| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,753,153 | C→A | 36.4% | E899* (GAA→TAA) | PP_3316 ← | chaperone‑associated ATPase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,753,153 | 0 | C | A | 36.4% | 7.2 / 7.5 | 11 | E899* (GAA→TAA) | PP_3316 | chaperone‑associated ATPase |
| Reads supporting (aligned to +/- strand): ref base C (5/2); new base A (2/2); total (7/4) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.76e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.01e-01 | |||||||||||
CATTTTCACTGTCGAGCTCTTTGGCCGGCGCGGCTTGCCGGCGGTCGAACCCGACCCGCTCCGCCTTGTCGTCCCAGCGCACATGGGCGTGATCACCCTTGCCAATGCCGCCGCCGAGCATTTCACGTGCCAGTGCCGTCTCCAGCTCGCTGCGGATCAGTCGCTTGAGCTCGCGGGCACCGAATTCGGGCTTGTAGCCGACTTGCGCCAAGTGATCGACCAGTGTCTCGTCGAACGTCAGCGTC > NC_002947/3753030‑3753274 | cATTTTCACTGTCGAGCTCTTTGGCCGGCGCGGCTTGCCGGCGGTCGAACCCGACCCGCTCCGCCTTGTCGTCCCAGCGCACATGGGCGTGATCACCCTTGCCAATGCCGCCGCCGAGCATTTCACGTGCCAGTGc < 2:270849/136‑1 (MQ=255) ccGGCGCGGCTTGCCGGCGGTCGAACCCGACCCGCTCCGCCTTGTCGTCCCAGCGCACATGGGCGTGATCACCCTTGCCAATGCCGCCGCCGAGCATTTCACGTGCCAGTGCCGTCTCCAGCTCGCTGCGGATCAg > 1:259149/1‑136 (MQ=255) ccGGCGCGGCTTGCCGGCGGTCGAACCCGACCCGCTCCGCCTTGTCGTCCCAGCGCACATGGGCGTGATCACCCTTGCCAATGCCGCCGCCGAGCATTTCACGTGCCAGTGCCGTCTCCAGCTCGCTGCGGATCAg > 1:52853/1‑136 (MQ=255) cgGCTTGCCGGCGGTCGAACCCGACCCGCTCCGCCTTGTCGTCCCAGCGCACATGGGCGTGATCACCCTTGCCAATGCCGCCGCCGAGCATTTAACGTGCCAGTGCCGTCTCCAGCTCGCTGCGGATCAGTCGCtt > 1:262567/1‑136 (MQ=255) cgGCTTGCCGGCGGTCGAACCCGACCCGCTCCGCCTTGTCGTCCCAGCGCACATGGGCGTGATCACCCTTGCCAATGCCGCCGCCGAGCATTTAACGTGCCAGTGCCGTCTCCAGCTCGCTGCGGATCAGTCGCtt > 1:264052/1‑136 (MQ=255) gcACATGGGCGTGATCACCCTTGCCAATGCCGCCGCCGAGCATTTCACGTGCCAGTGCCGTCTCCAGCTCGCTGCGGATCAGTCGCTTGAGCTCGCGGGCACCGAATTCGGGCTTGTAGCCGACGTGCGCCAAGTg > 1:146314/1‑136 (MQ=255) gcACATGGGCGTGATCACCCTTGCCAATGCCGCCGCCGAGCATTTAACGTGCCAGTGCCGTCTCCAGCTCGCTGCGGATCAGTCGCTTGAGCTCGCGGGCACCGAATTCGGGCTTGTAGCCGACTTGCGCCAAGTg < 2:262567/136‑1 (MQ=255) gcACATGGGCGTGATCACCCTTGCCAATGCCGCCGCCGAGCATTTAACGTGCCAGTGCCGTCTCCAGCTCGCTGCGGATCAGTCGCTTGAGCTCGCGGGCACCGAATTCGGGCTTGTAGCCGACTTGCGCCAAGTg < 2:264052/136‑1 (MQ=255) tCACCCTTGCCAATGCCGCCGCCGAGCATTTCACGTGCCAGTGCCGTCTCCAGCTCGCTGCGGATCAGTCGCTTGAGCTCGCGGGCACCGAATTCGGGCTTGTAGCCGACTTGCGCCAAGTGATCGACCAGTGtct > 2:168841/1‑136 (MQ=255) gccgccgccGAGCATTTCACGTGCCAGTGCCGTCTCCAGCTCGCTGCGGATCAGTCGCTTGAGCTCGCGGGCACCGAATTCGGGCTTGTAGCCGACTTGCGCCAAGTGATCGACCAGTGTCTCGTCGAACGTCAGc < 1:228849/136‑1 (MQ=255) gccgccgAGCATTTCACGTGCCAGTGCCGTCTCCAGCTCGCTGCGGATCAGTCGCTTGAGCTCGCGGGCACCGAATTCGGGCTTGTAGCCCACTTGCGCCAAGTGATCGACCAGTGTCTCGTCGAACGTCAGCGTc > 1:116093/1‑136 (MQ=255) | CATTTTCACTGTCGAGCTCTTTGGCCGGCGCGGCTTGCCGGCGGTCGAACCCGACCCGCTCCGCCTTGTCGTCCCAGCGCACATGGGCGTGATCACCCTTGCCAATGCCGCCGCCGAGCATTTCACGTGCCAGTGCCGTCTCCAGCTCGCTGCGGATCAGTCGCTTGAGCTCGCGGGCACCGAATTCGGGCTTGTAGCCGACTTGCGCCAAGTGATCGACCAGTGTCTCGTCGAACGTCAGCGTC > NC_002947/3753030‑3753274 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |