| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,978,081 | A→C | 32.6% | A251A (GCT→GCG) | PP_3506 ← | magnesium chelatase subunit ChII |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,978,081 | 0 | A | C | 32.6% | 20.2 / 2.1 | 24 | A251A (GCT→GCG) | PP_3506 | magnesium chelatase subunit ChII |
| Reads supporting (aligned to +/- strand): ref base A (5/9); new base C (4/4); total (9/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.62e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GTGGCGCCGACGGCGGGCTGGCCTGGGGCGTCATACGGCGGCGATGGCGCAGGGCGAATTCGGCCACCGCCTCGACATCCACTTCTTCGATAGCTGCAGCCCCACGCCAGGCACAGTGGGCGCGCGCAGCGCGCAGCCACACCAGGTCGGCGCGCAGGCCGTCCACGCCGGCGGCGTAGCAGCGCTCGGTAATCCAGGCAAGCGCCTGGTCATCCAGGGCAATGTCGGCCAAGGCCTGGCGCGCCGCGTGGCAGCGCTCGC > NC_002947/3977954‑3978214 | ggggCCCCCACGGCGGGCTGGCCTGGGGCGTCATACGGCGGCGATGGCGCAGGGCGAATTCGGCCACCGCCTCGACATCCACTTCTTCGATAGCTGCAGCCCCACGCCAGGCACAGTGGGCGcgcgcagcgcgcag < 1:318527/134‑1 (MQ=255) gggcggGCTGGCCTGGGGCGTCATACGGCGGCTTTGGCGCAGGGCTAATTCGCCCACCGCCTCGAAATCCACTTCTTCGATAGCTGCCGCCCCCCGCCAGGCACTGTGGGCGCGCGCAGGGCGCAGCCACACCAgg < 2:275988/135‑1 (MQ=255) gggcggGCTGGCCTGGGGCGTCATACGGCGGCGATGGCGCAGGGCGAATTCGGCCACCGCCTCGACATCCACTTCTTCGATAGCTGCAGCCCCACGCCAGGCACAGTGGGCGCGCGCAGCGCGCAGCCACACCAgg < 2:317288/135‑1 (MQ=255) aTACGGCGGCGATGGCGCAGGGCGAATTCGGCCACCGCCTCGACATCCACTTCTTCGATAGCTGCAGCCCCACGCCAGGCACAGTGGGCGCGCGCAGCGCGCAGCCACACCAGGTCGGCGCGCAGGCCGTCCACGc < 1:241588/136‑1 (MQ=255) aTACGGCGGCGAGGGCGCAGGGCGAATTCGGCCACCGCCTCGACATCCACTTCTTCGATAGCTGCAGCCCCACGCCAGGCACAGTGGGCGCGCGCAGCGCGCAGCCACACCAGGTCGGCGCGCAGGCCGTCCACGc < 2:134486/136‑1 (MQ=255) tACGGCGGCGATGGCGCAGGGCGAATTCGGCCACCGCCTCGACATCCACTTCTTCGATAGCTGCAGCCCCACGCCAGGCACAGTGGGCGCGCGCCCCCCCCCCCCCCACCAGGTCGGCCCGCCCGCCGGCCACGcc > 2:464183/1‑136 (MQ=255) tACGGCGGCGATGGCGCAGGGCGAATTCGGCCACCGCCTCGACATCCACTTCTTCGATAGCTGCAGCCCCACGCCAGGCACAGTGGGCGCGCGCAGCGCGCAGCCACACCAGGTCGGCGCGCAGGCCGTCCAcccc > 2:223833/1‑136 (MQ=255) tACGGCGGCGATGGCGCAGGGCGAATTCGGCCACCGCCTCGACATCCACTTCTTCGATAGCTGCAGCCCCACGCCAGGCACAGTGGGCGCGCGCAGCGCGCAGCCACACCAGGTCGGCGCGCAGGCCGTCCACGcc > 1:164046/1‑136 (MQ=255) cggcggCGAGGCCCCAGGGCCAATCCGCCCACCGCCCCCACACCCACTTCTCCCATCCCGCCCCCCCCCCCCCCGCCACTGGGGGCGCGCGCCGCGCGCAGCCACACCAGGTCGGCGCGCAGGCCGTCCACGCcgg < 2:507629/136‑1 (MQ=255) aTGGCGCAGGGCGAATTCGGCCACCGCCTCGACATCCACTTCTTCGATAGCTGCAGCCCCACGCCAGGCACAGTGGGCGCGCGCCGCGCGCCGCCCCACCAGGTCGGCGCGCACGCCGTCCACGCCGGCGGCGTag > 1:146581/1‑136 (MQ=255) ggCGCAGGGCTAATTCGGCCCCGCCCCCGACATCCACTTCTTCGAACGCTCCAGCCCCCCGCCTGGCACTGTGGGCGCGCGCAGCGCGAAGCCACACCAGGTCGGCGCGCAGGCCGTCCACGCCGGCGGCGTagca < 2:144021/136‑1 (MQ=255) cGAATTCGGCCACCGCCTCGACATCCACTTCTTCGATAGCTGCAGCCCCACGCCAGGCACAGTGGGCGCGCGCAGCGCGCAGCCACACCAGGTCGGCGCGCAGGCCGTCCACGCCGGCGGCGTAGCAGCGCTCGGt < 2:129511/136‑1 (MQ=255) gAATTCGGCCACCGCCTCGACATCCACTTCTTCGATAGCTGCAGCCCCACGCCAGGCACAGTGGGCGCGCGCAGCGCGCACCCCCACCAAGTCCGCGCGCACGCCGTCCACCCCGGCCGCGGAGCAGCGCGCGGTa > 1:139685/1‑136 (MQ=255) aaTTCGGCCACCGCCTCGACATCCACTTCTTCGATAGCTGCAGCCCCAGGCCAGGCACAGTGGGCGCGCGCAGCGCGCAGCCACACCAGGTCGGCGCGCAGGCCGTCCACGCCGGCGGCGTAGCAGCGCTCGGTaa < 1:322296/136‑1 (MQ=255) tCGGCCACCGCCTCGACATCCACTTCTTCGATCGCTGCAGCCCCACGCCAGGCACAGTGGGCGCGCGCAGCGCGCAGCCACACCAGGTCGGCGCGCAGGCCGTCCACGCCGGCGGCGTAGCAGCGCTCGGTAATcc < 2:208959/136‑1 (MQ=255) gcgcccACCGCCTCCACACCCACTTCTGCGATCGAGCCCGCCCCACGCCCGCCACTGGGGGCGGGCGCTGGGCGCAGCCACACCAGGTCGGCGCGCAGGCCGTCCACGCCGGCGGCGTAGCAGCGCTCGGTAATcc < 1:300235/132‑1 (MQ=255) cGGCCCCCGCGTCGACATCCACTTCTTCTATCGCTGCCGCCCCCCGCCCGGCACTGTGGGCGCGCGCCGCGCGCAGCCACACCAGGTCGGCGCGCAGGCCGTCCACGCCGGCGGCGTAGCAGCGCTCGGTAATCCa < 1:442596/136‑1 (MQ=255) cACCGCCTCGACATCCACTTCTTCGATAGCTGCAGCCCCACGCCAGGCACAGTGGGCGCGCGCAGCGCGCAGCCACACCAGGTCGGCGCGCAGGCCGTCCACGCCGGCGGCGTAGCAGCGCTCGGTAATCCAGGCa < 1:140114/136‑1 (MQ=255) aCCGCCTCGACATCCACTTCTTCGATAGCTGCAGCCCCACGCCAGGCACAGTGGGCGCGCGCAGCGCGCCGCCCCACCAGGTCGGCGCGCAGGCCGTCCACGCCGGCGGCGTAGCAGCGCACGGTAATCCAGGCaa > 2:279792/1‑136 (MQ=255) tcttcGATAGCTGCAGCCCCACGCCAGGCACAGTGGGCGCGCGCAGCCCCCCGCCCCACCACGGCGGCGCGCAGGCCGTCCACCCCCGCCGCGTAGCCGCGCACGGTAATACACGCAAGCCCCAGGTCATCCAggg > 2:531045/1‑136 (MQ=255) tcGATAGCTGCCGCCCCACGCCTGGCACAGGGGGCGCGCGCTGCGCGCAGCCACACCAGGTCGGCGCGCAGGCCGTCCACGCCGGCGGCGTAGCAGCGCTCGGTAATCCAGGCAAGCGCCTGGTCATCCAGGGCaa < 2:537320/136‑1 (MQ=255) cctaTAGCGGCGCCCCCCCCCCCGGCACGGGGGGGGCGCCCCGCGCGCAGCCACACCAGGTCGGCGCGCAGGCCGTCCACGCCGGCGGCGTAGCAGCGCTCGGTAATCCAGGCAAGCGCCTGGTCATCCAGGGCaa < 1:216438/133‑1 (MQ=255) cccACGCCAGGCACAGTGGGCGCGCGCCGCGCGCCGCCCCCCCCGGTCGGCGCGCCGGCCGGCCCCGCCGGCGGCGGAGGCGGGCGCGGGTATACAGGCCAGCGCCGGGGGCTCCCGGGCCAAGTCGGGCCAGGcc > 2:338278/1‑136 (MQ=255) cGCCCGGCACCGGGGGCGCGCGCCGCGCGCCGCCACACCAGGGCGGCGCGCAGGCCGTCCACGCCGGCGGCGTAGCAGCGCTCGGTAATCCAGGCAAGCGCCTGGTCATCCAGGGCAATGTCGGCCAAGGCCTGgc < 1:391119/136‑1 (MQ=255) ccAGGCACAGTGGGCGCGGGCCCCGCGCCGCCCCACCCGGGGGGCGCGCCGGGCGGCCACCCCCGCGGCGTTGCCGGGCGGGGGTATACCGGCCAGCGCCGGGTGATCCCGGGCCATGTGGGCCCAGGCCAGgggc > 2:406999/1‑136 (MQ=255) cgcgcgccgcgcgcCGCCACCCCAGGTCGGCGCGCAGGCCGTCCACGCCGGCGGCGTAGCAGCGCGCGGTAATCCAGGCAAGCGCCTGGTCATCCAGGGCAATGTCGGCCAAGGCCTGGCGCGCCGCGTGGCAgcg > 2:482746/1‑136 (MQ=255) gcagcgcgcagcCACACCAGGTCGGCGCGCAGGCCGTCCACGCCGGCGGCGTAGCAGCGCTCGGTAATCCAGGCAAGCGCCTGGTCATCCAGGGCAATGTCGGCCAAGGCCTGGCGCGCCGCGTGGCAGCGCTcgc > 1:520136/1‑136 (MQ=255) | GTGGCGCCGACGGCGGGCTGGCCTGGGGCGTCATACGGCGGCGATGGCGCAGGGCGAATTCGGCCACCGCCTCGACATCCACTTCTTCGATAGCTGCAGCCCCACGCCAGGCACAGTGGGCGCGCGCAGCGCGCAGCCACACCAGGTCGGCGCGCAGGCCGTCCACGCCGGCGGCGTAGCAGCGCTCGGTAATCCAGGCAAGCGCCTGGTCATCCAGGGCAATGTCGGCCAAGGCCTGGCGCGCCGCGTGGCAGCGCTCGC > NC_002947/3977954‑3978214 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |