| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,992,130 | A→G | 28.6% | I324I (ATT→ATC) | PP_4399 ← | glutamine synthetase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,992,130 | 0 | A | G | 28.6% | 14.7 / 6.4 | 14 | I324I (ATT→ATC) | PP_4399 | glutamine synthetase |
| Reads supporting (aligned to +/- strand): ref base A (4/6); new base G (2/2); total (6/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.44e-01 | |||||||||||
TGAGCCTTTCAAGGGCCGCGTGCAGCGTGCACGGCAGGCTCAGGTGCTCGGGCACTTCGAATTCGCCCTGGATGGCCGGCGATGGCTGCAAGCGCTGCTCGATGCCATGCAGCCCGGCGGCCAGGCTGGCTGCAATGGCCAGGTAGGGGTTGGCATCGGCGCCAGGCAGGCGGTTTTCCACCCGTCGCGCGACCGGCGCACTGGCCGGAATGCGCAAGCCAGCGGCGCGATTGTCCTCGGACCAGCACGCATTGTTCGGCGAGGCATAG > NC_002947/4991997‑4992265 | tGAGCCTTTCAAGGGCCGCGTGCAGCGTGCACGGCAGGCTCAGGTGCTCGGGCACTTCGAATTCGCCCTGGATGGCCGGCGATGGCTGCAAGCGCTGCTCGATGCCATGCAGCCCGGCGGCCAGGCTGGCTGCAAt < 1:41915/136‑1 (MQ=255) cgcgTGCAGCGTGCACGGCAGGCTCAGGTGCTCGGGCACTTCGAATTCGCCCTGGATGGCCGGCGATGGCTGCAAGCGCTGCTCGATGCCATGCAGCCCGGCGGCCAGGCTGGCTGCAATGGCCAGGTAGGGGTTg < 1:110459/136‑1 (MQ=255) gcgtgcagcgtgcaCGGCAGGCTCAGGTGCTCGGGCACTTCGAATTCGCCCTGGATGGCCGGCGATGGCTGCAAGCGCTGCTCGATGCCATGCAGCCCGGCGGCCAGGCTGGCAGCGATGGCCAGGTACGGGTTgg > 2:152265/1‑136 (MQ=255) gcgtgcagcgtgcaCGGCAGGCTCAGGTGCTCGGGCACTTCGAATTCGCCCTGGATGGCCGGCGATGGCTGCAAGCGCTGCTCGATGCCATGCAGCCCGGCGGCCAGGCTGGCAGCGATGGCCAGGTACGGGTTgg > 2:153770/1‑136 (MQ=255) gtgcagcgtgcaCGGCAGGCTCAGGTGCTCGGGCACTTCGAATTCGCCCTGGATGGCCGGCGATGGCTGCAAGCGCTGCTCGATGCCATGCAGCCCGGCGGCCAGGCTGGCTGCAATGGCCAGGTAGGGGTTGGCa > 1:17610/1‑136 (MQ=255) gcagcgtgcaCGGCAGGCTCAGGTGCTCGGGCACTTCGAATTCGCCCTGGATGGCCGGCGATGGCTGCAAGCGCTGCTCGATGCCATGCAGCCCGGCGGCCAGGCTGGCTGCAATGGCCAGGTAGGGGTTGGCATc > 2:290168/1‑136 (MQ=255) gcaCGGCAGGCTCAGGTGCTCGGGCACTTCGAATTCGCCCTGGATGGCCGGCGATGGCTGCAAGCGCTGCTCGATGCCATGCAGCCCGGCGGCCAGGCTGGCAGCGATGGCCAGGTACGGGTTGGCATCGGCGccc < 1:152265/136‑2 (MQ=255) gcaCGGCAGGCTCAGGTGCTCGGGCACTTCGAATTCGCCCTGGATGGCCGGCGATGGCTGCAAGCGCTGCTCGATGCCATGCAGCCCGGCGGCCAGGCTGGCAGCGATGGCCAGGTACGGGTTGGCATCGGCGccc < 1:153770/136‑2 (MQ=255) gCACTTCGAATTCGCCCTGGATGGCCGGCGATGGCTGCAAGCGCTGCTCGATGCCATGCAGCCCGGCGGCCAGGCTGGCTGCAATGGCCAGGTAGGGGTTGGCATCGGCGCCAGGCAGGCGGTTTTCCACCCGTcg > 1:176776/1‑136 (MQ=255) cACTTCGAATTCGCCCTGGATGGCCGGCGATGGCTGCAAGCGCTGCTCGATGCCATGCAGCCCGGCGGCCAGGCTGGCTGCAATGGCCAGGTAGGGGTTGGCATCGGCGCCAGGCAGGCGGTTTTCCACCCGTcgc > 1:535/1‑136 (MQ=255) ttCGCCCTGGATGGCCGGCGATGGCTGCAAGCGCTGCTCGATGCCATGCAGCCCGGCGGCCAGGCTGGCTGCAATGGCCAGGTAGGGGTTGGCATCGGCGCCAGGCAGGCGGTTTTCCACCCGTCGCGCGACCGgc < 1:222126/136‑1 (MQ=255) ttCGCCCTGGATGGCCGGCGATGGCTGCAAGCGCTGCTCGATGCCATGCAGCCCGGCGGCCAGGCTGGCTGCAATGGCCAGGTAGGGGTTGGCATCGGCGCCAGGCAGGCGGTTTTCCACCCGTCGCGCGACCGgc < 1:53317/136‑1 (MQ=255) ggCCGGCGATGGCTGCAAGCGCTGCTCGATGCCATGCAGCCCGGCGGCCAGGCTGGCTGCAATGGCCAGGTAGGGGTTGGCATCGGCGCCAGGCAGGCGGTTTTCCACCCGTCGCGCGACCGGCGCACTGGCCGGa < 2:17610/136‑1 (MQ=255) tGCCATGCAGCCCGGCGGCCAGGCTGGCTGCAATGGCCAGGTAGGGGTTGGCATCGGCGCCAGGCAGGCGGTTTTCCACCCGTCGCGCGACCGGCGCACTGGCCGGAATGCGCAAGCCAGCGGCGCGATTGTCCTc < 2:195600/136‑1 (MQ=255) aaTGGCCAGGTAGGGGTTGGCATCGGCGCCAGGCAGGCGGTTTTCCACCCGTCGCGCGACCGGCGCACTGGCCGGAATGCGCAAGCCAGCGGCGCGATTGTCCTCGGACCAGCACGCATTGTTCGGCGAGGCATAg > 1:132986/1‑136 (MQ=255) | TGAGCCTTTCAAGGGCCGCGTGCAGCGTGCACGGCAGGCTCAGGTGCTCGGGCACTTCGAATTCGCCCTGGATGGCCGGCGATGGCTGCAAGCGCTGCTCGATGCCATGCAGCCCGGCGGCCAGGCTGGCTGCAATGGCCAGGTAGGGGTTGGCATCGGCGCCAGGCAGGCGGTTTTCCACCCGTCGCGCGACCGGCGCACTGGCCGGAATGCGCAAGCCAGCGGCGCGATTGTCCTCGGACCAGCACGCATTGTTCGGCGAGGCATAG > NC_002947/4991997‑4992265 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |