| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,457,606 | G→A | 33.3% | R119H (CGT→CAT) | gapB → | glyceraldehyde‑3‑phosphate dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,457,606 | 0 | G | A | 33.3% | 9.6 / 6.3 | 12 | R119H (CGT→CAT) | gapB | glyceraldehyde‑3‑phosphate dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base G (3/5); new base A (2/2); total (5/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGTCCACGAGACATTCCCCCTGCTCAAGGCCATGAGCGAGCTGAAACTGGGCGCCGCATCGGTCGACCTGGGCAAGCTGGCCAACAAGTTCAAGCAGGAAGGCAATGGCCGCACTGCCGAGCAGTTCGTCCGTGAAGAGCTGGCCGACGTGGTCGGTCAGCAGAACGCTTCGGCGCGCAAAGGCACCGACGTTGTCCTGTACGGCTTCGGTCGCATCGGCCGCCTGCTGGCGCGCATCCTGATCGAGAAGACCGG > NC_002947/2457475‑2457729 | cgtccAAGAGACATTCCCCCTGCTCAAGGCCATGAGCGAGCTTAAACTGGGCTCCGCATCGGTCGACCTGGGCAAGCTGGCCAACAAGTTCAATCAGGAAGGCAATGGCCGCACTGCCGAGCAGTTCGTCCGTGaa < 2:380230/136‑1 (MQ=255) cGAGACATTCCCCCTGCTCAAGGCCATGAGCGAGCTGAAACTGGGCGCCGCATCGGTCGACCTGGGCAAGCTGGCCAACAAGTTCAAGCAGGAAGGCAATGGCCGCACTGCCGAGCAGTTCGTCCGTGAAGAGCTg > 2:272280/1‑136 (MQ=255) cAAGGCCATGAGCGAGCTGAAACTGGGCGCCGCATCGGTCGACCTGGGCAAGCTGGCCAACAAGTTCAAGCAGGAAGGCAATGGCCGCACTGCCGAGCAGTTCGTCCGTGAAGAGCTGGCCGACGTGGTCGGTcag > 1:244168/1‑136 (MQ=255) tGGGCGCCGCATCGGGCGACCTGGGCAAGCTGGCCAACAAGTTCAAGCAGGAAGGCAATGGCCGCACTGCCGAGCAGTTCGTCCGTGAAGAGCTGGCCGACGTGGTCGGTCAGCAGAACGCTTCGGCGCGCAAAgg < 1:118930/136‑1 (MQ=255) gCTGGCCAACAAGTTCAAGCAGGAAGGCAATGGCCGCACTGCCGAGCAGTTCGTCCGTGAAGAGCTGGCCGACGTGGTCGGTCAGCAGAACGCTTCGGCGCGCAAAGGCACCGACGTTGTCCTGTACGGCTTCGGt > 1:48835/1‑136 (MQ=255) cAATGGCCGCACTGCCGAGCAGTTCGTCCGTGAAGAGCTGGCCGACGTGGTCGGTCAGCAGAACGCTTCGGCGCGCAAAGGCACCGACGTTGTCCTGTACGGCTTCGGTCGCATCGGCCGCCTGCTGGCGCGCATc < 2:36035/136‑1 (MQ=255) cTGCCGAGCAGTTCGTCCGTGAAGAGCTGGCCGACGTGGTCGGTCAGCAGAACGCTTCGGCGCGCAAAGGCACCGACGTTGTCCTGTACGGCTTCGGTCGCATCGGCCGCCTGCTGGCGCGCATCCTGATCGagaa < 1:242699/136‑1 (MQ=255) aGCAGTTCGTCCGTGAAGAGCTGGCCGACGTGGTCGGTCAGCAGAACGCTTCGGCGCGCAAAGGCACCGACGTTGTCCTGTACGGCTTCGGTCGCATCGGCCGCCTGCTGGCGCGCATCCTGATCGAGAAGACCgg < 1:396317/136‑1 (MQ=255) aGTTCGTCCATGAAGAGCTGGCCGACGTGGTCGGTCAGCAGAACGCTTCGGCGCGCAAAGGCACCGACGTTGTCCTGTACGGCTTCGGTCGCATCGGCCGCCTGCTGGCGCGCATCCTGATCGagaa < 1:421461/127‑1 (MQ=255) aGTTCGTCCATGAAGAGCTGGCCGACGTGGTCGGTCAGCAGAACGCTTCGGCGCGCAAAGGCACCGACGTTGTCCTGTACGGCTTCGGTCGCATCGGCCGCCTGCTGGCGCGCATCCTGATCGagaa < 1:424112/127‑1 (MQ=255) aGTTCGTCCATGAAGAGCTGGCCGACGTGGTCGGTCAGCAGAACGCTTCGGCGCGCAAAGGCACCGACGTTGTCCTGTACGGCTTCGGTCGCATCGGCCGCCTGCTGGCGCGCATCCTGATCGagaa > 2:421461/1‑127 (MQ=255) aGTTCGTCCATGAAGAGCTGGCCGACGTGGTCGGTCAGCAGAACGCTTCGGCGCGCAAAGGCACCGACGTTGTCCTGTACGGCTTCGGTCGCATCGGCCGCCTGCTGGCGCGCATCCTGATCGagaa > 2:424112/1‑127 (MQ=255) | CGTCCACGAGACATTCCCCCTGCTCAAGGCCATGAGCGAGCTGAAACTGGGCGCCGCATCGGTCGACCTGGGCAAGCTGGCCAACAAGTTCAAGCAGGAAGGCAATGGCCGCACTGCCGAGCAGTTCGTCCGTGAAGAGCTGGCCGACGTGGTCGGTCAGCAGAACGCTTCGGCGCGCAAAGGCACCGACGTTGTCCTGTACGGCTTCGGTCGCATCGGCCGCCTGCTGGCGCGCATCCTGATCGAGAAGACCGG > NC_002947/2457475‑2457729 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |