| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,990,883 | 0 | T | C | 34.4% | 25.8 / 29.0 | 32 | intergenic (+199/‑347) | kefB‑III/PP_mr64 | glutathione‑regulated potassium/H+ antiporter/C4 ncRNA |
| Reads supporting (aligned to +/- strand): ref base T (6/15); new base C (1/10); total (7/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.74e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.28e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGCCCAAAAACTATCGCCAGGCACCTGGAAGCCATCCTGCAATTTCCTGACGATCACCTCGCC > NC_002947/5990753‑5991015 | cgGGTAAACCCTCTCCTTCGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGGCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGcc < 2:556253/136‑1 (MQ=255) gggTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCa < 1:567489/136‑1 (MQ=255) tACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGa > 1:669292/1‑136 (MQ=255) gtgGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGcgcc < 1:670782‑M2/136‑5 (MQ=255) gACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGcgcgggcg < 1:191472‑M2/136‑9 (MQ=255) gACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGcgcgggcg < 1:337024‑M2/136‑9 (MQ=255) aCGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGACGCGCCGcgcgggcgg < 1:331361‑M2/136‑10 (MQ=255) aCGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGcgcgggcgg < 1:780339‑M2/136‑10 (MQ=255) aCGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGcgcgggccg < 1:173231‑M2/136‑10 (MQ=255) cgAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCCGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGcgcgggcggcgcccaatc < 2:337440‑M2/136‑19 (MQ=255) cgAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGcgcgggcggcgcccaatc < 1:756319‑M2/136‑19 (MQ=255) cccccTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCCGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCCCAATCTCac < 1:337440/136‑2 (MQ=255) cTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGCCCAAAAAca > 2:742510/1‑135 (MQ=255) tCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGCTCTCATGGGCGACCAAAAACTa > 2:565873/1‑136 (MQ=255) tCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGACTGGTGCAGGAGGGACTGGCC‑GAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGcgcgggcggcgctcgatctcccaggcgctgaaaatcctc < 2:684192‑M2/136‑40 (MQ=255) tCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGACTGGTGCAGGAGGGACTGGCC‑GAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGcgcgggcggcgcgcgatctcacaggcgctgaaaatcctc < 1:71899‑M2/136‑40 (MQ=255) cgtACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCCCCGCGCGGGCGGCGCTCGATCTCATGGGCGCCCAAAAACTATAGCCAGGCAcc > 2:197423/1‑136 (MQ=255) cccaaggCGGTCGCGCGCAAATCCCGCAGGAATAATTGGCCCGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGcgcgggcggcgctcgatctcccaggcgctgaaactcttccggcgaacacctcccag < 1:309701‑M2/134‑57 (MQ=255) cccaaggCGGTCGCGCGCAAATCCCGCAGGAATAATTGGCCCGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGcgcgggcggcgctcgatctcccaggcgctgaaactctcccggcgaacacctcccag < 2:112026‑M2/134‑57 (MQ=255) cccaaggCGGTCGCGCGCAAATCCCGCAGGAATAATTGGCCCGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGcgcgggcggcgctcgatctcccaggcgctgaaactctcccg < 2:773694‑M2/119‑42 (MQ=255) ggCGGTCGCGCGCAAATCCCGCAGGAATAATTGGCCCGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGcgcgggcggcgctcgatctcccaggcgctgaaactctcccggcgaacacctcccaggcgct > 1:88072‑M2/1‑75 (MQ=255) gTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGCCCAAAAACTATCGCCAGGCACCTGGAAGCCATCCTGc < 2:670782/136‑1 (MQ=255) cgcgcgaCTGGTGCAGGAGGGACTGGCC‑GAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGcgcgggcggcgctcgatctcacaggcgctgaaaat < 2:484939‑M2/101‑36 (MQ=255) cgcgcgaCTGGTGCAGGAGGGACTGGCC‑GAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGcgcgggcggcgctcgatctcacaggcgctgaaaat > 1:484939‑M2/1‑66 (MQ=255) gggATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGCCCAAAAACTATCGCCAGGCACCTGGAAGCCATCCTGCAATTTCCTGACGATCACCTcgc > 2:34689/1‑136 (MQ=255) gggACTGGCC‑GAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGcgcgggcggcgctcgctctaccaggcgctgaaacactaccggccaacacctaccaggcgctgaaaataccccggcgaacacctaccac < 2:47190‑M2/136‑89 (MQ=255) gggACTGGCC‑GAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGcgcgggcggcgctcgatctcccaggcgctgaaactctcccggcgaacacctcccaggcgatgaaaatcacccgccgaacaccacccag < 1:504925‑M2/136‑89 (MQ=255) gggACTGGCC‑GAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGcgcgggcggcgctcgatctcccaggcgctgaaacactcccggcgaacacctccccggcgcagaaaatcccccggcgaacacctccccg < 2:203160‑M2/136‑89 (MQ=255) gggACTGGCC‑GAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGcgcgggcggcgctcgatctcacaggcgctgaaactctcccggcgaacacctcccaggcgctgaaaatcccccggcgaacacctcccag < 1:200765‑M2/136‑89 (MQ=255) gggACTGGCC‑GAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGcgcgggcggcgctagatctcacacgcgctgaaactctcccggcgaacacctcccaggcgctgaaaataccccgccgaacaccaaccac < 2:623797‑M2/136‑89 (MQ=255) gggACTGGCC‑GAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGcccgggcggcgctcgatcacacaggcgctgaaactcacccggcgaacacctcccaggcgcggaaaatcccccggccaacacctccccc < 1:376659‑M2/136‑89 (MQ=255) gggACTGGCC‑GAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGcccgcgcggcgctcgatctcccaggccctgaaactctcccggcgaacacctcccaggcgctgaaaatcccccggcgaacacctcccac < 2:195944‑M2/136‑89 (MQ=255) aatTGGCCCGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGcgcgggcggcgctcgatctcccagg > 2:255152‑M2/2‑47 (MQ=255) aatTGGCCCGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGcgcgggcggcgctcgatctcccagg < 1:255152‑M2/71‑26 (MQ=255) | CGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGCCCAAAAACTATCGCCAGGCACCTGGAAGCCATCCTGCAATTTCCTGACGATCACCTCGCC > NC_002947/5990753‑5991015 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |