Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 649,532 T→C 12.1% E50G (GAG→GGG)  accC ← acetyl‑CoA carboxylase biotin carboxylase subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947649,5320TC12.1% 83.8 / 4.8 33E50G (GAG→GGG) accCacetyl‑CoA carboxylase biotin carboxylase subunit
Reads supporting (aligned to +/- strand):  ref base T (11/18);  new base C (2/2);  total (13/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.67e-01

AGTCGGCGTTTTCCGCCAGGAAGCCGTAACCCGGGTGAATCGCAGTGGCGCCAGTCACTTCGGCGGCAGCGATGATCGCGGGGATATGCAGGTAGGAATCCTTGGACGATGCAGGGCCGATGCAGACCGACTCGTCTGCCAGGCCCAGGTGCATCAGTTCACGGTCGGCAGTGGAGTGCACGGCGACGGTCTTGATACCCAGCTCTTTGCAGGCACGCAGGATCCGCAGGGCAATTTCCCCGCGGTTGGCGATCAGGACTTTTTC  >  NC_002947/649401‑649665
                                                                                                                                   |                                                                                                                                     
aGTCGGCGTTTTCCGCCAGGAAGCCGTAACCCGGGTGAATCGCAGTGGCGCCAGTCACTTCGGCGGCAGCGATGATCGCGGGGATATGCAGGTAGGAATCCTTGGACGATGCAGGGCCGATGCAGACCGACTCGTc                                                                                                                                   <  2:498539/136‑1 (MQ=255)
 gTCGGCGTTTTCCGCCAGGAAGCCGTAACCCGGGTGAATCGCAGTGGCGCCAGTCACTTCGGCGGCAGCGATGATCGCGGGGATATGCAGGTAGGAATCCTTGGACGATGCAGGGCCGATGCAGACCGACTCGTCt                                                                                                                                  <  1:472526/136‑1 (MQ=255)
              gCCAGGAAGCCGTAACCCGGGTGAATCGCAGTGGCGCCAGTCACTTCGGCGGCAGCGATGATCGCGGGGATATGCAGGTAGGAATCCTTGGACGATGCAGGGCCGATGCAGACCGACTCGTCTGCCAGGCCCAGGt                                                                                                                     <  1:124192/136‑1 (MQ=255)
              gCCAGGAAGCCGTAACCCGGGTGAATCGCAGTGGCGCCAGTCACTTCGGCGGCAGCGATGATCGCGGGGATATGCAGGTAGGAATCCTTGGACGATGCAGGGCCGATGCAGACCGACTCGTCTGCCAGGCCCAGGt                                                                                                                     <  1:466205/136‑1 (MQ=255)
               ccAGGAAGCCGTAACGCGGGTGAATCGCAGTGGCGCCAGTCACTTCGGCGGCAGCGATGATCGCGGGGATATGCAGGTAGGAATCCTTGGACGATGCAGGGCCGATGCAGACCGACTCGTCTGCCAGGCCCAGGTg                                                                                                                    <  1:568370/136‑1 (MQ=255)
               ccAGGAAGCCGTAACCCGGGTGAATCGCAGTGGCGCCAGTCACTTCGGCGGCAGCGATGATCGCGGGGATATGCAGGTAGGAATCCTTGGACGATGCAGGGCCGATGCAGACCGACTCGTCTGCCAGGCCCAGGTg                                                                                                                    <  1:233955/136‑1 (MQ=255)
                        cGTAACCCGGGTGAATCGCAGTGGCGCCAGTCACTTCGGCGGCAGCGATGATCGCGGGGATATGCAGGTAGGAATCCTTGGACGATGCAGGGCCGATGCAGACCGACTCGTCTGCCAGGCCCAGGTGCATCAGTTc                                                                                                           <  1:281723/136‑1 (MQ=255)
                          tAACCCGGGTGAATCGCAGTGGCGCCAGTCACTTCGGCGGCAGCGATGATCGCGGGGATATGCAGGTAGGAATCCTTGGACGATGCAGGGCCGATGCAGACCGACTCGTCTGCCAGGCCCAGGTGCATCAGTTCAc                                                                                                         <  2:563196/136‑1 (MQ=255)
                          tAACCCGGGTGAATCGCAGTGGCGCCAGTCACTTCGGCGGCAGCGATGATCGCGGGGATATGCAGGTAGGAATCCTTGGACGATGCAGGGCCGATGCAGACCGACTCGTCTGCCAGGCCCAGGTGCATCAGTTCAc                                                                                                         <  1:452148/136‑1 (MQ=255)
                                         gCAGTGGCGCCAGTCACTTCGGCGGCAGCGATGATCGCGGGGATATGCAGGTAGGAATCCTTGGACGATGCAGGGCCGATGCAGACCGACTCGTCTGCCAGGCCCAGGTGCATCAGTTCACGGTCGGCAGTGGAGt                                                                                          <  2:256053/136‑1 (MQ=255)
                                          cAGTGGCGCCAGTCACTTCGGCGGCAGCGATGATCGCGGGGATATGCAGGTAGGAATCCTTGGACGATGCAGGGCCGATGCAGACCGACTCGTCTGCCAGGCCCAGGTGCATCAGTTCACGGTCGGCAGTGGAGTg                                                                                         >  1:242882/1‑136 (MQ=255)
                                           aGTGGCGCCAGTCACTTCGGCGGCAGCGATGATCGCGGGGATATGCAGGTAGGAATCCTTGGACGATGCAGGGCCGATGCAGACCGACTCGTCTGCCAGGCCCAGGTGCATCAGTTCACGGTCGGCAGTGGAGTGc                                                                                        >  2:469806/1‑136 (MQ=255)
                                              ggCGCCAGTCACTTCGGCGGCAGCGATGATCGCGGGGATATGCAGGTAGGAATCCTTGGACGATGCAGGGCCGATGCAGACCGACTCGTCTGCCAGGCCCAGGTGCATCAGTTCACGGTCGGCAGTGGAGTGCACg                                                                                     <  1:80590/136‑1 (MQ=255)
                                               gcgcCAGTCACTTCGGCGTCAGCGATGATCGCGGGGTTATGCTGGTAGTAATCCTTGGACGATTCAGGGCCGTTGCAGTCCGACTCGTCTGCCAGGCCCAGGTGCATCAGTTCACGGTCGGCAGTGGAGTGCACgg                                                                                    <  2:64858/136‑1 (MQ=255)
                                               gcgcCAGTCACTTCGGCGGCAGCGATGATCGCGGGGATATGCAGGTAGGAATCCTTGGACGATGCAGGGCCGATGCAGACCGACTCGTCTGCCAGGCCCAGGTGCATCAGTTCACGGTCGGCAGTGGAGTGCACgg                                                                                    <  2:391480/136‑1 (MQ=255)
                                               gcgcCAGTCACTTCGGCGGCAGCGATGATCGCGGGGATATGCAGGTAGGAATCCTTGGACGATGCAGGGCCGATGCAGACCGACTCGTCTGCCAGGCCCAGGTGCATCAGTTCACGGTCGGCAGTGGAGTGCACgg                                                                                    <  1:187572/136‑1 (MQ=255)
                                                       ctcTTCGGCGGCAGCGATGATCGCGGGGATATGCAGGTAGGAATCCTTGGACGATGCAGGGCCGATGCAGACCGACTCGTCTGCCAGGCCCAGGTGCATCAGTTCACGGTCGGCAGTGGAGTGCACGGCGACGGTc                                                                            <  1:342709/134‑1 (MQ=255)
                                                       cACTTCGGCGGCAGCGATGATCGCGGGGATATGCAGGTAGGAATCCTTGGACGATGCAGGGCCGATGCAGACCGACTCGTCTGCCAGACCCAGGTGCATCAGTTCACGGTCGGCAGTGGAGTGCACGGCGACGGTc                                                                            <  1:341007/136‑1 (MQ=255)
                                                               cggcAGCGATGATCGCGGGGATATGCAGGTAGGAATCCTTGGACGATGCAGGGCCGATGCAGACCGACTCGTCTGCCAGGCCCAGGTGCATCAGTTCACGGTCGGCAGTGGAGTGCACGGCGACGGTCTTGATAcc                                                                    <  1:484739/136‑1 (MQ=255)
                                                                                              ggAATCCTTGGACGATGCAGGGCCGATGCAGACCGACTCGTCTGCCAGGCCCAGGTGCATCAGTTCACGGTCGGCAGTGGAGTGCACGGCGACGGTCTTGATACCCAGCTCTTTGCAGGCACGCAGGATCCGCAgg                                     >  2:541492/1‑136 (MQ=255)
                                                                                                   ccTTGGACGATGCAGGGCCGATGCAGACCGACTCGTCTGCCAGGCCCAGGTGCATCAGTTCACGGTCGGCAGTGGAGTGCACGGCGACGGTCTTGATACCCAGCTCTTTGCAGGCACGCAGGATCCGCAGGGCAAt                                >  1:239727/1‑136 (MQ=255)
                                                                                                        gACGATGCAGGGCCGATGCAGACCGACTCGTCTGCCAGGCCCAGGTGCATCAGTTCACGGTCGGCAGTGGAGTGCACGGCGACGGTCTTGATACCCAGCTCTTTGCAGGCACGCAGGATCCGCAGGGCAATTTccc                           >  1:374115/1‑136 (MQ=255)
                                                                                                        gACGATGCAGGGCCGATGCAGACCGACTCGTCTGCCAGGCCCAGGTGCATCAGTTCACGGTCGGCAGTGGAGTGCACGGCGACGGTCTTGATACCCAGCTCTTTGCAGGCACGCAGGATCCGCAGGGCAATTTccc                           >  1:398914/1‑136 (MQ=255)
                                                                                                        gACGATGCAGGGCCGATGCAGACCGACTCGTCTGCCAGGCCCAGGTGCATCAGTTCACGGTCGGCAGTGGAGTGCACGGCGACGGTCTTGATACCCAGCTCTTTGCAGGCACGCAGGATCCGCAGGGCAATTTccc                           >  1:124638/1‑136 (MQ=255)
                                                                                                                aGGGCCGATGCAGACCGACTCGTCTGCCAGGCCCAGGTGCATCAGTTCACGGTCGGCAGTGGAGTGCACGGCGACGGTCTTGATACCCAGCTCTTTGCAGGCACGCAGGATCCGCAGGGCAATTTCCCCGCGGTTg                   >  1:504170/1‑136 (MQ=255)
                                                                                                                aGGGCCGATGCAGACCGACTCGTCTGCCAGGCCCAGGTGCATCAGTTCACGGTCGGCAGTGGAGTGCACGGCGACGGTCTTGATACCCAGCTCTTTGCAGGCACGCAGGATCCGCAGGGCAATTTCCCCGCGGTTg                   >  1:241919/1‑136 (MQ=255)
                                                                                                                aGGGCCGATGCAGACCGACTCGTCTGCCAGGCCCAGGTGCATCAGTTCACGGTCGGCAGTGGAGTGCACGGCGACGGTCTTGATACCCAGCTCTTTGCAGGCACGCAGGATCCGCAGGGCAATTTCCCCGCGGTTg                   >  2:449282/1‑136 (MQ=255)
                                                                                                                  ggCCGATGCAGACCGACTCGTCTGCCAGGCCCAGGTGCATCAGTTCACGGTCGGCAGTGGAGTGCACGGCGACGGTCTTGATACCCAGCTCTTTGCAGGCACGCAGGATCCGCAGGGCAATTTCCCCGCGGTTGGc                 <  2:507871/136‑1 (MQ=255)
                                                                                                                          cAGACCGACTCGTCTGCCAGGCCCAGGTGCATCAGTTCACGGTCGGCAGTGGAGTGCACGGCGACGGTCTTGATACCCAGCTCTTTGCAGGCACGCAGGATCCGCAGGGCAATTTCCCCGCGGTTGGCGATCAggg         >  1:455562/1‑135 (MQ=255)
                                                                                                                          cAGACCGACCCGTCTGCCAGGCCCAGGTGCATCAGTTCACGGTCGGCAGTGGAGTGCACGGCGACGGTCTTGAt                                                                       <  1:124344/74‑1 (MQ=255)
                                                                                                                          cAGACCGACCCGTCTGCCAGGCCCAGGTGCATCAGTTCACGGTCGGCAGTGGAGTGCACGGCGACGGTCTTGAt                                                                       <  1:127000/74‑1 (MQ=255)
                                                                                                                          cAGACCGACCCGTCTGCCAGGCCCAGGTGCATCAGTTCACGGTCGGCAGTGGAGTGCACGGCGACGGTCTTGAt                                                                       >  2:127000/1‑74 (MQ=255)
                                                                                                                          cAGACCGACCCGTCTGCCAGGCCCAGGTGCATCAGTTCACGGTCGGCAGTGGAGTGCACGGCGACGGTCTTGAt                                                                       >  2:124344/1‑74 (MQ=255)
                                                                                                                                 aatCGTCTGCCAGGCCAAGGTGCATCAGTTCACGGTCGGCAGTGGAGTGCACGGCGACGGTCTTGATACCCAGCTCTTTGCAGGCACGCAGGATCCGCTGGGCAATTTCCCCGCGGTTGGCGATCAGGACTTTTTc  <  1:219124/134‑1 (MQ=255)
                                                                                                                                   |                                                                                                                                     
AGTCGGCGTTTTCCGCCAGGAAGCCGTAACCCGGGTGAATCGCAGTGGCGCCAGTCACTTCGGCGGCAGCGATGATCGCGGGGATATGCAGGTAGGAATCCTTGGACGATGCAGGGCCGATGCAGACCGACTCGTCTGCCAGGCCCAGGTGCATCAGTTCACGGTCGGCAGTGGAGTGCACGGCGACGGTCTTGATACCCAGCTCTTTGCAGGCACGCAGGATCCGCAGGGCAATTTCCCCGCGGTTGGCGATCAGGACTTTTTC  >  NC_002947/649401‑649665

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑