449417/1‑150 (MQ=255)
                                                                                                                                         cACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCAt              <  1:5074130/150‑1 (MQ=255)
                                                                                                                                          aCCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATc             <  2:2801746/150‑1 (MQ=255)
                                                                                                                                            cAAGGACGGTGTATCGGTTGCCAAGGAAATCGAACTGGAAGACAAGTTCGAAAACATGGGCGCACAGATGCTGCGCGAAGTGGCTTCGAAGACCAACGACACTGCCGGTGACGGCACCACCACTGCGACCGTTCTCGGTCAGGCTATCGt           >  1:8740938/1‑150 (MQ=255)
                                                                                                                                            cAAGGACGGTGTATCGGTTGCCAAGGAAATCGAACTGGAAGACAAGTTCGAAAACATGGGCGCACAGATGCTGCGCGAAGTGGCTTCGAAGACCAACGACACTGCCGGTGACGGCACCACCACTGCGACCGTTCTCGGTCAGGCTATCGt           >  2:8609471/1‑150 (MQ=255)
                                                                                                                                            cAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGt           <  1:8774026/150‑1 (MQ=255)
                                                                                                                                            cAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGt           >  2:2929410/1‑150 (MQ=255)
                                                                                                                                            cAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGt           <  2:7165790/150‑1 (MQ=255)
                                                                                                                                            cAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGt           >  2:3442859/1‑150 (MQ=255)
                                                                                                                                             aaGGACGGTGTATCGGTTGCCAAGGAAATCGAACTGGAAGACAAGTTCGAAAACATGGGCGCACAGATGCTGCGCGAAGTGGCTTCGAAGACCAACGACACTGCCGGTGACGGCACCACCACTGCGACCGTTCTCGGTCAGGCTATCGtt          <  1:8368941/150‑2 (MQ=255)
                                                                                                                                             aaGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTc          >  2:7425424/1‑150 (MQ=255)
                                                                                                                                              aGGACGGTGTATCGGTTGCCAAGGAAATCGAACTGGAAGACACGTTCGAAAACATGGGCGCACAGATGCTGCGCGAAGTGGCTTCGAAGACCAACGACACTGCCGGTGACGGCACCACCACTGCGACCGTTCTCGGTCAGGCTATCGttc         <  1:4821067/150‑3 (MQ=255)
                                                                                                                                               ggACGGTGTATCGGTTGCCAAGGAAATCGAACTGGAAGACAAGTTCGAAAACATGGGCGCACAGATGCTGCGCGAAGTGGCTTCGAAGACCAACGACACTGCCGGTGACGGCACCACCACTGCGACCGTTCTCGGTCAGGCTATCGttca        <  2:3828326/150‑4 (MQ=255)
                                                                                                                                               ggACGGTGTATCGGTTGCCAAGGAAATCGAACTGGAAGACAAGTTCGAAAACATGGGCGCACAGATGCTGCGCGAAGTGGCTTCGAAGACCAACGACACTGCCGGTGACGGCACCACCACTGCGACCGTTCTCGGTCAGGCTATCGttca        <  1:3104865/150‑4 (MQ=255)
                                                                                                                                                gACGGTGTATCGGTTGCCAAGGAAATCGAACTGGAAGACAAGTTCGAAAACATGGGCGCACAGATGCTGCGCGAAGTGGCTTCGAAGACCAACGACACTGCCGGTGACGGCACCACCACTGCGACCGTTCTCGGTCAGGCTATCGttcag       <  2:1537551/150‑5 (MQ=255)
                                                                                                                                                gACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAAc       <  1:2158011/150‑1 (MQ=255)
                                                                                                                                                   ggCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGaa    <  1:9165823/150‑1 (MQ=255)
                                                                                                                                                   ggCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGaa    <  2:4087666/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
AAAGACGTAAAATTTGGTGATTCCGCTCGTAAGAAAATGCTGGTTGGTGTCAACGTTCTGGCTGACGCGGTAAAAGCGACCCTGGGCCCGAAAGGCCGTAACGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGG  >  NC_002947/1549652‑1549950

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑