| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 221,321 | G→A | 36.4% | E31E (GAG→GAA) | PP_0169 → | TauD/TfdA family dioxygenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 221,321 | 0 | G | A | 36.4% | 5.9 / 6.2 | 11 | E31E (GAG→GAA) | PP_0169 | TauD/TfdA family dioxygenase |
| Reads supporting (aligned to +/- strand): ref base G (3/4); new base A (2/2); total (5/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GTTGCGCGCCATTGAGGTCGCGCATTGCGTGGGAGTGAAAAATGTCAGCCGCCTTGAACGCCTTGTCGTCCATCGACAGCGCCCAGCCGCAAGTTTTTGAAGTTCGCCCCTTCTCGGGTGCCGTCGGCGCCGAGATCTTAGGCCTGGACCTGGCCAAACCGGTCAGCGCCGAGGATTTCACCCGTATCCATCGCGCCCACCTCGACC > NC_002947/221188‑221394 | gTTGCGCGCCATTGAGGTCGCGCATTGCGTGGGAGTGAAAAATGTCAGCCGCCTTGAACGCCTTGTCGTCCATCGACAGCGCCCAGCCGCAAGTTTTTGAAGTTCGCCCCTTCTCGGGTGCCGTCGGCGCCGAGAt > 2:378463/1‑136 (MQ=255) gcgcCATTGAGGTCGCGCATTGCGTGGGAGTGAAAAATGTCAGCCGCCTTGAACGCCTTGTCGTCCATCGACAGCGCCCAGCCGCAAGTTTTTGAAGTTCGCCCCTTCTCGGGTGCCGTCGGCGCCGAGATCTTAg < 1:108399/136‑1 (MQ=255) gcgcCATTGAGGTCGCGCATTGCGTGGGAGTGAAAAATGTCAGCCGCCTTGAACGCCTTGTCGTCCATCGACAGCGCCCAGCCGCAAGTTTTTGAAGTTCGCCCCTTCTCGGGTGCCGTCGGCGCCGAGATCTTAg < 1:486536/136‑1 (MQ=255) cATTGCGTGGGAGTGAAAAATGTCAGCCGCCTTGAACGCCTTGTCGTCCATCGACAGCGCCCAGCCGCAAGTTTTTGAAGTTCGCCCCTTCTCGGGTGCCGTCGGCGCCGAAATCTTAGGCCTg > 1:110177/1‑124 (MQ=255) cATTGCGTGGGAGTGAAAAATGTCAGCCGCCTTGAACGCCTTGTCGTCCATCGACAGCGCCCAGCCGCAAGTTTTTGAAGTTCGCCCCTTCTCGGGTGCCGTCGGCGCCGAAATCTTAGGCCTg > 1:112859/1‑124 (MQ=255) cATTGCGTGGGAGTGAAAAATGTCAGCCGCCTTGAACGCCTTGTCGTCCATCGACAGCGCCCAGCCGCAAGTTTTTGAAGTTCGCCCCTTCTCGGGTGCCGTCGGCGCCGAAATCTTAGGCCTg < 2:110177/124‑1 (MQ=255) cATTGCGTGGGAGTGAAAAATGTCAGCCGCCTTGAACGCCTTGTCGTCCATCGACAGCGCCCAGCCGCAAGTTTTTGAAGTTCGCCCCTTCTCGGGTGCCGTCGGCGCCGAAATCTTAGGCCTg < 2:112859/124‑1 (MQ=255) gTGGGAGTGAAAAATGTCAGCCGCCTTGAACGCCTTGTCGTCCATCGACAGCGCCCAGCCGCAAGTTTTTGAAGTTCGCCCCTTCTCGGGTGCCGTCGGCGCCGAGATCTTAGGCCTGGACCTGGCCAAACCGGTc > 2:126674/1‑136 (MQ=255) gccTTGAACGCCTTGTCGTCCATCGACAGCGCCCAGCCGCAAGTTTTTGAAGTTCGCCCCTTCTCGGGTGCCGTCGGCGCCGAGATCTTAGGCCTGGACCTGGCCAAACCGGTCAGCGCCGAGGATTTCCCCCGtt > 2:531166/1‑135 (MQ=255) cgtcCATCGACAGCGCCCAGCCGCAAGTTTTTGAAGTTCGCCCCTTCTCGGGTGCCGTCGGCGCCGAGATCTTAGGCCTGGACCTGGCCAAACCGGTCAGCGCCGAGGATTTCACCCGTATCCATCGCGCCCACCt < 1:33011/136‑1 (MQ=255) aTCGACAGCGCCCAGCCGCAAGTTTTTGAAGTTCGCCCCTTCTCGGGTGCCGTCGGCGCCGAGATCTTAGGCCTGGACCTGGCCAAACCGGTCAGCGCCGAGGATTTCACCCGTATCCATCGCGCCCACCTCGacc < 1:378463/136‑1 (MQ=255) | GTTGCGCGCCATTGAGGTCGCGCATTGCGTGGGAGTGAAAAATGTCAGCCGCCTTGAACGCCTTGTCGTCCATCGACAGCGCCCAGCCGCAAGTTTTTGAAGTTCGCCCCTTCTCGGGTGCCGTCGGCGCCGAGATCTTAGGCCTGGACCTGGCCAAACCGGTCAGCGCCGAGGATTTCACCCGTATCCATCGCGCCCACCTCGACC > NC_002947/221188‑221394 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |