| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 527,921 | 0 | G | C | 38.5% | 48.6 / 26.7 | 26 | noncoding (1156/2902 nt) | PP_23SC | 23S ribosomal RNA |
| Reads supporting (aligned to +/- strand): ref base G (9/7); new base C (2/8); total (11/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.09e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CTTAGACAGCTAGGAGGTTGGCTTAGAAGCAGCCACCCTTTAAAGAAAGCGTAATAGCTCACTAGTCGAGTCGGCCTGCGCGGAAGATGTAACGGGGCTCAAACCATGCACCGAAGCTACGGGTATCA‑TCTTA‑‑CGATGATGCGGTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTGACATGAGTAACGACAATGCGAGTGAAAAACTCGCACGCCGAA > NC_002947/527799‑528056 | cTTAGACAGCTAGGAGGTTGGCTTAGAAGCAGCCACCCTTTAAAGAAAGCGTAATAGCTCACTAGTCGAGTCGGCCTGCGCGGAAGATGTAACGGGGCTCAAACCATGCACCGAAGCTACGGGTATCA‑TCTTA‑‑Cga < 2:178757/136‑1 (MQ=32) ggaggTTGGCTTAGAAGCAGCCACCCTTTAAAGAAAGCGTAATAGCTCACTAGTCGAGTCGGCCTGCGCGGAAGATGTAACGGGGCTCAAACCATGCACCGAAGCTACGGGTATCA‑TCTTA‑‑CGATGATGCGGTaga > 2:369135/1‑136 (MQ=32) ttAGAAGCAGCCACCCTTTAAAGAAAGCGTAATAGCTCACTAGTCGAGTCGGCCTGCGCGGAAGATGTAACGGGGCTCAAACCATGCACCGAAGCTACGGGTATCA‑TCTTA‑‑CGATGATGCGGTAGAGGAGCGTTCt > 1:391382/1‑136 (MQ=32) ttAGAAGCAGCCACCCTTTAAAGAAAGCGTAATAGCTCACTAGTCGAGTCGGCCTGCGCGGAAGATGTAACGGGGCTCAAACCATGCACCGAAGCTACGGGTATCA‑TCTTA‑‑CGATGATGCGGTAGAGGAGCGTTCt > 1:140940/1‑136 (MQ=32) atcatttAAAGAAAGCGTAATAGCTCACTGGTCGAGTCGGCCTGCGCGGAAGATGTAACGGGGCT‑AAACCATGCACCGAAGCTGCGGCAGCGACGCTTATGCGTTGTTG‑GGTAGGGGAGCGTTCTGTAAGCCtgtg > 1:154094/5‑136 (MQ=0) atcatttAAAGAAAGCGTAATAGCTCACTGGTCGAGTCGGCCTGCGCGGAAGATGTAACGGGGCT‑AAACCATGCACCGAAGCTGCGGCAGCGACGCTTATGCGTTGTTG‑GGTAGGGGAGCGTTCTGTAAGCCtgtg > 1:623516/5‑136 (MQ=0) gCCTGCGCGGAAGATGTAACGGGGCTCAAACCATGCACCGAAGCTACGGGTATCA‑TCTTA‑‑CGATGATGCGGTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCg > 2:701916/1‑136 (MQ=32) cTGCGCGGAAGATGTAACGGGGCTCAAACCATGCACCGAAGCTACGGGTATCA‑TCTTA‑‑CGATGATGCGGTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGaa > 2:630953/1‑136 (MQ=32) cgGAAGATGTAACGGGGCTCAAACCATGCACCGAAGCTACGGGTATCA‑TCTTA‑‑CGATGATGCGGTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTg < 1:701916/136‑1 (MQ=32) aaGATGTAACGGGGCT‑AAACCATGCACCGAAGCTGCGGCAGCGACGCTTATGCGTTGTTG‑GGTAGGGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTGAc < 2:836927/136‑1 (MQ=0) cgctGTAACGTGCGTCAATCTATGATCCGAAGCTACGGCTCGCC‑TCTTA‑‑CCTTTAGGTGGTAGAGGAGCGATCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTTTCAGAAGTGCGAATGCTGACAt < 2:391382/133‑1 (MQ=2) aGATGTAACGGGGCTCAAACCATGCACCGAAGCTACGGGTATCA‑TCTTA‑‑CGATGATGCGGTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTGACAt < 2:733738/136‑1 (MQ=32) aGATGTAACGGGGCTCAAACCATGCACCGAAGCTACGGGTATCA‑TCTTA‑‑CGATGATGCGGTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTGACAt < 2:670083/136‑1 (MQ=32) taTGTAACGGGGCTCAAACCATGCACCGAAGCTACGGGTATCA‑TCTTA‑‑CGATGATGCGGTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTGACATg < 2:377358/135‑1 (MQ=17) taTGTAACGGGGCT‑AAACCATGCACCGAAGCTGCGGCAGCGACGCTTATGCGTTGTTG‑GGTAGGGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTGACAt < 2:154094/135‑1 (MQ=0) gATGTAACGGGGCTCAAACCATGCACCGAAGCTACGGGTATCA‑TCTTA‑‑CGATGATGCGGTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTGACATg < 2:637597/136‑1 (MQ=32) gATGTAACGGGGCT‑AAACCATGCACCGAAGCTGCGGCAGCGACGCTTATGCGTTGTTG‑GGTAGGGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTGACAt < 2:661950/136‑1 (MQ=0) gATGTAACGGGGCT‑AAACCATGCACCGAAGCTGCGGCAGCGACGCTTATGCGTTGTTG‑GGTAGGGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTGACAt < 2:695176/136‑1 (MQ=0) gATGTAACGGGGCT‑AAACCATGCACCGAAGCTGCGGCAGCGACGCTTATGCGTTGTTG‑GGTAGGGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTGACAt < 2:623516/136‑1 (MQ=0) gATGTAACGGGGCT‑AAACCATGCACCGAAGCTGCGGCAGCGACGCTTATGCGTTGTTG‑GGTAGGGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTGACAt < 2:519276/136‑1 (MQ=0) caagTAACGGGGCT‑AAACCATGCACCGAAGCTGCGGCAGCGACGCTTATGCGTTGTTG‑GGTAGGGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTGACAt < 2:764791/133‑1 (MQ=0) aaaCCATGCACCGAAGCTACGGGTATCA‑TCTTA‑‑CGATGATGCGGTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTGACATGAGTAACGACAATGCg > 1:602395/1‑136 (MQ=32) aCCGAAGCTACGGGTATCA‑TCTTA‑‑CGATGATGCGGTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTGACATGAGTAACGACAATGCGAGTGaaaaa > 1:901660/1‑136 (MQ=32) aCCGAAGCTACGGGTATCA‑TCTTA‑‑CGATGATGCGGTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTGACATGAGTAACGACAATGCGAGTGaaaaa > 2:679756/1‑136 (MQ=32) ccGAAGCTACGGGTATCA‑TCTTA‑‑CGATGATGCGGTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTGACAt < 2:660150/110‑1 (MQ=32) ccGAAGCTACGGGTATCA‑TCTTA‑‑CGATGATGCGGTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTGACAt > 1:660150/1‑110 (MQ=32) gTATCA‑TCTTA‑‑CGATGATGCGGTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTGACATGAGTAACGACAATGCGAGTGAAAAACTCGCACGCCGaa < 2:788309/136‑1 (MQ=32) | CTTAGACAGCTAGGAGGTTGGCTTAGAAGCAGCCACCCTTTAAAGAAAGCGTAATAGCTCACTAGTCGAGTCGGCCTGCGCGGAAGATGTAACGGGGCTCAAACCATGCACCGAAGCTACGGGTATCA‑TCTTA‑‑CGATGATGCGGTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTGACATGAGTAACGACAATGCGAGTGAAAAACTCGCACGCCGAA > NC_002947/527799‑528056 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |