Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,241,842 G→T 11.4% L336L (CTG→CTT)  PP_2837 → uronate transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,241,8420GT11.4% 113.3 / 4.9 35L336L (CTG→CTT) PP_2837uronate transporter
Reads supporting (aligned to +/- strand):  ref base G (20/11);  new base T (2/2);  total (22/13)
Fisher's exact test for biased strand distribution p-value = 6.18e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.71e-01

GCTGGTAAGGCGTGGCATGAGCGTGCTGGGCAGCCGCAAGTTCAGTGTCATCACTGGCCTGGTGCTGGCAGCGCTGTTCACCTTGCCACTGTCGTTCGTCACCGGCCTGACGGGGTGCATCATGCTGCTTTGCCTGGCGCTGTTCAGCATCAATATGGCCAGTGCCACCGCCTGGATGATCGTCAACACCATTGTCGACAGCCAGCGCGTGGCCTCGTTTGGCTCGATCCAGAACTTCGGCGGTTACATCGCAGGCTCGGTGGCACCGATC  >  NC_002947/3241707‑3241977
                                                                                                                                       |                                                                                                                                       
gctggTAAGGCGTGGCATGAGCGTGCTGGGCAGCCGCAAGTTCAGTGTCATCACTGGCCTGGTGCTGGCAGCGCTGTTCACCTTGCCACTGTCGTTCGTCACCGGCCTGACGGGGTGCATCATGCTGCTTTGCCTg                                                                                                                                         <  2:98676/136‑1 (MQ=255)
gctggTAAGGCGTGGCATGAGCGTGCTGGGCAGCCGCAAGTTCAGTGTCATCACTGGCCTGGTGCTGGCAGCGCTGTTCACCTTGCCACTGTCGTTCGTCACCGGCCTGACGGGGTGCATCATGCTGCTTTGCCTg                                                                                                                                         <  2:316125/136‑1 (MQ=255)
    gTAAGGCGTGGCATGAGCGTGCTGGGCAGCCGCAAGTTCAGTGTCATCACTGGCCTGGTGCTGGCAGCGCTGTTCACCTTGCCACTGTCGTTCGTCACCGGCCTGACGGGGTGCATCATGCTGCTTTGCCTGgcgc                                                                                                                                     >  2:451547/1‑136 (MQ=255)
     tAAGGCGTGGCATGAGCGTGCTGGGCAGCCGCAAGTTCAGTGTCATCACTGGCCTGGTGCTGGCAGCGCTGTTCACCTTGCCACTGTCGTTCGTCACCGGCCTGACGGGGTGCATCATGCTGCTTTGCCTGGCGCt                                                                                                                                    <  1:110072/136‑1 (MQ=255)
       aGGCGTGGCATGAGCGTGCTGGGCAGCCGCAAGTTCAGTGTCATCACTGGCCTGGTGCTGGCAGCGCTGTTCACCTTGCCACTGTCGTTCGTCACCGGCCTGACGGGGTGCATCATGCTGCTTTGCCTGGCGCTGt                                                                                                                                  >  1:437133/1‑136 (MQ=255)
          cGTGGCATGAGCGTGCTGGGCAGCCGCAAGTTCAGTGTCATCACTGGCCTGGTGCTGGCAGCGCTGTTCACCTTGCCACTGTCGTTCGTCACCGGCCTGACGGGGTGCATCATGCTGCTTTGCCTGGCGCTGTTCa                                                                                                                               <  2:423959/136‑1 (MQ=255)
          cGTGGCATGAGCGTGCTGGGCAGCCGCAAGTTCAGTGTCATCACTGGCCTGGTGCTGGCAGCGCTGTTCACCTTGCCACTGTCGTTCGTCACCGGCCTGACGGGGTGCATCATGCTGCTTTGCCTGGCGCTGTTCa                                                                                                                               <  2:22190/136‑1 (MQ=255)
            gggCATGAGCGTGCTGGGCAGCCGCAAGTTCAGTGTCATCACTGGCCTGGTGCTGGCAGCGCTGTTCACCTTGCCACTGTCGTTCGTCACCGGCCTGACGGGGTGCATCATGCTGCTTTGCCTGGCGCTGTTCAGc                                                                                                                             <  1:188828/135‑1 (MQ=255)
             ggCATGAGCGTGCTGGGCAGCCGCAAGTTCAGTGTCATCACTGGCCTGGTGCTGGCAGCGCTGTTCACCTTGCCACTGTCGTTCGTCACCGGCCTGACGGGGTGCATCATGCTGCTTTGCCTGGCGCTGTTCAGCa                                                                                                                            >  1:524569/1‑136 (MQ=255)
                      gTGCTGGGCAGCCGCAAGTTCAGTGTCATCACTGGCCTGGTGCTGGCAGCGCTGTTCACCTTGCCACTGTCGTTCGTCACCGGCCTGACGGGGTGCATCATGCTGCTTTGCCTGGCGCTGTTCAGCATCAATATgg                                                                                                                   >  1:383740/1‑136 (MQ=255)
                          tGGGCAGCCGCAAGTTCAGTGTCATCACTGGCCTGGTGCTGGCAGCGCTGTTCACCTTGCCACTGTCGTTCGTCACCGGCCTGACGGGGTGCATCATGCTGCTTTGCCTTGCGCTGTTCAGCATCAATATGGCCAg                                                                                                               >  1:191761/1‑136 (MQ=255)
                          tGGGCAGCCGCAAGTTCAGTGTCATCACTGGCCTGGTGCTGGCAGCGCTGTTCACCTTGCCACTGTCGTTCGTCACCGGCCTGACGGGGTGCATCATGCTGCTTTGCCTTGCGCTGTTCAGCATCAATATGGCCAg                                                                                                               >  1:380280/1‑136 (MQ=255)
                           gggCAGCCGCAAGTTCAGTGTCATCACTGGCCTGGTGCTGGCAGCGCTGTTCACCTTGCCACTGTCGTTCGTCACCGGCCTGACGGGGTGCATCATGCTGCTTTGCCTGGCGCTGTTCAGCATCAATATGGCCAGt                                                                                                              <  2:383740/136‑1 (MQ=255)
                                    cAAGTTCAGTGTAATCACTGGCCTGGTGCTGGCAGCGCTGTTCACCTTGCCACTGTCGTTCGTCACCGGCCTGACGGGGTGCATCATGCTGCTTTGCCTGGCGCTGTTCAGCATCAATATGGCCAGTGCCACCGcc                                                                                                     >  2:222477/1‑136 (MQ=255)
                                     aaGTTCAGTGTCATCACTGGCCTGGTGCTGGCAGCGCTGTTCACCTTGCCACTGTCGTTCGTCACCGGCCTGACGGGGTGCATCATGCTGCTTTGCCTGGCGCTGTTCAGCATCAATATGGCCAGTGCCACCGCCt                                                                                                    <  1:105115/136‑1 (MQ=255)
                                                          cTGGTGCTGGCAGCGCTGTTCACCTTGCCACTGTCGTTCGTCACCGGCCTGACGGGGTGCATCATGCTGCTTTGCCTGGCGCTGTTCAGCATCAATATGGCCAGTGCCACCGCCTGGATGATCGTCAACACCATTg                                                                               >  1:552925/1‑136 (MQ=255)
                                                           tGGTGCTGGCAGCGCTGTTCACCTTGCCACTGTCGTTCGTCACCGGCCTGACGGGGTGCATCATGCTGCTTTGCCTGGCGCTGTTCAGCATCAATATGGCCAGTGCCACCGCCTGGATGATCGTCAACACCATTGt                                                                              <  1:183972/136‑1 (MQ=255)
                                                                cTGGCAGCGCTGTTCACCTTGCCACTGTCGTTCGTCACCGGCCTGACGGGGTGCATCATGCTGCTTTGCCTGGCGCTGTTCAGCATCAATATGGCCAGTGCCACCGCCTGGATGATCGTCAACACCATTGTCGAca                                                                         >  1:504158/1‑136 (MQ=255)
                                                                          tGTTCACCTTGCCACTGTCGTTCGTCACCGGCCTGACGGGGTGCATCATGCTGCTTTGCCTGGCGCTGTTCAGCATCAATATGGCCAGTGCCACCGCCTGGATGATCGTCAACACCATTGTCGACAGCCAGCGCGt                                                               <  1:185811/136‑1 (MQ=255)
                                                                                     ccACTGTCGTTCGTCACCGGCCTGACGGGGTGCATCATGCTGCTTTGCCTGGCGCTGTTCAGCATCAATATGGCCAGTGCCACCGCCTGGATGATCGTCAACACCATTGTCGACAGCCAGCGCGTGGCCTCGTTTg                                                    <  2:279869/136‑1 (MQ=255)
                                                                                     ccACTGTCGTTCGTCACCGGCCTGACGGGGTGCATCATGCTGCTTTGCCTGGCGCTGTTCAGCATCAATATGGCCAGTGCCACCGCCTGGATGATCGTCAACACCATTGTCGACAGCCAGCGCGTGGCCTCGTTTg                                                    <  2:391266/136‑1 (MQ=255)
                                                                                     ccACTGTCGTTCGTCACCGGCCTGACGGGGTGCATCATGCTGCTTTGCCTGGCGCTGTTCAGCATCAATATGGCCAGTGCCACCGCCTGGATGATCGTCAACACCATTGTCGACAGCCAGCGCGTGGCCTCGTTTg                                                    <  1:222477/136‑1 (MQ=255)
                                                                                       aCTGTCGTTCGTCACCGGCCTGACGGGGTGCATCATGCTGCTTTGCCTGGCGCTGTTCAGCATCAATATGGCCAGTGCCACCGCCTGGATGATCGTCAACACCATTGTCGACAGCCAGCGCGTGGCCTCGTTTGGc                                                  >  2:529659/1‑136 (MQ=255)
                                                                                       aCTGTCGTTCGTCACCGGCCTGACGGGGTGCATCATGCTGCTTTGCCTGGCGCTGTTCAGCATCAATATGGCCAGTGCCACCGCCTGGATGATCGTCAACACCATTGTCGACAGCCAGCGCGTGGCCTCGTTTGGc                                                  >  2:516829/1‑136 (MQ=255)
                                                                                              ttcgtCACCGGCCTGACGGGGTGCATCATGCTGCTTTGCCTGGCGCTGTTCAGCATCAATATGGCCAGTGCCACCGCCTGGATGATCGTCAACACCATTGTCGACAGCCAGCGCGTGGCCTCGTTTGGCTCGATcc                                           >  2:7375/1‑136 (MQ=255)
                                                                                              ttcgtCACCGGCCTGACGGGGTGCATCATGCTGCTTTGCCTGGCGCTGTTCAGCATCAATATGGCCAGTGCCACCGCCTGGATGATCGTCAACACCATTGTCGACAGCCAGCGCGTGGCCTCGTTTGGCTCGATcc                                           >  2:4568/1‑136 (MQ=255)
                                                                                                                gggTGCATCATGCTGCTTTGCCTGGCGCTGTTCAGCATCAATATGGCCAGTGCCACCGCCTGGATGATCGTCAACACCATTGTCGACAGCCAGCGCGTGGCCTCGTTTGGCTCGATCCAGAACTTCGGCGGTTACa                         >  2:59862/1‑136 (MQ=255)
                                                                                                                     catcatGCTGCTTTGCCTGGCGCTGTTCAGCATCAATATGGCCAGTGCCACCGCCTGGATGATCGTCAACACCATTGTCGACAGCCAGCGCGTGGCCTCGTTTGGCTCGATCCAGAACTTCGGCGGTTACATCGCa                    >  1:226321/1‑136 (MQ=255)
                                                                                                                      atcatGCTGCTTTGCCTGGCGCTGTTCAGCATCAATATGGCCAGTGCCACCGCCTGGATGATCGTCAACACCATTGTCGACAGCCAGCGCGTGGCCTCGTTTGGCTCGATCCAGAACTTCGGCGGTTACATCGCAg                   >  1:192919/1‑136 (MQ=255)
                                                                                                                      atcatGCTGCTTTGCCTGGCGCTGTTCAGCATCAATATGGCCAGTGCCACCGCCTGGATGATCGTCAACACCATTGTCGACAGCCAGCGCGTGGCCTCGTTTGGCTCGATCCAGAACTTCGGCGGTTACATCGCAg                   >  2:555221/1‑136 (MQ=255)
                                                                                                                              gctTTGCCTTGCGCTGTTCAGCATCAATATGGCCAGTGCCACCGCCTGGATGATCGTCAACACCATTGTCGACAGCCAGCGCGTGGCCTCGTTTGGCTCGATCCAGAACTTCGGCGGTTACATCGCAGGCTCGGTg           <  2:380280/136‑1 (MQ=255)
                                                                                                                              gctTTGCCTTGCGCTGTTCAGCATCAATATGGCCAGTGCCACCGCCTGGATGATCGTCAACACCATTGTCGACAGCCAGCGCGTGGCCTCGTTTGGCTCGATCCAGAACTTCGGCGGTTACATCGCAGGCTCGGTg           <  2:191761/136‑1 (MQ=255)
                                                                                                                                tttGCCTGGCGCTGTTCAGCATCAATATGGCCAGTGCCACCGCCTGGATGATCGTCAACACCATTGTCGACAGCCAGCGCGTGGCCTCGTTTGGCTCGATCCAGAACTTCGGCGGTTACATCGCAGGCTCGGTGGc         >  2:265884/1‑136 (MQ=255)
                                                                                                                                tttGCCTGGCGCTGTTCAGCATCAATATGGCCAGTGCCACCGCCTGGATGATCGTCAACACCATTGTCGACAGCCAGCGCGTGGCCTCGTTTGGCTCGATCCAGAACTTCGGCGGTTACATCGCAGGCTCGGTGGc         >  1:363434/1‑136 (MQ=255)
                                                                                                                                  tGCCTGGCGCTGTTCAGCATCAATATGGCCAGTGCCACCGCCTGGATGATCGTCAACACCATTGTCGACAGCCAGCGCGTGGCCTCGTTTGGCTCGATCCAGAACTTCGGCGGTTACATCGCAGGCTCGGTGGCAc       >  1:479040/1‑136 (MQ=255)
                                                                                                                                    ccTGGCGCTGTTCAGCATCAATATGGCCAGTGCCACCGCCTGGATGATCGTCAACACCATTGTCGACAGCCAGCGCGTGGCCTCGTTTGGCTCGATCCAGAACTTCGGCGGTTACATCGCAGGCTCGGTGGCACCg     >  2:307991/1‑136 (MQ=255)
                                                                                                                                    ccTGGCGCTGTTCAGCATCAATATGGCCAGTGCCACCGCCTGGATGATCGTCAACACCATTGTCGACAGCCAGCGCGTGGCCTCGTTTGGCTCGATCCAGAACTTCGGCGGTTACATCGCAGGCTCGGTGGCACCg     >  2:304952/1‑136 (MQ=255)
                                                                                                                                       ggCGCTGTTCAGCATCAATATGGCCAGTGCCACCGCCTGGATGATCGTCAACACCATTGTCGACAGCCAGCGCGTGGCCTCGTTTGGCTCGATCCAGAACTTCGGCGGTTACATCGCAGGCTCGGTGGCACCGATc  <  2:226321/136‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                       
GCTGGTAAGGCGTGGCATGAGCGTGCTGGGCAGCCGCAAGTTCAGTGTCATCACTGGCCTGGTGCTGGCAGCGCTGTTCACCTTGCCACTGTCGTTCGTCACCGGCCTGACGGGGTGCATCATGCTGCTTTGCCTGGCGCTGTTCAGCATCAATATGGCCAGTGCCACCGCCTGGATGATCGTCAACACCATTGTCGACAGCCAGCGCGTGGCCTCGTTTGGCTCGATCCAGAACTTCGGCGGTTACATCGCAGGCTCGGTGGCACCGATC  >  NC_002947/3241707‑3241977

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑