| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,827,432 | G→A | 40.0% | S193S (AGC→AGT) | ptxS ← | 2‑ketogluconate utilization repressor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,827,432 | 0 | G | A | 40.0% | 1.5 / 6.4 | 10 | S193S (AGC→AGT) | ptxS | 2‑ketogluconate utilization repressor |
| Reads supporting (aligned to +/- strand): ref base G (3/3); new base A (2/2); total (5/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.67e-01 | |||||||||||
AACGAAGCCAGTTGGCCCTGCAGCCCGGCGCCGATTTCCAGTACCTGCTGACGCATGCCTGGCCGTCGGCTGATCGACGCGCCGAATGCCTGCACCCGCTCCAGGCGTGAACTGGTGCCATCGAGGGGTTCGCTGACCGCCAGGATGTCGCGGTAGCCCTGGGCTTGCAGGTGGTCCAGGGCCTGCTCGACTGCGTCAGCATTGTCCAG > NC_002947/3827301‑3827509 | acgccgttgaaggtgaagatggcttgcggaccatgaccactgtgggcaagaaacgaagccagttggccctgcagcccggcgccgaATGCCTGCACCCGCTCCAGGCGTGAACTGGTGCCATCGAGGGGTTCACTGa > 2:239372‑M2/86‑136 (MQ=255)acgccgttgaaggtgaagatggcttgcggaccatgaccactgtgggcaagaaacgaagccagttggccctgcagcccggcgccgaATGCCTGCACCCGCTCCAGGCGTGAACTGGTGCCATCGAGGGGTTCACTGa > 2:240775‑M2/86‑136 (MQ=255) gaccatgaccactgtgggcaagaaacgaagccagttggccctgcagcccggcgccgaATGCCTGCACCCGCTCCAGGCGTGAACTGGTGCCATCGAGGGGTTCGCTGACCGCCAGGATGTCGCGGTAGCCCTGGGc > 1:231302‑M2/58‑136 (MQ=255) cttgacctctgtgggcaagaaaggaagcgagttggccctgcagcccggcgccgaATGCCTGCACGCGCTCCTGGCGTGTACTGGTGCGATCGAGGGGTTCGCTGACCGCCAGGATGTCGCGGTAGCCCTGGGCTTg < 1:101523‑M2/82‑1 (MQ=255) ttgaccactgtgggcaagaaacgaagccagttggccctgcagcccggcgccgaATGGCTGCACCCGCTCCAGGCGTGAACTGGTGCCATCGAGGGGTTCGCTGACCGCCAGGATGTCGCGGTAGCCCTGGGCTTGc < 2:140012‑M2/83‑1 (MQ=255) tgaccactgtgggcaagaaacgaagccagttggccctgcagcccggcgccgaATGCCTGCACCCGCTCCAGGCGTGAACTGGTGCCATCGAGGGGTTCGCTGACCGCCAGGATGTCGCGGTAGCCCTGGGCTTGCa < 2:217561‑M2/84‑1 (MQ=255) gccagttggccctgcagcccggcgccgaATGCCTGCACCCGCTCCAGGCGTGAACTGGTGCCATCGAGGGGTTCACTGACCGCCAGGATGTCGCGGTAGCCCTGGGCTTGCAGGTGGTCCAGGGCCTGCTCGACTg < 1:240775‑M2/108‑1 (MQ=255) gccagttggccctgcagcccggcgccgaATGCCTGCACCCGCTCCACGCGCGAACTTGTGCCATCGAGGGGTTCACTGACCGCCTGGATGTCGCGGTAGCCCTGGGCTTGCAGGTGGTCCAGGGCCTGCTCGACTg < 1:239372‑M2/108‑1 (MQ=255) gcccggcgccgaATGCCTGCACCCGCTCCAGGCGTGAACTGGTGCCATCGAGGGGTTCGCTGACCGCCAGGATGTCGCGGTAGCCCTGGGCTTGCAGGTGGTCCAGGGCCTGCTCGACTGCGTCAGCATTGTCCAg > 1:49973‑M2/13‑136 (MQ=255) gcccggcgccgaATGCCTGCACCCGCTCCAGGCGTGAACTGGTGCCATCGAGGGGTTCGCTGACCGCCAGGAAGTCGCGGTAGCCCTGGGCTTGCAGGTGGTCGAGGGCCTGCTCGACTGCGGCAGCATTGTCCAg > 1:43204‑M2/13‑136 (MQ=255) | AACGAAGCCAGTTGGCCCTGCAGCCCGGCGCCGATTTCCAGTACCTGCTGACGCATGCCTGGCCGTCGGCTGATCGACGCGCCGAATGCCTGCACCCGCTCCAGGCGTGAACTGGTGCCATCGAGGGGTTCGCTGACCGCCAGGATGTCGCGGTAGCCCTGGGCTTGCAGGTGGTCCAGGGCCTGCTCGACTGCGTCAGCATTGTCCAG > NC_002947/3827301‑3827509 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |