| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,449,256 | T→G | 33.1% | A19A (GCT→GCG) | PP_5556 → | bacteriophage lysis protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,449,256 | 0 | T | G | 33.1% | 21.0 / 5.3 | 38 | A19A (GCT→GCG) | PP_5556 | bacteriophage lysis protein |
| Reads supporting (aligned to +/- strand): ref base T (21/3); new base G (5/7); total (28/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.86e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CAAACGTATCAATGGTGGTAGCAACGGTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCG‑CCAGCGCTGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTGGAGCGGG > NC_002947/3449125‑3449377 | cAAACGTATCAATGGTGGTAGCAACGGTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCG‑CCCGCGCTggcg > 2:525635/1‑136 (MQ=255) aTGGTGGTAGCAACGGTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCG‑CCACCGCTGGCGGCGCCCGGGcc > 1:454836/1‑135 (MQ=255) gCAACGGTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCG‑CCAGCGCTGGCGGCGCCCGGGCAGCCTGGgtg > 1:495856/1‑136 (MQ=255) gTCTGGAGGACCCCCAGGCCCTCTACCCGCGCGCGCTCGCGGTGCTGCAGGGCGCGCTTGGCCCCCCCGCGGGGGCGCGGGGGCGCTGCGGCCGGCCG‑CCAGCGCTGGCGGCGCCCGGGCAGCCTGGGTGTGGCAg < 2:495856/136‑1 (MQ=255) aggaTCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCG‑CCACCGCACGCGGCGCCCCGGCACCCTGGGTGTGGCAGGCCacc > 1:65577/1‑134 (MQ=255) cAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCG‑CCCGCGCGGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCttaa > 2:195787/1‑133 (MQ=255) aaGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCG‑CCCGCGCTGGCGGCGCCCGGGCAGCCGGGGTGTGGGAGGCCAACGCCTATaa > 1:201997/1‑136 (MQ=255) aaGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCG‑CCAGCGCTGGCGGCGCCCGGGCCGCCGGGGTGTGGCAGGCCCACGCCGATaa > 1:363632/1‑136 (MQ=255) taacacGCGTGCGCTCCTGGTGCTGCTGTGCGCGCTGGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCG‑CCAGCGCGGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGc < 2:221670/134‑1 (MQ=255) agcgCTCGAGGTGCTGCAGTGAGCGCTCGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCG‑CCCGCGCTGGCGGCGCCCGGGCCGCCTGGGTGTGGGAGGCCCACGCCGATAAGGCGCAGCTCGCGGATc > 2:39130/1‑136 (MQ=255) gcTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCG‑CCCGCGCTGGCGGCGCCCGGGCCGCCGGGGTGTGGCAGGCCAACGCCGATAAGGCGCCGCTCGCTGATCAgg > 1:128546/1‑136 (MQ=255) gcTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCG‑CCCGCGCTGGCGGCGCCCGGGCAGCCGGGGTGTGGCAGGCCCACGCCGATAAGGCGCCGCTCGCTGATCAgg > 1:131247/1‑136 (MQ=255) gcTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCG‑CCAGCGCTGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAgg > 2:143007/1‑136 (MQ=255) gcTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCG‑CCACCGCTGGCGGCGCCCGGGCCGCCGGGGTGGGGCAGGCCAACGCCAAAAAGGCGCAGCTCGCTGATCAgg > 1:221670/1‑136 (MQ=255) tCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCG‑CCCGCGCTGGCGGCGCCCGGGCCGCCTGGGTGTGGCCGGCCAACGCCTATAAGGCGCAGCTCGCTGCTCAGGcc > 1:525239/1‑136 (MQ=255) tCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCG‑CCCGCGCTGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCCACGCCAATAAGGCGCAGCTCGCTGATCAGGcc > 1:104695/1‑136 (MQ=255) tCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCG‑CCCGCGCGGGCGGCGCCCGGGCAGCCGGGGTGTGGCGGGCCAACGCCGATAAGCCGCCGCGCGCTGATCAGGcc > 2:472699/1‑136 (MQ=255) tCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCG‑CCCGCGCCGGCGGCGCCCGGGCCGCCGGGGTGTGGCAGGCCCCCGCCTCAAAGGAGCACCACGCGGATCAGGcc > 1:452183/1‑136 (MQ=255) tCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCG‑CCAGCGCGGGCGGCGCCCCGGCCGCCGGGGGGTGGGAGGCCAACGCCGAAAAGGCGCCGCGCGCAGATCACGcc > 1:476209/1‑136 (MQ=255) gTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCG‑CCCGCGCGGGCGGCGCCCCGGCCGCCGGGGTGTGGCCGGCCCACGCCGATAAGGCGCCGCGCGCGGATCAGGCCGCGGACTGCgggc > 2:291293/1‑134 (MQ=255) gcgcTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCG‑CCAGCGCTGGCGGCGCCCGGGCCGCCAGGGTGTGGCAGGCCAACGCCGATAAGGCGCAGCTCGCTGATCAGGCCCATGACTTCGGCAAGCa > 1:294690/1‑136 (MQ=255) gcTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCG‑CCAGCGCTGGCGGCGCCCGGGCCGCCGGGGTGTGGCAGGCCCACGCCGATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGt > 2:110232/1‑136 (MQ=255) gcTGGGGCCCCCCGCCTGGTCGCGGGGGCGCGGCTGGGGGCCG‑CCCGGGGGGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGt < 2:26000/136‑1 (MQ=255) ttGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCG‑CCCGCGCTGGCGGCGCCCGGGCCGCCGGGGTGTGGCAGGCCAACGCCGATAAGGCGCAGCTCGCTGCTCAGGCCGCTGACTTCGGCAAGCAGTTg > 1:40268/1‑136 (MQ=255) ttGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCG‑CCAGCGCTGGCGGCGCCCGGGCCGCCGGGGTGTGGGAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTg > 1:505/1‑136 (MQ=255) ttGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCG‑CCAGCGCTGGCGGCGCCCGGGCAGCCTGGGTGTGGGAGGCCAACGCCGATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCCAGCAGTTg > 2:8342/1‑136 (MQ=255) gggCCCCCCGCCTGGTCGCGGTGGAGCTGCGGGTGGCCG‑CCGGCGCGGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGc < 2:551432/136‑1 (MQ=255) ggCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCG‑CCCGCGCTGGCGGCGCCCGGGCCGCCGGGGTGTGGGAGGCCAACGCCTATAAGGCGCAGCTCGCGGATCCGGCCGATGACTTCGGCAAGCAGTTgggg > 2:125848/1‑134 (MQ=255) ggCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCG‑CCAGCGCTGGCGGCGCCCGGGCAGCCGGGGTGTGGGCGGCCAACGCCGATAAGGCGCCGCTCGCGGATCAGGCCGCTGTCTTCGGCCAGCAGTTgggg > 1:326323/1‑134 (MQ=255) gCCTGGTCGCCGTGGCGCTGCTGCTGGCCG‑CCCGCGCTGGCGGCGCCCGGGCCGCCGGGGTGTGGGAGGCCCACCCCTATAAGGCGCAGCTCGCGGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAggt > 1:232868/1‑135 (MQ=255) gCCTGGTCGCCGTGGCGCCGCTGCTGGCCG‑CCCGCGCGGGCGGCGCCCGGGCAGCCTGGGTGTGGCCGGCCAACGCCGATAAGGCGCCGCTCGCTGATCAGGCCGCTGCCTTCGGCCAGCAGTTGGCGGAGAAggc > 1:496907/1‑135 (MQ=255) gtggcggTGGCGCTGCTGCTGGCCG‑CCAGCGCGGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATcgcg < 1:487722/130‑1 (MQ=255) gTCGCCGTGGCGCTGCTGCTGGCCC‑CCAGCCCTGGCGGCGCCCCGGCCGCCGGGGTGTGGGAGGCCCACCCCTATAAGCGGCCCGACGCGGAAAAGCCCCATGAATAAGGGAACAAGTTGGCGGAGAAGGAAcggg > 2:267198/1‑134 (MQ=255) gcggggTGGCGCTGGTGC‑GCCCGCCCTGCGGGGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATcgcgc < 2:104695/131‑1 (MQ=255) gcGCCGTGGGGCTGCTGCTGGCCG‑CCAGCGGTGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATcgcgc < 1:229809/135‑1 (MQ=255) ggcgggCCG‑CCAGCGCTGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTGGAGCg < 2:306435/132‑1 (MQ=255) gcgggCCG‑CGCGCGCGGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTGGAGCgg < 1:291293/133‑1 (MQ=255) ctgGCCG‑CCTGCGGGGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTGGAGCggg < 2:306250/136‑1 (MQ=255) | CAAACGTATCAATGGTGGTAGCAACGGTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCG‑CCAGCGCTGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTGGAGCGGG > NC_002947/3449125‑3449377 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |