| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 867,872 | G→A | 20.0% | E94E (GAG→GAA) | PP_0750 → | hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 867,872 | 0 | G | A | 20.0% | 39.6 / 5.0 | 20 | E94E (GAG→GAA) | PP_0750 | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (5/11); new base A (2/2); total (7/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.87e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.07e-01 | |||||||||||
TGCGCGTGGACGACTTCGAGCGCTTTGTAGGCTTCTTCGTCGAAGCTGGCCTGGACCTGGACGGCAAGGACGAGCACGGCAAGACCTTCGTCGAGCAGATTGCCGACCAGCGCAATGCCCCTGAGTACATCGAGATCATCGATAAAGCCCGTGGCTGATCTGTAGCCAGTGCCGGCCTCTTCGCGGGCGCGCCCGCTCCCACAGGAGTCGCGCCGCACCTGCGGGCATTACCGAACCTGTG > NC_002947/867748‑867988 | tGCGCGTGGACGACTTCGAGCGCTTTGTAGGCTTCTTCGTCGAAGCTGGCCTGGACCTGGACGGCAAGGACGAGCACGGCAAGACCTTCGTCGAGCAGATTGCCGACCAGCGCAATGCCCCTGAGTACATCgagtt > 1:486828/1‑134 (MQ=255) gTGGACGACTTCGAGCGCTTTGTAGGCTTCTTCGTCGAAGCTGGCCTGGACCTGGACGGCAAGGACGAGCACGGCAAGACCTTCGTCGAGCAGATTGCCGACCAGCGCAATGCCCCTGAGTACATCGAGATCATCg < 2:196202/136‑1 (MQ=255) gTGGACGACTTCGAGCGCTTTGTAGGCTTCTTCGTCGAAGCTGGCCTGGACCTGGACGGCAAGGACGAGCACGGCAAGACCTTCGTCGAGCAGATTGCCGACCAGCGCAATGCCCCTGAGTACATCGAGATCATCg > 2:203084/1‑136 (MQ=255) gAGCGCTTTGTAGGCTTCTTCGTCGAAGCTGGCCTGGACCTGGACGGCAAGGACGAGCACGGCAAGACCTTCGTCGAGCAGATTGCCGACCAGCGCAATGCCCCTGAGTACATCGAGATCATCGATAAAGCCCGTg < 1:350146/136‑1 (MQ=255) gAGCGCTTTGTAGGCTTCTTCGTCGAAGCTGGCCTGGACCTGGACGGCAAGGACGAGCACGGCAAGACCTTCGTCGAGCAGATTGCCGACCAGCGCAATGCCCCTGAGTACATCGAGATCATCGATAAAGCCCGTg < 1:37816/136‑1 (MQ=255) tcgtcgAAGCTGGCCTGGACCTGGACGGCAAGGACGAGCACGGCAAGACCTTCGTCGAGCAGATTGCCGACCAGCGCAATGCCCCTGAGTACATCGAGATCATCGATAAAGCCCGTGGCTGATCTGTAGCCAGTGc > 1:358093/1‑136 (MQ=255) tcgtcgAAGCTGGCCTGGACCTGGACGGCAAGGACGAGCACGGCAAGACCTTCGTCGAGCAGATTGCCGACCAGCGCAATGCCCCTGAGTACATCGAGATCATCGATAAAGCCCGTGGCTGATCTGTAGCCAGTGc > 2:103202/1‑136 (MQ=255) cgtcgAAGCTGGCCTGGACCTGGACGGCAAGGACGAGCACGGCAAGACCTTCGTCGAGCAGATTGCCGACCAGCGCAATGCCCCTGAGTACATCGAGATCATCGATAAAGCCCGTGGCTGATCTGTAGCCAGTGcc < 2:358093/136‑1 (MQ=255) ggACGAGCACGGCAAGACCTTCGTCGAGCAGATTGCCGACCAGCGCAATGCCCCTGAATACATCgaga < 1:126883/68‑1 (MQ=255) ggACGAGCACGGCAAGACCTTCGTCGAGCAGATTGCCGACCAGCGCAATGCCCCTGAATACATCgaga < 1:129611/68‑1 (MQ=255) ggACGAGCACGGCAAGACCTTCGTCGAGCAGATTGCCGACCAGCGCAATGCCCCTGAATACATCgaga > 2:126883/1‑68 (MQ=255) ggACGAGCACGGCAAGACCTTCGTCGAGCAGATTGCCGACCAGCGCAATGCCCCTGAATACATCgaga > 2:129611/1‑68 (MQ=255) aCGAGCACGGCAAGACCTTCGTCGAGCAGATTGCCGACCAGCGCAATGCCCCTGAGTACATCGAGATCATCGATAAAGCCCGTGGCTGATCTGTAGCCAGTGCCGGCCTCTTCGCGGGCGCGCCCGCTCCCACAgg < 2:26077/136‑1 (MQ=255) aCGGCAAGACCTTCGTCGAGCAGATTGCCGACCAGCGCAATGCCCCTGAGTACATCGAGATCATCGATAAAGCCCGTGGCTGATCTGTAGCCAGTGCCGGCCTCTTCGCGGGCGCGCCCGCTCCCACAGGAGTcgc > 2:111423/1‑136 (MQ=255) aGCAGATTGCCGACCAGCGCAATGCCCCTGAGTACATCGAGATCATCGATAAAGCCCGTGGCTGATCTGTAGCCAGTGCCGGCCTCTTCGCGGGCGCGCCCGCTCCCACAGGAGTCGCGCCGCACCTGCGGGCAtt < 1:111423/136‑1 (MQ=255) aGCAGATTGCCGACCAGCGCAATGCCCCTGAGTACATCGAGATCATCGATAAAGCCCGTGGCTGATCTGTAGCCAGTGCCGGCCTCTTCGCGGGCGCGCCCGCTCCCACAGGAGTCGCGCCGCACCTGCGGGCAtt < 2:290392/136‑1 (MQ=255) cAGATTGCCGACCAGCGCAATGCCCCTGAGTACATCGAGTTCATCTATAAAGCCCGTGGCTGATCTGTAGCCAGTGCCGGCCTCTTCGCGGGCGCGCCCGCTCCCACAGGAGTCGCGCCGCACCTGCGGGCATTAc < 2:486828/136‑1 (MQ=255) cAGATTGCCGACCAGCGCAATGCCCCTGAGTACATCGAGATCATCGATAAAGCCCGTGGCTGATCTGTAGCCAGTGCCGGCCTCTTCGCGGGCGCGCCCGCTCCCACAGGAGTCGCGCCGCACCTGCGGGCATTAc < 1:103202/136‑1 (MQ=255) ttttGCCGACCAGCGCTATGCCCCTGAGTACTTCGATATCATCGATAAAGCCCGTGGCTGATCTGTTGCCAGTGCCGGCCTCTTCGCGGGCGGGCAGGCTCCCACAGGATTCGCGCCGCACCTGCGGGAATTACCg < 1:203084/134‑1 (MQ=255) cccAGCGCAATGCCCCTGAGTACATCGAGATCATCGATAAAGCCCGTGGCTGATCTGTAGCCAGTGCCGGCCTCTTCGCGGGCGCGCCCGCTCCCACAGGAGTCGCGCCGCACCTGCGGGCATTACCGAACCtgtg < 1:164329/135‑1 (MQ=255) | TGCGCGTGGACGACTTCGAGCGCTTTGTAGGCTTCTTCGTCGAAGCTGGCCTGGACCTGGACGGCAAGGACGAGCACGGCAAGACCTTCGTCGAGCAGATTGCCGACCAGCGCAATGCCCCTGAGTACATCGAGATCATCGATAAAGCCCGTGGCTGATCTGTAGCCAGTGCCGGCCTCTTCGCGGGCGCGCCCGCTCCCACAGGAGTCGCGCCGCACCTGCGGGCATTACCGAACCTGTG > NC_002947/867748‑867988 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |