| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,419,305 | C→T | 25.0% | L78L (CTG→TTG) | PP_3905 → | LuxR family transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,419,305 | 0 | C | T | 25.0% | 31.2 / 4.9 | 16 | L78L (CTG→TTG) | PP_3905 | LuxR family transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base C (7/5); new base T (2/2); total (9/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GGTTCATGGCAGCGAGCGTCACGACCAATGCAGCCCACCCCCGAGATCAAATCATGGACACGATCACTATCGGCGCATGGATAGGCCATCTCGGCCGAGGCCTGGCGCCTCGCGAACTGCAATGCCTGTTGGAAGTAGCCCAGGGCTTCACCACCAAGGAAATCGCCAAGCACTTCGGCATCAGCGAGAGCGGCGTAGAGAAACGCATCGGTGACGCAATGCTGAAGCTCAAGGTTGCCCGGCGCGCCG > NC_002947/4419189‑4419437 | ggTTCATGGCAGCGAGCGTCACGACCAATGCAGCCCACCCCCGAGATCAAATCATGGACACGATCACTATCGGCGCATGGATAGGCCATCTCGGCCGAGGCCTGGCGCCTCGCGAACTGCAATGCCTGTTGGAAGt > 1:109329/1‑136 (MQ=255) gTTCATGGCAGCGAGCGTCACGACCAATGCAGCCCACCCCCGAGATCAAATCATGGACACGATCACTATCGGCGCATGGATAGGCCATCTCGGCCGAGGCCTGGCGCCTCGCGAACTGCAATGCCTGTTGGAAGTa < 2:490973/136‑1 (MQ=255) aTGGCAGCGAGCGTCACGACCAATGCAGCCCACCCCCGAGATCAAATCATGGACACGATCACTATCGGCGCATGGATAGGCCATCTCGGCCGAGGCCTGGCGCCTCGCGAACTGCAATGCCTGTTGGAAGTAGccc > 2:326840/1‑136 (MQ=255) gCAGCCCA‑CACCGAGATCAAA‑AATGGACACGATCACTATCGGCGCATGGATAGGCCACCTCGGCCGAGGCCTGGCGCCTCGCGAATTGCAATGCCTGTTGGAAGTAGCCCAGGGCTTCACCACCAAGGAAATCGcc > 1:277724/1‑136 (MQ=14) gCAGCCCA‑CACCGAGATCAAA‑AATGGACACGATCACTATCGGCGCATGGATAGGCCACCTCGGCCGAGGCCTGGCGCCTCGCGAATTGCAATGCCTGTTGGAAGTAGCCCAGGGCTTCACCACCAAGGAAATCGcc > 1:512614/1‑136 (MQ=14) cGAGATCAAATCATGGACACGATCACTATCGGCGCATGGATAGGCCATCTCGGCCGAGGCCTGGCGCCTCGCGAACTGCAATGCCTGTTGGAAGTAGCCCAGGGCTTCACCACCAAGGAAATCGCCAAGCACTTCg < 1:411078/136‑1 (MQ=255) gagaTCAAA‑AATGGACACGATCACTATCGGCGCATGGATAGGCCACCTCGGCCGAGGCCTGGCGCCTCGCGAATTGCAATGCCTGTTGGAAGTAGCCCAGGGCTTCACCACCAAGGAAATCGCCAAGCACTTCGGc < 2:277724/136‑1 (MQ=18) gagaTCAAA‑AATGGACACGATCACTATCGGCGCATGGATAGGCCACCTCGGCCGAGGCCTGGCGCCTCGCGAATTGCAATGCCTGTTGGAAGTAGCCCAGGGCTTCACCACCAAGGAAATCGCCAAGCACTTCGGc < 2:512614/136‑1 (MQ=18) aaTCGGCGCATGGATAGGCCATCTCGGCCGAGGCCTGGCGCCTCGCGAACTGCAATGCCTGTTGGAAGTAGCCCAGGGCTTCACCACCAAGGAAATCGCCAAGCACTTCGGCATCAGCGAGAGCGGCGTAGAGaaa < 1:192484/135‑1 (MQ=255) aTGGATAGGCCATCTCGGCCGAGGCCTGGCGCCTCGCGAACTGCAATGCCTGTTGGAAGTAGCCCAGGGCTTCACCACCAAGGAAATCGCCAAGCACTTCGGCATCAGCGAGAGCGGCGTAGAGAAACGCATCGGt > 2:27662/1‑136 (MQ=255) aTAGGCCATCTCGGCCGAGGCCTGGCGCCTCGCGAACTGCAATGCCTGTTGGAAGTAGCCCAGGGCTTCACCACCAAGGAAATCGCCAAGCACTTCGGCATCAGCGAGAGCGGCGTAGAGAAACGCATCGGTGACg > 1:565355/1‑136 (MQ=255) aTAGGCCATCTCGGCCGAGGCCTGGCGCCTCGCGAACTGCAATGCCTGTTGGAAGTAGCCCAGGGCTTCACCACCAAGGAAATCGCCAAGCACTTCGGCATCAGCGAGAGCGGCGTAGAGAAACGCATCGGTGACg > 2:253519/1‑136 (MQ=255) gCCATCTCGGCCGAGGCCTGGCGCCTCGCGAACTGCAATGCCTGTTGGAAGTAGCCCAGGGCTTCACCACCAAGGAAATCGCCAAGCACTTCGGCATCAGCGAGAGCGGCGTAGAGAAACGCATCGGTGACGCAAt < 2:347262/136‑1 (MQ=255) ccATCTCGGCCGAGGCCTGGCGCCTCGCGAACTGCAATGCCTGTTGGAAGTAGCCCAGGGCTTCACCACCAAGGAAATCGCCAAGCACTTCGGCATCAGCGAGAGCGGCGTAGAGAAACGCATCGGTGACGCAATg < 1:383639/136‑1 (MQ=255) cGGCCGAGGCCTGGCGCCTCGCGAACTGCAATGCCTGTTGGAAGTAGCCCAGGGCTTCACCACCAAGGAAATCGCCAAGCACTTCGGCATCAGCGAGAGCGGCGTAGAGAAACGCATCGGTGACGCAATGCTGAAg > 1:27418/1‑136 (MQ=255) gAACTGCAATGCCTGTTGGAAGTAGCCCAGGGCTTCACCACCAAGGAAATCGCCAAGCACTTCGGCATCAGCGAGAGCGGCGTAGAGAAACGCATCGGTGACGCAATGCTGAAGCTCAAGGTTGCCCGGCGcgccg > 1:533607/1‑136 (MQ=255) | GGTTCATGGCAGCGAGCGTCACGACCAATGCAGCCCACCCCCGAGATCAAATCATGGACACGATCACTATCGGCGCATGGATAGGCCATCTCGGCCGAGGCCTGGCGCCTCGCGAACTGCAATGCCTGTTGGAAGTAGCCCAGGGCTTCACCACCAAGGAAATCGCCAAGCACTTCGGCATCAGCGAGAGCGGCGTAGAGAAACGCATCGGTGACGCAATGCTGAAGCTCAAGGTTGCCCGGCGCGCCG > NC_002947/4419189‑4419437 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |