Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,498,593 C→A 11.8% C21F (TGC→TTC)  cobG ← precorrin‑3B synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,498,5930CA11.8% 107.1 / 5.5 34C21F (TGC→TTC) cobGprecorrin‑3B synthase
Reads supporting (aligned to +/- strand):  ref base C (9/21);  new base A (2/2);  total (11/23)
Fisher's exact test for biased strand distribution p-value = 5.80e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.69e-01

GATCACGCCGCCAGCAAAACGCTCCGCCGCCGCCGCCACGGCTTCAGCCTGCTCGGCCAGCAGCAGGCCACCCGGCAGCTTGATGCGGCAGATACCGCCATCACGGGCACTGACGATGCGCCACAACCCCGGGCAGGCCGAGGGACGAGGGATGACAGTGGGGGCTTCGGCCTGGGTCAATGGGGTGTCCGGCAATCTGTGAAAGTGCGCTTGAGTGTAGAAGGCGCGGTATTATGCCTGCTTTGTCCGGCGGCATG  >  NC_002947/5498460‑5498716
                                                                                                                                     |                                                                                                                           
gATCACGCCGCCAGCAAAACGCTCCGCCGCCGCCGCCACGGCTTCAGCCTGCTCGGCCAGCAGCAGGCCACCCGGCAGCTTGATGCGGCAGATACCGCCATCACGGGCACTGACGATGCGCCACAACCCCGGGCAg                                                                                                                           <  1:483293/136‑1 (MQ=255)
        cgccAGCAAAACGCTCCGCCGCCGCCGCCACGGCTTCAGCCTGCTCGGCCAGCAGCAGGCCACCCGGCAGCTTGATGCGGCAGATACCGCCATCACGGGCACTGACGATGCGCCACAACCCCGGGCAGGCcgaggg                                                                                                                   <  2:313252/136‑1 (MQ=255)
        cgccAGCAAAACGCTCCGCCGCCGCCGCCACGGCTTCAGCCTGCTCGGCCAGCAGCAGGCCACCCGGCAGCTTGATGCGGCAGATACCGCCATCACGGGCACTGACGATGCGCCACAACCCCGGGCAGGCcgaggg                                                                                                                   <  2:8860/136‑1 (MQ=255)
           cAGCAAATCGCTCCGCCGCCGCCGCCACGGCTTCAGCCTGCTCGGCCAGCAGCAGGCCACCGGGCAGCTTGATGCGGCAGATACCGCCATCACGGGCACTGACGATGGGCGACAACCCCTGGCAGTCcgagggacg                                                                                                                <  2:24686/136‑1 (MQ=255)
           cAGCAAAACGCTCCGCCGCCGCCGCCACGGCTTCAGCCTGCTCGGCCAGCAGCAGGCCACCCGGCAGCTTGATGCGGCAGATACCGCCATCACGGGCACTGACGATGCGCCACAACCCCGGGCAGGCcgagggacg                                                                                                                <  2:329683/136‑1 (MQ=255)
                 aaCGCTCCGCCGCCGCCGCCACGGCTTCAGCCTGCTCGGCCAGCAGCAGGCCACCCGGCAGCTTGATGCGGCAGATACCGCCATCACGGGCACTGACGATGCGCCACAACCCCGGGCAGGCCGAGGGACGAGGGAt                                                                                                          <  2:527344/136‑1 (MQ=255)
                     cTCCGCCGCCGCCGCCACGGCTTCAGCCTGCTCGGCCAGCAGCAGGCCACCCGGCAGCTTGATGCGGCAGATACCGCCATCACGGGCACTGACGATGCGCCACAACCCCGGGCAGGCCGAGGGACGAGGGATGACa                                                                                                      <  1:287597/136‑1 (MQ=255)
                      tCCGCCGCCGCCGCCACGGCTTCAGCCTGCTCGGCCAGCAGCAGGCCACCCGGCAGCTTGATGCGGCAGATACCGCCATCACGGGCACTGACGATGCGCCACAACCCCGGGCAGGCCGAGGGACGAGGGATGACAg                                                                                                     <  2:210913/136‑1 (MQ=255)
                      gccgccgccgccgCCACGGCTTCAGCCTGCTCGGCCAGCAGCAGGCCACCCGGCAGCTTGATGCGGCAGATACCGCCATCACGGGCACTGACGATGCGCCACAACCCCGGGCAGGCCGAGGGACGAGGGATGACAg                                                                                                     <  2:530150/135‑1 (MQ=255)
                                   ccACGGCTTCAGCCTGCTCGGCCAGCAGCAGGCCACCCGGCAGCTTGATGCGGCAGATACCGCCATCACGGGCACTGACGATGCGCCACAACCCCGGGCAGGCCGAGGGACGAGGGATGACAGTGGGGGCTTCGGc                                                                                        <  2:467981/136‑1 (MQ=255)
                                     aCGGCTTCAGCCTGCTCGGCCAGCAGCAGGCCACCCGGCAGCTTGATGCGGCAGATACCGCCATCACGGGCACTGACGATGCGCCACAACCCCGGGCAGGCCGAGGGACGAGGGATGACAGTGGGGGCTTCGGCCt                                                                                      >  2:260114/1‑136 (MQ=255)
                                             aGCCTGCTCGGCCAGCAGCAGGCCACCCGGCAGCTTGATGCGGCAGATACCGCCATCACGGGCACTGACGATGCGCCACAACCCCGGGCAGGCCGAGGGACGAGGGATGACAGTGGGGGCTTCGGCCTGGGTCAAt                                                                              <  2:85841/136‑1 (MQ=255)
                                             aGCCTGCTCGGCCAGCAGCAGGCCACCCGGCAGCTTGATGCGGCAGATACCGCCATCACGGGCACTGACGATGCGCCACAACCCCGGGCAGGCCGAGGGACGAGGGATGACAGTGGGGGCTTCGGCCTGGGTCAAt                                                                              <  2:511634/136‑1 (MQ=255)
                                             aGCCTGCTCGGCCAGCAGCAGGCCACCCGGCAGCTTGATGCGGCAGATACCGCCATCACGGGCACTGACGATGCGCCACAACCCCGGGCAGGCCGAGGGACGAGGGATGACAGTGGGGGCTTCGGCCTGGGTCAAt                                                                              <  2:470247/136‑1 (MQ=255)
                                             aGCCTGCTCGGCCAGCAGCAGGCCACCCGGCAGCTTGATGCGGCAGATACCGCCATCACGGGCACTGACGATGCGCCACAACCCCGGGCAGGCCGAGGGACGAGGGATGACAGTGGGGGCTTCGGCCTGGGTCAAt                                                                              <  2:452013/136‑1 (MQ=255)
                                                      ggCCAGCAGCAGGCCACCCGGCAGCTTGATGCGGCAGATACCGCCATCACGGGCACTGACGATGCGCCACAACCCCGGGCAGGCCGAGGGACGAGGGATGACAGTGGGGGCTTCGGCCTGGGTCAATGGGGTGTcc                                                                     >  1:83312/1‑136 (MQ=255)
                                                      ggCCAGCAGCAGGCCACCCGGCAGCTTGATGCGGCAGATACCGCCATCACGGGCACTGACGATGCGCCACAACCCCGGGCAGGCCGAGGGACGAGGGATGACAGTGGGGGCTTCGGCCTGGGTCAATGGGGTGTcc                                                                     >  1:111615/1‑136 (MQ=255)
                                                                        cGGCAGCTTGATGCGGCAGATACCGCCATCACGGGCACTGACGATGCGCCACAACCCCGGGCAGGCCGAGGGACGAGGGATGACAGTGGGGGCTTCGGCCTGGGTCAATGGGGTGTCCGGCAATCTGTGAAAGTgc                                                   <  2:404314/136‑1 (MQ=255)
                                                                          gCAGCTTGATGCGGCAGATACCGCCATCACGGGCACTGACGATGCGCCACAACCCCGGGCAGGCCGAGGGACGAGGGATGACAGTGGGGGCTTCGGCCTGGGTCAATGGGGTGTCCGGCAATCTGTGAAAGTgcgc                                                 <  2:35079/136‑1 (MQ=255)
                                                                               ttGATGCGGCAGATACCGCCATCACGGGCACTGACGATGCGCCACAACCCCGGGCAGGCCGAGGGACGAGGGATGACAGTGGGGGCTTCGGCCTGGGTCAATGGGGTGTCCGGCAATCTGTGAAAGTGCGCTTGAg                                            >  1:123161/1‑136 (MQ=255)
                                                                                       gCAGATACCTCCATCACGGGCACTGACGATGCGCCACAACCCCTGGCAGGCCGAGTGACGAGGGATGACAGTGGGGGCTTCGGCCTGGGTCAATGGTGTGTCCGGCAATCTGTGAAAGTGCGCTTGAGTGTAGaat                                    <  1:354050/136‑2 (MQ=255)
                                                                                       gCAGATACCGCCATCACGGGCACTGACGATGCGCCACAACCCCGGGCAGGCCGAGGGACGAGGGATGACAGTGGGGGCTTCGGCCTGGGTCAATGGGGTGTCCGGCAATCTGTGAAAGTGCGCTTGAGTGTAGAAg                                    <  2:307329/136‑1 (MQ=255)
                                                                                            aaCCGCCATCACGGGCACTGACGATGCGCCACAACCCCGGGCAGGCCGAGGGACGAGGGATGACAGTGGGGGCTTCGGCCTGGGTCAATGGGGTGTCCGGCAATCTGTGAAAGTGCGCTTGAGTGTAGAAGGcgcg                               <  2:298086/135‑1 (MQ=255)
                                                                                              ccGCCATCACGGGCACTGACGATGCGCCACAACCCCGGGAAGGCCGAGGGACGAGGGATGACAGTGGGGGCTTCGGCCTGGGTCAATGGGGTGTCCGGCAATCTGTGAAAGTGCGCTTGAGTGTAGAAg                                    >  2:289502/1‑129 (MQ=255)
                                                                                              ccGCCATCACGGGCACTGACGATGCGCCACAACCCCGGGAAGGCCGAGGGACGAGGGATGACAGTGGGGGCTTCGGCCTGGGTCAATGGGGTGTCCGGCAATCTGTGAAAGTGCGCTTGAGTGTAGAAg                                    >  2:286602/1‑129 (MQ=255)
                                                                                              ccGCCATCACGGGCACTGACGATGCGCCACAACCCCGGGAAGGCCGAGGGACGAGGGATGACAGTGGGGGCTTCGGCCTGGGTCAATGGGGTGTCCGGCAATCTGTGAAAGTGCGCTTGAGTGTAGAAg                                    <  1:289502/129‑1 (MQ=255)
                                                                                              ccGCCATCACGGGCACTGACGATGCGCCACAACCCCGGGAAGGCCGAGGGACGAGGGATGACAGTGGGGGCTTCGGCCTGGGTCAATGGGGTGTCCGGCAATCTGTGAAAGTGCGCTTGAGTGTAGAAg                                    <  1:286602/129‑1 (MQ=255)
                                                                                                       cGGGCACTGACGATGCGCCACAACCCCGGGCAGGCCGAGGGACGAGGGATGACAGTGGGGGCTTCGGCCTGGGTCAATGGGGTGTCCGGCAATCTGTGAAAGTGCGCTTGAGTGTAGAAgg                                   >  2:505525/1‑121 (MQ=255)
                                                                                                       cGGGCACTGACGATGCGCCACAACCCCGGGCAGGCCGAGGGACGAGGGATGACAGTGGGGGCTTCGGCCTGGGTCAATGGGGTGTCCGGCAATCTGTGAAAGTGCGCTTGAGTGTAGAAgg                                   <  1:505525/121‑1 (MQ=255)
                                                                                                       cGGGCACTGACGATGCGCCACAACCCCGGGCAGGCCGAGGGACGAGGGATGACAGTGGGGGCTTCGGCCTGGGTCAATGGGGTGTCCGGCAATCTGTGAAAGTGCGCTTGAGTGTAGAAGGCGCGGTATTATGCCt                    <  1:29236/136‑1 (MQ=255)
                                                                                                            aCTGACGATGCGCCACAACCCCGGGCAGGCCGAGGGACGAGGGATGACAGTGGGGGCTTCGGCCTGGGTCAATGGGGTGTCCGGCAATCTGTGAAAGTGCGCTTGAGTGTAGAAGGCGCGGTATTATGCCTGCttt               >  2:436137/1‑136 (MQ=255)
                                                                                                                 cGATGCGCCACAACCCCGGGCAGGCCGAGGGACGAGGGATGACAGTGGGGGCTTCGGCCTGGGTCAATGGGGTGTCCGGCAATCTGTGAAAGTGCGCTTGAGTGTAGAAGGCGCGGTATTATGCCTGCTTTGTCcg          >  2:86112/1‑136 (MQ=255)
                                                                                                                       gcCACAACCCCGGGCAGGCCGAGGGACGAGGGATGACAGTGGGGGCTTCGGCCTGGGTCAATGGGGTGTCCGGCAATCTGTGAAAGTGCGCTTGAGTGTAGAAGGCGCGGTATTATGCCTGGTTTGTCCggcggcg    >  1:70894/1‑135 (MQ=255)
                                                                                                                         cacaACCCCGGGCAGGCCGAGGGACGAGGGATGACAGTGGGGGCTTCGGCCTGGGTCAATGGGGTGTCCGGCAATCTGTGAAAGTGCGCTTGAGTGTAGAAGGCGCGGTATTATGCCTGCTTTGTCCGGCGGCATg  >  2:215979/1‑136 (MQ=255)
                                                                                                                                     |                                                                                                                           
GATCACGCCGCCAGCAAAACGCTCCGCCGCCGCCGCCACGGCTTCAGCCTGCTCGGCCAGCAGCAGGCCACCCGGCAGCTTGATGCGGCAGATACCGCCATCACGGGCACTGACGATGCGCCACAACCCCGGGCAGGCCGAGGGACGAGGGATGACAGTGGGGGCTTCGGCCTGGGTCAATGGGGTGTCCGGCAATCTGTGAAAGTGCGCTTGAGTGTAGAAGGCGCGGTATTATGCCTGCTTTGTCCGGCGGCATG  >  NC_002947/5498460‑5498716

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑