BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,549,864	C→G	14.2%	V74V (GTC→GTG) ‡	groL →	chaperonin GroEL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,549,864	0	C	G	14.2%	1009.0 / 136.3	352	V74V (GTC→GTG) ‡	groL	chaperonin GroEL
Reads supporting (aligned to +/- strand): ref base C (130/172); new base G (29/21); total (159/193)
Fisher's exact test for biased strand distribution p-value = 6.49e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01

Only 100 of 355 total aligned reads displayed.

GACGCGGTAAAAGCGACCCTGGGCCCGAAAGGCCGTAACGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCAC  >  NC_002947/1549715‑1550013

  cgcgGTAAAAGCGACCCTGGGCCCGAAAGGCCGTAACGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCaa                                                                                                                                                     <  2:2748822/150‑1 (MQ=255)

     ggTAAAAGCGACCCTGGGCCCGAAAGGCCGTAACGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGa                                                                                                                                                  <  1:1068850/150‑1 (MQ=255)

     ggTAAAAGCGACCCTGGGCCCGAAAGGCCGTAACGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGa                                                                                                                                                  <  1:7093295/150‑1 (MQ=255)

      gTAATAGCGACCCTGGGCCCGAAAGGCCGTAACGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGaa                                                                                                                                                 <  1:8365601/150‑1 (MQ=255)

      gTAAAAGCGACCCTGGGCCCGAAAGGCCGTAACGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGaa                                                                                                                                                 <  2:8723361/150‑1 (MQ=255)

         aaaGCGACCCTGGGCCCGAAAGGCCGTAACGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGtt                                                                                                                                              <  1:7743671/150‑1 (MQ=255)

         aaaGCGACCCTGGGCCCGAAAGGCCGTAACGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGtt                                                                                                                                              >  2:266328/1‑150 (MQ=255)

            gCGACCCTGGGCCCGAAAGGCCGTAACGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCt                                                                                                                                           >  2:7021143/1‑150 (MQ=255)

               aCCCTGGGCCCGAAAGGCCGTAACGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAATCATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTcc                                                                                                                                        <  2:6728574/150‑1 (MQ=255)

                 ccTGGGCCCGAAAGGCCGTAACGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCaa                                                                                                                                      <  1:8166319/150‑1 (MQ=255)

                 ccTGGGCCCGAAAGGCCGTAACGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCaa                                                                                                                                      <  1:8815243/150‑1 (MQ=255)

                  cTGGGCCCGAAAGGCCGTAACGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAg                                                                                                                                     <  2:3556293/150‑1 (MQ=255)

                   tGGGCCCGAAAGGCCGTAACGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAgg                                                                                                                                    <  1:2014159/150‑1 (MQ=255)

                   tGGGCCCGAAAGGCCGTAACGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAgg                                                                                                                                    >  2:6900023/1‑150 (MQ=255)

                      gCCCGAAAGGCCGTAACGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCa                                                                                                                                 >  2:7880973/1‑150 (MQ=255)

                        ccGAAAGGCCGTAACGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAac                                                                                                                               <  1:8707309/150‑1 (MQ=255)

                          gAAAGGCCGTAACGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAAGATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAacga                                                                                                                             <  2:8184803/150‑1 (MQ=255)

                                ccGTAACGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGc                                                                                                                       >  1:1949767/1‑150 (MQ=255)

                                    aaCGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGt                                                                                                                   <  2:7849235/150‑1 (MQ=255)

                                         ggTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACgg                                                                                                              <  1:5571025/150‑1 (MQ=255)

                                           tACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGca                                                                                                            <  2:8873020/150‑1 (MQ=255)

                                           tACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGca                                                                                                            >  2:6375212/1‑150 (MQ=255)

                                            aCTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGcac                                                                                                           <  1:442040/150‑1 (MQ=255)

                                            aCTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGcac                                                                                                           <  2:1575597/150‑1 (MQ=255)

                                            aCTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGcac                                                                                                           <  1:6850464/150‑1 (MQ=255)

                                            aCTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGcac                                                                                                           <  1:2227232/150‑1 (MQ=255)

                                              tGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGcacca                                                                                                         >  1:3033904/1‑150 (MQ=255)

                                                gCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGcaccacc                                                                                                       >  2:2574393/1‑150 (MQ=255)

                                                  cAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGcaccaccac                                                                                                     <  1:4539818/150‑1 (MQ=255)

                                                  cAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACAGGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGcaccaccac                                                                                                     <  2:4612585/150‑1 (MQ=255)

                                                   aaGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCaccaccacc                                                                                                    >  1:7466227/1‑150 (MQ=255)

                                                   aaGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCaccaccacc                                                                                                    >  2:2290210/1‑150 (MQ=255)

                                                    agtccTTCGGCGCTCCACGCATCACCAAGGACGGTGTATCGGTTGCCAAGGAAATCGAACTGGAAGACAAGTTCGAAAACATGGGCGCACAGATGCTGCGCGAAGTGGCTTCGAAGACCAACGACACTGCCGGTGACGGcaccaccactg                                                                                                   <  2:3611560/146‑3 (MQ=255)

                                                    agagCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCg                                                                                                   >  2:1189085/1‑150 (MQ=255)

                                                        cTTGGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAATGAAATCGCGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTAc                                                                                               <  1:7951955/150‑1 (MQ=255)

                                                        cTTCGGCGCTCCACGCATCACCAAGGACGGTGTATCGGTTGCCAAGGAAATCGAACTGGAAGACAAGTTCGAAAACATGGGCGCACAGATGCTGCGCGAAGTGGCTTCGAAGACCAACGACACTGCCGGTGACGGCACCACCACTGCGAc                                                                                               >  2:22174/1‑150 (MQ=255)

                                                         ttCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCCAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTAcc                                                                                              >  2:5507970/1‑150 (MQ=255)

                                                             gcgcgcCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTc                                                                                          <  2:7322791/150‑1 (MQ=255)

                                                             gcgcgcCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTc                                                                                          <  1:1350752/150‑1 (MQ=255)

                                                              cgcgcCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCt                                                                                         <  2:500618/150‑1 (MQ=255)

                                                              cgcgcCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCt                                                                                         <  2:6551694/150‑1 (MQ=255)

                                                               gcgcCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTg                                                                                        <  2:3691444/150‑1 (MQ=255)

                                                               gcgcCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTg                                                                                        <  1:391720/150‑1 (MQ=255)

                                                                cgcCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTgg                                                                                       <  1:4168618/150‑1 (MQ=255)

                                                                  ccGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCt                                                                                     <  1:4117675/150‑1 (MQ=255)

                                                                     aCCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAg                                                                                  <  2:6010204/150‑1 (MQ=255)

                                                                      gcATCACCAAGGACGGTGTATCGGTTGCCAAGGAAATCGAACTGGAAGACAAGTTCGAAAACATGGGCGCACAGATGCTGCGCGAAGTGGCTTCGAAGACCAACGACACTGCCGGTGACGGCACCACCACTGCGACCGTTCTCGGTCAgg                                                                                 <  2:3824054/149‑1 (MQ=255)

                                                                       cATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGc                                                                                >  1:8414004/1‑150 (MQ=255)

                                                                          cACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCAt                                                                             <  1:5074130/150‑1 (MQ=255)

                                                                           aCCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGAGGCTTTCGAAATCTTGGGCGGCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCTCCGCTACCGTTCTGGCTCAGGCCATc                                                                            >  2:169669/1‑150 (MQ=255)

                                                                           aCCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATc                                                                            >  1:6338255/1‑150 (MQ=255)

                                                                            ccAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCg                                                                           <  1:566023/150‑1 (MQ=255)

                                                                             cAAGGACGGTGTATCGGTTGCCAAGGAAATCGAACTGGAAGACAAGTTCGAAAACATGGGCGCACAGATGCTGCGCGAAGTGGCTTCGAAGACCAACGACACTGCCGGTGACGGCACCACCACTGCGACCGTTCTCGGTCAGGCTATCGt                                                                          >  1:8741012/1‑150 (MQ=255)

                                                                             cAAGGACGGTGTATCGGTTGCCAAGGAAATCGAACTGGAAGACAAGTTCGAAAACATGGGCGCACAGATGCTGCGCGAAGTGGCTTCGAAGACCAACGACACTGCCGGTGACGGCACCACCACTGCGACCGTTCTCGGTCAGGCTATCGt                                                                          >  2:3356584/1‑150 (MQ=255)

                                                                             cAAGGACGGTGTATCGGTTGCCAAGGAAATCGAACTGGAAGACAAGTTCGAAAACATGGGCGCACAGATGCTGCGCGAAGTGGCTTCGAAGACCAACGACACTGCCGGTGACGGCACCACCACTGCGACCGTTCTCGGTCAGGCTATCGt                                                                          >  2:8609471/1‑150 (MQ=255)

                                                                             cAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGt                                                                          <  2:7165790/150‑1 (MQ=255)

                                                                             cAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGt                                                                          >  2:2929410/1‑150 (MQ=255)

                                                                              aaGGACGGTGTATCGGTTGCCAAGGAAATCGAACTGGAAGACAAGTTCGAAAACATGGGCGCACAGATGCTGCGCGAAGTGGCTTCGAAGACCAACGACACTGCCGGTGACGGCACCACCACTGCGACCGTTCTCGGTCAGGCTATCGtt                                                                         >  1:8634929/1‑149 (MQ=255)

                                                                              aaGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTc                                                                         <  1:5772456/150‑1 (MQ=255)

                                                                               aGGACGGTGTATCGGTTGCCAAGGAAATCGAACTGGAAGACACGTTCGAAAACATGGGCGCACAGATGCTGCGCGAAGTGGCTTCGAAGACCAACGACACTGCCGGTGACGGCACCACCACTGCGACCGTTCTCGGTCAGGCTATCGttc                                                                        <  1:4821067/150‑3 (MQ=255)

                                                                                ggACGGTGTATCGGTTGCCAAGGAAATCGAACTGGAAGACAAGTTCGAAAACATGGGCGCACAGATGCTGCGCGAAGTGGCTTCGAAGACCAACGACACTGCCGGTGACGGCACCACCACTGCGACCGTTCTCGGTCAGGCTATCGttca                                                                       <  1:3104865/150‑4 (MQ=255)

                                                                                 gACGGTGTATCGGTTGCCAAGGAAATCGAACTGGAAGACAAGTTCGAAAACATGGGCGCACAGATGCTGCGCGAAGTGGCTTCGAAGACCAACGACACTGCCGGTGACGGCACCACCACTGCGACCGTTCTCGGTCAGGCTATCGttcag                                                                      >  2:3885330/1‑146 (MQ=255)

                                                                                 gACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAAc                                                                      <  1:2158011/150‑1 (MQ=255)

                                                                                    ggCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGaa                                                                   <  1:9165823/150‑1 (MQ=255)

                                                                                        tttCCGTCGCCAAAGAAATCGAGCTGAAAGACGCGTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCTCCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGcc                                                               <  1:2803413/150‑1 (MQ=255)

                                                                                                 ccAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTg                                                                                                                                             >  2:4349809/1‑63 (MQ=255)

                                                                                                  cAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGt                                                     <  1:663989/150‑1 (MQ=255)

                                                                                                  cAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGt                                                     >  2:279513/1‑150 (MQ=255)

                                                                                                   aaaGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTc                                                    >  1:5365489/1‑150 (MQ=255)

                                                                                                   aaaGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTc                                                    >  2:5359379/1‑150 (MQ=255)

                                                                                                   aaaGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTc                                                    >  2:2169093/1‑150 (MQ=255)

                                                                                                    aaGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGa                                                                    <  2:8363192/133‑1 (MQ=255)

                                                                                                      gAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCGAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCt                                                 <  1:8561420/150‑1 (MQ=255)

                                                                                                      gAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCt                                                 >  2:84698/1‑150 (MQ=255)

                                                                                                        aaTCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGc                                               <  1:7786658/150‑1 (MQ=255)

                                                                                                                     gACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCg                                  >  2:3459773/1‑150 (MQ=255)

                                                                                                                     gACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCg                                  >  1:1510515/1‑150 (MQ=255)

                                                                                                                       cGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGAt                                <  1:2684609/150‑1 (MQ=255)

                                                                                                                        gCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATg                               <  1:1787049/150‑1 (MQ=255)

                                                                                                                        aagttCGAAAACATGGGCGCACAGATGCTGCGCGAAGTGGCTTCGAAGACCAACGACACTGCCGGTGACGGCACCACCACTGCGACCGTTCTCGGTCAGGCTATCGTTCAGGAAGGCGCCAAGGCTGTTGCCGCTGGCATGAACCCAATg                               <  2:2373461/147‑1 (MQ=255)

                                                                                                                        aagttCGAAAACATGGGCGCACAGATGCTGCGCGAAGTGGCTTCGAAGACCAACGACACTGCCGGTGACGGCACCACCACTGCGACCGTTCTCGGTCAGGCTATCGTTCAGGAAGGCGCCAAGGCTGTTGCCGCTGGCATGAACCCAATg                               >  1:1643053/4‑150 (MQ=255)

                                                                                                                        aagttCGAAAACATGGGCGCACAGATGCTGCGCGAAGTGGCTTCGAAGACCAACGACACTGCCGGTGACGGCACCACCACTGCGACCGTTCTCGGTCAGGCTATCGTTCAGGAAGGCGCCAAGGCTGTTGCCGCTGGCATGAACCCAATg                               <  2:1358880/147‑1 (MQ=255)

                                                                                                                         agttCGAAAACATGGGCGCACAGATGCTGCGCGAAGTGGCTTCGAAGACCAACGACACTGCCGGTGACGGCACCACCACTGCGATCGTTCTCGGTCAGGCTATCGTTCAGGAAGGCGCCAAGGCTGTTGCCGCTGGCATGAACCCAATgg                              <  1:5525267/148‑1 (MQ=255)

                                                                                                                           ttCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGAc                            >  2:5926219/1‑150 (MQ=255)

                                                                                                                           ttCGAAAACATGGGCGCACAGATGCTGCGCGAAGTGGCTTCGAAGACCAACGACACTGCCGGTGACGGCACCACCACTGCGACCGTTCTCGGTCAGGCTATCGTTCAGGAAGGCGCCAAGGCTGTTGCCGCTGGCATGAACCCAATGGAc                            >  1:432349/1‑150 (MQ=255)

                                                                                                                           ttCGAAAACATGGGCGCACAGATGCTGCGCGAAGTGGCTTCGAAGACCAACGACACTGCCGGTGACGGCACCACCACTGCGACCGTTCTCGGTCAGGCTATCGTTCAGGAAGGCGCCAAGGCTGTTGCCGCTGGCATGAACCCAATGGAc                            <  1:6581583/150‑1 (MQ=255)

                                                                                                                            tCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGAcc                           >  1:5975878/1‑150 (MQ=255)

                                                                                                                              gAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTg                         >  2:9123339/1‑150 (MQ=255)

                                                                                                                              gAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTg                         >  1:1891319/1‑150 (MQ=255)

                                                                                                                               aaaaCATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGa                        >  1:643960/1‑150 (MQ=255)

                                                                                                                                  aCATGGGCGCACAGATGCTGCGCGAAGTGGCTTCGAAGACCAACGACACTGCCGGTGACGGCACCACCACTGCGACCGTTCTCGGTCAGGCTATCGTTCAGGAAGGCGCCAAGGCTGTTGCCGCTGGCATGAACCCAATGGACCTGAAgc                     >  1:5723795/1‑150 (MQ=255)

                                                                                                                                   cATGGGCGCCCAGCTGGTCAAGGAAGCTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAgcg                    >  2:7587735/1‑150 (MQ=255)

                                                                                                                                    aTGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAgcgc                   <  2:4505030/150‑1 (MQ=255)

                                                                                                                                     tGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGcgcg                  <  1:2422152/150‑1 (MQ=255)

                                                                                                                                     tGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGcgcg                  <  2:3224800/150‑1 (MQ=255)

                                                                                                                                          gcCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATc             >  2:7263256/1‑150 (MQ=255)

                                                                                                                                            ccAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGa           >  2:132797/1‑150 (MQ=255)

                                                                                                                                            ccAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGa           >  2:3940717/1‑150 (MQ=255)

                                                                                                                                                    tCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCa   <  2:4275412/150‑1 (MQ=255)

                                                                                                                                                     cAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCAc  >  1:3528432/1‑150 (MQ=255)

GACGCGGTAAAAGCGACCCTGGGCCCGAAAGGCCGTAACGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCAC  >  NC_002947/1549715‑1550013

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`