| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,728,186 | C→T | 22.2% | C253Y (TGC→TAC) | opgH ← | glucans biosynthesis glucosyltransferase H |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,728,186 | 0 | C | T | 22.2% | 29.0 / 5.1 | 18 | C253Y (TGC→TAC) | opgH | glucans biosynthesis glucosyltransferase H |
| Reads supporting (aligned to +/- strand): ref base C (5/9); new base T (2/2); total (7/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.11e-01 | |||||||||||
TGCTCGGCCACGGCAATGTCGGTGTCGTTGGTGTCGCTGAGCACAAAGAAGTCGAACCGGTCGAGGTTGCCGCTGGCGGCCACCGACTCGAACGTTGCGCGCAGGCCGGCGAACACCCGCGGCACGTCTTCGTTGCAGATCGGCATCACCAGCGCGGTACGCGCCTCGGGCGCGATCGGCTCGTTGCCTGCGCTGCTACCGGAAATCTTGTATTTGTCACGCCCGGTGAGCAGCTCGAGGAAGCCCATCA > NC_002947/5728051‑5728300 | tGCTCGGCCACGGCAATGTCGGTGTCGTTGGTGTCGCTGAGCACAAAGAAGTCGAACCGGTCGAGGTTGCCGCTGGCGGCCACCGACTCGAACGTTGCGCGCAGGCCGGCGAACACCCGCGGCACGTCTTCGTTGc < 2:173058/136‑1 (MQ=255)tGCTCGGCCACGGCAATGTCGGTGTCGTTGGTGTCGCTGAGCACAAAGAAGTCGAACCGGTCGAGGTTGCCGCTGGCGGCCACCGACTCGAACGTTGCGCGCAGGCCGGCGAACACCCGCGGCACGTCTTCGTTGc < 2:268779/136‑1 (MQ=255) cTCGGCCACGGCAATGTCGGTGTCGTTGGTGTCGCTGAGCACAAAGAAGTCGAACCGGTCGAGGTTGCCGCTGGCGGCCACCGACTCGAACGTTGCGCGCAGGCCGGCGAACACCCGCGGCACGTCTTCGTTGTAg > 1:10192/1‑136 (MQ=255) cTCGGCCACGGCAATGTCGGTGTCGTTGGTGTCGCTGAGCACAAAGAAGTCGAACCGGTCGAGGTTGCCGCTGGCGGCCACCGACTCGAACGTTGCGCGCAGGCCGGCGAACACCCGCGGCACGTCTTCGTTGTAg > 1:234604/1‑136 (MQ=255) cTCGGCCACGGCAATGTCGGTGTCGTTGGTGTCGCTGAGCACAAAGAAGTCGAACCGGTCGAGGTTGCCGCTGGCGGCCACCGACTCGAACGTTGCGCGCAGGCCGGCGAACACCCGCGGCACGTCTTCGTTGCAg > 1:141519/1‑136 (MQ=255) cTCGGCCACGGCAATGTCGGTGTCGTTGGTGTCGCTGAGCACAAAGAAGTCGAACCGGTCGAGGTTGCCGCTGGCGGCCACCGACTCGAACGTTGCGCGCAGGCCGGCGAACACCCGCGGCACGTCTTCGTTGCAg > 1:175690/1‑136 (MQ=255) ccACGGCAATGTCGGTGTCGTTGGTGTCGCTGAGCACAAAGAAGTCGAACCGGTCGAGGTTGCCGCTGGCGGCCACCGACTCGAACGTTGCGCGCAGGCCGGCGAACACCCGCGGCACGTCTTCGTTGCAGATCgg > 2:177930/1‑136 (MQ=255) ggCAATGTCGGTGTCGTTGGTGTCGCTGAGCACAAAGAAGTCGAACCGGTCGAGGTTGCCGCTGGCGGCCACCGACTCGAACGTTGCGCGCAGGCCGGCGAACACCCGCGGCACGTCTTCGTTGCAGATCGGCATc < 1:79880/136‑1 (MQ=255) aTGTCGGTGTCGTTGGTGTCGCTGAGCACAAAGAAGTCGAACCGGTCGAGGTTGCCGCTGGCGGCCACCGACTCGAACGTTGCGCGCAGGCCGGCGAACACCCGCGGCACGTCTTCGTTGCAGATCGGCATCACCa < 2:206928/136‑1 (MQ=255) ggTGTCGTTGGTGTCGCTGAGCACAAAGAAGTCGAACCGGTCGAGGTTGCCGCTGGCGGCCACCGACTCGAACGTTGCGCGCAGGCCGGCGAACACCCGCGGCACGTCTTCGTTGCAGATCGGCATCACCAGcgcg < 1:129241/136‑1 (MQ=255) tgtCGTTGGTGTCGCTGAGCACAAAGAAGTCGAACCGGTCGAGGTTGCCGCTGGCGGCCACCGACTCGAACGTTGCGCGCAGGCCGGCGAACACCCGCGGCACGTCTTCGTTGTAGATCGGCATCACCAGCGCGGt < 2:234604/136‑1 (MQ=255) tgtCGTTGGTGTCGCTGAGCACAAAGAAGTCGAACCGGTCGAGGTTGCCGCTGGCGGCCACCGACTCGAACGTTGCGCGCAGGCCGGCGAACACCCGCGGCACGTCTTCGTTGTAGATCGGCATCACCAGCGCGGt < 2:10192/136‑1 (MQ=255) tgtCGTTGGTGTCGCTGAGCACAAAGAAGTCGAACCGGTCGAGGTTGCCGCTGGCGGCCACCGACTCGAACGTTGCGCGCAGGCCGGCGAACACCCGCGGCACGTCTTCGTTGCAGATCGGCATCACCAGCGCGGt < 2:175690/136‑1 (MQ=255) aaCCGGTCGAGGTTGCCGCTGGCGGCCACCGACTCGAACGTTGCGCGCAGGCCGGCGAACACCCGCGGCACGTCTTCGTTGCAGATCGGCATCACCAGCGCGGTACGCGCCTCGGGCGCGATCGGCTCGTTGCCTg > 1:60149/1‑136 (MQ=255) tGGCGGCCACCGACTCGAACGTTGCGCGCAGGCCGGCGAACACCCGCGGCACGTCTTCGTTGCAGATCGGCATCACCAGCGCGGTACGCGCCTCGGGCGCGATCGGCTCGTTGCCTGCGCTGCTACCGGAAATCtt > 2:166245/1‑136 (MQ=255) tgctaCTCTAAGGTTGGGGGCAGGCCGGCGAACTCCGGCGGCTGGTTTTCGTTGCAGATGGGCATCACCTGGGCGGTACGGGCCTCGGGCGCGATCGGCTCGTTGCCTGCGCTGCTACCGGATATGTTGTATTTGt < 1:35961/132‑1 (MQ=255) gCCGGCGAACACCCGCGGCACGTCTTCGTTGCAGATCGGCATCACCAGCGCGGTACGCGCCTCGGGCGCGATCGGCTCGTTGCCTGCGCTGCTACCGGAAATCTTGTATTTGTCACGCCCGGTGAGCAGCTCGAgg < 2:65397/136‑1 (MQ=255) acCCGCGGCACGTCTTCGTTGCAGATCGGCATCACCAGCGCGGTACGCGCCTCGGGCGCGATCGGCTCGTTGCCTGCGCTGCTACCGGAAATCTTGTATTTGTCACGCCCTGTTATAATCTCGAGGAAGCCCTTCa < 1:113587/136‑1 (MQ=255) acCCGCGGCACGTCTTCGTTGCAGATCGGCATCACCAGCGCGGTACGCGCCTCGGGCGCGATCGGCTCGTTGCCTGCGCTGCTACCGGAAATCTTGTATTTGTCACGCCCGGTGAGCAGCTCGAGGAAGCCCATCa < 2:141519/136‑1 (MQ=255) acCCGCGGCACGTCTTCGTTGCAGATCGGCATCACCAGCGCGGTACGCGCCTCGGGCGCGATCGGCTCGTTGCCTGCGCTGCTACCGGAAATCTTGTATTTGTCACGCCCGGTGAGCAGCTCGAGGAAGCCCATCa < 2:13618/136‑1 (MQ=255) | TGCTCGGCCACGGCAATGTCGGTGTCGTTGGTGTCGCTGAGCACAAAGAAGTCGAACCGGTCGAGGTTGCCGCTGGCGGCCACCGACTCGAACGTTGCGCGCAGGCCGGCGAACACCCGCGGCACGTCTTCGTTGCAGATCGGCATCACCAGCGCGGTACGCGCCTCGGGCGCGATCGGCTCGTTGCCTGCGCTGCTACCGGAAATCTTGTATTTGTCACGCCCGGTGAGCAGCTCGAGGAAGCCCATCA > NC_002947/5728051‑5728300 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |