| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,823,901:1 | +T | 100% | coding (1248/1293 nt) | kguT ← | 2‑ketogluconate transporter, putative |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,823,901 | 1 | . | T | 83.3% | 27.9 / 2.0 | 12 | coding (1248/1293 nt) | kguT | 2‑ketogluconate transporter, putative |
| Reads supporting (aligned to +/- strand): ref base . (1/1); new base T (4/6); total (5/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.87e-01 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CACCTCGACGCGATCTTGCAGGCGTGCCATCAAGTCGTCGGACAGGCGTTTATACAGGACGATTCGCTTCTTCATTGCAGGTTTCCTAATGGTTCATGGCCAGACGCGAGCTATCGCGCTTTGCCCGGCC‑A‑GAGGTCTGGGAAGTATTGAGCGCAGCGGTAAGCGCCACTGCAGTCAGCAGCGCACCACACATGAACAGGTAAGAGGCGCCGGGCCCGCCCGTAGCCCCATTGAGGTAGCCCACCAGCCACGAGCCG > NC_002947/3823771‑3824027 | cACCTCGACGCGATCTTGCAGGCGTGCCATCAAGTCGTCGGACAGGCGTTTATACAGGACGATTCGCTTCTTCATTGCAGGTTTCCTAATGGTTCATGGCCAGACGCGAGCTATCGCGCTTTGCCCGGCC‑A‑GAGGt > 2:404486/1‑136 (MQ=255) aGGCGTTTATACAGGACGATTCGCTTCTTCATTGCAGGTTTCCTAATGGTTCATGGCCAGACGCGAGCTATCGCGCTTTGCCCGGCC‑ATGAGGTCTGGGAAGTATTGAGCGCAGCGGTAAGCGCCACTGCAGTcag < 1:315110/136‑1 (MQ=255) tttATACAGGACGATTCGCTTCTTCATTGCAGGTTTCCTAATGGTTCATGGCCAGACGCTAGCTATCGCGCTTTGCCCGGCC‑ATGAGGTCTGGGAAGTATTGAGCGCAGCGGTAAGCGCCACTGCAGTCAGCAgcg < 2:494386/136‑1 (MQ=255) actaTTCGCTTCTTCTTTGCAGGTTTCTTAATGTTTCATGGCCAGACGCTAGATATCGCGATTTGCCCGGCC‑ATGTGGTCTGGTAAGTATTGAGCGCAGCGGTAAGCGCCACTGCAGTCAGCAGCGCACCACACAt < 1:521524/133‑1 (MQ=255) aCGATTCGCTTCTTCATTGCAGGTTTCCTAATGGTTCATGGCCAGACGCGAGCTATCGCGCTTTGCCCGGCC‑ATGAGGTCTGGGAAGTATTGAGCGCAGCGGTAAGCGCCACTGCAGTCAGCAGCGCACCACACAt < 1:238416/136‑1 (MQ=255) gATTCGCTTCTTCATTGCAGGTTTCCTAATGGTTCATGGCCAGACGCGAGCTATCGCGCTTTGCCCGGCC‑ATGAGGTCTGGGAAGTATTGAGCGCAGCGGTAAGCGCCACTGCAGTCAGCAGCGCACCACACATGa > 2:128001/1‑136 (MQ=255) gCTTCTTCATTGCAGGTTTCCTAATGGTTCATGGCCAGACGCGAGCTATCGCGCTTTGCCCGGCC‑ATGAGGTCTGGGAAGTATTGAGCGCAGCGGTAAGCGCCACTGCAGTCAGCAGCGCACCACACATGAACAgg > 2:100290/1‑136 (MQ=255) gCTTCTTCATTGCAGGTTTCCTAATGGTTCATGGCCAGACGCGAGCTATCGCGCTTTGCCCGGCC‑ATGAGGTCTGGGAAGTATTGAGCGCAGCGGTAAGCGCCACTGCAGTCAGCAGCGCACCACACATGAACAgg > 2:5903/1‑136 (MQ=255) tgttttCCTTTTGGTTCATGGCCAGACGCTAGCTAGCGCGCTTTGCCCGGCC‑ATGAGGTCTGGGAAGTATTGAGCGCAGCGGTAAGCGCCACTGCAGTCAGCAGCGCACCACACATGAACAGGTAAGAGGCGCCgg < 2:261247/133‑1 (MQ=255) aGGTTTCCTAATGGTTCATGGCCAGACGCGAGCTATCGCGCTTTGCCCGGCC‑ATGAGGTCTGGGAAGTATTGAGCGCAGCGGTAAGCGCCACTGCAGTCAGCAGCGCACCACACATGAACAGGTAAGAGGCGCCgg < 1:5903/136‑1 (MQ=255) ccTAATGGTTCAGGGCCACACGCGAGCTATCGCGCTTTGCCCGGCCTT‑GAGGTCTGGGAAGTATTGAGCGCAGCGGTAAGCGCCACTGCAGTCAGCAGCGCACCACACATGAACAGGTAAGAGGCGCCGGgcccgc < 1:39472/136‑1 (MQ=255) gCCCGGCC‑ATGAGGTCTGGGAAGTATTGAGCGCAGCGGTAAGCGCCACTGCAGTCAGCAGCGCACCACACATGAACAGGTAAGAGGCGCCGGGCCCGCCCGTAGCCCCATTGAGGTAGCCCACCAGCCACGAgccg > 2:442807/1‑136 (MQ=255) | CACCTCGACGCGATCTTGCAGGCGTGCCATCAAGTCGTCGGACAGGCGTTTATACAGGACGATTCGCTTCTTCATTGCAGGTTTCCTAATGGTTCATGGCCAGACGCGAGCTATCGCGCTTTGCCCGGCC‑A‑GAGGTCTGGGAAGTATTGAGCGCAGCGGTAAGCGCCACTGCAGTCAGCAGCGCACCACACATGAACAGGTAAGAGGCGCCGGGCCCGCCCGTAGCCCCATTGAGGTAGCCCACCAGCCACGAGCCG > NC_002947/3823771‑3824027 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |