Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 527,927 T→G 14.7% noncoding (1162/2902 nt) PP_23SC → 23S ribosomal RNA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947527,9270TG14.7% 93.2 / 13.8 41noncoding (1162/2902 nt)PP_23SC23S ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base T (13/22);  new base G (2/4);  total (15/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.28e-01

GGAGGTTGGCTTAGAAGCAGCCACCCTTTAAAGAAAGCGTAATAGCTCACTAGTCGAGTCGGCCTGCGCGGAAGATGTAACGGGGCTCAAACCATGCACCGAAGCTACGGGTATCA‑TCTTA‑‑CGATGATGCGGTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTGACATGAGTAACGACAATGCGAGTGAAAAACTCGCACGCCGAA  >  NC_002947/527811‑528056
                                                                                                                     |                                                                                                                                   
ggatgTTGGCTTAGAAGCAGCCATCATTTAAAGAAAGCGTAATAGCTCACTGGTCGAGTCGGCCTGCGCGGAAGATGTAACGGGGCTCAAACCATGCACCGAAGCTACGGGTATCA‑TCTTA‑‑CGATGATGCGGTaga                                                                                                                >  1:327740/1‑136 (MQ=255)
ggatgTTGGCTTAGAAGCAGCCACCCTTTAAAGAAAGCGTAATAGCTCACTAGTCGAGTCGGCCTGCGCGGAAGATGTAACGGGGCTCAAACCATGCACCGAAGCTACGGGTATCA‑TCTTA‑‑CGATGATGCGGTaga                                                                                                                >  1:369383/1‑136 (MQ=17)
ggaggTTGGCTTAGAAGCAGCCACCCTTTAAAGAAAGCGTAATAGCTCACTAGTCGAGTCGGCCTGCGCGGAAGATGTAACGGGGCTCAAACCATGCACCGAAGCTACGGGTATCA‑TCTTA‑‑CGATGATGCGGTaga                                                                                                                >  2:30437/1‑136 (MQ=32)
 gaggTTGGCTTAGAAGCAGCCACCCTTTAAAGAAAGCGTAATAGCTCACTAGTCGAGTCGGCCTGCGCGGAAGATGTAACGGGGCTCAAACCATGCACCGAAGCTACGGGTATCA‑TCTTA‑‑CGATGATGCGGTagag                                                                                                               <  2:331453/136‑1 (MQ=32)
   ggTTGGCTTAGAAGCAGCCACCCTTTAAAGAAAGCGTAATAGCTCACTAGTCGAGTCGGCCTGCGCGGAAGATGTAACGGGGCTCAAACCATGCACCGAAGCTACGGGTATCA‑TCTTA‑‑CGATGATGCGGTAgagga                                                                                                             <  2:204747/136‑1 (MQ=32)
   ggTTGGCTTAGAAGCAGCCACCCTTTAAAGAAAGCGTAATAGCTCACTAGTCGAGTCGGCCTGCGCGGAAGATGTAACGGGGCTCAAACCATGCACCGAAGCTACGGGTATCA‑TCTTA‑‑CGATGATGCGGTAgagga                                                                                                             <  1:159849/136‑1 (MQ=32)
   ggTTGGCTTAGAAGCAGCCACCCTTTAAAGAAAGCGTAATAGCTCACTAGTCGAGTCGGCCTGCGCGGAAGATGTAACGGGGCTCAAACCATGCACCGAAGCTACGGGTATCA‑TCTTA‑‑CGATGATGCGGTAgagga                                                                                                             <  2:389212/136‑1 (MQ=32)
        gCTTAGAAGCAGCCACCCTTTAAAGAAAGCGTAATAGCTCACTAGTCGAGTCGGCCTGCGCGGAAGATGTAACGGGGCTCAAACCATGCACCGAAGCTACGGGTATCA‑TCTTA‑‑CGATGATGCGGTAGAGGAGCGtt                                                                                                        >  1:492982/1‑136 (MQ=32)
          ttAGAAGCAGCCATCATTTAAAGAAAGCGTAATAGCTCACTGGTCGAGTCGGCCTGCGCGGAAGATGTAACGGGGCTCAAACCATGCACCGAAGCTACGGGTATCA‑TCTTA‑‑CGATGATGCGGTAGAGGAGCGTTCt                                                                                                      >  1:554196/1‑136 (MQ=12)
                                  aagCGTAATAGCTCACTGGTCGAGTCGGCCTGCGCGGAAGATGTAACGGGGCT‑AAACCATGCACCGAAGCTGCGGCAGCGACGCTTATGCGTTGTTG‑GGTAGGGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTga                                                                               >  1:442270/1‑136 (MQ=0)
                                     cGTAATAGCTCACTGGTCGAGTCGGCCTGCGCGGAAGATGTAACGGGGCT‑AAACCATGCACCGAAGCTGCGGCAGCGACGCTTATGCGTTGTTG‑GGTAGGGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGaa                                                                            >  1:427453/1‑136 (MQ=0)
                                       tAATAGCTCACTAGTCGAGTCGGCCTGCGCGGAAGATGTAACGGGGCTCAAACCATGCACCGAAGCTACGGGTATCA‑TCTTA‑‑CGATGATGCGGTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCt                                                                         >  1:367397/1‑136 (MQ=32)
                                         aTAGCTCACTAGTCGAGTCGGCCTGCGCGGAAGATGTAACGGGGCTCAAACCATGCACCGAAGCTACGGGTATCA‑TCTTA‑‑CGATGATGCGGTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTg                                                                       <  1:30437/136‑1 (MQ=32)
                                         aTAGATCACTAGTCGAGTCGGCCTGCGCGGAAGATGTAACGGGGCTCAAACCATGCACCGAAGCTACGGGTATCA‑TCTTA‑‑CGATGATGCGGTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCttt                                                                       <  2:226648/136‑2 (MQ=17)
                                                  tAGTCGAGTCGGCCTGCGCGGAAGATGTAACGGGGCTCAAACCATGCACCGAAGCTACGGGTATCA‑TCTTA‑‑CGATGATGCGGTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTa                                                              >  1:379649/1‑136 (MQ=32)
                                                  tAGTCGAGTCGGCCTGCGCGGAAGATGTAACGGGGCTCAAACCATGCACCGAAGCTACGGGTATCA‑TCTTA‑‑CGATGATGCGGTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTa                                                              >  1:253086/1‑136 (MQ=32)
                                                  tAGTCGAGTCGGCCTGCGCGGAAGAAGTAACGGGGCTCAAACCATGCACCGAAGCTACGGGTATCA‑TCTTA‑‑CGATGATCCGGTAGAGGAGCGTTCTGAAAGCCTCTGAAGGTGAGTTGAGAAGCTTCCTGGAGGTa                                                              >  1:255302/1‑136 (MQ=11)
                                                                  cgcgGATGATGTAACGGCGCTCAAACCAAGCACCGAAGCTACGGGTATCA‑TCTTA‑‑CGATGATGCGGTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGc                                              <  2:255302/136‑1 (MQ=12)
                                                                  cgcgGAAGATGTAACGGGGCTCAAACCATGCACCGAAGCTACGGGTATCA‑TCTTA‑‑CGATGATGCGGTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGc                                              <  2:253086/136‑1 (MQ=32)
                                                                      gAAGATGTAACGGGGCTCAATCCATGCACCGAAGCTACGGGTATCA‑TCTTA‑‑CGATGATGCTGTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCTGAAGTGCGAATGCTGag                                          <  2:136246/136‑2 (MQ=12)
                                                                      gAAGATGTAACGGGGCTCAAACCATGCACCGAAGCTACGGGTATCA‑TCTTA‑‑CGATGATGCGGTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTGAc                                          <  2:18543/136‑1 (MQ=32)
                                                                      gAAGATGTAACGGGGCTCAAACCATGCACCGAAGCTACGGGTATCA‑TCTTA‑‑CGATGATGCGGTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTGAc                                          <  2:379649/136‑1 (MQ=32)
                                                                       aaGATGTAACGGGGCT‑AAACCATGCACCGAAGCTGCGGCAGCGACGCTTATGCGTTGTTG‑GGTAGGGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTGAc                                          <  2:111498/136‑1 (MQ=0)
                                                                        aGATGTAACGGGGCTCAATCCATGCACCTAAGCTACGGGTATCA‑TCTTA‑‑CGATGATGCGGTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTGACAt                                        <  2:269160/136‑1 (MQ=17)
                                                                        aGATGTAACGGGGCTCAAACCATGCACCGAAGCTACGGGTATCA‑TCTTA‑‑CGATGATGCGGTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTGACAt                                        <  2:492982/136‑1 (MQ=32)
                                                                         taTGTAACGGGGCTCAAACCATGCACCGAAGATACGTGTATCA‑TCTTA‑‑CGATGATTCTGTAGAGGATCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTTCGAATGCTGACATg                                       <  2:535134/135‑1 (MQ=11)
                                                                         gATGTAACGGGGCTCAAACCTTGCACCGAAGGTACTGGTATCA‑TCTTA‑‑CGATGATGCGGTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTGACATg                                       <  2:277657/136‑1 (MQ=12)
                                                                         gATGTAACGGGGCTCAAACCATGCACCGAAGCTACGGGTATCA‑TCTTA‑‑CGATGATGCGGTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTGACATg                                       <  2:554196/136‑1 (MQ=32)
                                                                         gATGTAACGGGGCT‑AAACCATGCACCGAAGCTGCGGCAGCGACGCTTATGCGTTGTTG‑GGTAGGGGAGCGTTCTGTAAGCCTGTGAAGGTGTACTGTGAGGTATGCTGGAGGTATCAGAAGTGCGAATGCTGACAt                                        <  2:200825/136‑1 (MQ=0)
                                                                         gATGTAACGGGGCT‑AAACCATGCACCGAAGCTGCGGCAGCGACGCTTATGCGTTGTTG‑GGTAGGGGAGCGTTCTGTAAGCCTGCGAAGGTGTGCTGTGAGGCATGCTGGAGGTATCAGAAGTGCGAATGCTGACAt                                        <  2:333287/136‑1 (MQ=0)
                                                                           tGTAACGGGGCT‑AAACCATGCACCGAAGCTGCGGCAGCGACGCTTATGCGTTGTTG‑GGTAGGGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTGACATGa                                      <  2:442270/136‑1 (MQ=0)
                                                                                  gggCTCAAACCATGCACCGAAGCTACGGGTATCA‑TCTTA‑‑CGATGATGCGGTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTGACATGAGTAACGAc                              <  2:361113/136‑1 (MQ=32)
                                                                                      tCAAACCATGCACCGAAGCTACGGGTATCA‑TCTTA‑‑CGATGATGCGGTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTGACATGAGTAACGACAATg                          >  1:392871/1‑136 (MQ=32)
                                                                                      tCAAACCATGCACCGAAGCTACGGGTATCA‑TCTTA‑‑CGATGATGCGGTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTGACATGAGTAACGACAATg                          >  2:413276/1‑136 (MQ=32)
                                                                                       cAAACCATGCACCGAAGCTACGGGTATCA‑TCTTA‑‑CGATGATGCGGTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTGACATGAGTAACGACAATGc                         >  2:445526/1‑136 (MQ=32)
                                                                                            cATGCACCGATGCTACGGTTATCA‑TATTA‑‑CGATTATGCGGTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCATAAGTGCTAATGCTGACATGAGTAACGACAATGCGAGTg                    <  2:369383/136‑1 (MQ=11)
                                                                                                  ccGAAGCTACGGGTATCA‑TCTTA‑‑CGATGATGCGGTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTGACATGAGTAACAACAATGCGAGTGAAAAAc              >  1:491401/1‑136 (MQ=17)
                                                                                                              ttATCA‑TCTTA‑‑CGATGATGCGGTATAGTAGCGTTCTGTAAGCGTGTGAAGGTGTGTTTAGAAGCTTGCTGGAGGTATCTGAAGTGCTAATGCTGAAATGAGTAACGACAATGCGAGTGAAAAACTCGCACGCCGaa  <  2:392871/135‑1 (MQ=11)
                                                                                                              ttATCA‑TCTTA‑‑CGATGATGCGGTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTGACATGAGTAACGACAATGCGAGTGAAAAACTCGCACGCCGaa  <  2:327740/135‑1 (MQ=17)
                                                                                                              ttATCA‑TCTTA‑‑CGATGATGCGGTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTGACATGAGTAACGACAATGCGAGTGAAAAACTCGCACGCCGaa  <  2:10669/135‑1 (MQ=17)
                                                                                                              gtctca‑tcTTA‑‑CGTGGATGCGGTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTGACATGAGTAACGACAATGCGAGTGAAAAACTCGCACGCCGaa  <  2:513919/133‑1 (MQ=12)
                                                                                                              gTATCA‑TCTTA‑‑CGATGATGCGGTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTGACATGAGTAACGACAATGCGAGTGAAAAACTCGCACGCCGaa  <  2:367397/136‑1 (MQ=32)
                                                                                                                     |                                                                                                                                   
GGAGGTTGGCTTAGAAGCAGCCACCCTTTAAAGAAAGCGTAATAGCTCACTAGTCGAGTCGGCCTGCGCGGAAGATGTAACGGGGCTCAAACCATGCACCGAAGCTACGGGTATCA‑TCTTA‑‑CGATGATGCGGTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTGACATGAGTAACGACAATGCGAGTGAAAAACTCGCACGCCGAA  >  NC_002947/527811‑528056

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑