| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,568,369:1 | +T | 28.6% | coding (548/1932 nt) | pctB ← | methyl‑accepting chemotaxis protein PctB |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,568,369 | 1 | . | T | 28.6% | 17.2 / 16.1 | 14 | coding (548/1932 nt) | pctB | methyl‑accepting chemotaxis protein PctB |
| Reads supporting (aligned to +/- strand): ref base . (6/4); new base T (2/2); total (8/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.96e-01 | |||||||||||
CATCACCTGGTCTTTATTGGGGCTGACGATCACCTGGCCATTGGCGTCGGCCAGGAAGGCGTGACCGATGCCGCCGAAGTCGACCGAGTTGATGATCTCGACGAGGGTGTCGAGGGTCACGTCACCGCCGACTA‑CA‑CCGATCAGTTCGCCATTGCGCTTGCTCTTCACCGGCGTGGCGATGGTCACCATCAGGCCACCGACTGCACCCTGGTACGGGGCGGTCAGCACGGTCTGCCCGGCGTTGGCGGCGGCGCCATA > NC_002947/2568236‑2568493 | catcaCCTGGTCTTTATTGGGGCTGACGATCACCTGGCCATTGGCGTCGGCCAGGAAGGCGTGACCGATGCCGCCGAAGTCGACCGAGTTGATGATCTCGACGAGGGTGTCGAGGGTCACGTCACCGCCGACTa‑ca > 1:128649/1‑136 (MQ=255) ggggCTGACGATCACCTGGCCATTGGCGTCGGCCAGGAAGGCGTGACCGATGCCGCCGAAGTCGACCGAGTTGATGATCTCGACGAGGGTGTCGAGGGTCACGTCACCGCCGACTA‑CA‑CCGATCAGTTCGCCATTg > 2:557696/1‑136 (MQ=255) gCTGACGATCACCTGGCCATTGGCGTCGGCCAGGAAGGCGTGACCGATGCCGCCGAAGTCGACCGAGTTGATGATCTCGACGAGGGTGTCGAGGGTCACGTCACCGCCGACTA‑CA‑CCGATCAGTTCGCCATTgcgc < 1:154395/136‑1 (MQ=255) gATCACCTGGCCATTGGCGTCGGCCAGGAAGGCGTGACCGATGCCGCCGAAGTCGACCGAGTTGATGATCTCGACGAGGGTGTCGAGGGTCACGTCACCGCCGACTA‑CA‑CCGATCAGTTCGCCATTGCGCTTGctc > 2:505575/1‑136 (MQ=255) gATCACCTGGCCATTGGCATCGGCCAGGAAGGCGTGACCGATGCCGCCGAAGTCGACCGAGTTGATGATCTCGACGAGGGTGTCGAGGGTCACGTCACCGCCGACTA‑CA‑CCGATCAGTTCGCCATTGCGCTTGctc > 2:106954/1‑136 (MQ=255) ggCCATTGGCGTCGGCCAGGAAGGCGTGACCGATGCCGCCGAAGTCGACCGAGTTGATGATCTCGACGAGGGTGTCGAGGGTCACGTCACCGCC‑ATTATTATCCGATCAGTTCGCCATTGCGCTTGCTCTTCACCg > 2:89405/1‑136 (MQ=255) ggCCATTGGCGTCGGCCAGGAAGGCGTGACCGATGCCGCCGAAGTCGACCGAGTTGATGATCTCGACGAGGGTGTCGAGGGTCACGTCACCGCC‑ATTATTATCCGATCAGTTCGCCATTGCGCTTGCTCTTCACCg > 2:317091/1‑136 (MQ=255) tGCCGACGAAGTCGACCGAGTTGATGATCTCGACGAGGGTGTCGAGGGTCACGTCACCGCCGACTA‑CA‑CCGATCAGTTCGCCATTGCGCTTGCTCTTCACCGGCGTGGCGATGGTCACCATCAGGCCACCGACTGc < 1:444331/136‑1 (MQ=255) gAGTTGATGATCTCGACGAGGGTGTCGAGGGTCACGTCACCGCCGACTa‑ca > 2:119729/1‑51 (MQ=255) gAGTTGATGATCTCGACGAGGGTGTCGAGGGTCACGTCACCGCCGACTa‑ca < 1:119729/51‑1 (MQ=255) tgatCTCGACGAGGGTGTCGAGGGTCACGTCACCGCCGACTA‑CA‑CCGATCAGTTCGCCATTGCGCTTGCTCTTCACCGGCGTGGCGATGGTCACCATCAGGCCACCGACTGCACCCTGGTACGGGGCGGTCAGCAc < 2:60679/136‑1 (MQ=255) gacgaGGGTGTCGAGGGTCACGTCACCGCC‑ATTATTATCCGATCAGTTCGCCATTGCGCTTGCTCTTCACCGGCGTGGCGATGGTCACCATCAGGCCACCGACTGCACCCTGGTACGGGGCGGTCAGCACGGTCTg < 1:89405/136‑1 (MQ=255) gacgaGGGTGTCGAGGGTCACGTCACCGCC‑ATTATTATCCGATCAGTTCGCCATTGCGCTTGCTCTTCACCGGCGTGGCGATGGTCACCATCAGGCCACCGACTGCACCCTGGTACGGGGCGGTCAGCACGGTCTg < 1:317091/136‑1 (MQ=255) acgaGGGTGTCGAGGGTCACGTCACCGCCGACTA‑CA‑CCGATCAGTTCGCCATTGCGCTTGCTCTTCACCGGCGTGGCGATGGTCACCATCAGGCCACCGACTGCACCCTGGTACGGGGCGGTCAGCACGGTCTGcc > 2:564503/1‑136 (MQ=255) cacCGCCGACTA‑CA‑CCGATCAGTTCGCCATTGCGCTTGCTCTTCACCGGCGTGGCGATGGTCACCATCAGGCCACCGACTGCACCCTGGTACGGGGCGGTCAGCACGGTCTGCCCGGCGTTGGCGGCGGCGCCATa > 2:96339/1‑136 (MQ=255) ccgACTA‑CA‑CCGATCAGTTCGCCATTGCGCTTGCTCTTCACCGGCGTGGCGATGGTCACCATCAGGCCACCGACTGc > 2:480523/1‑77 (MQ=255) ccgACTA‑CA‑CCGATCAGTTCGCCATTGCGCTTGCTCTTCACCGGCGTGGCGATGGTCACCATCAGGCCACCGACTGc < 1:480523/77‑1 (MQ=255) | CATCACCTGGTCTTTATTGGGGCTGACGATCACCTGGCCATTGGCGTCGGCCAGGAAGGCGTGACCGATGCCGCCGAAGTCGACCGAGTTGATGATCTCGACGAGGGTGTCGAGGGTCACGTCACCGCCGACTA‑CA‑CCGATCAGTTCGCCATTGCGCTTGCTCTTCACCGGCGTGGCGATGGTCACCATCAGGCCACCGACTGCACCCTGGTACGGGGCGGTCAGCACGGTCTGCCCGGCGTTGGCGGCGGCGCCATA > NC_002947/2568236‑2568493 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |