| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,671,943 | 0 | T | C | 41.7% | ‑2.0 / 10.4 | 12 | A780A (GCA→GCG) | acnB | aconitate hydratase B |
| Reads supporting (aligned to +/- strand): ref base T (2/5); new base C (2/3); total (4/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.43e-01 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GGCAGTTTGCCGATGATCGAAGCGACAGCGGCCAGCTCGGCCGATGCCAGGTACACGTTGGTCGCGTCGCCCAGACGGTTCGGGAAGTTACGGGTCGAGGTGGAGACCACGGTCGAACCGGTCTGCACGCGTGCCTGGTTGCCCATGCACAGCGAGCAGCCTGGCATTTCCATACGTGCACCGGCCTTGCCGTAGATGCCGTAGTAGCCTTCTTCGGTCAGCTGGTGAGCGTCCATCTTGGTTGGCGG > NC_002947/2671812‑2672059 | ggCAGTTTGCCGATGATCGAAGCGACAGCGGCCAGCTCGGCCGATGCCAGGTACACGTTGGTCGCGTCGCCCAGACGGTTCGGGAAGTTACGGGTCGAGGTGGAGACCACGGTCGAACCGGTCTGCACGCGTGCCt < 1:3059/136‑1 (MQ=255) gCAGTTTGCCGATGATCGAAGCGACAGCGGCCAGCTCGGCCGATGCCAGGTACACGTTGGTCGCGTCGCCCAGACGGTTCGGGAAGTTACGGGTCGAGGTGGAGACCACGGTCGAACCGGTCTGCACGCGTGCCTg > 1:127148/1‑136 (MQ=255) ggTCGCGTCGCCCAGACGGTTCGGGAAGTTACGGGGCGAGGTGGAGACCACGGTCGAACCGGTCTGCACGCGTGCCTGGTTGCCCATGCACAGCGAGCAGCCTGGCATTTCCATACGTGCACCGGCCTTGCCGTAg < 2:409279/136‑1 (MQ=255) cGGTTCGGGAAGTTACGGGTCGAGGTGGAGACCACGGTCGAACCGGTCTGCACGCGTGCCTGGTTGCCCATGCACAGCGAGCAGCCTGGCATTTCCATACGTGCACCGGCCTTGCCGTAGATGCCGTAGTAGCctt < 1:26415/136‑1 (MQ=255) gggTAGAGGTCGAAACCACCGTTGCACCGTCCGCCACACGCGCCTGGTTACCCATACACAGGGAACAGCCAGGGATCTCGATACGCGCACCACTCTTACCGAAGACGCTGTAGTAGCCTTCTTCGGTCAACTgtgc < 2:434611/136‑4 (MQ=255) ccACGGTCGAACCGGTCTGCACGCGTGCCTGGTTGCCCATGCACAGCGAGCAGCCTGGCATTTCCATACGTGCACCGGCCTTGCCGTAGATGCCGTAGTAGCCttcttc > 1:196175/1‑109 (MQ=255) ccACGGTCGAACCGGTCTGCACGCGTGCCTGGTTGCCCATGCACAGCGAGCAGCCTGGCATTTCCATACGTGCACCGGCCTTGCCGTAGATGCCGTAGTAGCCttcttc < 2:196175/109‑1 (MQ=255) ccGAACCGGTCTGCACCCGTGCCTGGATGCCCATGCACAGCGAGCAGCCTGGCATTTCCATACGTGCACCGGCCTTGCCGTAGATGCCGTAGTAGCCTTCTTCGGTCAGCTGGTGAGCGTCCATCTTGGTTGGCgg < 2:432596/135‑1 (MQ=255) cacaCGCGCCTGGTTACCCATACACAGGGAACAGCCAGGGATCTCGATACGCGCACCACTCTTACCGAAGACGCTGTAGTAGCCTTCTTCGGTCAACTGTGCGGCGTCCATACGGGTTGGCgg < 1:28964/123‑1 (MQ=255) cacaCGCGCCTGGTTACCCATACACAGGGAACAGCCAGGGATCTCGATACGCGCACCACTCTTACCGAAGACGCTGTAGTAGCCTTCTTCGGTCAACTGTGCGGCGTCCATACGGGTTGGCgg < 1:31032/123‑1 (MQ=255) cacaCGCGCCTGGTTACCCATACACAGGGAACAGCCAGGGATCTCGATACGCGCACCACTCTTACCGAAGACGCTGTAGTAGCCTTCTTCGGTCAACTGTGCGGCGTCCATACGGGTTGGCgg > 2:28964/1‑123 (MQ=255) cacaCGCGCCTGGTTACCCATACACAGGGAACAGCCAGGGATCTCGATACGCGCACCACTCTTACCGAAGACGCTGTAGTAGCCTTCTTCGGTCAACTGTGCGGCGTCCATACGGGTTGGCgg > 2:31032/1‑123 (MQ=255) | GGCAGTTTGCCGATGATCGAAGCGACAGCGGCCAGCTCGGCCGATGCCAGGTACACGTTGGTCGCGTCGCCCAGACGGTTCGGGAAGTTACGGGTCGAGGTGGAGACCACGGTCGAACCGGTCTGCACGCGTGCCTGGTTGCCCATGCACAGCGAGCAGCCTGGCATTTCCATACGTGCACCGGCCTTGCCGTAGATGCCGTAGTAGCCTTCTTCGGTCAGCTGGTGAGCGTCCATCTTGGTTGGCGG > NC_002947/2671812‑2672059 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |