Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,994,942 A→C 29.3% D31E (GAT→GAG)  PP_3518 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,994,9420AC29.3% 46.8 / 3.2 42D31E (GAT→GAG) PP_3518hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (11/17);  new base C (8/3);  total (22/20)
Fisher's exact test for biased strand distribution p-value = 8.24e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CATCAGCACCTGAGCCAGCAACTGATCAGGGTACAAGGCAATGGGGGCCAGCATCTGGTCCAGTTGCTCCTGGGAGAACACCGGGTCCTTGGCTACGAGCGTGGCAGCGGAAGGCGCCGGATCCGGCGCGGGCACGTCTGCGGCCTGCACCAGGCCAGGGGCAGCGCTCAGGCAAAGCAGTGCCGACAGGAAAGCAAACAATGGCATGCGCATCGCTTATCCCCATGAGGTCAGACCGCGTGGGTTAAGGGT  >  NC_002947/3994822‑3995073
                                                                                                                        |                                                                                                                                   
cATCAGCACCTGAGCCAGTAACTCATCAGGTTACAAGGCTTTGGGGGCCAGGATCTGGTCCAGTTGCTCCTGGGAGAACACCGGGTCCTTGGCTACGAGCGTGGCTGCGGAAGGCGCCGGATCCGGCGCGGGCACg                                                                                                                      <  2:556820/136‑1 (MQ=255)
           gacccAGCAACTGATCAGGGTACAAGGCAATGGGGGCCAGCATCTGGTCCAGTTGCTCCTGGGAGAACACCGGGTCCTTGGCTACGAGCGTGGCAGCGGAAGGCGCCGGATCCGGCGCGGGCACGTCTGCGGCCTg                                                                                                           <  1:551985/133‑1 (MQ=255)
                      tGATCAGGGTACAAGCCAAGGGGGGCCAGCATCTGGTCCAGTTGCTCCTGGGAGAACACCGGGTCCTTGGCCACGAGCGTGGCAGCGGAAGGCGCCGGATCCGGCGCGGGCACGTCTGCGGCCTGCAccaggccag                                                                                                <  1:219595/136‑1 (MQ=255)
                           aGGGTACAAGGCAATGGGGGCCAGCATCTGGTCCAGTTGCTCCTGGGAGAACACCGGGTCCTTGGCTACGAGCGTGGCAGCGGAAGGCGCCGGCTGCGGCGCGGGCCCCTCTGCGGCCGGCGCCCGGCCCGGGGcg                                                                                           >  1:561913/1‑135 (MQ=255)
                            gggTACAAGGCATGGGGGGCCAGCATGTGGTCGTGTTGCTCCTGGGAGAACACCGGGTCCTTGGCTACGCGCGTGGCTGGGGAAGGCGCCGGATCCGGCGCGGGCACGTCTGCGGCCTGCACCAGGCCAGGGGCAg                                                                                          <  2:519151/136‑1 (MQ=255)
                             ggTACAAGGCAATGGGGGCCAGCATCTGGTCCAGTTGCTCCTGGGAGAACACCGGGTCCTTGGCTACGAGCGTGGCAGCGGAAGGCGCCGGATCCCGCGCGGGCACGTCTGCGGCCTGCACCCCGCCAGGGGCCgc                                                                                         >  2:88573/1‑136 (MQ=255)
                                   cggCATTGGGGCCCACGATCTGGTCCAGTTGCTCGTGGGAGAACACCGGGCCCTTGGCTCGGAGCGTGGCTGGGGATGGCGCCGGAGCCGGCGCGGGCACGTCTGCGGCCTGCACCAGGCCAGGGGCAGCGCTCAg                                                                                   <  2:192551/135‑1 (MQ=255)
                                   agccAAGGGGGGCCAGCATCTGGTCCAGTTGCTCCTGGGAGAACACCGGGTCCTTGGCTACGAGCGTGGCAGCGGAAGGCGCCGGATCCGGCGCGGGCACGTCTGCGGCCTGCACCAGGCCAGGGGCAGCGCTCAg                                                                                   <  2:555872/133‑1 (MQ=255)
                                     gCAATGGGGGCCAGCATCTGGTCCAGTTGCTCCTGGGAGAACACCGGGTCCTTGGCTACGAGCGTGGCAGCGGAAGGCGCCGGATCCGGCGCGGGCCCGTCTGCGGCCTGCCCCCGGCCAGGGGCAGCGCGCAGGc                                                                                 >  2:208086/1‑136 (MQ=255)
                                     gCAATGGGGGCCAGCATCTGGTCCAGTTGCTCCTGGGAGAACACCGGGTCCTTGGCTACGAGCGTGGCAGCGGAAGGCGCCGGATCCCGCGCGGGCCCGTCTGCGGCCTGCACCAGGCCCCGGGCAGCGCTCAGGc                                                                                 >  2:365572/1‑136 (MQ=255)
                                          gggggCCAGCATCTGGTCCAGTTGCTCCTGGGAGAACACCGGGTCCTTGGCTACGAGCGTGGCAGCGGAAGGCGCCGGCGCCGGCGCGGGGCCGTCGGCGGCCGGCCCCCGCCCCCGGGCCGCGCGCACGccaccc                                                                            >  1:556497/1‑131 (MQ=255)
                                          gggggCCAGCATCTGGTCCAGTTGCTCCTGGGAGAACACCGGGTCCTTGGCTACGAGCGTGGCAGCGGAAGGCGCCGGATCCGGCGCGGGCACGTCTGCGGCCTGCCCCAGGCCAGGGGCCGCGCGCAGGCAAAGc                                                                            >  2:430196/1‑136 (MQ=255)
                                          gcgggCCAGCATCTGGTCCAGTTGATAATGAGAGAACACCCCGTCCTTGGCAACGAGCGTGGCAGCGGAAGGCGCCGGAAACCGCGCGGGCACGGCTGCCGCCAGCACCACGCCACGGGCACCCCAcaagccaagc                                                                            >  2:220024/3‑136 (MQ=255)
                                            gggCCAGCATCTGGTCCAGTTGCTCCTGGGAGAACACCGGGTCCTTGGCTACGAGCGTGGCAGCGGAAGGCGCCGGGTCCGGCGCGGGCACGTCTGCGGCCGGCCCCAGGCCAGGGGCAGCGCGCCGGCCAAGCAg                                                                          >  2:130813/1‑136 (MQ=255)
                                            gggCCAGCATCTGGTCCAGTTGCTCCTGGGAGAACACCGGGTCCTTGGCTACGAGCGTGGCAGCGGAAGGCGCCGGCTCCGGCGCGGGCCCGGCTGCGGCCGGCCCCCGGCCAGGGGCCGCGCGCAGGCCAAGCAg                                                                          >  2:353953/1‑136 (MQ=255)
                                            gggCCAGCATCTGGTCCAGTTGCTCCTGGGAGAACACCGGGTCCTTGGCTACGAGCGTGGCAGCGGAAGGCGCCGGATCCGGCGCGGGCACGTCTGCGGCCTGCACCAGGCCACGGGCAGCGCTCAGGCAAAGCAg                                                                          >  2:476353/1‑136 (MQ=255)
                                            gggCCAGCATCTGGTCCAGTTGCTCCTGGGAGAACACCGGGTCCTTGGCTACGAGCGTGGCAGCGGAAGGCGCCGGATCCGGCGCCGGCACCTCTGCGGCCTGCACCACGCCCCGGGCAGCGCTCAGGCAAAGCAg                                                                          >  2:477833/1‑136 (MQ=255)
                                                cAGCATCTGGTCCAGTTGCTCCTGGGAGAACACCGGGTCCTTGGCTACGAGCGTGGCAGCGGAAGGCGCCGGCTCCGGCGCGGGCCCCTCAGCGGCCAGCACCACGCCACGGGCAGCCCACAGGCCAAGCAATGcg                                                                      >  1:280427/1‑135 (MQ=255)
                                                 aGCATCTGGTCCAGTTGCTCCTGGGAGAACACCGGGTCCTTGGCTACGAGCGTGGCAGCGGAAGGCGCCGGCTCCGGCGCGGGCACGTCTGCGGCCTGCACCAGGCCAGGGGCAGCGCGCAGGCAAAGCACTGccc                                                                     >  2:131509/1‑135 (MQ=255)
                                                  gCATCTGGTCCAGTTGCTCCTGGGAGAACACCGGGTCCTTGGCTACGAGCGTGGCAGCGGAAGGCGCCGGCTCCGGCGCGGGCCCGGCTGCGGCCGGCACCCGGCCCCGGGCCGCGCGCAGGCCAAGGAGTGCCgc                                                                    >  1:279000/1‑135 (MQ=255)
                                                      cTGGTCCAGTTGCTCCTGGGAGAACACCGGGTCCTTGGCTACGAGCGTGGCAGCGGAAGGCGCCGGATCCGGCGCGGGCACGTCTGCGGCCGGCCCCAGGCCCGGGGCAGCGCTCAGGCAAAGCAGTGCCGACAgg                                                                >  2:561442/1‑136 (MQ=255)
                                                           ccAGTTGCTCCTGGGAGAACACCGGGTCCTTGGCTACGAGCGTGGCAGCGGAAGGCGCCGGGTCCCGCGCGGGCACCTCTGCGGCCTGCACCCCGCCCCGGGGCGCGCGCACGCCAACCAGTGGCGCCACGAAAga                                                           >  2:5559/1‑135 (MQ=255)
                                                             tgTTGCTCCTGGTACACCCCCGGGTCCTTGGCTACGTGGGTGGCTGCGGAAGGCGCCGGAGCCGGCGCGGGCACGTCTGCGGCCTGCACCAGGCCAGGGGCAGCGCTCAGGCAAAGCAGTGCCGACAGGAAAGCaa                                                         <  1:34/135‑1 (MQ=255)
                                                                  ccccTGGCAGAACACCGGGGCGTTGGCTACTCGCGTGGCAGCGGACGCGGCGGTCGCCGGCGCGGGCACGTCTGCGGCCTGCACCAGGCCAGGGGCAGCGCTCAGGCAAAGCAGTGCCGACAGGAAAGCAAACAAt                                                    <  1:261267/134‑1 (MQ=255)
                                                                       gggAGAACCCCGGGTCCTTGGCGACGAGCGTGGCAGCGGAAGGCGCCGGAGCCGGCGCGGGCACGTCTGCGGCCTGCACCAGGCCAGGGGCAGCGCTCAGGCAAAGCATTGCCGACAGGAAAGCAAACAATGGCAt                                               <  2:103920/136‑1 (MQ=255)
                                                                         gttaaCACCGGGTCCCTGGCTACGAGCGTGGCAGCGGAAGGCGCCGGATCCGGCGCGGGCACGTCTGCGGCCTGCACCAGGCCAGGGGCAGCGCTCAGGCAAAGCAGTGCCGACAGGAAAGCAAACAATGGCATgc                                             <  1:504869/133‑1 (MQ=255)
                                                                         cacaaCCCCGGGCCCTTGGCTCCGCGCGTGGCCGCGGACGGCGCCGGAGCCGGCGCGGGCACGTCTGCGGCCTGCACCAGGCCAGGGGCAGCGCTCAGGCAAAGCAGTGCCGACAGGAAAGCAAACAATGGCATgc                                             <  1:561442/133‑1 (MQ=255)
                                                                                ccGGGTCCTTGGCTACGAGCGTGGCAGCGGAAGGCGCCGGCTCCCGCGCGGGCACCTCTGCGGCCTGCACCACGCCAAGGGCCGCGCTCAGGCAAAGCAGTGCCGACAGCAAAGAAAACAATGCCATGCGCATCGc                                      >  2:410496/1‑136 (MQ=255)
                                                                                  gggTCCTTGGCTACGTGGGTGGCAGGGGATGGGGCCGGATCCGGCGCGGGCACGTCTGCGGCCTGCACCAGGCCAGGGGCAGCGCTCAGGCAAAGCAGTGCCGACAGGAAAGCAAACAATGGCATGCGCATCGCtt                                    <  2:538519/136‑1 (MQ=255)
                                                                                      ccTTGGCTACGTGCGGGGCCGCGGATGGCGCCGGCCCCGGCGCGGGCACGTCTGCGGCCTGCACCAGGCCAGGGGCAGCGCTCAGGCAAAGCAGTGCCGACAGGAAAGCAAACAATGGCATGCGCATCGCTTATcc                                <  1:533559/136‑1 (MQ=255)
                                                                                         tGGCTACGAGCGTGGCAGCGGAAGGCGCCGGGTCCGGCGCGGGCCCGTCTGCGGCCGGCCCCCGGCCCGGGGCGGCGCGCCGGCAAAGCAGTGCCGGCAGGAAAGCCAACAATGGCATGCGGCTCGCGTATacccc                             >  2:387400/1‑135 (MQ=255)
                                                                                                  gCGTGGCAGCGGAAGGCGCCGGATCCGGCGCGGGCACGTCTGCGGCCTGCACCAGGCCAGGGGCCGCGCTCAGGCAAAGCAGTGCCGACAGGAAAGCAAACAATTGCATGCGCATCGCTTATCCCCATGAGGTCAg                    >  1:383761/1‑136 (MQ=255)
                                                                                                  gCGTGGCAGCGGAAGGCGCCGGATCCGGCGCGGGCACGTCTGCGGCCTGCACCACGCCAGGGGCAGCGCTCAGGCAAAGCAGTGCCGACAGGAAAGCAAACAATTGCATGCGCATCGCTTATCCCCATGAGGTCAg                    >  1:214405/1‑136 (MQ=255)
                                                                                                     gggCAGCGGCCGGCGCCGGCCCCGGCGCGGGCACGTCTGCGGCCTGCACCAGGCCAGGGGCAGCGCTCAGGCAAAGCAGTGCCGACAGGAAAGCAAACAATGGCATGCGCATCGCTTATCCCCATGAGGTCAGAcc                 <  1:410496/135‑1 (MQ=255)
                                                                                                     gcgCAGCGGACGGCGCCGGAGCCGGCGCGGGCACGTCTGCGGCCTGCACCAGGCCAGGGGCAGCGCTCAGGCAAAGCAGTGCCGACAGGAAAGCAAACAATGGCATGCGCATCGCTTATCCCCATGAGGTCAGAcc                 <  1:88573/134‑1 (MQ=255)
                                                                                                        cAGCGGAAGGCGCCGGCTCCGGCGCGGGGCCCGCTGCGGCCTGCCCCCGGCCAGGGGCAGCGCTCAGGCAAAGCAGTGCCGCCAGGAAAGCAAACAAAGGCATGCGCATCGCGTATACCCATGAGGTGAGACcgcg              >  2:1857/1‑136 (MQ=255)
                                                                                                          gggtagggcggcggcTCCGGCGCGGGCACGTCTGCGGCCTGCACCAGGCCAGGGGCAGCGCTCAGGCAAAGCAGTGCCGACAGGAAAGCAAACAATGGCATGCGCATCGCTTATCCCCATGAGGTCAGACCGCGTg            <  1:5559/130‑1 (MQ=255)
                                                                                                           cgtaaGGCGCCGGATCCGGCGCGGGCACGTCTGCGGCCTGCACCAGGCCAGGGGCAGCGCTCAGGCAAAGCAGTGCCGACAGGAAAGCAAACAATGGCATGCGCATCGCTTATCCCCATGAGGTCAGACCGCGTgg           <  1:487140/133‑1 (MQ=255)
                                                                                                            ggATGGCGCCGGATCGGGCGCGGGCACGTCTGCGGCCTGCACCAGGCCAGGGGCAGCGCTCAGGCAAAGCAGTGCCGACAGGAAAGCAAACAATGGCATGCGCATCGCTTATCCCCATGAGGTCAGACCGCGTggg          <  1:188222/136‑1 (MQ=255)
                                                                                                            ggAAGGCGCCGGATCCGCCGCGGGCACGTCTGCGGCCTGCACCAGGCCAGGGGCAGCGCTCAGGCAAAGCAGTGCCGACAGGAAAGCAAACAATGGCATGCGCATCGCTTATCCCCATGAGGTCAGACCGCGTggg          <  1:46424/136‑1 (MQ=255)
                                                                                                              aaGGCGCCGGATACGGCGCGGGCCCGTCTGCGGCCTGCACCAGGCCAGGGGCAGCGCTCAGGCAAAGCAGTGCCGACAGGAAAGCAAACAATGGCATGCGCATCGCGAATACCCATGAGGTCAGACCGCGGGGGtt        >  2:326285/1‑136 (MQ=255)
                                                                                                                    ccGGAGCCGGCGCGGGCACGTCTGCGGCCTGCACCAGGCCAGGGGCAGCGCTCAGGCAAAGCAGTGCCGACAGGAAAGCAAACAATGGCATGCGCATCGCTTATCCCCATGAGGTCAGACCGCGTGGGTTAAGGGt  <  2:44725/136‑1 (MQ=255)
                                                                                                                    ccGGAGCCGGCGCGGGCACGTCTGCGGCCTGCACCAGGCCAGGGGCAGCGCTCAGGCAAAGCAGTGCCGACAGGAAAGCAAACAATGGCATGCGCATCGCTTATCCCCATGAGGTCAGACCGCGTGGGTTAAGGGt  <  1:131509/136‑1 (MQ=255)
                                                                                                                        |                                                                                                                                   
CATCAGCACCTGAGCCAGCAACTGATCAGGGTACAAGGCAATGGGGGCCAGCATCTGGTCCAGTTGCTCCTGGGAGAACACCGGGTCCTTGGCTACGAGCGTGGCAGCGGAAGGCGCCGGATCCGGCGCGGGCACGTCTGCGGCCTGCACCAGGCCAGGGGCAGCGCTCAGGCAAAGCAGTGCCGACAGGAAAGCAAACAATGGCATGCGCATCGCTTATCCCCATGAGGTCAGACCGCGTGGGTTAAGGGT  >  NC_002947/3994822‑3995073

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑