Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,648,276 G→T 11.4% A251A (GCC→GCA)  PP_3213 ← ABC transporter substrate‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,648,2760GT11.4% 106.8 / 4.1 35A251A (GCC→GCA) PP_3213ABC transporter substrate‑binding protein
Reads supporting (aligned to +/- strand):  ref base G (13/18);  new base T (2/2);  total (15/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.71e-01

CCGATCCCCTGGGTTGCAGCCTCTCCACCGGTGACCAGGCGGTACTGCTTGAGGGTCGAGACGCCCCAGGCCAGCAGTTCATCGGTCATGTTGGGGTTGTCGCGCTTGATCAGTGCATTGCCGGCGCTGGGGTCATCCAGATAGCGCTTCCAACCCTCCATGGATGCGCGCACGAAACCTGCTACCCGCTCTGGGTGCTCAGCGATGATGTCTTGACGGGTGACCACGGTGGATCCGTAGGGTGGATAACCGGCATCT  >  NC_002947/3648154‑3648411
                                                                                                                          |                                                                                                                                       
ccGATCCCCTGGGTTGCAGCCTCTCCACCGGTGACCAGGCGGTACTGCTTGAGGGTCGAGACGCCCCAGGCCAGCAGTTCATCGGTCATGTTGGGGTTGTCGCGCTTGATCAGTGCATTGCCGGCGCTGGGGTCAt                                                                                                                            >  2:305117/1‑136 (MQ=255)
    tCCCCTGGGTTGCAGCCTCTCCACCGGTGACCAGGCGGTACTGCTTGAGGGTCGAGACGCCCCAGGCCAGCAGTTCATCGGTCATGTTGGGGTTGTCGCGCTTGATCAGTGCATTGCCGGCGCTGGGGTCATCCAg                                                                                                                        <  2:272387/136‑1 (MQ=255)
            gTTGCAGCCTCTCCACCGGTGACCAGGCGGTACTGCTTGAGGGTCGAGACGCCGCAGGCCAGCAGTTCATCGGTCATGTTGGGGTTGTCGCGCTTGATCAGTGCATTGCCGGCGCTGGGGTCATCCAGATAGCGCt                                                                                                                <  1:251164/136‑1 (MQ=255)
            gTTGCAGCCTCTCCACCGGTGACCAGGCGGTACTGCTTGAGGGTCGAGACGCCCCAGGCCAGCAGTTCATCGGTCATGTTGGGGTTGTCGCGCTTGATCAGTGCATTGCCGGCGCTGGGGTCATCCAGATAGCGCt                                                                                                                <  2:351214/136‑1 (MQ=255)
             ttGCAGCCTCTCCACCGGTGACCAGGCGGTACTGCTTGAGGGTCGAGACGCCCCAGGCCAGCAGTTCATCGGTCATGTT‑GGGTTGTCGCGCTTGATCAGTGCATTGCCGGCGCTGGGGTCATCCAGATAGCGCTTc                                                                                                              <  1:411362/136‑1 (MQ=255)
              tGCAGCCTCTCCACCGGTGACCAGGCGGTACTGCTTGAGGGTCGAGACGCCCCAGGCCAGCAGTTCATCGGTCATGTTGGGGTTGTCGCGCTTGATCAGTGCATTGCCGGCGCTGGGGTCATCCAGATAGCGCTTc                                                                                                              <  1:124251/136‑1 (MQ=255)
                cAGCCTCTCCACCGGTGACCAGGCGGTACTGCTTGAGGGTCGAGACGCCCCAGGCCAGCAGTTCATCGGTCATGTTGGGGTTGTCGCGCTTGATCAGTGCATTGCCGGCGCTGGGGTCATCCAGATAGCGCTTCCa                                                                                                            <  2:312886/136‑1 (MQ=255)
                cAGCCTCTCCACCGGTGACCAGGCGGTACTGCTTGAGGGTCGAGACGCCCCAGGCCAGCAGTTCATCGGTCATGTTGGGGTTGTCGCGCTTGATCAGTGCATTGCCGGCGCTGGGGTCATCCAGATAGCGCTTCCa                                                                                                            <  2:45481/136‑1 (MQ=255)
                   ccTCTCCACCGGTGACCAGGCGGTACTGCTTGAGGGTCGAGACGCCCCAGGCCAGCAGTTCATCGGTCATGTTGGGGTTGTCGCGCTTGATCAGTGCATTGCCGGCGCTGGGGTCATCCAGATAGCGCTTCCAAcc                                                                                                         >  1:407615/1‑136 (MQ=255)
                     tctcCACCGGTGACCAGGCGGTACTGCTTGAGGGTCGAGACGCCCCAGGCCAGCAGTTCATCGGTCATGTTGGGGTTGTCGCGCTTGATCAGTGCATTGCCGGCGCTGGGGTCATCCAGATAGCGCTTCCAACCCt                                                                                                       >  2:114774/1‑136 (MQ=255)
                         cACCGGTGACCAGGCGGTACTGCTTGAGGGTCGAGACGCCCCAGGCCAGCAGTTCATCGGTCATGTTGGGGTTGTCGCGCTTGATCAGTGCATTGCCGGCGCTGGGGTCATCCAGATAGCGCTTCCAACCCTCCAt                                                                                                   >  1:200273/1‑136 (MQ=255)
                            cGGTGACCAGGCGGTACTGCTTGAGGGTCGAGACGCCCCAGGCCAGCAGTTCATCGGTCATGTTGGGGTTGTCGCGCTTGATCAGTGCATTGCCGGCGCTGGGGTCATCCAGATAGCGCTTCCAACCCTCCATGGa                                                                                                <  2:221100/136‑1 (MQ=255)
                                    aGGCGGTACTGCTTGAGGGTCGAGACGCCCCAGGCCAGCAGTTCATCGGTCATGTTGGGGTTGTCGCGCTTGATCAGTGCATTGACGGCGCTGGGGTCATCCAGATAGCGCTTCCAACCCTCCATGGATGCGCGCa                                                                                        >  1:521090/1‑136 (MQ=255)
                                     ggCGGTACTGCTTGAGGGTCGAGACGCCCCAGGCCAGCAGTTCATCGGTCATGTTGGGGTTGTCGCGCTTGATCAGTGCATTGCCGGCGCTGGGGTCATCCAGATAGCGCTTCCAACCCTCCATGGATGCGCGCAc                                                                                       <  1:305117/136‑1 (MQ=255)
                                             tGCTTGAGGGTCGAGACGCCCCAGGCCAGCAGTTCATCGGTCATGTTGGGGTTGTCGCGCTTGATCAGTGCATTGCCGGCGCTGGGGTCATCCAGATAGCGCTTCCAACCCTCCATGGATGCGCGCACGAAACCTg                                                                               <  2:39412/136‑1 (MQ=255)
                                                  gAGGGTCGAGACGCCCCAGGCCAGCAGTTCATCGGTCATGTTGGGGTTGTCGCGCTTGATCAGTGCATTGCCGGCGCTGGGGTCATCCAGATAGCGCTTCCAACCCTCCATGGATGCGCGCACGAAACCTGCTAcc                                                                          >  2:223309/1‑136 (MQ=255)
                                                            aCGCCCCAGGCCAGCAGTTCATCGGTCATGTTGGGGTTGTCGCGCTTGATCAGTGCATTGCCGGCGCTGGGGTCATCCAGATAGCGCTTCCAACCCTCCATGGATGCGCGCACGAAACCTGCTACCCGCTCTGGGt                                                                >  1:17816/1‑136 (MQ=255)
                                                            aCGCCCCAGGCCAGCAGTTCATCGGTCATGTTGGGGTTGTCGCGCTTGATCAGTGCATTGCCGGCGCTGGGGTCATCCAGATAGCGCTTCCAACCCTCCATGGATGCGCGCACGAAACCTGCTACCCGCTCTGGGt                                                                >  1:32084/1‑136 (MQ=255)
                                                                   aGGCCAGCAGTTCATCGGTCATGTTGGGGTTGTCGCGCTTGATCAGTGCATTGCCGGCGCTGGGGTCATCCAGATAGCGCTTCCAACCCTCCATGGATTCGCGCACGAAACCTGCTACCCGCTCTGGGTGCTCAgg                                                         >  2:371922/1‑135 (MQ=255)
                                                                           agTTCATCGGTCATGTTGGGGTTGTCGCGCTTGATCAGTGCATTGCCGGCGCTGGGGTCATCCAGATAGCGCTTCCAACCCTCCATGGATGCGCGCACGAAACCTGCTACCCGCTCTGGGTGCTCAGCGATGATGt                                                 <  1:330565/136‑1 (MQ=255)
                                                                            gTTCATCGGTCATGTTGGGGTTGTCGCGCTTGATCAGTGCATTGCCGGCGCTGGGGTCATCCAGATAGCGCTTCCAACCCTCCATGGATGCGCGCACGAAACCTGCTACCCGCTCTGGGTGCTCAGCGATGATGtg                                                >  1:359341/1‑135 (MQ=255)
                                                                             ttCATCGGTCATGTTGGGGTTGTCGCGCTTGATCAGTGCATTGCCGGCGCTGGGGTCATCCAGATAGCGCTTCCAACCCTCCATGGATGCGCGCACGAAACCTGCTACCCGCTCTGGGTGCTCAGCGATGATGTCt                                               >  2:413484/1‑136 (MQ=255)
                                                                             ttCATCGGTCATGTTGGGGTTGTCGCGCTTGATCAGTGCATTGCCGGCGCTGGGGTCATCCAGAAAGCGCTTCCAACCCTCCATGGATGCGCGCACGAAACCTGCTACCCGCTCTGGGTGCTCAGCGATGATGTCt                                               >  2:265864/1‑136 (MQ=255)
                                                                                     tCATGTTGGGGTTGTCGCGCTTGATCAGTGCATTGCCGGCGCTGGGGTCATCCAGATAGCGCTTCCAACCCTCCATGGATGCGCGCACGAAACCTGCTACCCGCTCTGGGTGCTCAGCGATGATGTCTTGACGGGt                                       <  1:74074/136‑1 (MQ=255)
                                                                                            ggggTTGTCGCGCTTGATCAGTGCATTGCCGGCGCTGGGGTCATCCAGATAGCGCTTCCAACCCTCCATGGATGCGCGCACGAAACCTGCTACCCGCTCTGGGTGCTCAGCGATGATGTCTTGACGGGTGACCACg                                <  1:114774/136‑1 (MQ=255)
                                                                                             gggTTGTCGCGCTTGATCAGTGCATTGCCGGCGCTGGGGTCATCCAGATAGCGCTTCCAACCCTCCATGGATGCGCGCACGAAACCTGCTACCCGCTCTGGGTGCTCAGCGATGATGTCTTGACGGGTGACCACgg                               <  1:335156/136‑1 (MQ=255)
                                                                                                    cgcgCTTGATCAGTGCATTGCCGGCGCTGGGGTCATCCAGATAGCGCTTCCAACCCTCCATGGATGCGCGCACGAAACCTGCTACCCGCTCTGGGTGCTCAGCGATGATGTCTTGACGGGTGACCACGGTGGATcc                        <  2:292140/136‑1 (MQ=255)
                                                                                                           gATCAGTGCATTGCCGGCGCTGGGGTCATCCAGATAGCGCTTCCAACCCTCCATGGATGCGCGCACTAAACGTGCTACCCGCTCTGGGTGCTCAGCGATGATGTCTTGACGTGTGACCACGGTGGATCCGTAGGGt                 <  2:521090/136‑1 (MQ=255)
                                                                                                                   taTTGCCGGCGCTGGGGTCATCCAGATAGCGCTTCCAAGCCTCCATGGATGCGCGCACGAAACCTGCTACCCGCTGTGGGTGCTCAGCGATGATGTCTTGACGGGTGACCACGGTGGATCCGTATGTTTGATAAcc         <  2:342605/135‑1 (MQ=255)
                                                                                                                    aTTGCCTGCGCTGGGGTCATCCAGATAGCGCTTCCAACCCTCCATGGATGCGCGCACGAAACCTGCTACCCGCTCTGGGTGCTCAGCg                                                        <  2:93112/88‑1 (MQ=255)
                                                                                                                    aTTGCCTGCGCTGGGGTCATCCAGATAGCGCTTCCAACCCTCCATGGATGCGCGCACGAAACCTGCTACCCGCTCTGGGTGCTCAGCg                                                        <  2:95641/88‑1 (MQ=255)
                                                                                                                    aTTGCCTGCGCTGGGGTCATCCAGATAGCGCTTCCAACCCTCCATGGATGCGCGCACGAAACCTGCTACCCGCTCTGGGTGCTCAGCg                                                        >  1:95641/1‑88 (MQ=255)
                                                                                                                    aTTGCCTGCGCTGGGGTCATCCAGATAGCGCTTCCAACCCTCCATGGATGCGCGCACGAAACCTGCTACCCGCTCTGGGTGCTCAGCg                                                        >  1:93112/1‑88 (MQ=255)
                                                                                                                    aTTGCCGGCGCTGGGGTCATCCAGATAGCGCTTCCAACCCTCCATGGATGCGCGCACGAAACCTGCTACCCGCTCTGGGTGCTCAGCGATGATGTCTTGACGGGTGACCACGGTGGATCCGTAGGGTGGATAACCg        >  1:311791/1‑136 (MQ=255)
                                                                                                                     ttGCCGGCGCTGGGGTCATCCAGATAGCGCTTCCAACCCTCCATGGATGCGCGCACGAAACCTGCTACCCGCTCTGGGTGCTCAGCGATGATGTCTTGACGGGTGACCACGGTGGATCCGTAGGGTGGATAACCgg       <  1:323126/136‑1 (MQ=255)
                                                                                                                          ggCGCTGGGGTCATCCAGATAGCGCTTCCAACCCTCCATGGATGCGCGCACGAAACCTGCTACCCGCTCTGGGTGCTCAGCGATGATGTCTTGACGGGTGACCACGGTGGATCCGTAGGGTGGATAACCGGCATCt  >  1:146500/1‑136 (MQ=255)
                                                                                                                          |                                                                                                                                       
CCGATCCCCTGGGTTGCAGCCTCTCCACCGGTGACCAGGCGGTACTGCTTGAGGGTCGAGACGCCCCAGGCCAGCAGTTCATCGGTCATGTTGGGGTTGTCGCGCTTGATCAGTGCATTGCCGGCGCTGGGGTCATCCAGATAGCGCTTCCAACCCTCCATGGATGCGCGCACGAAACCTGCTACCCGCTCTGGGTGCTCAGCGATGATGTCTTGACGGGTGACCACGGTGGATCCGTAGGGTGGATAACCGGCATCT  >  NC_002947/3648154‑3648411

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑