| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,671,976 | 0 | C | G | 33.3% | 18.2 / 10.8 | 15 | M769I (ATG→ATC) | acnB | aconitate hydratase B |
| Reads supporting (aligned to +/- strand): ref base C (6/4); new base G (2/3); total (8/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.08e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GGTCGCGTCGCCCAGACGGTTCGGGAAGTTACGGGTCGAGGTGGAGACCACGGTCGAACCGGTCTGCACGCGTGCCTGGTTGCCCATGCACAGCGAGCAGCCTGGCATTTCCATACGTGCACCGGCCTTGCCGTAGATGCCGTAGTAGCCTTCTTCGGTCAGCTGGTGAGCGTCCATCTTGGTTGGCGGAGCCAGCCACAGACGGGTCGGAATGCCACCCTTGACCTTCTCCAGCAGCTT > NC_002947/2671871‑2672110 | ggTCGCGTCGCCCAGACGGTTCGGGAAGTTACGGGGCGAGGTGGAGACCACGGTCGAACCGGTCTGCACGCGTGCCTGGTTGCCCATGCACAGCGAGCAGCCTGGCATTTCCATACGTGCACCGGCCTTGCCGTAg < 2:409279/136‑1 (MQ=255) cGGTTCGGGAAGTTACGGGTCGAGGTGGAGACCACGGTCGAACCGGTCTGCACGCGTGCCTGGTTGCCCATGCACAGCGAGCAGCCTGGCATTTCCATACGTGCACCGGCCTTGCCGTAGATGCCGTAGTAGCctt < 1:26415/136‑1 (MQ=255) gggTAGAGGTCGAAACCACCGTTGCACCGTCCGCCACACGCGCCTGGTTACCCATACACAGGGAACAGCCAGGGATCTCGATACGCGCACCACTCTTACCGAAGACGCTGTAGTAGCCTTCTTCGGTCAACTgtgc < 2:434611/136‑4 (MQ=255) ccACGGTCGAACCGGTCTGCACGCGTGCCTGGTTGCCCATGCACAGCGAGCAGCCTGGCATTTCCATACGTGCACCGGCCTTGCCGTAGATGCCGTAGTAGCCttcttc > 1:196175/1‑109 (MQ=255) ccACGGTCGAACCGGTCTGCACGCGTGCCTGGTTGCCCATGCACAGCGAGCAGCCTGGCATTTCCATACGTGCACCGGCCTTGCCGTAGATGCCGTAGTAGCCttcttc < 2:196175/109‑1 (MQ=255) ccGAACCGGTCTGCACCCGTGCCTGGATGCCCATGCACAGCGAGCAGCCTGGCATTTCCATACGTGCACCGGCCTTGCCGTAGATGCCGTAGTAGCCTTCTTCGGTCAGCTGGTGAGCGTCCATCTTGGTTGGCgg < 2:432596/135‑1 (MQ=255) cacaCGCGCCTGGTTACCCATACACAGGGAACAGCCAGGGATCTCGATACGCGCACCACTCTTACCGAAGACGCTGTAGTAGCCTTCTTCGGTCAACTGTGCGGCGTCCATACGGGTTGGCgg < 1:28964/123‑1 (MQ=255) cacaCGCGCCTGGTTACCCATACACAGGGAACAGCCAGGGATCTCGATACGCGCACCACTCTTACCGAAGACGCTGTAGTAGCCTTCTTCGGTCAACTGTGCGGCGTCCATACGGGTTGGCgg < 1:31032/123‑1 (MQ=255) cacaCGCGCCTGGTTACCCATACACAGGGAACAGCCAGGGATCTCGATACGCGCACCACTCTTACCGAAGACGCTGTAGTAGCCTTCTTCGGTCAACTGTGCGGCGTCCATACGGGTTGGCgg > 2:28964/1‑123 (MQ=255) cacaCGCGCCTGGTTACCCATACACAGGGAACAGCCAGGGATCTCGATACGCGCACCACTCTTACCGAAGACGCTGTAGTAGCCTTCTTCGGTCAACTGTGCGGCGTCCATACGGGTTGGCgg > 2:31032/1‑123 (MQ=255) cATGCACAGCGAGCAGCCTGGCATTTCCATACGTGCACCGGCCTTGCCGTAGATGCCGTAGTAGCCTTCTTCGGTCAGCTGGTGAGCGTCCATCTTGGTTGGCGGAGCCAGCCACAGACGGGTCGGAATGCCAccc > 1:433951/1‑136 (MQ=255) gcagcCTGGCATTTCCATACGTGCACCGGCCTTGCCGTAGATGCCGTAGTAGCCTTCTTCGGTCAGCTGGTGAGCGTCCATCTTGGTTGGCGGAGCCAGCCACAGACGGGTCGGAATGCCACCCTTGACCTTCTcc > 1:172564/1‑136 (MQ=255) gcagcCTGGCATTTCCATACGTGCACCGGCCTTGCCGTAGATGCCGTAGTAGCCTTCTTCGGTCAGCTGGTGAGCGTCCATCTTGGTTGGCGGAGCCAGCCACAGACGGGTCGGAATGCCACCCTTGACCTTCTcc > 1:280272/1‑136 (MQ=255) ccTGGCATTTCCATACGTGCACCGGCCTTGCCGTAGATGCCGTAGTAGCCTTCTTCGGTCAGCTGGTGAGCGTCCATCTTGGTTGGCGGAGCCAGCCACAGACGGGTCGGAATGCCACCCTTTACCTTCTCcagcc > 1:176572/1‑135 (MQ=255) gCATTTCCATACGTGCACCGGCCTTGCCGTAGATGCCGTAGTAGCCTTCTTCGGTCAGCTGGTGAGCGTCCATCTTGGTTGGCGGAGCCAGCCACAGACGGGTCGGAATGCCACCCTTAACCTTCTCCAACAACtt > 1:173627/1‑136 (MQ=255) | GGTCGCGTCGCCCAGACGGTTCGGGAAGTTACGGGTCGAGGTGGAGACCACGGTCGAACCGGTCTGCACGCGTGCCTGGTTGCCCATGCACAGCGAGCAGCCTGGCATTTCCATACGTGCACCGGCCTTGCCGTAGATGCCGTAGTAGCCTTCTTCGGTCAGCTGGTGAGCGTCCATCTTGGTTGGCGGAGCCAGCCACAGACGGGTCGGAATGCCACCCTTGACCTTCTCCAGCAGCTT > NC_002947/2671871‑2672110 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |