Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,992,127	T→A	28.6%	A325A (GCA→GCT)	PP_4399 ←	glutamine synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,992,127	0	T	A	28.6%	18.0 / 5.7	14	A325A (GCA→GCT)	PP_4399	glutamine synthetase
Reads supporting (aligned to +/- strand):  ref base T (4/6);  new base A (2/2);  total (6/8)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGAGCCTTTCAAGGGCCGCGTGCAGCGTGCACGGCAGGCTCAGGTGCTCGGGCACTTCGAATTCGCCCTGGATGGCCGGCGATGGCTGCAAGCGCTGCTCGATGCCATGCAGCCCGGCGGCCAGGCTGGCTGCAATGGCCAGGTAGGGGTTGGCATCGGCGCCAGGCAGGCGGTTTTCCACCCGTCGCGCGACCGGCGCACTGGCCGGAATGCGCAAGCCAGCGGCGCGATTGTCCTC  >  NC_002947/4991997‑4992234                                                                                                                                   |                                                                                                            tGAGCCTTTCAAGGGCCGCGTGCAGCGTGCACGGCAGGCTCAGGTGCTCGGGCACTTCGAATTCGCCCTGGATGGCCGGCGATGGCTGCAAGCGCTGCTCGATGCCATGCAGCCCGGCGGCCAGGCTGGCTGCAAt                                                                                                        <  1:41915/136‑1 (MQ=255)                 cgcgTGCAGCGTGCACGGCAGGCTCAGGTGCTCGGGCACTTCGAATTCGCCCTGGATGGCCGGCGATGGCTGCAAGCGCTGCTCGATGCCATGCAGCCCGGCGGCCAGGCTGGCTGCAATGGCCAGGTAGGGGTTg                                                                                        <  1:110459/136‑1 (MQ=255)                  gcgtgcagcgtgcaCGGCAGGCTCAGGTGCTCGGGCACTTCGAATTCGCCCTGGATGGCCGGCGATGGCTGCAAGCGCTGCTCGATGCCATGCAGCCCGGCGGCCAGGCTGGCAGCGATGGCCAGGTACGGGTTgg                                                                                       >  2:152265/1‑136 (MQ=255)                  gcgtgcagcgtgcaCGGCAGGCTCAGGTGCTCGGGCACTTCGAATTCGCCCTGGATGGCCGGCGATGGCTGCAAGCGCTGCTCGATGCCATGCAGCCCGGCGGCCAGGCTGGCAGCGATGGCCAGGTACGGGTTgg                                                                                       >  2:153770/1‑136 (MQ=255)                    gtgcagcgtgcaCGGCAGGCTCAGGTGCTCGGGCACTTCGAATTCGCCCTGGATGGCCGGCGATGGCTGCAAGCGCTGCTCGATGCCATGCAGCCCGGCGGCCAGGCTGGCTGCAATGGCCAGGTAGGGGTTGGCa                                                                                     >  1:17610/1‑136 (MQ=255)                      gcagcgtgcaCGGCAGGCTCAGGTGCTCGGGCACTTCGAATTCGCCCTGGATGGCCGGCGATGGCTGCAAGCGCTGCTCGATGCCATGCAGCCCGGCGGCCAGGCTGGCTGCAATGGCCAGGTAGGGGTTGGCATc                                                                                   >  2:290168/1‑136 (MQ=255)                             gcaCGGCAGGCTCAGGTGCTCGGGCACTTCGAATTCGCCCTGGATGGCCGGCGATGGCTGCAAGCGCTGCTCGATGCCATGCAGCCCGGCGGCCAGGCTGGCAGCGATGGCCAGGTACGGGTTGGCATCGGCGccc                                                                            <  1:152265/136‑2 (MQ=255)                             gcaCGGCAGGCTCAGGTGCTCGGGCACTTCGAATTCGCCCTGGATGGCCGGCGATGGCTGCAAGCGCTGCTCGATGCCATGCAGCCCGGCGGCCAGGCTGGCAGCGATGGCCAGGTACGGGTTGGCATCGGCGccc                                                                            <  1:153770/136‑2 (MQ=255)                                                    gCACTTCGAATTCGCCCTGGATGGCCGGCGATGGCTGCAAGCGCTGCTCGATGCCATGCAGCCCGGCGGCCAGGCTGGCTGCAATGGCCAGGTAGGGGTTGGCATCGGCGCCAGGCAGGCGGTTTTCCACCCGTcg                                                     >  1:176776/1‑136 (MQ=255)                                                     cACTTCGAATTCGCCCTGGATGGCCGGCGATGGCTGCAAGCGCTGCTCGATGCCATGCAGCCCGGCGGCCAGGCTGGCTGCAATGGCCAGGTAGGGGTTGGCATCGGCGCCAGGCAGGCGGTTTTCCACCCGTcgc                                                    >  1:535/1‑136 (MQ=255)                                                              ttCGCCCTGGATGGCCGGCGATGGCTGCAAGCGCTGCTCGATGCCATGCAGCCCGGCGGCCAGGCTGGCTGCAATGGCCAGGTAGGGGTTGGCATCGGCGCCAGGCAGGCGGTTTTCCACCCGTCGCGCGACCGgc                                           <  1:222126/136‑1 (MQ=255)                                                              ttCGCCCTGGATGGCCGGCGATGGCTGCAAGCGCTGCTCGATGCCATGCAGCCCGGCGGCCAGGCTGGCTGCAATGGCCAGGTAGGGGTTGGCATCGGCGCCAGGCAGGCGGTTTTCCACCCGTCGCGCGACCGgc                                           <  1:53317/136‑1 (MQ=255)                                                                          ggCCGGCGATGGCTGCAAGCGCTGCTCGATGCCATGCAGCCCGGCGGCCAGGCTGGCTGCAATGGCCAGGTAGGGGTTGGCATCGGCGCCAGGCAGGCGGTTTTCCACCCGTCGCGCGACCGGCGCACTGGCCGGa                               <  2:17610/136‑1 (MQ=255)                                                                                                       tGCCATGCAGCCCGGCGGCCAGGCTGGCTGCAATGGCCAGGTAGGGGTTGGCATCGGCGCCAGGCAGGCGGTTTTCCACCCGTCGCGCGACCGGCGCACTGGCCGGAATGCGCAAGCCAGCGGCGCGATTGTCCTc  <  2:195600/136‑1 (MQ=255)                                                                                                                                   |                                                                                                            TGAGCCTTTCAAGGGCCGCGTGCAGCGTGCACGGCAGGCTCAGGTGCTCGGGCACTTCGAATTCGCCCTGGATGGCCGGCGATGGCTGCAAGCGCTGCTCGATGCCATGCAGCCCGGCGGCCAGGCTGGCTGCAATGGCCAGGTAGGGGTTGGCATCGGCGCCAGGCAGGCGGTTTTCCACCCGTCGCGCGACCGGCGCACTGGCCGGAATGCGCAAGCCAGCGGCGCGATTGTCCTC  >  NC_002947/4991997‑4992234

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑