Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029476,072,1380AC36.0% 52.3 / 13.8 50V162G (GTG→GGG) PP_5324two‑component system response regulator
Reads supporting (aligned to +/- strand):  ref base A (8/24);  new base C (18/0);  total (26/24)
Fisher's exact test for biased strand distribution p-value = 9.40e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

AACGCCTTGATCACACATGGCTGCATGCCACTGGCCAGGCGCAGCTGGCCCTGGCCACGGCCGCTCGGCGCGGGGGCGGCAGGTGCGGGCGCCGCGACCAAAGGCTTGGCCGGTGCCGGTGTCGGCGCCGCCACCTTGACCGCTTCGGGCTTGCCCGCAGTCAGCGCGTTCAACGAGTCGTTGGCAAAGGCCGGGTTGGCTCGCGCCGGGCCGATGGCCATCAGGCTGTCGAGCTTACCGATCTTGGTCAGG  >  NC_002947/6072006‑6072257
                                                                                                                                    |                                                                                                                       
atcgcgTTGATCACACATGGCGGCTGGCCTCTGGCCTGGCGCTGGTGGCCGTGGCCACGGCCGCTCGGCGCGGGGGCGGCAGGTGCGGGCGCCGCGACCAAAGGCTTGGCCGGTGCCGGTGTCGGCGCCGCCACCt                                                                                                                      <  1:178752/134‑1 (MQ=255)
 aCGCCTTGATCACACATGGCTGCATGCCACTGGCCAGGCGCAGCTGGCCCTGGCCACGGCCGCTCGGCGCGGGGGCGGCAGGTGCGGGCGCCGCGACCAAAGGCTTGGCCGGTGCCGGTGTCGGCGCCGCCACCtt                                                                                                                     <  1:384764/136‑1 (MQ=255)
 aCGCCTTGATCACACATGGCTGCATGCCACTGGCCAGGCGCAGCGGGCCCTGGCCACGGCCGCTCGGCGCGGGGGCGGCAGGTGCGGGCGCCGCGACCAAAGGCTTGGCCGGTGCCGGTGTCGGCGCCGCCACCtt                                                                                                                     <  1:207968/136‑1 (MQ=255)
   gCCTTGATCACACATGGCTGCATGCCACTGGCCAGGCGCAGCTGGCCCTGGCCACGGCCGCTCGGCGCGGGGGCGGCAGGTGCGGGCGCCGCGACCAAAGGCTTGGCCGGTGCCGGTGTCGGCGCCGCCACCt                                                                                                                      <  2:79743/133‑1 (MQ=255)
   gCCTTGATCACACATGGCTGCATGCCACTGGCCAGGCGCAGCTGGCCCTGGCCACGGCCGCTCGGCGCGGGGGCGGCAGGTGCGGGCGCCGCGACCAAAGGCTTGGCCGGTGCCGGTGTCGGCGCCGCCACCt                                                                                                                      >  1:79743/1‑133 (MQ=255)
   gCCTTGATCACACATGGCTGCATGCCACTGGCCAGGCGCAGCTGGCCCTGGCCACGGCCGCTCGGCGCGGGGGCGGCAGGTGCGGGCGCCGCGACCAAAGGCTTGGCCGGTGCCGGTGTCGGCGCCGCCACCTTGa                                                                                                                   >  2:253813/1‑136 (MQ=255)
   gCCTTGATCACACATGGCTGCATGCCACTGGCCAGGCGCAGCTGGCCCTGGCCACGGCCGCTCGGCGCGGGGGCGGCAGGTGCGGGCGCCGCGACCAAAGGCTTGGCCGGTGCCGGTGTCGGCGCCGCCACCATGa                                                                                                                   >  2:322707/1‑136 (MQ=255)
        gATCACACATGGCTGCATGCCACTGGCCAGGCGCAGCTGGCCCTGGCCACGGCCGCTCGGCGCGGGGGCGGCAGGTGCGGGCGCCGCGACCAAAGGCTTGGCCGGTGCCGGTGTCGGCGCCGCCACCTTGACCGCt                                                                                                              <  2:376826/136‑1 (MQ=255)
            acacaTGGCTGCAGGCCACTGGCCTGGCGCAGCGGGCCCTGGCCACGGCCGCTCGGCGCGGGGGCGTCAGGTGCGGGCTCCGCGACCAAAGGCTTGGCCGGTGCCGGTGTCGGCGCCGCCACCTTGACCGCTTCgg                                                                                                          <  1:238828/136‑1 (MQ=255)
                aTGGCTGCATGCCACTGGCCAGGCGCAGCTGGCCCTGGCCACGGCCGCTCGGCGCGGGGGCGGCAGGTGCGGGCGCCGCGACCAAAGGCTTGGCCGGTGCCGGTGTCGGCGCCGCCCCCGTGGCCGCTGCGGGcgt                                                                                                      >  1:254118/1‑134 (MQ=255)
                aTGGCGGCAAGCCACTGGCCAGGCGCAGCTGGCCCTGGCCACGGCCGCTCGGCGCGGGGGCGGCAGGTGCGGGCGCCGCGACCAAAGGCTTGGCCGGTGCCGGTGTCGGCGCCGCCACCTTGACCGCTTCGGGCtt                                                                                                      <  1:276330/136‑1 (MQ=255)
                 tGGCTGCATGCCACTGGCCAGGCGCAGCTGGCCCTGGCCACGGCCGCTCGGCGCGGGGGCGGCAGGTGCGGGCGCCGCGACCAAAGGCTTGGCCGGTGCCGGTGTCGGCGCCGCCACCATGACCGCGTCGGGCTTg                                                                                                     >  2:216437/1‑136 (MQ=255)
                     tGCATGCCACTGGCCAGGCGCAGCTGGCCCTGGCCACGGCCGCTCGGCGCGGGGGCGGCAGGTGCGGGCGCCGCGACCAAAGGCTTGGCCGGTGCCGGTGTGGGCGCCGCCCCCTAGCCCGCGTCGGGCATGgccc                                                                                                 >  1:419481/1‑135 (MQ=255)
                          gCCACTGGCCAGGCGCAGCTGGCCCTGGCCACGGCCGCTCGGCGCGGGGGCGGCAGGTGCGGGCGCCGCGACCAAAGGCTTGGCCGGTGCCGGTGTGGGCGGCGCCCCCTTGACCGCGGCGGGCGTGCCCGCAGTc                                                                                            >  1:438419/1‑136 (MQ=255)
                          gCCACTGGCCAGGCGCAGCTGGCCCTGGCCACGGCCGCTCGGCGCGGGGGCGGCAGGTGCGGGCGCCGCGACCAAAGGCTTGGCCGGTGCCGGTGTCGGCGCCGCCACCATGACCGCTTCGGGCTTGCCCGCAGTc                                                                                            >  1:294128/1‑136 (MQ=255)
                          gCCACTGGCCAGGCGCAGCTGGCCCTGGCCACGGCCGCTCGGCGCGGGGGCGGCAGGTGCGGGCGCCGCGACCAAAGGCTTGGCCGGTGCCGGGGTCGGCGCCGCCCCCGTGACCGCGTCGGGCTTGCACGCAgag                                                                                            >  2:276330/1‑134 (MQ=255)
                                  ccAGGCGCAGCTGGCCCTGGCCACGGCCGCTCGGCGCGGGGGCGGCAGGTGCGGGCGCCGCGACCAAAGGCTTGGCCGGTGCCGGTGTGGGCGCCGCCCCCTTGGCCGCGTCGGGCGTGCCCGCCGTCCGCGCGtg                                                                                    >  2:329097/1‑135 (MQ=255)
                                  ccAGGCGCAGCTGGCCCTGGCCACGGCCGCTCGGCGCGGGGGCGGCAGGTGCGGGCGCCGCGACCAAAGGCTTGGCCGGTGCCGGTGTCGGCGCCGCCCCCATGACCGCGTCGGGCGTGCCCGCAGGCcgcgcggt                                                                                    >  2:108966/1‑134 (MQ=255)
                                   cAGGCGCAGCTGGCCCTGGCCACGGCCGCTCGGCGCGGGGGCGGCAGGTGCGGGCGCCGCGACCAAAGGCTTGGCCGGTGCCGGTGTCGGCGCCGCCACCTTGACCGCTTCGGGCTTGCCCGCAGTCAGCGCGTTc                                                                                   <  2:362660/136‑1 (MQ=255)
                                         cAGCTGGCCCTGGCCACGGCCGCTCGGCGCGGTGGCGGCAGGTGCGGGCGCCGCGACCAAAGGCTTGGCCGGTGCCGGTGTCGGCGCCGCCCCCCTGACCGCGTCGGGGTTGCGCGCAGTCAGCGCGTTCAACGAg                                                                             >  2:238665/1‑136 (MQ=255)
                                         cAGCTGGCCCTGGCCACGGCCGCTCGGCGCGGGGGCGGCAGGTGCGGGCGCCGCGACCAAAGGCTTGGCCGGTGCCGGTGTCGGCGCCGCCCCCTTGACCGCGGCGGGCGTGCCCGCAGGCAGCGCGGTGAACGAg                                                                             >  2:245947/1‑136 (MQ=255)
                                         cAGCTGGCCCTGGCCACGGCCGCTCGGCGCGGGGGCGGCAGGTGCGGGCGCCGCGACCAAAGGCTTGGCCGGTGCCGGTGTCGGCGCCGCCCCCATGACCGCTTGGGGCTTGCCCGCAGTCAGCGCGGTGAACGAg                                                                             >  2:214764/1‑136 (MQ=255)
                                         cAGCTGGCCCTGGCCACGGCCGCTCGGCGCGGGGGCGGCAGGTGCGGGCGCCGCGACCAAAGGCTTGGCCGGTGCCGGTGTCGGCGCCGCCACCTTGACCGCTTCGGGCTTGCCCGCAGTCAGCGCGTTCAACGAg                                                                             >  1:320314/1‑136 (MQ=255)
                                         cAGCTGGCCCTGGCCACGGCCGCTCGGCGCGGGGGCGGCAGGTGCGGGCGCCGCGACCAAAGGCTTGGCCGGTGCCGGTGTCGGCGCCGCCACCGTGACCGCGTCGGGCTTGCCCGCAGTCAGCGCGGTCAACgcc                                                                             >  1:232337/1‑134 (MQ=255)
                                         cAGCTGGCCCTGGCCACGGCCGCTCGGCGCGGGGGCGGCAGGTGCGGGCGCCGCGACCAAAGGCTTGGCCGGTGCCGGGGTGGGCGCCGCCCCCGTGCCCGCTTCGGGCTTGCCCGCCGTCAGCGCGTTCCACGAg                                                                             >  1:261502/1‑136 (MQ=255)
                                              ggccctggccACGGCCGCTCGGCGCGGGGGCGGCAGGTGCGGGCGCCGCGACCAAAGGCTTGGCCGGTGCCGGTGTCGGCGCCGCCCCCGAGCCCGCGTCGGGCGTGCCCGCAGGCAGCGCGGTCAACGAGTCGtt                                                                        >  1:47706/1‑136 (MQ=255)
                                               gccctggccACGGCCGCTCGGCGCGGGGGCGGCAGGTGCGGGCGCCGCGACCAAAGGCTTGGCCGGTGCCGGTGTCGGGGCCGCCCCCGTGCCCGCTGCGGGCGTGGCCGGCGTCCGCGCGTTGCAAGAGTCGtgg                                                                       >  1:409764/1‑134 (MQ=255)
                                               gccctggccACGGCCGCTCGGCGCGGGGGCGGCAGGTGCGGGCGCCGCGACCAAAGGCTTGGCCGGTGCCGGTGTCGGCGCCGCCACCTTGACCGCTTCGGGCTTGCCCGCAGTCAGCGCGTTCAACGAGTCGTTg                                                                       <  1:422551/136‑1 (MQ=255)
                                               gccctggccACGGCCGCTCGGCGCGGGGGCGGCAGGTGCGGGCGCCGCGACCAAAGGCTTGGCCGGTGCCGGTGTCGGCGCCGCCACCTTGACCGCTTCGGGCTTGCCCGCAGTCAGCGCGTTCAACGAGTCGTTg                                                                       <  1:366048/136‑1 (MQ=255)
                                               gccctggccACGGCCGCTCGGCGCGGGGGCGGCAGGTGCGGGCGCCGCGACCAAAGGCTTGGCCGGTGCCGGTGTCGGCGCCGCCACCTTGACCGCTTCGGGCTTGCACGCAGTCAGCGCGTTCAACGAGTCGTTg                                                                       <  2:241740/136‑1 (MQ=255)
                                                   tggccACGGCCGCTCGGCGCGGGGGCGGCAGGTGCGGGCGCCGCGACCAAAGGCTTGGCCGGTGCCGGTGTCGGCGCCGCCACCTTGACCGCTTCGGGCTTGCCCGCAGTCAGCGCGTTCAACGAGTCGTTGGCaa                                                                   <  1:108966/136‑1 (MQ=255)
                                                       cACGGCCGCTCGGCGCGGGGGCGGCAGGTGCGGGCGCCGCGACCAAAGGCTTGGCCGGTGCCGGTGTCGGGGCCGCCCCCATGCCAGCGTCGGGCGTGCCCGCCGGAGGCGCCTTCAACCGGTCGTTGGGAAAGGc                                                               >  2:168987/1‑136 (MQ=255)
                                                        aCGGCCGCTCGGCGCGGGGGCGGCAGGTGCGGGCGCCGCGACCAAAGGCTTGGCCGGTGCCGGTGTCGGCGCCGCCACCTTGACCGCTTCGGGCTTGCCCGCAGTCAGCGCGTTCAACGAGTCGTTGGCAAAGGcc                                                              <  2:284110/136‑1 (MQ=255)
                                                              gCTCGGCGCGGGGGCGGCAGGTGCGGGCGCCGCGACCAAAGGCTTGGCCGGTGCCGGTGTCGGCGCCGCCACCTTGACCGCTTCGGGCTTGCCCGCAGTCAGCGCGTTCAACGAGTCGTTGGCAAAGGCCGGGTTg                                                        <  1:129223/136‑1 (MQ=255)
                                                                  ggCGCGGGGGCGGCAGGTGCTGGCGCCGCGACCAAAGGCTTGGCCGGTGCCGGTGTGGGCGCCGCCCCCTTGACCGCGTCGGGCTTGCCCGCAGGCCGCGCGTTGAACGACTGGTTGGGAAAGGGCTGGTTGGGTc                                                    >  2:245835/1‑136 (MQ=255)
                                                                                      gggCGCCGCGACCAAAGGCTTGGCCGGTGCCGGTGTCGGCGCCGCCACCTTGACCGCTTCGGGCTTGCCCGCAGTCAGCGCGTTCAACGAGTCGTTGGCAAAGGCCGGGTTGGCTCGCGCCGGGCCGATGGCCATc                                <  1:39067/136‑1 (MQ=255)
                                                                                               gACCAAAGGCTTGGCCGGTGCCGGTGTGGGCGCCGCCCCCATGGCCGCGTCGGGGGTGCCCGCCGGGAGCGCGGTGAAAGAGGCGTTGGGAAAGGGCGGGGTGGGGGGCGCCGGGGCGGTGGCCCTCCGGcggggg                       >  2:398177/1‑131 (MQ=255)
                                                                                                aCCAAAGGCTTGGCCGGTGCCGGTGTCGGCGCCGCCACCATGACCGCTTCGGGCGTGCCCGCAGTCAGCGCGTTCAACGAGTAGGTGGGAAAGGCCCGGTTGGGTCGCGCCCGGCCGCAGGGCATCAGGCGGTCgg                      >  2:360536/1‑135 (MQ=255)
                                                                                                   aaaGGCTTGGCCGGTGCCGGTGTCGGCGCCGCCACCTTGACCGCTTCGGGCTTGCCCGCAGTCAGCGCGTTCAACGAGTCGTTGGCAAAGGCCGGGTTGGCTCGCGCCGGGCCGATGGCCATCAGGCTGTCGAGCt                   <  1:398177/136‑1 (MQ=255)
                                                                                                   aaaGGCTTGGCCGGTGCCGGTGTCGGCGCCGCCACCTTGACCGCTTCGGGCTTGCCCGCAGTCAGCGCGTTCAACGAGTCGTTGGCAAAGGCCGGGTTGGCTCGCGCCGGGCCGATGGCCATCAGGCTGTCGAGCt                   <  1:322707/136‑1 (MQ=255)
                                                                                                   aaaGGCTTGGCCGGTGCCGGTGTCGGCGCCGCCACCTTGACCGCTTCGGGCTTGCCCGCAGTCAGCGCGTTCAACGAGTCGTTGGCAAAGGCCGGGTTGGCTCGCGCCGGGCCGATGGCCATCAGGCTGTCGAGCt                   <  1:253813/136‑1 (MQ=255)
                                                                                                   aaaGGCTTGGCCGGTGCCGGTGTCGGCGCCGCCACCTTGACCGCTTCGGGCTTGCCCGCAGTCAGCGCGTTCAACGAGTCGTTGGCAAAGGCCGGGTTGGCTCGCGCCGGGCCGATGGCCATCAGGCTGTCGAGCt                   <  1:196717/136‑1 (MQ=255)
                                                                                                       gCTTGGCCGGTGCCGGTGTCGGCGCCGCCCCCATGACCGCGTCGGGCTTGGCCGCCGTCAGCGCGGTGAACGAGTGGTTGGGAAAGGCCGGGTTGGGTCGCGCCGGGCCGCTGGGCCTCAGGCGGTGGAGCTTTcc               >  2:168420/1‑136 (MQ=255)
                                                                                                                  gccggtgTCGGCGCCGCCACCTTGACCGCTTCGGGCTTGCCCGCAGTCAGCGCGTTCAACGAGTCGTTGGCAAAGGCCGGGTTGGCTCGCGCCGGGCCGATGGCCATCAGGCTGTCGAGCTTACCGATCTTGGTCa    <  1:296590/136‑1 (MQ=255)
                                                                                                                  gccggtgTCGGCGCCGCCACCTTGACCGCTTCGGGCTTGCCCGCAGTCAGCGCGTTCAACGAGTCGTTGGCAAAGGCCGGGTTGGCTCGCGCCGGGCCGATGGCCATCAGGCTGTCGAGCTTACCGATCTTGGTCa    <  1:276502/136‑1 (MQ=255)
                                                                                                                  gccggtgTCGGCGCCGCCACCTTGACCGCTTCGGGCTTGCCCGCAGTCAGCGCGTTCAACGAGTCGTTGGCAAAGGCCGGGTTGGCTCGCGCCGGGCCGATGGCCATCAGGCTGTCGAGCTTACCGATCTTGGTCa    <  1:162420/136‑1 (MQ=255)
                                                                                                                   ccggtgTCGGCGCCGCCACCTTGACCGCTTCGGGCTTGCCCGCAGTCAGCGCGTTCAACGAGTCGTTGGCAAAGGCCGGGTTGGCTCGCGCCGGGCCGATGGCCATCAGGCTGTCGAGCTTACCGATCTTGGTCAg   <  2:261502/136‑1 (MQ=255)
                                                                                                                   ccggtgTCGGCGCCGCCACCTTGACCGCTTCGGGCTTGCCCGCAGTCAGCGCGTTCAACGAGTCGTTGGCAAAGGCCGGGTTGGCTCGCGCCGGGCCGATGGCCATCAGGCTGTCGAGCTTACCGATCTTGGGCAg   <  1:55197/136‑1 (MQ=255)
                                                                                                                    cggtgTCGGCGCCGCCCCCATGACCGCGTCGGGCTTGCCCGCCGTCAGCGCGTTGAACGAGTCGTTGGCAAAGGCCGGGTTGGCTCGCGCCGGGCCGCTGGGCATCAGGCTGTCGAGCTTTCCGGTCTTGGTGAgg  >  2:388225/1‑136 (MQ=255)
                                                                                                                    cggtgTCGGCGCCGCCCCCATGACCGCATGGGGCGTGCCCCCAGTCAGCGCGGTGAACGAGTCGTTGGCAAAGGCCCGGTTGGGTCGCGCCCGGGCGCTGGGCCTCCGGGTGGGGGGGTGACCGAACTTGGTCAgg  >  2:213850/1‑136 (MQ=255)
                                                                                                                                    |                                                                                                                       
AACGCCTTGATCACACATGGCTGCATGCCACTGGCCAGGCGCAGCTGGCCCTGGCCACGGCCGCTCGGCGCGGGGGCGGCAGGTGCGGGCGCCGCGACCAAAGGCTTGGCCGGTGCCGGTGTCGGCGCCGCCACCTTGACCGCTTCGGGCTTGCCCGCAGTCAGCGCGTTCAACGAGTCGTTGGCAAAGGCCGGGTTGGCTCGCGCCGGGCCGATGGCCATCAGGCTGTCGAGCTTACCGATCTTGGTCAGG  >  NC_002947/6072006‑6072257

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑