Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	441,304	A→C	43.1%	Q61P (CAG→CCG)	bioF →	8‑amino‑7‑oxononanoate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	441,304	0	A	C	43.1%	6.4 / 3.0	16	Q61P (CAG→CCG)	bioF	8‑amino‑7‑oxononanoate synthase
Reads supporting (aligned to +/- strand):  ref base A (4/5);  new base C (2/5);  total (6/10)
Fisher's exact test for biased strand distribution p-value = 6.33e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGTATCGGCAGCGGCCATTGCTGGAAAGCCCGCAAGGGCCGGAAGTGGTGGTCGACGGCCAGCGGTTGCTGGCCTTCTGCAGCAATGACTACCTGGGCCTGGCCAACCACCCCGAGGTGATCGCCGCCTGGCAGGCCGGCGCCGAGCGCTGGGGTGTGGGGGGGGGGG‑CCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATCAGGTTGAAGAGGCACTGGCTGAACTCACCGGCCGCCCGCGCGCCCTGTT  >  NC_002947/441172‑441427                                                                                                                                     |                                                                                                                             tGTATCGGCAGCGGCCATTGCTGGAAAGCCCGCAAGGGCCGGAAGTGGTGGTCGACGGCCCGCCGGTGCTGGGCTTATGCCGCAATGACGAACAGGGGCTGGGCCACCACCCCGAGGTGTTAGCCCCCAGGCCGGc                                                                                                                           >  1:505430/1‑136 (MQ=255) tGTATCGGCAGCGGCCATTGCTGGAAAGCCCGCAAGGGCCGGAAGTGGTGGTCGACGGCCCCCCGTTGCGGGGCGTGAGGGGCCATGACGACCAGGGCCAGGGCCACCCCCCCCCGGTGATCGCCCCCAGGCCGGc                                                                                                                           >  2:490609/1‑136 (MQ=255)     tCGGCAGCGGCCATTGCTGGAAAGCCCGCAAGGGCCGGAAGTGGTGGTCGACGGCCAGCGGTTGCTGGCCTTCTGCAGCAATGACTACCTGGGCCTGGCCAACCACCCCGAGGTGATCGCCGCCTGGCAGGCCGgc                                                                                                                       <  1:455335/136‑1 (MQ=255)     tCGGCAGCGGCCATTGCTGGAAAGCCCCCAAGGGCCGGAAGTGGTGGTCGACGGCCAGCGGTTGCTGGCCTTCTGCAGCAATGACTACCTGGGCCTGGCCAACCACCCCGAGGTGATCGCCGCCTGGCAGGCCGgc                                                                                                                       <  1:424714/136‑1 (MQ=255)      cGGCAGCGGCCATTGCTGGAAAGCCCGCAAGGGCCGGAAGTGGTGGTCGACGGCCCGCGGTTTCTGGGCGTGTGGAGCAATGACTACCAGGGCCTGGCCAACCCCCCCCAGGTGATCGCCGCCAGGCAGGCCCgcc                                                                                                                      >  2:311011/1‑135 (MQ=255)                    gCTGGACACCCCGCACGGGCCGGAAGGGGGGGGCGCCGGCCCGCGGTTGCTGGCCTTCTGCACCAATGCCTCCCTGGGCCTGGCCAACCCCCCCGAGGTGATCCCCGCCGGGCAGGCCGGCGCCGCGCGCGggggg                                                                                                        <  2:396721/136‑2 (MQ=255)                                     ggCCGGAAGTGGTGGTCGACGGCCAGCGGTTGCTGGCCTTCTGCCGCAATGACTACCTGGGCCTGGCCAACCACCCCGAGGTGATCGCCGCCGGGCAGGCCGGCGCCGCGCGGTGGGGGGTGGGGGGGGGGG‑cggg                                                                                      >  1:133833/1‑133 (MQ=255)                                            aGTGGGGGGCGACGGCCCGCGGTGGGTGGCCTTCTGCACCAATGCCTCCCCGGGCCCGGCCAACCACCCCGAGGGTCTCGCCGCCTGGCCCGCCGGCGCCGCGCGCGGGGGGGGGGGGGGGGGGG‑CCTCGCACCTg                                                                               <  2:524640/136‑1 (MQ=255)                                                      caCGGCCAGCGGTTGCGGGCCTTCTGCAGCAATGACTACCTGGGCCGGGCCAACCACCCCGAGGTGATCGCCGCCTGGCCGGCCGGCGCCGAGCGCTGGGGTGTGGGGGGGGGGG‑CCTCGCACCTGGTAGTTGGcc                                                                     <  2:143071/135‑1 (MQ=255)                                                                   ggCTGGCCTGCCGCACCAATTACTACCTGGGCCGGGCCAACCCCCCCGACGGTCTCCCCCCCGGGCCGGCCGGCGCCGCCGGCCGGGGGGTGGGGGGGGGGG‑CCTCGCACCTGGTAGTTGGCCACAGCACCCCGCa                                                        <  2:528738/135‑1 (MQ=255)                                                                                    aaTGCCTACCTGGGCAGTGCCAACCTCCCCGCGGGTTTCGCCGCCTGGCAGGCCGGGGCCGCCGGGGGGGGGGGGGGGGGGGGGG‑CCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATCAGGTTGAAGAgg                                       <  2:219429/136‑1 (MQ=255)                                                                                       ttatACCTGGGCCTGGCCAACCCCCCGGGGGTGATCGCCGGCTGGGAGGGCGGCGGCGAGGGCGGGGGGGGGGGGGGGGGGG‑CCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATCAGGTTGAAGAGGCAc                                    <  1:301413/133‑1 (MQ=255)                                                                                          ccccggggCCCGCCCACCCCCCCCGCGGGGACCGCCCCCGGGCCGGCCGGCGCCGCGCGGGGGGGGGGGGGGGGGGGGG‑CCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATCAGGTTGAAGAGGCACTgg                                 <  1:191139/131‑1 (MQ=255)                                                                                                ggCCTCGCCAACCACCCCGAGGTGATCGCCGCCTGGCAGGCCGGCGCCGAGCGCTGGGGTGTGGGGGGGGGGG‑CCTCGCACCTGGTAGTTGGCCACAGCCCCCCGCCCCATCAGGTTGAAGAGGCACTGGCTGAAc                           >  2:383463/1‑136 (MQ=255)                                                                                                              ccccGAGGTGATCGCCGCCTGGCAGGCCGGCGCCGAGCGCTGGGGTGTGGGGGGGGGGGCCCTCGCCCCGGGAAGTTGCCCCCACCACCCCGCCCAATCCGGGTCAAGCGGCAGTGGGTACACACACCgcccgccc              >  1:169446/1‑136 (MQ=255)                                                                                                                         tCGCCGCCGGGCCGGCCGGCGCCGTGCGGGGGGGGGTGGGGGGGGGGG‑CCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATCAGGTTGAAGAGGCACTGGCTGAACTCACCGGCCGCCCGCGCGCCCtgtt  <  1:385896/136‑1 (MQ=255)                                                                                                                                     |                                                                                                                             TGTATCGGCAGCGGCCATTGCTGGAAAGCCCGCAAGGGCCGGAAGTGGTGGTCGACGGCCAGCGGTTGCTGGCCTTCTGCAGCAATGACTACCTGGGCCTGGCCAACCACCCCGAGGTGATCGCCGCCTGGCAGGCCGGCGCCGAGCGCTGGGGTGTGGGGGGGGGGG‑CCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATCAGGTTGAAGAGGCACTGGCTGAACTCACCGGCCGCCCGCGCGCCCTGTT  >  NC_002947/441172‑441427

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑