| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,425,830 | T→C | 36.4% | C33R (TGC→CGC) | PP_3915 → | hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,425,830 | 0 | T | C | 36.4% | 3.3 / 7.1 | 11 | C33R (TGC→CGC) | PP_3915 | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (5/2); new base C (2/2); total (7/4) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.76e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCCTCGCCGGCGGCATGGATTGATGAGAGGTATCAGCTGTGAGTCGGAAGAAAAGCCACTTCACCATTGTTTCCAGCACAGAGCTGGAGGAATTGCGCCGCGACCGTGAGCGACTGAACGCCCTGGAGTCATGCTGCTGGGATGTTCGTTTCGAGAGCCATTCGAATGGGATGGATGGCGACTACACGATCGGCATTGAGATCGTCGGCCACTACATGGGCAAACCATGTGCGCGGGTGCTCGGCGAGAACTACAACGAGAACCTGCG > NC_002947/4425696‑4425963 | gCCTCGCCGGCGGCATGGATTGATGAGAGGTATCAGCTGTGAGTCGGAAGAAAAGCCACTTCACCATTGTTTCCAGCACAGAGCTGGAGGAATTGCGCCGCGCCCGTGAGCGACTGAACGCCCTGGAGTCAtgctg > 1:204840/1‑136 (MQ=255) gaaAAGCCACTTCACCATTGTTTCCAGCACAGAGCTGGAGGAATTGCGCCGCGACCGTGAGCGACTGAACGCCCTGGAGTCATGCTGCTGGGATGTTCGTTTCGAGAGCCATTCGAATGGGATGGATGGCGACTac > 1:227741/1‑136 (MQ=255) aCCATTGTTTCCAGCACAGAGCTGGAGGAATTGCGCCGCGACCGTGAGCGACTGAACGCCCTGGAGTCATGCTGCTGGGATGTTCGTTTCGAGAGc > 1:53941/1‑96 (MQ=255) aCCATTGTTTCCAGCACAGAGCTGGAGGAATTGCGCCGCGACCGTGAGCGACTGAACGCCCTGGAGTCATGCTGCTGGGATGTTCGTTTCGAGAGc < 2:53941/96‑1 (MQ=255) aCCATTGTTTCCAGCACAGAGCTGGAGGAATTGCGCCGCGACCGTGAGCGACTGAACGCCCTGGAGTCATGCCGCTGGGATGTTCGTTTCGAGAGc > 1:284346/1‑96 (MQ=255) aCCATTGTTTCCAGCACAGAGCTGGAGGAATTGCGCCGCGACCGTGAGCGACTGAACGCCCTGGAGTCATGCCGCTGGGATGTTCGTTTCGAGAGc > 1:285736/1‑96 (MQ=255) aCCATTGTTTCCAGCACAGAGCTGGAGGAATTGCGCCGCGACCGTGAGCGACTGAACGCCCTGGAGTCATGCCGCTGGGATGTTCGTTTCGAGAGc < 2:284346/96‑1 (MQ=255) aCCATTGTTTCCAGCACAGAGCTGGAGGAATTGCGCCGCGACCGTGAGCGACTGAACGCCCTGGAGTCATGCCGCTGGGATGTTCGTTTCGAGAGc < 2:285736/96‑1 (MQ=255) tGGAGGAATTGCGCCGCGACCGTGAGCGACTGAACGCCCTGGAGTCATGCTGCTGGGATGTTCGTTTCGAGAGCCATTCGAATGGGATGGATGGCGACTACACGATCGGCATTGAGATCGTCGGCCACTACATggg > 2:295213/1‑136 (MQ=255) gcgcCGCGACCGTGAGCGACTGAACGCCCTGGAGTCATGCTGCTGGGATGTTCGTTTCGAGAGCCATTCGAATGGGATGGATGGCGACTACGCGATCGGCATTGAGATCGTCGGCCACTACATGGGCAAACCAtgt < 1:59706/136‑1 (MQ=255) gcgcCGCGACCGTGAGCGACTGAACGCCCTGGAGTCATGCTGCTGGGATGTTCGTTTCGAGAGCCATTCGAATGGGATGGATGGCGACTACACGATCGGCATTGAGATCGTCGGCCACTACATGGGCAATCCAtgt > 1:83787/1‑136 (MQ=255) ggAGTCATGCTGCTGGGATGTTCGTTTCGAGAGCCATTCGAATGGGATGGATGGCGACTACACGATCGGCATTGAGATCGTCGGCCACTACATGGGCAAACCATGTGCGCGGGTGCTCGGCGAGAACTACAACgag > 2:50087/1‑136 (MQ=255) tgctgGGATGTTCGTTTCGAGAGCCATTCGAATGGGATGGATGGCGACTACACGATCGGCATTGAGATCGTCGGCCACTACATGGGCAAACCATGTGCGCGGGTGCTCGGCGAGAACTACAACGAGAACCTgcg < 1:108249/134‑1 (MQ=255) tgctgGGATGTTCGTTTCGAGAGCCATTCGAATGGGATGGATGGCGACTACACGATCGGCATTGAGATCGTCGGCCACTACATGGGCAAACCATGTGCGCGGGTGCTCGGCGAGAACTACAACGAGAACCTgcg > 2:108249/1‑134 (MQ=255) | GCCTCGCCGGCGGCATGGATTGATGAGAGGTATCAGCTGTGAGTCGGAAGAAAAGCCACTTCACCATTGTTTCCAGCACAGAGCTGGAGGAATTGCGCCGCGACCGTGAGCGACTGAACGCCCTGGAGTCATGCTGCTGGGATGTTCGTTTCGAGAGCCATTCGAATGGGATGGATGGCGACTACACGATCGGCATTGAGATCGTCGGCCACTACATGGGCAAACCATGTGCGCGGGTGCTCGGCGAGAACTACAACGAGAACCTGCG > NC_002947/4425696‑4425963 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |