Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 96,132 C→G 6.4% G185R (GGC→CGC)  PP_0088 ← alkanal monooxygenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00294796,1320CG 6.4% 1002.2 / 41.1 300G185R (GGC→CGC) PP_0088alkanal monooxygenase
Reads supporting (aligned to +/- strand):  ref base C (122/158);  new base G (7/12);  total (129/171)
Fisher's exact test for biased strand distribution p-value = 6.38e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

Only 100 of 313 total aligned reads displayed.

CTCGTCGGTCTGCGCCACCACCATGGGAATGCCCAGCATCACATACGGCTTGTCCAGCGTGGTCGAGGGCTTGAAATGGTTGCGGTACACGCGAATGGCCTCGTGCATGTAGCGTGGCGCGAAATGCGAGGCGAAGGCGTAGGGCATGCCGCGCATGCCCGCCAGCTGGGCACTGAACAGGCTGGATCCCAGCAACCACATCGGCACGTCGGTGTCGTACCCCGGTACGGCGATCACTTTTTGATCATCAGTGCGTGGCCCGAGGTAGCGCGAAAGCTCCTCGACATCGTCTGGGAAGT  >  NC_002947/95983‑96281
                                                                                                                                                     |                                                                                                                                                     
 tcgtcgGTCTGCGCCACCACCATGGGAATGCCCAGCATCACATACGGCTTGTCCAGCGTGGTCGAGGGCTTGAAATGGTTGCGGTACACGCGAATGGCCTCGTGCATGTAGCGTGGCGCGAAATGCGAGGCGAAGGCGTAGGGCATGCcg                                                                                                                                                      >  2:1646797/1‑150 (MQ=255)
  cgtcgGTCTGCGCCACCACCATGGGAATGCCCAGCATCACATACGGCTTGTCCAGCGTGGTCGAGGGCTTGAAATGGTTGCGGTACACGCGAATGGCCTCGTGCATGTAGCGTGGCGCGAAATGCGAGGCGAAGGCGTAGGGCATGCcgc                                                                                                                                                     <  2:5542102/150‑1 (MQ=255)
    tcgGTCTGCGCCACCACCATGGGAATGCCCAGCATCACATACGGCTTGTCCAGCGTGGTCGAGGGCTTGAAATGGTTGCGGTACACGCGAATGGCCTCGTGCATGTAGCGTGGCGCGAAATGCGAGGCGAAGGCGTAGGGCATGCCgcgc                                                                                                                                                   <  2:7376921/150‑1 (MQ=255)
     cgGTCTGCGCCACCACCATGGGAATGCCCAGCATCACATACGGCTTGTCCAGCGTGGTCGAGGGCTTGAAATGGTTGCGGTACACGCGAATGGCCTCGTGCATGTAGCGTGGCGCGAAATGCGAGGCGAAGGCGTAGGGCATGCCgcgc                                                                                                                                                   <  1:954157/149‑1 (MQ=255)
      ggTCTGCGCCACCACCATGGGAATGCCCAGCATCACATACGGCTTGTCCAGCGTGGTCGAGGGCTTGAAATGGTTGCGGTACACGCGAATGGCCTCGTGCATGTAGCGTGGCGCGAAATGCGAGGCGAAGGCGTAGGGCATGCCGCGCAt                                                                                                                                                 <  1:3559846/150‑1 (MQ=255)
        tCTGCGCCACCACCATGGGAATGCCCAGCATCACATACGGCTTGTCCAGCGTGGTCGAGGGCTTGAAATGGTTGCGGTACACGCGAATGGCCTCGTGCATGTAGCGTGGCGCGAAATGCGAGGCGAAGGCGTAGGGCATGCCGCGCATGc                                                                                                                                               >  2:90775/1‑150 (MQ=255)
        tCTGCGCCACCACCATGGGAATGCCCAGCATCACATACGGCTTGTCCAGCGTGGTCGAGGGCTTGAAATGGTTGCGGTACACGCGAATGGCCTCGTGCATGTAGCGTGGCGCGAAATGCGAGGCGAAGGCGTAGGGCATGCCGCGCATGc                                                                                                                                               >  2:5595876/1‑150 (MQ=255)
        tCTGCGCCACCACCATGGGAATGCCCAGCATCACATACGGCTTGTCCAGCGTGGTCGAGGGCTTGAAATGGTTGCGGTACACGCGAATGGCCTCGTGCATGTAGCGTGGCGCGAAATGCGAGGCGAAGGCGTAGGGCATGCCGCGCATGc                                                                                                                                               <  2:5639243/150‑1 (MQ=255)
         cTGCGCCACCACCATGGGAATGCCCAGCATCACATTCGGCTTGTCCAGCGTGGTCGAGGGCTTGAAATGGTTGCGGTACACGCGAATGGCCTCGTGCATGTAGCGTGGCGCGAACTGCGAGGCGAAGGCGTAGGGCATGCCGCGCATGcc                                                                                                                                              <  2:2671313/150‑1 (MQ=255)
           gcgcCACCACCATGGGAATGCCCAGCATCACATACGGCTTGTCCAGCGTGGTCGAGGGCTTGAAATGGTTGCGGTACACGCGAATGGCCTCGTGCATGTAGCGTGGCGCGAAATGCGAGGCGAAGGCGTAGGGCATGCGCGGCATGccct                                                                                                                                            <  2:3690584/150‑2 (MQ=255)
           gcgcCACCACCATGGGAATGCCCAGCATCACATACGGCTTGTCCAGCGTGGTCGAGGGCTTGAAATGGTTGCGGTACACGCGAATGGCCTCGTGCATGTAGCGTGGCGCGAAATGCGAGGCGAAGGCGTAGGGCATGCCGCGCATGCCCg                                                                                                                                            >  1:4732364/1‑150 (MQ=255)
           gcgcCACCACCATGGGAATGCCCAGCATCACATACGGCGTGTCCAGCGTGGTCGAGGGCTTGAAATGGTTGCGGTACACGCGAATGGCCTCGTGCATGTAGCGTGGCGCGAAATGCGAGGCGAAGGCGTAGGGCATGCGCGGCATGccct                                                                                                                                            <  2:8552065/150‑2 (MQ=255)
            cgcCACCACCATGGGAATGCCCAGCATCACATACGGCTTGTCCAGCGTGGTCGAGGGCTTGAAATGGTTGCGGTACACGCGAATGGCCTCGTGCATGTAGCGTGGCGCGAAATGCGAGGCGAAGGCGTAGGGCATGCGCGGCATGcccta                                                                                                                                           <  2:4259027/150‑3 (MQ=255)
             gcCACCACCATGGGAATGCCCAGCATCACATACGGCTTGTCCAGCGTGGTCGAGGGCTTGAAATGGTTGCGGTACACGCGAATGGCCTCGTGCATGTAGCGTGGCGCGAAATGCGAGGCGAAGGCGTAGGGCATGCCGCGCATGCCCGcc                                                                                                                                          >  2:6598414/1‑150 (MQ=255)
             gcCACCACCATGGGAATGCCCAGCATCACATACGGCTTGTCCAGCGTGGTCGAGGGCTTGAAATGGGTGCGGTACACGCGAATGGCCTCGTGCATGTAGCGTGGCGCGAAATGCGAGGCGAAGGCGTAGGGCATGCGCGGCATGccctac                                                                                                                                          <  2:6479391/150‑4 (MQ=255)
              ccaccaccaTGGGAATGCCCAGCATCACATACGGCTTGTCCAGCGTGGTCGAGGGCTTGAAATGGTTGCGGTACACGCGAATGGCCTCGTGCATGTAGCGTGGCGCGAAATGCGAGGCGAAGGCGTAGGGCATGCCGCGCATGCCCGCCa                                                                                                                                         >  2:8483946/1‑150 (MQ=255)
               caccaccaTGGGAATGCCCAGCATCACATACGGCTTGTCCAGCGTGGTCGAGGGCTTGAAATGGTTGCGGTACACGCGAATGGCCTCGTGCATGTAGCGTGGCGCGAAATGCGAGGCGAAGGCGTAGGGCATGCCGCGCATGCCCGCCAg                                                                                                                                        >  1:7088642/1‑150 (MQ=255)
                  caccaTGGGAATGCCCAGCATCACATACGGCTTGTCCAGCGTGGTCGAGGGCTTGAAATGGTTGCGGTACACGCGAATGGCCTCGTGCATGTAGCGTGGCGCGAAATGCGAGGCGAAGGCGTAGGGCATGCCGCGCATGCCCGCCAGCTg                                                                                                                                     >  2:5263795/1‑150 (MQ=255)
                  caccaTGGGAATGCCCAGCATCACATACGGCTTGTCCAGCGTGGTCGAGGGCTTGAAATGGTTGCGGTACACGCGAATGGCCTCGTGCATGTAGCGTGGCGCGAAATGCGAGGCGAAGGCGTAGGGCATGCCGCGCATGCCCGCCAGCTg                                                                                                                                     >  2:7176267/1‑150 (MQ=255)
                      aTGGGAATGCCCAGCATCACATACGGCTTGTCCAGCGTGGTCGAGGGCTTGAAATGGTTGCGGTACACGCGAATGGCCTCGTGCATGTAGCGTGGCGCGAAATGCGAGGCGAAGGCGTAGGGCATGCCGCGCATGCCCGCCAGCTGGGCa                                                                                                                                 <  1:2786550/150‑1 (MQ=255)
                        gggAATGCCCAGCATCACATACGGCTTGTCCAGCGTGGTCGAGGGCTTGAAATGGTTGCGGTACACGCGAATGGCCTCGTGCATGTAGCGTGGCGCGAAATGCGAGGCGAAGGCGTAGGGCATGCCGCGCATGCCCGCCAGCTGGGCACt                                                                                                                               <  1:1306817/150‑1 (MQ=255)
                          gAATGCCCAGCATCACATACGGCTTGTCCAGCGTGGTCGAGGGCTTGAAATGGTTGCGGTACACGCGAATGGCCTCGTGCATGTAGCGTGGCGCGAAATGCGAGGCGAAGGCGTAGGGCATGCCGCGCATGCCCGCCAGCTGGGCACTGa                                                                                                                             <  1:4459998/150‑1 (MQ=255)
                           aaTGCCCAGCATCACATACGGCTTGTCCAGCGTGGTCGAGGGCTTGAAATGGTTGCGGTACACGCGAATGGCCTCGTGCATGTAGCGTGGCGCGAAATGCGAGGCGAAGGCGTAGGGCATGCCGCGCATGCCCGCCAGCTGGGCACTGaa                                                                                                                            <  2:6269916/150‑1 (MQ=255)
                           aaTGCCCAGCATCACATACGGCTTGTCCAGCGTGGTCGAGGGCTTGAAATGGTTGCGGTACACGCGAATGGCCTCGTGCATGTAGCGTGGCGCGAAATGCGAGGCGAAGGCGTAGGGCATGCCGCGCATGCCCGCCAGCTGGGCACTGaa                                                                                                                            <  1:4225476/150‑1 (MQ=255)
                           aaTGCCCAGCATCACATACGGCTTGTCCAGCGTGGTCGAGGGCTTGAAATGGTTGCGGTACACGCGAATGGCCTCGTGCATGTAGCGTGGCGCGAAATGCGAGGCGAAGGCGTAGGGCATGCCGCGCATGCCCGCCAGCTGGGCACTGaa                                                                                                                            <  1:4725524/150‑1 (MQ=255)
                                ccAGCATCACATACGGCTTGTCCAGCGTGGTCGAGGGCTTGAAATGGTTGCGGTACACGCGAATGGCCTCGTGCATGTAGCGTGGCGCGAAATGCGAGGCGAAGGCGTAGGGCATGCCGCGCATGCCCGCCAGCTGGGCACTGAACAGGc                                                                                                                       >  1:6444978/1‑150 (MQ=255)
                                ccAGCATCACATACGGCTTGTCCAGCGTGGTCGAGGGCTTGAAATGGTTGCGGTACACGCGAATGGCCTCGTGCATGTAGCGTGGCGCGAAATGCGAGGCGAAGGCGTAGGGCATGCCGCGCATGCCCGCCAGCTGGGCACTGAACAGGc                                                                                                                       >  1:1845940/1‑150 (MQ=255)
                                ccAGCATCACATACGGCTTGTCCAGCGTGGTCGAGGGCTTGAAATGGTTGCGGTACACGCGAATGGCCTCGTGCATGTAGCGTGGCGCGAAATGCGAGGCGAAGGCGTAGGGCATGCCGCGCATGCCCGCCAGCTGGGCACTGAACAGGc                                                                                                                       >  2:521411/1‑150 (MQ=255)
                                 cAGCATCACATACGGCTTGTCCAGCGTGGTCGAGGGCTTGAAATGGTTGCGGTACACGCGAATGGCCTCGTGCATGTAGCGTGGCGCGAAATGCGAGGCGAAGGCGTAGGGCATGCCGCGCATGCCCGCCAGCTGGGCACTGAACAGGCt                                                                                                                      <  2:1874148/150‑1 (MQ=255)
                                     aTCACATACGGCTTGTCCAGCGTGGTCGAGGGCTTGAAATGGTTGCGGTACACGCGAATGGCCTCGTGCATGTAGCGTGGCGCGAAATGCGAGGCGAAGGCGTAGGGCATGCCGCGCATGCCCGCCAGCTGGGCACTGAACAGGCTGGAt                                                                                                                  >  1:1384663/1‑150 (MQ=255)
                                     aTCACATACGGCTTGTCCAGCGTGGTCGAGGGCTTGAAATGGTTGCGGTACACGCGAATGGCCTCGTGCATGTAGCGTGGCGCGAAATGCGAGGCGAAGGCGTAGGGCATGCCGCGCATGCCCGCCAGCTGGGCACTGAACAGGCTGGAt                                                                                                                  <  1:6494765/150‑1 (MQ=255)
                                      tCACATACGGCTTGTCCAGCGTGGTCGAGGGCTTGAAATGGTTGCGGGACACGCGAATGGCCTCGTGCATGTAGCGTGGCGCGAAATGCGAGGCGAAGGCGTAGGGCATGCCGCGCATGCCCGCCAGCTGGGCACTGAACAGGCTGGATc                                                                                                                 <  1:3912906/150‑1 (MQ=255)
                                       cacaTACGGCTTGTCCAGCGTGGTCGAGGGCTTGAAATGGTTGCGGTACACGCGAATGGCCTCGTGCATGTAGCGTGGCGCGAAATGCGAGGCGAAGGCGTAGGGCATGCCGCGCATGCCCGCCAGCTGGGCACTGAACAGGCTGGATcc                                                                                                                >  1:4589213/1‑150 (MQ=255)
                                         caTACGGCTTGTCCAGCGTGGTCGAGGGCTTGAAATGGTTGCGGTACACGCGCATGGCCTCGTGCATGTAGCGTGGCGCGAAATGCGAGGCGAAGGCGTAGGGCATGCCGCGCATGCCCGCCAGCTGGGCACTGAACAGGCTGGATCCCa                                                                                                              <  2:6976245/150‑1 (MQ=255)
                                         caTACGGCTTGTCCAGCGTGGTCGAGGGCTTGAAATGGTTGCGGTACACGCGAATGGCCTCGTGCATGTAGCGTGGCGCGAAATGCGAGGCGAAGGCGTAGGGCATGCCGCGCATGCCCGCCAGCTGGGCACTGAACAGGCTGGATCCCa                                                                                                              <  1:5052505/150‑1 (MQ=255)
                                         caTACGGCTTGTCCAGCGTGGTCGAGGGCTTGAAATGGTTGCGGTACACGCGAATGGCCTCGTGCATGTAGCGTGGCGCGAAATGCGAGGCGAAGGCGTAGGGCATGCCGCGCATGCCCGCCAGCTGGGCACTGAACAGGCTGGATCCCa                                                                                                              <  2:8191930/150‑1 (MQ=255)
                                               gCTTGTCCAGCGTGGTCGAGGGCTTGAAATGGTTGCGGTACACGCGAATGGCCTCGTGCATGTAGCGTGGCGCGAAATGCGAGGCGAAGGCGTAGGGCATGCCGCGCATGCCCGCCAGCTGGGCACTGAACAGGCTGGATCCCAGCAAcc                                                                                                        >  1:1212528/1‑150 (MQ=255)
                                                 ttGTCCAGCGTGGTCGAGGGCTTGAAATGGTTGCGGTACACGCGAATGGCCTCGTGCATGTAGCGTGGCGCGAAATGCGAGGCGAAGGCGTAGGGCATGCCGCGCATGCCCGCCAGCTGGGCACTGAACAGGCTGGATCCCAGCAACcac                                                                                                      >  2:1202595/1‑150 (MQ=255)
                                                   gTCCAGCGTGGTCGAGGGCTTGAAATGGTTGCGGAACACGCGAATCGCCTCGTGCATGTAGCGTGGCGCGAAATGCGAGGCGAAGGCGTAGGGCATGCGGCGCATGCCCGCCAGCTGGGCACTGAACAGGCTGGATCCCAGCAACCACAt                                                                                                    <  1:6958606/150‑1 (MQ=255)
                                                      cAGCGTGGTCGAGGGCTTGAAATGGTTGCGGTACACGCGAATGGCCTCGTGCATGTAGCGTGGCGCGAAATGCGAGGCGAAGGCGTAGGGCATGCCGCGCATGCCCGCCAGCTGGGCACTGAACAGGCTGGATCCCAGCAACCACATCgg                                                                                                 >  1:7350206/1‑150 (MQ=255)
                                                         cGTGGTCGAGGGCTTGAAATGGTTGCGGTACACGCGAATGGCCTCGTGCATGTAGCGTGGCGCGAAATGCGAGGCGAAGGCGTAGGGCATGCCGCGCATGCCCGCCAGCTGGGCACTGAACAGGCTGGATCCCAGCAACCACATCGGCAc                                                                                              <  1:6717125/150‑1 (MQ=255)
                                                                gAGGGCTTGAAATGGTTGCGGTACACGCGAATGGCCTCGTGCATGTAGCGTGGCGCGAAATGCGAGGCGAAGGCGTAGGGCATGCCGCGCATGCCCGCCAGCTGGGCACTGAACAGGCTGGATCCCAGCAACCACATCGGCACGTCGgtg                                                                                       >  2:2344098/1‑150 (MQ=255)
                                                                 aGGGCTTGAAATGGTTGCGGTACACGCGAATGGCCTCGTGCATGTAGCGTGGCGCGAAATGCGAGGCGAAGGCGTAGGGCATGCCGCGCATGCCCGCCAGCTGGGCACTGAACAGGCTGGATCCCAGCAACCACATCGGCACGTCGgtgt                                                                                      <  2:6856202/150‑1 (MQ=255)
                                                                 aGGGCTTGAAATGGTTGCGGTACACGCGAATGGCCTCGTGCATGTAGCGTGGCGCGAAATGCGAGGCGAAGGCGTAGGGCATGCCGCGCATGCCCGCCAGCTGGGCACTGAACAGGCTGGATCCCAGCAACCACATCGGCACGTCGgtgt                                                                                      <  2:3780327/150‑1 (MQ=255)
                                                                  gggCTTGAAATGGTTGCGGTACACGCGAATGGCCTCGTGCATGTAGCGTGGCGCGAAATGCGAGGCGAAGGCGTAGGGCATGCCGCGCATGCCCGCCAGCTGGGCACTGAACAGGCTGGATCCCAGCAACCACATCGGCACGTCGGTGTc                                                                                     <  2:8808191/150‑1 (MQ=255)
                                                                  gggCTTGAAATGGTTGCGGTACACGCGAATGGCCTCGTGCATGTAGCGTGGCGCGAAATGCGAGGCGAAGGCGTAGGGCATGCCGCGCATGCCCGCCAGCTGGGCACTGAACAGGCTGGATCCCAGCAACCACATCGGCACGTCGGTGTc                                                                                     <  2:4814493/150‑1 (MQ=255)
                                                                   ggCTTGAAATGGTTGCGGTACACGCGAATGGCCTCGTGCATGTAGCGTGGCGCGAAATGCGAGGCGAAGGCGTAGGGCATGCCGCGCATGCCCGCCAGCTGGGCACTGAACAGGCTGGATCCCAGCAACCACATCGGCACGTCGGTGTCg                                                                                    <  1:4973142/150‑1 (MQ=255)
                                                                    gCTTGAAATGGTTGCGGTACACGCGAATGGCCTCGTGCATGTAGCGTGGCGCGAAATGCGAGGCGAAGGCGTAGGGCATGCCGCGCATGCCCGCCAGCTGGGCACTGAACAGGCTGGATCCCAGCAACCACATCGGCACGTCGGTGTCGt                                                                                   >  2:8083138/1‑150 (MQ=255)
                                                                      ttGAAATGGTTGCGGTACACGCGAATGGCCTCGTGCATGTAGCGTGGCGCGAAATGCGAGGCGAAGGCGTAGGGCATGCCGCGCACGCCCGCCAGCTGGGCACTGAACAGGCTGGATCCCAGCAACCACATCGGCACGTCGGTGTCGTAc                                                                                 >  2:3277653/1‑150 (MQ=255)
                                                                       tGAAATGGTTGCGGTACACGCGAATGGCCTCGTGCATGTAGCGTGGCGCGAAATGCGAGGCGAAGGCGTAGGGCATGCCGCGCATGCCCGCCAGCTGGGCACTGAACAGGCTGGATCCCAGCAACCACATCGGCACGTCGGTGTCGTAcc                                                                                <  1:1453021/150‑1 (MQ=255)
                                                                           aTGGTTGCGGTACACGCGAATGGCCTCGTGCATGTAGCGTGGCGCGAAATGCGAGGCGAAGGCGTAGGGCATGCCGCGCATGCCCGCCAGCTGGGCACTGAACAGGCTGGATCCCAGCAACCACATCGGCACGTCGGTGTCGTACCCCgg                                                                            >  1:7561454/1‑150 (MQ=255)
                                                                              gTTGCGGTACACGCGAATGGCCTCGTGCATGTAGCGTGGCGCGAAATGCGAGGCGAAGGCGTAGGGCATGCCGCGCATGCCCGCCAGCTGGGCACTGAACAGGCTGGATCCCAGCAACCACATCGGCACGTCGGTGTCGTACCCCGGtcc                                                                         >  1:1056606/1‑148 (MQ=255)
                                                                                    gTACACGCGAATGGCCTCGTGCATGTAGCGTGGCGCGAAATGCGAGGCGAAGGCGTAGGGCATGCCGCGCATGCCCGCCAGCTGGGCACTGAACAGGCTGGATCCCAGCAACCACATCGGCACGTCGGTGTCGTACCCCGGTACGGCGAt                                                                   >  1:2225418/1‑150 (MQ=255)
                                                                                      acacGCGAATGGCCTCGTGCATGTAGCGTGGCGCGAAATGCGAGGCGAAGGCGTAGGGCATGCCGCGCATGCCCGCCAGCTGGGCACTGAACAGGCTGGATCCCAGCAACCACATCGGCACGTCGGTGTCGTACCCCGGTACGGCGATCa                                                                 <  1:7176267/150‑1 (MQ=255)
                                                                                        acGCGAATGGCCTCGTGCATGTAGCGTGGCGCGAAATGCGAGGCGAAGGCGTAGGGCATGCCGCGCATGCCCGCCAGCTGGGCACTGAACAGGCTGGATCCCAGCAACCACATCGGCACGTCGGTGTCGTACCCCGGTACGGCGATCACt                                                               >  1:6303684/1‑150 (MQ=255)
                                                                                        acGCGAATGGCCTCGTGCATGTAGCGTGGCGCGAAATGCGAGGCGAAGGCGTAGGGCATGCCGCGCATGCCCGCCAGCTGGGCACTGAACAGGCTGGATCCCAGCAACCACATCGGCACGTCGGTGTCGTACCCCGGTACGGCGATCACt                                                               >  2:8957055/1‑150 (MQ=255)
                                                                                          gcgAATGGCCTCGTGCATGTAGCGTGGCGCGAAATGCGAGGCGAAGGCGTAGGGCATGCCGCGCATGCCCGCCAGCTGGGCACTGAACAGGCTGGATCCCAGCAACCACATCGGCACGTCGGTGTCGTACCCCGGTACGGCGATCACttt                                                             <  2:6928778/150‑1 (MQ=255)
                                                                                            gAATGGCCTCGTGCATGTAGCGTGGCGCGAAATGCGAGGCGAAGGCGTAGGGCATGCCGCGCATGCCCGCCAGCTGGGCACTGAACAGGCTGGATCCCAGCAACCACATCGGCACGTCGGTGTCGTACCCCGGTACGGCGATCACttttt                                                           <  1:5678230/150‑1 (MQ=255)
                                                                                            gAATGGCCTCGTGCATGTAGCGTGGCGCGAAATGCGAGGCGAAGGCGTAGGGCATGCCGCGCATGCCCGCCAGCTGGGCACTGAACAGGCTGGATCCCAGCAACCACATCGGCACGTCGGTGTCGTACCCCGGTACGGCGATCACttttt                                                           >  1:5862028/1‑150 (MQ=255)
                                                                                            gAATGGCCTCGTGCATGTAGCGTGGCGCGAAATGCGAGGCGAAGGCGTAGGGCATGCCGCGCATGCCCACCAGCTGGGCACTGAACAGGCTGGATCCCAGCAACCACATCGGCACGTCGGTGTCGTACCCCGGTACGGCGATCACttttt                                                           <  1:2646896/150‑1 (MQ=255)
                                                                                              aTGGCCTCGTGCATGTAGCGTGGCGCGAAATGCGAGGCGAAGGCGTAGGGCATGCCGCGCATGCCCGCCAGCTGGGCACTGAACAGGCTGGATCCCAGCAACCACATCGGCACGTCGGTGTCGTACCCCGGTACGGCGATCACTTTTTGa                                                         <  2:2827263/150‑1 (MQ=255)
                                                                                                 gCCTCGTGCATGTAGCGTGGCGCGAAATGCGAGGCGAAGGCGTAGGGCATGCCGCGCATGCCCGCCAGCTGGGCACTGAACAGGCTGGATCCCAGCAACCACATCGGCACGTCGGTGTCGTACCCCGGTACGGCGATCACTTTTTGatca                                                      <  1:214324/150‑1 (MQ=255)
                                                                                                    tCGTGCATGTAGCGTGGCGCGAAATGCGAGGCGAAGGCGTAGGGCATGCCGCGCATGCCCGCCAGCTGGGCACTGAACAGGCTGGATCCCAGCAACCACATCGGCACGTCGGTGTCGTACCCCGGTACGGCGATCACTTTTTGAtcatca                                                   >  1:7991490/1‑150 (MQ=255)
                                                                                                      gTGCATGTAGCGTGGCGCGAAATGCGCGGCGAAGGCGTAGGGCCTGCCGCGCATGCCCGCCAGCTGGGCACTGACCAGGCTGGATCCCAGCAACCACCTCGGCACGTCGGTGTCGTACCCCGGTACGGCGATCACTTTTTGATCATCAGt                                                 >  2:7603684/1‑150 (MQ=255)
                                                                                                        gCATGTAGCGTGGCGCGAAATGCGAGGCGAAGGCGTAGGGCATGCCGCGCATGCCCGCCAGCTGGGCACTGAACAGGCTGGATCCCAGCAACCACATCGGCACGTCGGTGTCGTACCCCGGTACGGCGATCACTTTTTGATCATCAGTGc                                               >  1:3646071/1‑150 (MQ=255)
                                                                                                        gCATGTAGCGTGGCGCGAAATGCGAGGCGAAGGCGTAGGGCATGCCGCGCATGCCCGCCAGCTGGGCACTGAACAGGCTGGATCCCAGCAACCACATCGGCACGTCGGTGTCGTACCCCGGTACGGCGATCACTTTTTGATCATCAGTGc                                               >  1:8765528/1‑150 (MQ=255)
                                                                                                             tAGCGTGGCGCGAAATGCGAGGCGAAGGCGTAGGGCATGCCGCGCATGCCCGCCAGCTGGGCACTGAACAGGCTGGATCCCAGCAACCACATCGGCACGTCGGTGTCGTACCCCGGTACGGCGATCACTTTTTGATCATCAGTGCGTGGc                                          <  1:8327399/150‑1 (MQ=255)
                                                                                                              aGCGTGGCGCGAAATGCGAGGCGAAGGCGTAGGGCATGCCGCGCATGCCCGCCAGCTGGGCACTGAACAGGCTGGATCCCAGCAACCACATCGGCACGTCGGTGTCGTACCCCGGTACGGCGATCACTTTTTGATCATCAGTGCGTGGcc                                         <  1:5207254/150‑1 (MQ=255)
                                                                                                               gCGTGGCGCGAAATGCGAGGCGAAGGCGTAGGGCATGCCGCGCATGCCCGCCAGCTGGGCACTGAACAGGCTGGATCCCAGCAACCACATCGGCACGTCGGTGTCGTACCCCGGTACGGCGATCACTTTTTGATCATCAGTGCGTGGccc                                        >  1:3518349/1‑150 (MQ=255)
                                                                                                                cGTGGCGCGAAATGCGAGGCGAAGGCGTAGGGCATGCCGCGCATGCCCGCCAGCTGGGCACTGAACAGGCTGGATCCCAGCAACCACATCGGCACGTCGGTGTCGTACCCCGGTACGGCGATCACTTTTTGATCATCAGTGCGTGGCCCg                                       <  1:7253439/150‑1 (MQ=255)
                                                                                                                 gTGGCGCGAAATGCGAGGCGAAGGCGTAGGGCATGTCGCGCATGCCCGCCAGCTGGGCACTGAACAGGCTGGATCCCAGCAACCACATCGGCACGTCGGTGTCGTACCCCGGTACGGCGATCACTTTTTGATCATCAGTGCGTGGCCCGa                                      <  1:606875/150‑1 (MQ=255)
                                                                                                                 gTGGCGCGAAATGCGAGGCGAAGGCGTAGGGCATGCCGCGCATGCCCGCCAGCTGGGCACTGAACAGGCTGGATCCCAGCAACCACATCGGCACGTCGGTGTCGTACCCCGGTACGGCGATCACTTTTTGATCATCAGTGCGTGGCCCGa                                      <  2:7139537/150‑1 (MQ=255)
                                                                                                                 gTGGCGCGAAATGCGAGGCGAAGGCGTAGGGCATGCCGCGCATGCCCGCCAGCTGGGCACTGAACAGGCTGGATCCCAGCAACCACATCGGCACGTCGGTGTCGTACCCCGGTACGGCGATCACTTTTTGATCATCAGTGCGTGGCCCGa                                      <  1:7831613/150‑1 (MQ=255)
                                                                                                                  tGGCGCGAAATGCGAGGCGAAGGCGTAGGGCATGCCGCGCATGCCCGCCAGCTGGGCACTGAACAGGCTGGATCCCAGCAACCACATCGGCACGTCGGTGTCGTACCCCGGTACGGCGATCACTTTTTGATCATCAGTGCGTGGCCCGAg                                     <  2:6119829/150‑1 (MQ=255)
                                                                                                                      gcgAAATGCGAGGCGAAGGCGTAGGGCATGCCGCGCATGCCCGCCAGCTGGGCACTGAACAGGCTGGATCCCAGCAACCACATCGGCACGTCGGTGTCGTACCCCGGTACGGCGATCACTTTTTGATCATCAGTGCGTGGCCCGAGGTAg                                 >  2:7808964/1‑150 (MQ=255)
                                                                                                                       cgAAATGCGAGGCGAAGGCGTAGGGCATGCCGCGCATGCCCGCCAGCTGGGCACTGAACAGGCTGGATCCCAGCAACCACATCGGCACGTCGGTGTCGTACCCCGGTACGGCGATCACTTTTTGATCATCAGTGCGTGGCCCGAGGTAgc                                <  1:904683/150‑1 (MQ=255)
                                                                                                                        gAAATGCGAGGCGAAGGCGTAGGGCATGCCGCGCATGCCCGCCAGCTGGGCACTGAACAGGCTGGATCCCAGCAACCACATCGGCACGTCGGTGTCGTACCCCGGTACGGCGATCACTTTTTGATCATCAGTGCGTGGCCCGAGGTAgcg                               >  1:5567255/1‑150 (MQ=255)
                                                                                                                        gAAATGCGAGGCGAAGGCGTAGGGCATGCCGCGCATGCCCGCCAGCTGGGCACTGAACAGGCTGGATCCCAGCAACCACATCGGCACGTCGGTGTCGTACCCCGGTACGGCGATCACTTTTTGATCATCAGTGCGTGGCCCGAGGTAgcg                               >  1:2036930/1‑150 (MQ=255)
                                                                                                                            tGCGAGGCGAAGGCGTAGGGCATGCCGCGCATGCCCGCCAGCTGGGCACTGAACAGGCTGGATCCCAGCAACCACATCGGCACGTCGGTGTCGTACCCCGGTACGGCGATCACTTTTTGATCATCAGTGCGTGGCCCGAGGTAGCGCGaa                           <  1:3820606/150‑1 (MQ=255)
                                                                                                                               gAGGCGAAGGCGTAGGGCATGCCGCGCATGCCCGCCAGCTGGGCACTGAACAGGCTGGATCCCAGCAACCACATCGGCACGTCGGTGTCGTACCCCGGTACGGCGATCACTTTTTGATCATCAGTGCGTGGCCCGAGGTAGCGCGAAAGc                        <  2:2033349/150‑1 (MQ=255)
                                                                                                                               gAGGCGAAGGCGTAGGGCATGCCGCGCATGCCCGCCAGCTGGGCACTGAACAGGCTGGATCCCAGCAACCACATCGGCACGTCGGTGTCGTACCCCGGTACGGCGATCACTTTTTGATCATCAGTGCGTGGCCCGAGGTAGCGCGAAAGc                        <  1:1366388/150‑1 (MQ=255)
                                                                                                                                aGGCGAAGGCGTAGGGCATGCCGCGCATGCCCGCCAGCTGGGCACTGAACAGGCTGGATCCCAGCAACCACATCGGCACGTCGGTGTCGTACCCCGGTACGGCGATCACTTTTTGATCATCAGTGCGTGGCCCGAGGTAGCGCGAAAGct                       >  1:3080482/1‑150 (MQ=255)
                                                                                                                                aGGCGAAGGCGTAGGGCATGCCGCGCATGCCCGCCAGCTGGGCACTGAACAGGCTGGATCCCAGCAACCACATCGGCACGTCGGTGTCGTACCCCGGTACGGCGATCACTTTTTGATCATCAGTGCGTGGCCCGAGGTAGCGCGAAAGct                       >  1:1945974/1‑150 (MQ=255)
                                                                                                                                  gCGAAGGCGTAGGGCATGCCGCGCATGCCCGCCAGCTGGGCACTGAACAGGCTGGATCCCAGCAACCACATCGGCACGTCGGTGTCGTACCCCGGTACGGCGATCACTTTTTGATCATCAGTGCGt                                             >  1:8930514/1‑126 (MQ=255)
                                                                                                                                  gCGAAGGCGTAGGGCATGCCGCGCATGCCCGCCAGCTGGGCACTGAACAGGCTGGATCCCAGCAACCACATCGGCACGTCGGTGTCGTACCCCGGTACGGCGATCACTTTTTGATCATCAGTGCGTGGCCCGAGGTAGCGCGAAAGctcc                     >  2:1441874/1‑150 (MQ=255)
                                                                                                                                     aaGGCGTAGGGCATGCCGCGCATGCCCGCCAGCTGGGCACTGAACAGGCTGGATCCCAGCAACCACATCGGCACGTCGGTGTCGTACCCCGGTACGGCGATCACTTTTTGATCATCAGTGCGTGGCCCGAGGTAGCGCGAAAGCTCCTCg                  >  2:1235320/1‑150 (MQ=255)
                                                                                                                                       ggCGTAGGGCATGCCGCGCATGCCCGCCAGCTGGGCACTGAACAGGCTGGATCCCAGCAACCACATCGGCACGTCGGTGTCGTACCCCGGTACGGCGATCACTTTTTGATCATCAGTGCGTGGCCCGAGGTAGCGCGAAAGCTCCTCGAc                <  2:8906000/150‑1 (MQ=255)
                                                                                                                                        gCGTAGGGCATGCCGCGCATGCCCGCCAGCTGGGCACTGAACAGGCTGGATCCCAGCAACCACATCGGCACGTCGGTGTCGTACCCCGGTACGGCGATCACTTTTTGATCATCAGTGCGTGGCCCGAGGTAGCGCGAAAGCTCCTCGACa               <  1:3371464/150‑1 (MQ=255)
                                                                                                                                        ccGTAGGGCATGCCGCGCATGCCCGCCAGCTGGGCACTGAACAGGCTGGATCCCAGCAACCACATCGGCACGTCGGTGTCGTACCCCGGTACGGCGATCACTTTTTGATCATCAGTGCGTGGCCCGAGGTAGCGCGAAAGCTCCTCGACa               <  2:2088886/149‑1 (MQ=255)
                                                                                                                                          ggcgggCATGCGCGGCATGCCCTCCAGCTGGGCACTGAACAGGCTGGATCCCAGCAACCACATCGGCACGTCGGTGTCGTACCCCGGTACGGCGATCACTTTTTGATCATCAGTGCGTGGCCCGAGGTAGCGCGAAAGCTCCTCGACATc             >  2:8589749/4‑150 (MQ=255)
                                                                                                                                          gTAGGGCATGCCGCGCATGCCCGCCAGCTGGGCACTGAACAGGCTGGATCCCAGCAACCACATCGGCACGTCGGTGTCGTACCCCGGTACGGCGATCACTTTTTGATCATCAGTGCGTGGCCCGAGGTAGCGCGAAAGCTCCTCGACATc             >  2:6032638/1‑150 (MQ=255)
                                                                                                                                             gggCATGCGCGGCATGCCCTCCAGCTGGGCACTGAACAGGCTGGATCCCAGCAACCACATCGGCACGTCGGTGTCGTACCCCGGTACGGCGATCACTTTTTGATCATCAGTGCGTGGCCCGAGGTAGCGCGAAAGCTCCTCGACATCGTc          >  1:2419818/1‑150 (MQ=255)
                                                                                                                                             gggCATGCGCGGCATGCCCGCCAGCTGGGCACTGAACAGGCTGGATCCCAGCAACCACATCGGCACGTCGGTGTCGTACCCCGGTACGGCGATCACTTTTTGATCATCAGTGCGTGGCCCGAGGTAGCGCGAAAGCTCCTCGACATCGTc          >  1:2306897/1‑150 (MQ=255)
                                                                                                                                             gggCATGCCGCGCATGCCCGCCAGCTGGGCACTGAACAGGCTGGATCCCAGCAACCACATCGGCACGTCGGTGTCGTACCCCGGTACGGCGATCACTTTTTGATCATCAGTGCGTGGCCCGAGGTAGCGCGAAAGCTCCTCGACATCGTc          <  1:1646797/150‑1 (MQ=255)
                                                                                                                                               gCATGCCGCGCATGCCCGCCAGCTGGGCACTGAACAGGCTGGATCCCAGCAACCACATCGGCACGTCGGTGTCGTACCCCGGTACGGCGATCACTTTTTGATCATCAGTGCGTGGCCCGAGGTAGCGCGAAAGCTCCTCGACATCGTCTg        <  2:7968099/150‑1 (MQ=255)
                                                                                                                                                cATGCCGCGCATGCCCGCCAGCTGGGCACTGAACAGGCTGGATCCCAGCAACCACATCGGCACGTCGGTGTCGTACCCCGGTACGGCGATCACTTTTTGATCATCAGTGCGTGGCCCGAGGTAGCGCGAAAGCTCCTCGACATCGTCTgg       <  2:3021708/150‑1 (MQ=255)
                                                                                                                                                  tGCCGCGCATGCCCGCCAGCTGGGCACTGAACAGGCTGGATCCCAGCAACCACATCGGCACGTCGGTGTCGTACCCCGGTACGGCGATCACTTTTTGATCATCAGTGCGTGGCCCGAGGTAGCGCGAAAGCTCCTCGACATCGTCTGGGa     >  2:1941177/1‑150 (MQ=255)
                                                                                                                                                   gCCGCGCATGCCCGCCAGCTGGGCACTGAACAGGCTGGATCCCAGCAACCACATCGGCACGTCGGTGTCGTACCCCGGTACGGCGATCACTTTTTGATCATCAGTGCGTGGCCCGAGGTAGCGCGAAAGCTCCTCGACATCGTCTGGGaa    <  2:4615750/150‑1 (MQ=255)
                                                                                                                                                    ccGCGCATGCCCGCCAGCTGGGCACTGAACAGGCTGGATCCCAGCAACCACATCGGCACGTCGGTGTCGTACCCCGGTACGGCGATCACTTTTTGATCATCAGTGCGTGGCCCGAGGTAGCGCGAAAGCTCCTCGACATCGTCTGGGAAg   <  2:3345053/150‑1 (MQ=255)
                                                                                                                                                     cgcgCATGCCCGCCAGCTGGGCACTGAACAGGCTGGATCCCAGCAACCACATCGGCACGTCGGTGTCGTACCCCGGTACGGCGATCACTTTTTGATCATCAGTGCGTGGCCCGAGGTAGCGCGAAAGCTCCTCGACATCGTCTGGGAAgt  <  2:4732364/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CTCGTCGGTCTGCGCCACCACCATGGGAATGCCCAGCATCACATACGGCTTGTCCAGCGTGGTCGAGGGCTTGAAATGGTTGCGGTACACGCGAATGGCCTCGTGCATGTAGCGTGGCGCGAAATGCGAGGCGAAGGCGTAGGGCATGCCGCGCATGCCCGCCAGCTGGGCACTGAACAGGCTGGATCCCAGCAACCACATCGGCACGTCGGTGTCGTACCCCGGTACGGCGATCACTTTTTGATCATCAGTGCGTGGCCCGAGGTAGCGCGAAAGCTCCTCGACATCGTCTGGGAAGT  >  NC_002947/95983‑96281

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑