Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,452,733 T→G 6.8% intergenic (‑182/+201) PP_5558 ← / ← PP_3069 Xre family transcriptional regulator/outer membrane autotransporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,452,7330TG 6.8% 194.0 / 7.1 59intergenic (‑182/+201)PP_5558/PP_3069Xre family transcriptional regulator/outer membrane autotransporter
Reads supporting (aligned to +/- strand):  ref base T (25/30);  new base G (2/2);  total (27/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.42e-01

AAGCAATGTATACGGATCAAGGCCTGCTTAGGGGCAGGAGGGTGGTTATTCTAAAAATTATATTATCAGTCGCTAGGTAACCCCAAGGCCATCCTGATCTTTACCGCCTTCCTGCCGCAGTTCGTCTCGGTCGGTAGCAGCACCCCGGTGAGTGAGCAGTTCCTTTGGCTGGGTGTGTTGTTCCTGCTGCTGGAATGGGCGGCCATCGCCATCTACTCAGGCCTGGGCGCCTACATGCAGCGTTGGTTCAGCCAGCCCG  >  NC_002947/3452599‑3452857
                                                                                                                                      |                                                                                                                            
aaGCAATGTATACGGATCAAGGCCTGCTTAGGGGCAGGAGGGTGGTTATTCTAAAAATTATATTATCAGTCGCTAGGTAACCCCAAGGCCATCCTGATCTTTACCGCCTTCCTGCCGCAGTTCGTCTCGGTCGGTa                                                                                                                             <  2:15780/136‑1 (MQ=255)
  gCAATGTATACGGATCAAGGCCTGCTTAGGGGCAGGAGGGTGGTTATTCTAAAAATTATATTATCAGTCGCTAGGTAACCCCAAGGCCATCCTGATCTTTACCGCCTTCCTGCCGCAGTTCGTCTCGGTCGGTagc                                                                                                                           <  1:8091/136‑1 (MQ=255)
    aaTGTATACGGATCAAGGCCTGCTTAGGGGCAGGAGGGTGGTTATTCTAAAAATTATATTATCAGTCGCTAGGTAACCCCAAGGCCATCCTGATCTTTACCGCCTTCCTGCCGCAGTTCGTCTCGGTCGGTagcag                                                                                                                         >  2:461683/1‑136 (MQ=255)
    aaTGTATACGGATCAAGGCCTGCTTAGGGGCAGGAGGGTGGTTATTCTAAAAATTATATTATCAGTCGCTAGGTAACCCCAAGGCCATCCTGATCTTTACCGCCTTCCTGCCGCAGTTCGTCTCGGTCGGTagcag                                                                                                                         >  2:464407/1‑136 (MQ=255)
         ataCGGATCAAGGCCTGCTTAGGGGCAGGAGGGTGGTTATTCTAAAAATTATATTATCAGTCGCTAGGTAACCCCAAGGCCATCCTGATCTTTACCGCCTTCCTGCCGCAGTTCGTCTCGGTCGGTAGCAGCAccc                                                                                                                    >  2:186278/1‑136 (MQ=255)
            cGGATCAAGGCCTGCTTAGGGGCAGGAGGGTGGTTATTCTAAAAATTATATTATCAGTCGCTAGGTAACCCCAAGGCCATCCTGATCTTTACCGCCTTCCTGCCGCAGTTCGTCTCGGTCGGTAGCAGCACCCCgg                                                                                                                 <  1:50069/136‑1 (MQ=255)
                 cAAGGCCTGCTTAGGGGCAGGAGGGTGGTTATTCTAAAAATTATATTATCAGTCGCTAGGTAACCCCAAGGCCATCCTGATCTTTACCGCCTTCCTGCCGCAGTTCGTCTCGGTCGGTAGCAGCACCCCGgtgagt                                                                                                            <  1:401187/136‑1 (MQ=255)
                  aaGGCCTGCTTAGGGGCAGGAGGGTGGTTATTCTAAAAATTATATTATCAGTCGCTAGGTAACCCCAAGGCCATCCTGATCTTTACCGCCTTCCTGCCGCAGTTCGTCTCGGTCGGTAGCAGCACCCCGgtgagtg                                                                                                           <  1:443342/136‑1 (MQ=255)
                  aaGGCCTGCTTAGGGGCAGGAGGGTGGTTATTCTAAAAATTATATTATCAGTCGCTAGGTAACCCCAAGGCCATCCTGATCTTTACCGCCTTCCTGCCGCAGTTCGTCTCGGTCGGTAGCAGCACCCCGgtgagtg                                                                                                           <  2:502060/136‑1 (MQ=255)
                   aGGCCTGCTTAGGGGCAGGAGGGTGGTTATTCTAAAAATTATATTATCAGTCGCTAGGTAACCCCAAGGCCATCCTGATCTTTACCGCCTTCCTGCCGCAGTTCGTCTCGGTCGGTAGCAGCACCCCGgtgagtga                                                                                                          >  2:410139/1‑136 (MQ=255)
                   aGGCCTGCTTAGGGGCAGGAGGGTGGTTATTCTAAAAATTATATTATCAGTCGCTAGGTAACCCCAAGGCCATCCTGATCTTTACCGCCTTCCTGCCGCAGTTCGTCTCGGTCGGTAGCAGCACCCCGgtgagtga                                                                                                          >  2:427162/1‑136 (MQ=255)
                   aGGCCTGCTTAGGGGCAGGAGGGTGGTTATTCTAAAAATTATATTATCAGTCGCTAGGTAACCCCAAGGCCATCCTGATCTTTACCGCCTTCCTGCCGCAGTTCGTCTCGGTCGGTAGCAGCACCCCGgtgagtga                                                                                                          >  1:484611/1‑136 (MQ=255)
                     gCCTGCTTAGGGGCAGGAGGGTGGTTATTCTAAAAATTATATTATCAGTCGCTAGGTAACCCCAAGGCCATCCTGATCTTTACCGCCTTCCTGCCGCAGTTCGTCTCGGTCGGTagc                                                                                                                           >  1:263800/1‑117 (MQ=255)
                     gCCTGCTTAGGGGCAGGAGGGTGGTTATTCTAAAAATTATATTATCAGTCGCTAGGTAACCCCAAGGCCATCCTGATCTTTACCGCCTTCCTGCCGCAGTTCGTCTCGGTCGGTagc                                                                                                                           <  2:263800/117‑1 (MQ=255)
                     gCCTGCTTAGGGGCAGGAGGGTGGTTATTCTAAAAATTATATTATCAGTCGCTAGGTAACCCCAAGGCCATCCTGATCTTTACCGCCTTCCTGCCGCAGTTCGTCTCGGTCGGTAGCAGCACCCCGGTGAGTGAGc                                                                                                        >  2:488741/1‑136 (MQ=255)
                     gCCTGCTTAGGGGCAGGAGGGTGGTTATTCTAAAAATTATATTATCAGTCGCTAGGTAACCCCAAGGCCATCCTGATCTTTACCGCCTTCCTGCCGCAGTTCGTCTCGGTCGGTAGCAGCACCCCGGTGAGTGAGc                                                                                                        >  2:485987/1‑136 (MQ=255)
                            tAGGGGCAGGAGGGTGGTTATTCTAAAAATTATATTATCAGTCGCTAGGTAACCCCAAGGCCATCCTGATCTTTACCGCCTTCCTGCCACAGTTCGTCTCGGTCGGTAGCAGCACCCCGGTGAGTGAGCAGTTCCt                                                                                                 >  1:212091/1‑136 (MQ=255)
                              ggggCAGGAGGGTGGTTATTCTAAAAATTATATTATCAGTCGCTAGGTAACCCCAAGGCCATCCTGATCTTTACCGCCTTCCTGCCGCAGTTCGTCTCGGTCGGTAGCAGCACCCCGGTGAGTGAGCAGTTCCttt                                                                                               <  1:257394/136‑1 (MQ=255)
                              ggggCAGGAGGGTGGTTATTCTAAAAATTATATTATCAGTCGCTAGGTAACCCCAAGGCCATCCTGATCTTTACCGCCTTCCTGCCGCAGTTCGTCTCGGTCGGTAGCAGCACCCCGGTGAGTGAGCAGTTCCttt                                                                                               <  1:186278/136‑1 (MQ=255)
                                 gCAGGAGGGTGGTTATTCTAAAAATTATATTATCAGTCGCTAGGTAACCCCAAGGCCATCCTGATCTTTACCGCCTTCCTGCCGCAGTTCGTCTCGGTCGGTAGCAGCACCCCGGTGAGTCAGCAGTTCCTTTGGc                                                                                            >  2:355400/1‑136 (MQ=255)
                                  cAGGAGGGTGGTTATTCTAAAAATTATATTATCAGTCGCTAGGTAACCCCAAGGCCATCCTGATCTTTACCGCCTTCCTGCCGCAGTTCGTCTCGGTCGGTAGCAGCACCCCGGTGAGTGAGCAGTTCCTTTGGCt                                                                                           >  1:539982/1‑136 (MQ=255)
                                       gtgtggtTATTCTAAAAATTATATTATCAGTCGCTAGGTAACCCCAAGGCCATCCTGATCTTTACCGCCTTCCTGCCGCAGTTCGTCTCGGTCGGTAGCAGCACCCCGGTGAGTGAGCAGTTCCTTTGGCTGGgtg                                                                                      <  2:277048/134‑1 (MQ=255)
                                       gggTGGTTATTCTAAAAATTATATTATCAGTCGCTAGGTAACCCCAAGGCCATCCTGATCTTTACCGCCTTCCTGCCGCAGTTCGTCTCGGTCGGTAGCAGCACCCCGGTGAGTGAGCAGTTCCTTTGGCTGGgtg                                                                                      >  1:214197/1‑136 (MQ=255)
                                             ttATTCTAAAAATTATATTATCAGTCGCTAGGTAACCCCAAGTCCATCCTGAACTTTACCGCCTACCTGCCGCAGTTAGTAACGGTCGGTAGCAGCACCCCGCTGAGTGAGCAGATCCTATGGCTGGGTGtgttgt                                                                                >  1:58991/1‑136 (MQ=255)
                                             ttATTCTAAAAATTATATTATCAGTCGCTAGGTAACCCCAAGGCCATCCTGATCTTTACCGCCTTCCTGCCGCAGTTCGTCTCGGTCGGTAGCAGCACCCCGGTGAGTGAGCAGTTCCTTTGGCTGGGTGtgttgt                                                                                >  1:182251/1‑136 (MQ=255)
                                               aTTCTAAAAATTATATTATCAGTCGCTAGGTAACCCCAAGGCCATCCTGATCTTTACCGCCTTCCTGCCGCAGTTCGTCTCGGTCGGTAGCAGCACCCCGGTGAGTGAGCAGTTCCTTTGGCTGGGTGTGTTGTTc                                                                              <  2:255784/136‑1 (MQ=255)
                                                                  cAGTCGCTAGGTAACCCCAAGGCCATCCTGATCTTTACCGCCTTCCTGCCGCAGTTCGTCTCGGTCGGTAGCAGCACCCCGGTGAGTGAGCAGTTCCTTTGGCTGGGTGTGTTGTTCCTGCTGCTGGAATGggcgg                                                           <  2:539982/136‑1 (MQ=255)
                                                                  cAGTCGCTAGGTAACCCCAAGGCCATCCTGATCTTTACCGCCTTCCTGCCGCAGTTCGTCTCGGTCGGTAGCAGCACCCCGGTGAGTGAGCAGTTCCTTTGGCTGGGTGTGTTGTTCCTGCTGCTGGAATGggcgg                                                           <  2:50674/136‑1 (MQ=255)
                                                                  cAGTCGCTAGGTAACCCCAAGGCCATCCTGATCTTTACCGCCTTCCTGCCGCAGTTCGTCTCGGTCGGTAGCAGCACCCCGGTGAGTGAGCAGTTCCTTTGGCTGGGTGTGTTGTTCCTGCTGCTGGAATGggcgg                                                           <  1:488741/136‑1 (MQ=255)
                                                                    gTCGCTAGGTAACCCCAAGGCCATCCTGATCTTTACCGCCTTCCTGCCGCAGTTCGTCTCGGTCGGTAGCAGCACCCCGGTGAGTGAGCAGTTCCTTTGGCTGGGTGTGTTGTTCCTGCTGCTGGAATGGGCGgcc                                                         <  2:15447/136‑1 (MQ=255)
                                                                       gCTAGGTATCCCCAAGGCCATCCTGATCTTTACCGCCTTGCTGCCGCAGTTCGTCTCGGTCGGTAGCAGCACCCCGGTGAGTGAGCAGTTCCTTTGGCTGGGTGTGTTGTTCCTGCTGCTGGAATGGGCGgccatc                                                      <  1:227779/136‑1 (MQ=255)
                                                                         tAGGTAACCCCAAGGCCATCCTGATCTTTACCGCCTTCCTGCCGCAGTTCGTCTCGGTCGGTAGCAGCACCCCGGTGAGTGAGCAGTTCCTTTGGCTGGGTGTGTTGTTCCTGCTGCTGGAATGGGCGgccatcgc                                                    <  1:227733/136‑1 (MQ=255)
                                                                         tAGGTAACCCCAAGGCCATCCTGATCTTTACCGCCTTCCTGCCGCAGTTCGTCTCGGTCGGTAGCAGCACCCCGGTGAGTGAGCAGTTCCTTTGGCTGGGTGTGTTGTTCCTGCTGCTGGAATGGGCGgccatcgc                                                    <  2:416994/136‑1 (MQ=255)
                                                                         tAGGTAACCCCAAGGCCATCCTGATCTTTACCGCCTTCCTGCCGCAGTTCGTCTCGGTCGGTAGCAGCACCCCGGTGAGTGAGCAGTTCCTTTGGCTGGGTGTGTTGTTCCTGCTGCTGGAATGGGCGgccatcgc                                                    <  1:410139/136‑1 (MQ=255)
                                                                         tAGGTAACCCCAAGGCCATCCTGATCTTTACCGCCTTCCTGCCGCAGTTCGTCTCGGTCGGTAGCAGCACCCCGGTGAGTGAGCAGTTCCTTTGGCTGGGTGTGTTGTTCCTGCTGCTGGAATGGGCGgccatcgc                                                    <  1:34730/136‑1 (MQ=255)
                                                                              aaCCCCAAGGCCATCCTGATCTTTACCGCCTTCCTGCCGCAGTTCGTCTCGGTCGGTAGCAGCACCCCGGTGAGTGAGCAGTTCCTTTGGCTGGGTGTGTTGTTCCTGCTGCTGGAATGGGCGGCCATCGCCATCt                                               <  1:464407/136‑1 (MQ=255)
                                                                              aaCCCCAAGGCCATCCTGATCTTTACCGCCTTCCTGCCGCAGTTCGTCTCGGTCGGTAGCAGCACCCCGGTGAGTGAGCAGTTCCTTTGGCTGGGTGTGTTGTTCCTGCTGCTGGAATGGGCGGCCATCGCCATCt                                               <  1:461683/136‑1 (MQ=255)
                                                                                  ccAAGGCCATCCTGATCTTTACCGCCTTCCTGCCGCAGTTCGTCTCGGTCGGTAGCAGCACCCCGGTGAGTGAGCAGTTCCTTTGGCTGGGTGTGTTGTTCCTGCTGCTGGAATGGGCGGCCATCGCCATCTACTc                                           >  1:215515/1‑136 (MQ=255)
                                                                                        ccATCCTGATCTTTACCGCCTTGCTGCCGCAGTTCGTCTCGGTCGGTAGCAGCACCCCGGTGAGTGAGCTGTTCCTTTGGCTGGGTGTGTTGTTCCTGCTGCTGGAATGGGCGGCCATCGCCATCTACTCAGGCCt                                     <  1:203343/136‑1 (MQ=255)
                                                                                        ccATCCTGATCTTTACCGCCTTCCTGCCGCAGTTCGTCTCGGTCGGTAGCAGCACCCCGGTGAGTGAGCAGTTCCTTTGGCTGGGTGTGTTGTTCCTGCTGCTGGAATGGGCGGCCATCGCCATCTACTCAGGCCt                                     <  1:414595/136‑1 (MQ=255)
                                                                                        ccATCCTGATCTTTACCGCCTTCCTGCCGCAGTTCGTCTCGGTCGGTAGCAGCACCCCGGTGAGTGAGCAGTTCCTTTGGCTGGGTGTGTTGTTCCTGCTGCTGGAATGGGCGGCCATCGCCATCTACTCAGGCCt                                     <  1:382704/136‑1 (MQ=255)
                                                                                        agaTCCTGATCTTTACCGCCTTCCTGCCGCAGTTCGTCTCGGTCGGTAGCAGCACCCCGGTGAGTGAGCAGTTCCTTTGGCTGGGTGTGTTGTTCCTGCTGCTGGAATGGGCGGCCATCGCCATCTACTCAGGCCt                                     <  1:182242/134‑1 (MQ=255)
                                                                                          aTCCTGATCTTTACCGCCTTCCTGCCGCAGTTCGTCTCGGTCGGTAGCAGCACCCCGGTGAGTGAGCAGTTCCTTTGGCTGGGTGTGTTGTTCCTGCTGCTGGAATGGGCGGCCATCGCCATCTACTCAGGCCTgg                                   <  2:552849/136‑1 (MQ=255)
                                                                                          aTCATGATATTTACCGCCTTCCTGCCGCAGTTCGGCTCGGTCGGTAGCAGCACCCCGGTGAGTTAGCAGTTCCTTTGGCTGGGTGTGTTGTTCCTGCTGCTGGAATGGGCGGCCATCGCCATCTACTCAGGCCTgg                                   <  2:67202/136‑1 (MQ=255)
                                                                                              tGATCTTTACCGCCTTCCTGCCGCAGTTCGTCTCGGTCGGTAGCAGCACCCCGGTGAGTGAGCAGTTCCTTTGGCTGGGTGTGTTGTTCCTGCTGCTGGAATGGGCGGCCATCGCCATCTACTCAGGCCTGGgcgc                               >  1:502141/1‑136 (MQ=255)
                                                                                              tGATCTTTACCGCCTTCCTGCCGCAGTTCGTCTCGGTCGGTAGCAGCACCCCGGTGAGTGAGCAGTTCCTTTGGCTGGGTGTGTTGTTCCTGCTGCTGGAATGGGCGGCCATCGCCATCTACTCAGGCCTGGgcgc                               >  2:4870/1‑136 (MQ=255)
                                                                                              tGATCTTTACCGCCTTCCTGCCGCAGTTCGTCTCGGTCGGTAGCAGCACCCCGGTGAGTGAGCAGTTCCTTTGGCTGGGTGTGTTGTTCCTGCTGCTGGAATGGGCGGCCATCGCCATCTACTCAGGCCTGGgcgc                               >  1:195794/1‑136 (MQ=255)
                                                                                              tGATCTTTACCGCCTTCCTGCCGCAGTTCGTCTCGGTCGGTAGCAGCACCCCGGTGAGTGAGCAGTTCCTTTGGCTGGGTGTGTTGTTCCTGCTGCTGGAATGGGCGACCATCGCCATCTACTCAGGCCTGGgcgc                               >  1:467388/1‑136 (MQ=255)
                                                                                              tGATCTTTACCGCCTTCCTGCCGCAGTTCGTCTCGCTCGCTAGCAGCCCCCCGGTGAGTGAGCAGTTCCTTTGGCTGGGTGTGTTGTTCCTGCTGCTGGAATGGGCGGCCATCGCCATCTACTCAGGCCTGGgcgc                               >  1:249258/1‑136 (MQ=255)
                                                                                                   tttACCGCCTTCCTGCAGCAGTTCGTCTCGGTAGGTAGCAGCACACCGGTGAGTGAGCAGTTCCTTTGGCTGGGTGTGTTGTTCCTGCTGATGGAATGGGCGGCAATCGCCATCTAATCAGGACTGGGCGCCTACa                          >  2:106493/1‑136 (MQ=255)
                                                                                                        cGCCTTCCTGCCGCAGTTCGTCTCGGTCGGTAGCAGCACCCCGGTGAGTGAGCAGTTCCTTTGGCTGGGTGTGTTGTTCCTGCTGCTGGAATGGGCGGCCATCGCCATCTACTCAGGCCTGGGCGCCTACATGCAg                     >  1:130909/1‑136 (MQ=255)
                                                                                                        cGCCTTCCTGCCGCAGTTCGTCTCGGTCGGGAGCAGCACCCCGGTGAGTGAGCAGTTCCTTTGGCTGGGTGTGTTGTTCCTGCTGCTGGAATGGGCGGCCATCGCCATCTACTCAGGCCt                                     >  2:408583/1‑120 (MQ=255)
                                                                                                        cGCCTTCCTGCCGCAGTTCGTCTCGGTCGGGAGCAGCACCCCGGTGAGTGAGCAGTTCCTTTGGCTGGGTGTGTTGTTCCTGCTGCTGGAATGGGCGGCCATCGCCATCTACTCAGGCCt                                     >  2:411389/1‑120 (MQ=255)
                                                                                                        cGCCTTCCTGCCGCAGTTCGTCTCGGTCGGGAGCAGCACCCCGGTGAGTGAGCAGTTCCTTTGGCTGGGTGTGTTGTTCCTGCTGCTGGAATGGGCGGCCATCGCCATCTACTCAGGCCt                                     <  1:411389/120‑1 (MQ=255)
                                                                                                        cGCCTTCCTGCCGCAGTTCGTCTCGGTCGGGAGCAGCACCCCGGTGAGTGAGAAGTTCCTTTGGCTGGGTGTGTTGTTCCTGCTGCTGGAATGGGCGGCCATCGCCATCTACTCAGGCCt                                     <  1:408583/120‑1 (MQ=255)
                                                                                                             tCCTGCCGCAGTTCGTCTCGGTCGGTAGCAGCACCCCGGTGAGTGAGCAGTTCCTTTGGCTGGGTGTGTTGTTCCTGCTGCTGGAATGGGCGGCCATCGCCATCTACTCAGGCCTGGGCGCCTACATGCAGCGTTg                >  1:125289/1‑136 (MQ=255)
                                                                                                                tGCCGCAGTTCGTCTCGGTCGGTAGCAGCACCCCGGTGAGTGAGCAGTTCCTTTGGCTGGGTGTGTTGTTCCTGCTGCTGGAATGGGCGGCCATCGCCATCTACTCAGGCCTGGGCGCCTACATGCAGCGTTGGtt             <  1:171799/136‑1 (MQ=255)
                                                                                                                        ttCGTCTCGGTCGGTAGCAGCACCCCGGTGAGTGAGCAGTTCCTTTGGCTGGGTGTGTTGTTCCTGCTGCTGGAATGGGCGGCCATCGCCATCTACTCAGGCCTGGGCGCCTACATGCAGCGTTGGTTcagccagc     >  2:45678/1‑136 (MQ=255)
                                                                                                                           gTCTCGGTCGGTAGCAGCACCCCGGTGAGTGAGCAGTTCCTTTGGCTGGGTGTGTTGTTCCTGCTGCTGGAATGGGCGGCCATCGCCATCTACTCAGGCCTGGGCGCCTACATGCAGCGTTGGTTCAGCCAGCCCg  <  1:26857/136‑1 (MQ=255)
                                                                                                                                      |                                                                                                                            
AAGCAATGTATACGGATCAAGGCCTGCTTAGGGGCAGGAGGGTGGTTATTCTAAAAATTATATTATCAGTCGCTAGGTAACCCCAAGGCCATCCTGATCTTTACCGCCTTCCTGCCGCAGTTCGTCTCGGTCGGTAGCAGCACCCCGGTGAGTGAGCAGTTCCTTTGGCTGGGTGTGTTGTTCCTGCTGCTGGAATGGGCGGCCATCGCCATCTACTCAGGCCTGGGCGCCTACATGCAGCGTTGGTTCAGCCAGCCCG  >  NC_002947/3452599‑3452857

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑