BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,549,865	A→C	14.6%	K75Q (AAG→CAG) ‡	groL →	chaperonin GroEL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,549,865	0	A	C	14.6%	957.6 / 137.0	348	K75Q (AAG→CAG) ‡	groL	chaperonin GroEL
Reads supporting (aligned to +/- strand): ref base A (131/166); new base C (29/21); total (160/188)
Fisher's exact test for biased strand distribution p-value = 9.10e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.55e-01

Only 100 of 362 total aligned reads displayed.

CGCGGTAAAAGCGACCCTGGGCCCGAAAGGCCGTAACGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACT  >  NC_002947/1549717‑1550014

cgcgGTAAAAGCGACCCTGGGCCCGAAAGGCCGTAACGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCaa                                                                                                                                                      <  1:5911193/150‑1 (MQ=255)

cgcgGTAAAAGCGACCCTGGGCCCGAAAGGCCGTAACGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCaa                                                                                                                                                      <  2:7701939/150‑1 (MQ=255)

   ggTAAAAGCGACCCTGGGCCCGAAAGGCCGTAACGTGTTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGa                                                                                                                                                   <  1:7093237/150‑1 (MQ=255)

   ggTAAAAGCGACCCTGGGCCCGAAAGGCCGTAACGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGa                                                                                                                                                   <  1:1068850/150‑1 (MQ=255)

    gTAAAAGCGACCCTGGGCCCGAAAGGCCGTAACGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGaa                                                                                                                                                  <  2:8723361/150‑1 (MQ=255)

      aaaaGCGACCCTGGGCCCGAAAGGCCGTAACGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGt                                                                                                                                                <  1:6232762/150‑1 (MQ=255)

       aaaGCGACCCTGGGCCCGAAAGGCCGTAACGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGtt                                                                                                                                               <  1:3126734/150‑1 (MQ=255)

        aaGCGACCCTGGGCCCGAAAGGCCGTAACGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTg                                                                                                                                              >  1:2050087/1‑150 (MQ=255)

        aaGCGACCCTGGGCCCGAAAGGCCGTAACGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTg                                                                                                                                              >  2:2621064/1‑150 (MQ=255)

         aGCGACCCTGGGCCCGAAAGGCCGTAACGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGc                                                                                                                                             >  2:4230021/1‑150 (MQ=255)

          gCGACCCTGGGCCCGAAAGGCCGTAACGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCt                                                                                                                                            <  2:268583/150‑1 (MQ=255)

          gCGACCCTGGGCCCGAAAGGCCGTAACGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCt                                                                                                                                            >  2:7021143/1‑150 (MQ=255)

           cGACCCTGGGCCCGAAAGGCCGTAACGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCtt                                                                                                                                           <  1:8577158/150‑1 (MQ=255)

            gACCCTGGGCCCGAAAGGCCGTAACGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTc                                                                                                                                          <  1:8182006/150‑1 (MQ=255)

             aCCCTGGGCCCGAAAGGCCGTAACGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAATCATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTcc                                                                                                                                         <  2:6728574/150‑1 (MQ=255)

               ccTGGGCCCGAAAGGCCGTAACGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCaa                                                                                                                                       <  2:3797357/150‑1 (MQ=255)

                 tGGGCCCGAAAGGCCGTAACGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAgg                                                                                                                                     >  2:6900023/1‑150 (MQ=255)

                       cGAAAGGCCGTAACGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAacg                                                                                                                               >  1:2376798/1‑150 (MQ=255)

                             gCCGTAACGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTg                                                                                                                         <  1:8756827/150‑1 (MQ=255)

                               cGTAACGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAacgacg                                                                                                                            >  1:8707930/1‑145 (MQ=255)

                                  aaCGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGt                                                                                                                    <  2:7849275/150‑1 (MQ=255)

                                         tACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGca                                                                                                             <  2:8873020/150‑1 (MQ=255)

                                          aCTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGcac                                                                                                            <  2:5506874/150‑1 (MQ=255)

                                              gCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGcaccacc                                                                                                        >  1:8676202/1‑150 (MQ=255)

                                                cAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGcaccaccac                                                                                                      <  2:2509539/150‑1 (MQ=255)

                                                cAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGcaccaccac                                                                                                      <  2:7545295/150‑1 (MQ=255)

                                                cAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACAGGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGcaccaccac                                                                                                      <  2:4612585/150‑1 (MQ=255)

                                                 aaGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCaccaccacc                                                                                                     >  1:7772785/1‑150 (MQ=255)

                                                  agagCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCg                                                                                                    >  2:1189085/1‑150 (MQ=255)

                                                   gtccTTCGGCGCTCCACGCATCACCAAGGACGGTGTATCGGTTGCCAAGGAAATCGAACTGGAAGACAAGTTCGAAAACATGGGCGCACAGATGCTGCGCGAAGTGGCTTCGAAGACCAACGACACTGCCGGTGACGGCACCACCACTGc                                                                                                   >  2:2323356/4‑150 (MQ=255)

                                                    tccTTCGGCGCTCCACGCATCACCAAGGACGGTGTATCGGTTGCCAAGGAAATCGAACTGGAAGACAAGTTCGAAAACATGGGCGCACAGATGCTGCGCGAAGTGGCTTCGAAGACCAACGACACTGCCGGTGACGGCACCACCACTGcg                                                                                                  >  2:3391931/3‑149 (MQ=255)

                                                     ccTTCGGCGCTCCACGCATCACCAAGGACGGTGTATCGGTTGCCAAGGAAATCGAACTGGAAGACAAGTTCGAAAACATGGGCGCACAGATGCTGCGCGAAGTGGCTTCGAAGACCAACGACACTGCCGGTGACGGCACCACCACTGcga                                                                                                 <  1:5627640/149‑3 (MQ=255)

                                                       ttCGGCGCTCCACGCATCACCAAGGACGGTGTATCGGTTGCCAAGGAAATCGAACTGGAAGACAAGTTCGAAAACATGGGCGCACAGATGCTGCGCGAAGTGGCTTCGAAGACCAACGACACTGCCGGTGACGGCACCACCACTGCGAcc                                                                                               <  1:5713107/150‑1 (MQ=255)

                                                       ttCGGCGCTCCACGCATCACCAAGGACGGTGTATCGGTTGCCAAGGAAATCGAACTGGAAGACAAGTTCGAAAACATGGGCGCACAGATGCTGCGCGAAGTGGCTTCGAAGACCAACGACACTGCCGGTGACGGCACCACCACTGCGAcc                                                                                               <  2:4897482/150‑1 (MQ=255)

                                                           gcgcgcCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTc                                                                                           <  1:1350752/150‑1 (MQ=255)

                                                           gcgcgcCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTc                                                                                           <  1:1913594/150‑1 (MQ=255)

                                                           gcgcgcCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACAGGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTc                                                                                           <  1:5541172/150‑1 (MQ=255)

                                                            cgcgcCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCt                                                                                          <  1:8045625/150‑1 (MQ=255)

                                                            cgcgcCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCt                                                                                          <  2:6551694/150‑1 (MQ=255)

                                                              cgcCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTgg                                                                                        <  1:4168392/150‑1 (MQ=255)

                                                               gcCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGc                                                                                       >  2:3030400/1‑150 (MQ=255)

                                                               gcCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGc                                                                                       >  1:6422302/1‑150 (MQ=255)

                                                                ccGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCt                                                                                      >  1:3919263/1‑150 (MQ=255)

                                                                  gACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCa                                                                                    <  2:6276826/150‑1 (MQ=255)

                                                                   aCCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAg                                                                                   <  2:6010204/150‑1 (MQ=255)

                                                                     cATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGc                                                                                 >  1:8414097/1‑150 (MQ=255)

                                                                     cATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGc                                                                                 >  1:7878930/1‑150 (MQ=255)

                                                                     cATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGc                                                                                 >  1:1952884/1‑150 (MQ=255)

                                                                         aCCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGAGGCTTTCGAAATCTTGGGCGGCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCTCCGCTACCGTTCTGGCTCAGGCCATc                                                                             >  2:169669/1‑150 (MQ=255)

                                                                         aCCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATc                                                                             >  1:8919293/1‑150 (MQ=255)

                                                                           cAAGGACGGTGTATCGGTTGCCAAGGAAATCGAACTGGAAGACAAGTTCGAAAACATGGGCGCACAGATGCTGCGCGAAGTGGCTTCGAAGACCAACGACACTGCCGGTGACGGCACCACCACTGCGACCGTTCTCGGTCAGGCTATCGt                                                                           >  2:8609471/1‑150 (MQ=255)

                                                                           cAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGt                                                                           >  2:2784528/1‑150 (MQ=255)

                                                                           cAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGt                                                                           >  1:4476050/1‑150 (MQ=255)

                                                                           cAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGt                                                                           <  2:7165790/150‑1 (MQ=255)

                                                                            aaGGACGGTGTATCGGTTGCCAAGGAAATCGAACTGGAAGACAAGTTCGAAAACATGGGCGCACAGATGCTGCGCGAAGTGGCTTCGAAGACCAACGACACTGCCGGTGACGGCACCACCACTGCGACCGTTCTCGGTCAGGCTATCGtt                                                                          <  1:8368941/150‑2 (MQ=255)

                                                                              ggACGGTGTATCGGTTGCCAAGGAAATCGAACTGGAAGACAAGTTCGAAAACATGGGCGCACAGATGCTGCGCGAAGTGGCTTCGAAGACCAACGACACTGCCGGTGACGGCACCACCACTGCGACCGTTCTCGGTCAGGCTATCGttca                                                                        <  2:2808804/150‑4 (MQ=255)

                                                                               gACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAAc                                                                       <  2:3545369/150‑1 (MQ=255)

                                                                                  ggCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGaa                                                                    <  1:3538322/150‑1 (MQ=255)

                                                                                  ggCGTTTCCGTCGCCAAAGAAATCCAGCTGAAAGACGCATTCGAAAACATCGGCGCCCAGCTGGTCAAGGAAGATGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGATCTGGCTCAGCCCATCGACAACCaa                                                                    >  2:4345834/1‑150 (MQ=255)

                                                                                    cGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAgg                                                                  <  1:6590220/150‑1 (MQ=255)

                                                                                         ccGTCGCCAAAGAAATCGAGCTGAAAGACGCTATCGAAAACATGCGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGCCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAACGCCTGa                                                             <  2:8035503/150‑1 (MQ=255)

                                                                                           gTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCTTCGTTGACGATGGCCTGagc                                                           <  1:2907709/150‑3 (MQ=255)

                                                                                           gTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGaaa                                                           <  2:4483883/150‑1 (MQ=255)

                                                                                              gCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCg                                                                                                                      >  1:5752918/1‑88 (MQ=255)

                                                                                              gCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCg                                                                                                                      <  2:5752918/88‑1 (MQ=255)

                                                                                               ccAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTg                                                                                                                                              <  1:4349809/63‑1 (MQ=255)

                                                                                               ccAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCg                                                       >  2:1541422/1‑150 (MQ=255)

                                                                                                 aaaGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTc                                                     >  2:5359379/1‑150 (MQ=255)

                                                                                                 aaaGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTc                                                     >  2:3676190/1‑150 (MQ=255)

                                                                                                  aaGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGa                                                                     <  2:8363192/133‑1 (MQ=255)

                                                                                                   aGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGc                                                   >  1:5379934/1‑150 (MQ=255)

                                                                                                    gAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCt                                                  >  2:84424/1‑150 (MQ=255)

                                                                                                      aaTCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTgg                                                                                        <  2:3308032/110‑1 (MQ=255)

                                                                                                         cGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCgg                                             >  1:3040656/1‑150 (MQ=255)

                                                                                                            gCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCAt                                          <  2:2276774/150‑1 (MQ=255)

                                                                                                                 aaGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAAcc                                     <  1:2574393/150‑1 (MQ=255)

                                                                                                                   gACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCg                                   >  1:4704206/1‑150 (MQ=255)

                                                                                                                   gACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCg                                   >  1:1510515/1‑150 (MQ=255)

                                                                                                                    aCGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGa                                  <  2:8173314/150‑1 (MQ=255)

                                                                                                                     cGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGAt                                 <  1:2684609/150‑1 (MQ=255)

                                                                                                                      gCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATg                                <  1:7277128/150‑1 (MQ=255)

                                                                                                                      gCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATg                                <  1:1787066/150‑1 (MQ=255)

                                                                                                                      gCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATg                                >  2:3607347/1‑150 (MQ=255)

                                                                                                                      aagttCGAAAACATGGGCGCACAGATGCTGCGCGAAGTGGCTTCGAAGACCAACGACACTGCCGGTGACGGCACCACCACTGCGACCGTTCTCGGTCAGGCTATCGTTCAGGAAGGCGCCAAGGCTGTTGCCGCTGGCATGAACCCAATg                                >  1:1643053/4‑150 (MQ=255)

                                                                                                                      aagttCGAAAACATGGGCGCACAGATGCTGCGCGAAGTGGCTTCGAAGACCAACGACACTGCCGGTGACGGCACCACCACTGCGACCGTTCTCGGTCAGGCTATCGTTCAGGAAGGCGCCAAGGCTGTTGCCGCTGGCATGAACCCAATg                                <  2:1358880/147‑1 (MQ=255)

                                                                                                                         ttCGAAAACATGGGCGCACAGATGCTGCGCGAAGTGGCTTCGAAGACCAACGACACTGCCGGTGACGGCACCACCACTGCGACCGTTCTCGGTCAGGCTATCGTTCAGGAAGGCGCCAAGGCTGTTGCCGCTGGCATGAACCCAATGGAc                             >  1:5157835/1‑150 (MQ=255)

                                                                                                                          tCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCTTCGTTGACGat                                                                    >  1:2178127/1‑109 (MQ=255)

                                                                                                                          tCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCTTCGTTGACGat                                                                    <  2:2178127/110‑2 (MQ=255)

                                                                                                                            gAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTg                          >  2:9123339/1‑150 (MQ=255)

                                                                                                                                   tGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGcgcg                   <  2:1301564/150‑1 (MQ=255)

                                                                                                                                   tGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGcgcg                   <  1:6832878/150‑1 (MQ=255)

                                                                                                                                        gcCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATc              >  2:7263256/1‑150 (MQ=255)

                                                                                                                                        gcCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATc              <  1:4889466/150‑1 (MQ=255)

                                                                                                                                        gcCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATc              <  1:6503254/150‑1 (MQ=255)

                                                                                                                                          ccAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGa            >  2:3940565/1‑150 (MQ=255)

                                                                                                                                             gCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACaa         <  1:7628361/150‑1 (MQ=255)

                                                                                                                                              cTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAg        <  1:4230021/150‑1 (MQ=255)

                                                                                                                                              cTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAg        >  2:3865897/1‑150 (MQ=255)

                                                                                                                                                    aaGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCAct  >  2:2084233/1‑150 (MQ=255)

                                                                                                                                                    aaGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCAct  >  2:106540/1‑150 (MQ=255)

CGCGGTAAAAGCGACCCTGGGCCCGAAAGGCCGTAACGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACT  >  NC_002947/1549717‑1550014

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`