| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,142,183 | G→C | 14.9% | D84E (GAC→GAG) | arcD‑I ← | arginine/ornithine antiporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,142,183 | 0 | G | C | 14.9% | 63.6 / 7.4 | 27 | D84E (GAC→GAG) | arcD‑I | arginine/ornithine antiporter |
| Reads supporting (aligned to +/- strand): ref base G (10/13); new base C (2/2); total (12/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.32e-01 | |||||||||||
TGGCGGCTGGGGTGTTGCCTTCACCGAAGATCGGGAAGAAGTAGCCCAGGGTGCTGAACAGCAGCACGAAGTAGCCGACGTTGCCCAGCCAGGCACTGATCCAGTAGCCCCAGGCTGAGGAAAAGCCCATATAGTCGCCGAAGCCAGCCTTGGCATACGCATACACCCCACCATCCAGGTCGGGCTTGCGGTTGGCCAGGGTCTGGAACACGAAGGCCAGGGTCAGCATGCCGACTGCGGTGATCGCCCAGCC > NC_002947/1142050‑1142302 | tGGCGGCTGGGGTGTTGCCTTCACCGAAGATCGGGAAGAAGTAGCCCAGGGTGCTGAACAGCAGCACGAAGTAGCCGACGTTGCCCAGCCAGGCACTGATCCAGTAGCCCCAGGCTGAGGAAAAGCCCATATAGTc < 1:104634/136‑1 (MQ=255) gtgtTGCCTTCACCGAAGATCGGGAAGAAGTAGCCCAGGGTGCTGAACAGCAGCACGAAGTAGCCGACGTTGCCCAGCCAGGCACTGATCCAGTAGCCCCAGGCTGAGGAAAAGCCCATATAGTCGCCGAagccag < 2:334454/136‑1 (MQ=255) gtgtTGCCTTCACCGAAGATCGGGAAGAAGTAGCCCAGGGTGCTGAACAGCAGCACGAAGTAGCCGACGTTGCCCAGCCAGGCACTGATCCAGTAGCCCCAGGCTGAGGAAAAGCCCATATAGTCGCCGAagccag < 2:221112/136‑1 (MQ=255) ccTTCACCGAAGATCGGGAAGAAGTAGCCCAGGGTGCTGAACAGCAGCACGAAGTAGCCGACGTTGCCCAGCCAGGCACTGATCCAGTAGCCCCAGGCTGAGGAAAAGCCCATATAGTCGCCGAAGCCAGCCTTgg < 1:261878/136‑1 (MQ=255) agtagaaGCCCAGGGTGCTGAACAGCAGCACGAAGTAGCCGACGTTGCCCAGCCAGGCACTGATCCAGTAGCCCCAGGCTGAGGAAAAGCCCATATAGTCGCCGAAGCCAGCCTTGGCATACGCATACACCccacc < 1:568491/130‑1 (MQ=255) agaagTAGCCCAGGGTGCTGAACAGCAGCACGAAGTAGCCGACGTTGCCCAGCCAGGCACTGATCCAGTAGCCCCAGGCTGAGGAAAAGCCCATATAGTCGCCGAAGCCAGCCTTGGCATACGCATACACCccacc < 2:274198/136‑1 (MQ=255) agaaGCCCAGGGTGCTGAACAGCAGCACGAAGTAGCCGACGTTGCCCAGCCAGGCACTGATCCAGTAGCCCCAGGCTGAGGAAAAGCCCATATAGTCGCCGAAGCCAGCCTTGGCATACGCATACACCCCACCATc > 2:27225/4‑136 (MQ=255) tGCTGAACAGCAGCACGAAGTAGCCGACGTTGCCCAGCCAGGCACTGATCCAGTAGCCCCAGGCTGAGGAAAAGCCCATGTACTc > 1:226154/1‑85 (MQ=255) tGCTGAACAGCAGCACGAAGTAGCCGACGTTGCCCAGCCAGGCACTGATCCAGTAGCCCCAGGCTGAGGAAAAGCCCATGTACTc < 2:226154/85‑1 (MQ=255) tGCTGAACAGCAGCACGAAGTAGCCGACGTTGCCCAGCCAGGCACTGATCCAGTAGCCCCAGGCTGAGGAAAAGCCCATATAGTc > 2:468467/1‑85 (MQ=255) tGCTGAACAGCAGCACGAAGTAGCCGACGTTGCCCAGCCAGGCACTGATCCAGTAGCCCCAGGCTGAGGAAAAGCCCATATAGTc < 1:468467/85‑1 (MQ=255) tGCTGAACAGCAGCACGAAGTAGCCGACGTTGCCCAGCCAGGCACTGATCCAGTAGCCCCAGGCTGAGGAAAAGCCCATATAGTCGCCGAAGCCAGCCTTGGCATACGCATACACCCCACCATCCAGGTCGGGCtt > 1:422232/1‑136 (MQ=255) tGCTGAACAGCAGCACGAAGTAGCCGACGTTGCCCAGCCAGGCACTGATCCAGTAGCCCCAGGCTGAGGAAAAGCCCATATAGTCGCCGAAGCCAGCCTTGGCATACGCATACACCCCACCATCCAGGTCGGGCtt > 1:161663/1‑136 (MQ=255) gAAGTAGCCGACGTTGCCCAGCCAGGCACTGATCCAGTAGCCCCAGGCTGAGGAAAAGCCCATATAGTCGCCGAAGCCAGCCTTGGCATACGCATACACCCCACCATCCAGGTCGGGCTTGCGGTTGGCCAGGGTc < 1:27225/136‑1 (MQ=255) aGCCGACGTTGCCCAGCCAGGCACTGATCCAGTAGCCCCAGGCTGAGGAAAAGCCCATATAGTCGCCGAAGCCAGCCTTGGCATACGCATACACCCCACCATCCAGGTCGGGCTTGCGGTTGGCCAGGGTCTGGaa > 2:345281/1‑136 (MQ=255) gTTGCCCAGCCAGGCACTGATCCAGTAGCCCCAGGCTGAGGAAAAGCCCATATAGTCGCCGAAGCCAGCCTTGGTATACGCATACACCCCACCATCCAGGTCGGGCTTGCGGTTGGCCAGGGTCTGGAACACGAAg < 1:503835/136‑1 (MQ=255) ttGCCCAGCCAGGCGCTGATCCAGTAGCCCCAGGCCGAGGAAAAGCCCATGTACTCGCCAAAGCCAGCCTTGGCATACGCATACACCCCACCATGCAGGTCGGGCTTGCGGTTGGCCAGTGTCTGGAACACGAAg < 1:40236/135‑1 (MQ=14) ttGCCCAGCCAGGCGCTGATCCAGTAGCCCCAGGCCGAGGAAAAGCCCATGTACTCGCCAAAGCCAGCCTTGGCATACGCATACACCCCACCATCCAGGTCGGGCTTGCGGTTGGCCAGGGTCTGGAACACGAAg > 2:40236/1‑135 (MQ=14) ttGCCCAGCCAGGCACTGATCCAGTAGCCCCAGGCTGAGGAAAAGCCCATATAGTCGCCGAAGCCAGCCTTGgcatacgcat > 2:19983/1‑82 (MQ=255) ttGCCCAGCCAGGCACTGATCCAGTAGCCCCAGGCTGAGGAAAAGCCCATATAGTCGCCGAAGCCAGCCTTGgcatacgcat < 1:19983/82‑1 (MQ=255) ttGCCCAGCCAGGCACTGATCCAGTAGCCCCAGGCTGAGGAAAAGCCCATATAGTCGCCGAAGCCAGCCTTGGCATACGCATACACCccacc > 2:138422/1‑92 (MQ=255) ttGCCCAGCCAGGCACTGATCCAGTAGCCCCAGGCTGAGGAAAAGCCCATATAGTCGCCGAAGCCAGCCTTGGCATACGCATACACCccacc < 1:138422/92‑1 (MQ=255) cTGATCCAGTAGCCCCAGGCTGAGGAAAAGCCCATATAGTCGCCGAAGCCAGCCTAGGCATACGCATAAACAACACCATCCAGGTCGGGCTTGCGGTTGGCCAGGGACTGGAACACGAAGGCCAGGGTCAGCATGc > 1:12616/1‑136 (MQ=21) cAGTAGCCCCAGGCTGAGGAAAAGCCCATATAGTCGCCGAAGCCAGCCTTGGCATACGCATACACCCCACCATCCAGGTCGGGCTTGCGGTTGGCCAGGGTCTGGAACACGAAGGCCAGGGTCAGCATGCCGACTg > 2:467694/1‑136 (MQ=255) aGGCTGAGGAAAAGCCCATATAGTCGCCGAAGCCAGCCTTGGCATACGCATACACCCCACCATCCAGGTCGGGCTTGCGGTTGGCCAGGGTCTGGAACACGAAGGCCAGGGTCAGCATGCCGACTGCGGTGATCGc > 2:429410/1‑136 (MQ=255) cTGAGGAAAAGCCCATATAGTCGCCGAAGCCAGCCTTGGCATACGCATACACCCCACCATCCAGGTCGGGCTTGCGGTTGGCCAGGGTCTGGAACACGAAGGCCAGGGTCAGCATGCCGACTGCGGTGATCGCCCa < 2:422232/136‑1 (MQ=255) aGGAAAAGCCCATATAGTCGCCGAAGCCAGCCTTGGCATACGCATACACCCCACCATCCAGGTCGGGCTTGCGGTTGGCCAGGGTCTGGAACACGAAGGCCAGGGTCAGCATGCCGACTGCGGTGATCGCCCAGcc < 1:345281/136‑1 (MQ=255) | TGGCGGCTGGGGTGTTGCCTTCACCGAAGATCGGGAAGAAGTAGCCCAGGGTGCTGAACAGCAGCACGAAGTAGCCGACGTTGCCCAGCCAGGCACTGATCCAGTAGCCCCAGGCTGAGGAAAAGCCCATATAGTCGCCGAAGCCAGCCTTGGCATACGCATACACCCCACCATCCAGGTCGGGCTTGCGGTTGGCCAGGGTCTGGAACACGAAGGCCAGGGTCAGCATGCCGACTGCGGTGATCGCCCAGCC > NC_002947/1142050‑1142302 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |