| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,823,901:1 | +T | 100% | coding (1248/1293 nt) | kguT ← | 2‑ketogluconate transporter, putative |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,823,901 | 1 | . | T | 92.9% | 37.3 / ‑2.4 | 14 | coding (1248/1293 nt) | kguT | 2‑ketogluconate transporter, putative |
| Reads supporting (aligned to +/- strand): ref base . (0/1); new base T (6/7); total (6/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.39e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CACCTCGACGCGATCTTGCAGGCGTGCCATCAAGTCGTCGGACAGGCGTTTATACAGGACGATTCGCTTCTTCATTGCAGGTTTCCTAATGGTTCATGGCCAGACGCGAGCTATCGCGCTTTGCCCGGCC‑A‑GAGGTCTGGGAAGTATTGAGCGCAGCGGTAAGCGCCACTGCAGTCAGCAGCGCACCACACATGAACAGGTAAGAGGCGCCGGGCCCGCCCGTAGCCCCATTGAGGTAGCCCACCAGCCACGAGCCGCC > NC_002947/3823771‑3824029 | cACCTCGACGCGATCTTGCAGGCGTGCCATCAAGTCGTCGGACAGGCGTTTATACAGGACGATTCGCTTCTTCATTGCAGGTTTCCTAATGGTTCATGGCCAGACGCGAGCTATCGCGCTTTGCCCGGCC‑atgagg > 1:274699/1‑131 (MQ=255) cTCGACGCGATCTTGCAGGCGTGCCATCAAGTCGTCGGACAGGCGTTTATACAGGACGATTCGCTTCTTCATTGCAGGTTTCCTAATGGTTCATGGCCAGACGCGAGCTATCGCGCTTTGCCCGGCC‑ATGAGGTCt < 1:119606/136‑1 (MQ=255) gACGCGATCTTGCAGGCGTGCCATCAAGTCGTCGGACAGGCGTTTATACAGGACGATTCGCTTCTTCATTGCAGGTTTCCTAATGGTTCATGGCCAGACGCGAGCTATCGCGCTTTGCCCGGCC‑ATGAGGTCTggg > 2:254360/1‑136 (MQ=255) gACGCGATCTTGCAGGCGTGCCATCAAGTCGTCGGACAGGCGTTTATACAGGACGAATCGCTTCTTCATTGCAGGTTTCCTAATGGTTCATGGCCAGACGCGAGCTATCGCGCTTTGCCCGGCC‑ATGAGGTCTggg > 2:266365/1‑136 (MQ=255) cgcgATCTTGCAGGCGTGCCATCAAGTCGTCGGACAGGCGTTTATACAGGACGATTCGCTTCTTCATTGCAGGTTTCCTAATGGTTCATGGCCAGACGCGAGCTATCGCACTTTGCCCGGCC‑ATGAGGTCTGGGaa > 2:100519/1‑136 (MQ=255) aaaGTCGTCGGACTGGCGTTTATACCGGACTATTCGCTTCTTCATTGCAGGTTTCCTAATGGTTCATGCCCAGACGCTAGCTATCGCGCTTTGCCCGGCCTT‑GTGGTGTGGGAAGTTTTGCGCCCAGCGTTAAgcg < 2:259857/135‑1 (MQ=255) gACAGGCGTTTATACAGGACGATTCGCTTCTTCATTGCAGGTTTCCTAATGGTTCATGGCCAGACGCGAGCTATCGCGCTTTGCCCGGCC‑ATGAGGTCTGGGAAGTATTGAGCGCAGCGGTAAGCGCCACTGCAGt > 1:115943/1‑136 (MQ=255) aCGATTCGCTTCTTCATTGCAGGTTTCCTAATGGTTCATGGCCAGACGCTAGCTATCGCGCTTTGCCCGGCC‑ATGAGGTCTGGGAAGTATTGAGCGCAGCGGTAAGCGCCACTGCAGTCAGCAGCGCACCACACAt < 2:274699/136‑1 (MQ=255) aGGTTTCCTAATGGTTCATGGCCAGACGCGAGCTATCGCGCTTTGCCCGGCC‑ATGAGGTCTGGTAAGTATTGAGCGCAGCGGTAAGCGCCACTGCAGTCAGCAGCGCACCACACATGAACAGGTAAGAGGCGCCgg < 1:266365/136‑1 (MQ=255) aGGTTTCCTAATGGTTCATGGCCAGACGCGAGCTATCGCGCTTTGCCCGGCC‑ATGAGGTCTGGGAAGTATTGAGCGCAGCGGTAAGCGCCACTGCAGTCAGCAGCGCACCACACATGAACAGGTAAGAGGCGCCgg < 1:254360/136‑1 (MQ=255) aTGGCCAGACGCGAGCTATCGCGCTTTGCCCGGCC‑ATGAGGTCTGGGAAGTATTGAGCGCAGCGGTAAGCGCCACTGCAGTCAGCAGCGCACCACACATGAACAGGTAAGAGGCGCCGGGCCCGCCCGTAGCCCCa > 2:174236/1‑136 (MQ=255) ggCCAGACGCGAGCTATCGCGCTTTGCCCGGCC‑ATGAGGTCTGGGAAGTATTGAGCGCAGCGGTAAGCGCCACTGCAGTCAGCAGCGCACCACACATGAACAGGTAAGAGGCGCCGGGCCCGCCCGTAGCCCCAtt > 2:9439/1‑136 (MQ=255) cgAGCTATCGCGCTTTGCCCGGCC‑ATGAGGTCTGGGAAGTATTGAGCGCAGCGGTAAGCGCCACTGCAGTCAGCAGCGCACCACACATGAACAGGTAAGAGGCGCCGGGCCCGCCCGTAGCCCCATTGAGGTAGcc < 2:279061/136‑1 (MQ=255) gcgcTTTGCCCGGCC‑ATGAGGTCTGGGAAGTATTGAGCGCAGCGGTAAGCGCCACTGCAGTCAGCAGCGCACCACACATGAACAGGTAAGAGGCGCCGGGCCCGCCCGTAGCCCCATTGAGGTAGCCCACCAGCCa < 2:5089/136‑1 (MQ=255) ccGGCC‑ATGAGGTCTGGGAAGTATTGAGCGCAGCGGTAAGCGCCACTGCAGTCAGCAGCGCACCACACATGAACAGGTAAGAGGCGCCGGGCCCGCCCGTAGCCCCATTGAGGTAGCCCACCAGCCACGAgccgcc < 2:115943/136‑1 (MQ=255) | CACCTCGACGCGATCTTGCAGGCGTGCCATCAAGTCGTCGGACAGGCGTTTATACAGGACGATTCGCTTCTTCATTGCAGGTTTCCTAATGGTTCATGGCCAGACGCGAGCTATCGCGCTTTGCCCGGCC‑A‑GAGGTCTGGGAAGTATTGAGCGCAGCGGTAAGCGCCACTGCAGTCAGCAGCGCACCACACATGAACAGGTAAGAGGCGCCGGGCCCGCCCGTAGCCCCATTGAGGTAGCCCACCAGCCACGAGCCGCC > NC_002947/3823771‑3824029 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |