| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 667,306 | C→T | 26.7% | S1390L (TCG→TTG) | yfhM → | lipoprotein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 667,306 | 0 | C | T | 26.7% | 21.3 / 5.5 | 15 | S1390L (TCG→TTG) | yfhM | lipoprotein |
| Reads supporting (aligned to +/- strand): ref base C (5/6); new base T (2/2); total (7/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01 | |||||||||||
GCAAAGGCTGGCTGGCCGATTATGGCAGCGCCCTGCGTGACCAGGCACTGATCCTGGCGCTGCTGCAGGAAAGCAACCTGGCCAGCAGCCAGGTCGATCAGCGCCTGTTCGCCTTGTCGGATGAACTGGCAGCCAACCGTTGGCTGTCGACCCAAGAGCGCAATGCACTGTTCCTCGCTGGCCGTGGCCTGCTGGGCAAGCCGGAAGGCCAGTGGCAGGCACGCCTGGACAGCGCGGGTGAAGTGCGCGAGTT > NC_002947/667189‑667441 | gCAAAGGCTGGCTGGCCGATTATGGCAGCGCCCTGCGTGACCAGGCACTGATCCTGGCGCTGCTGCAGGAAAGCAACCTGGCCAGCAGCCAGGTCGATCAGCGCCTGTTCGCCTTGTTGGATGAACTGGCAGCCaa > 1:267881/1‑136 (MQ=255)gCAAAGGCTGGCTGGCCGATTATGGCAGCGCCCTGCGTGACCAGGCACTGATCCTGGCGCTGCTGCAGGAAAGCAACCTGGCCAGCAGCCAGGTCGATCAGCGCCTGTTCGCCTTGTCGGATGAACTGGCAGCCaa > 1:58613/1‑136 (MQ=255) cAAAGGCTGGCTGGCCGATTATGGCAGCGCCCTGCGTGACCAGGCACTGATCCTGGCGCTGCTGCAGGAAAGCAACCTGGCCAGCAGCCAGGTCGATCAGCGCCTGTTCGCCTTGTCGGATGAACTGGCAGCCAAc > 1:231080/1‑136 (MQ=255) aaaGGCTGGCTGGCCGATTATGGCAGCGCCCTGCGTGACCAGGCACTGATCCTGGCGCTGCTGCAGGAAAGCAACCTGGCCAGCAGCCAGGTCGATCAGCGCCTGTTCGCCTTGTTGGATGAACTGGCAGCCAAcc > 1:5027/1‑136 (MQ=255) ggCAGCGCCCTGCGTGACCAGGCACTGATCCTGGCGCTGCTGCAGGAAAGCAACCTGGCCAGCAGCCAGGTCGATCAGCGCCTGTTCGCCTTGTTGGATGAACTGGCAGCCAACCGTTGGCTGTCGACCCAAGAgc < 2:5027/136‑1 (MQ=255) ggCAGCGCCCTGCGTGACCAGGCACTGATCCTGGCGCTGCTGCAGGAAAGCAACCTGGCCAGCAGCCAGGTCGATCAGCGCCTGTTCGCCTTGTCGGATGAACTGGCAGCCAACCGTTGGCTGTCGACCCAAGAgc < 1:28521/136‑1 (MQ=255) cgcCCTGCGTGACCAGGCACTGATCCTGGCGCTGCTGCAGGAAAGCAACCTGGCCAGCAGCCAGGTCGATCAGCGCCTGTTCGCCTTGTCGGATGAACTGGCAGCCAACCGTTGGCTGTCGACCCAAGAGCGCAAt < 1:265893/136‑1 (MQ=255) ccTGCGTGACCAGGCACTGATCCTGGCGCTGCTGCAGGAAAGCAACCTGGCCAGCAGCCAGGTCGATCAGCGCCTGTTCGCCTTGTCGGATGAACTGGCAGCCAACCGTTGGCTGTCGACCCAAGAgc < 1:149978/128‑1 (MQ=255) ccTGCGTGACCAGGCACTGATCCTGGCGCTGCTGCAGGAAAGCAACCTGGCCAGCAGCCAGGTCGATCAGCGCCTGTTCGCCTTGTCGGATGAACTGGCAGCCAACCGTTGGCTGTCGACCCAAGAgc > 2:149978/1‑128 (MQ=255) ccTGCGTGACCAGGCACTGATCCTGGCGCTGCTGCAGGAAAGCAACCTGGCCAGCAGCCAGGTCGATCAGCGCCTGTTCGCCTTGTCGGATGAACTGGCAGCCAACCGTTGGCTGTCGACCCAAGAGCGCAATGCa > 2:179560/1‑136 (MQ=255) aCCAGGCACTGATCCTGGCGCTGCTGCAGGAAAGCAACCTGGCCAGCAGCCAGGTCGATCAGCGCCTGTTCGCCTTGTCGGATGAACTGGCAGCCAACCGTTGGCTGTCGACCCAAGAGCGCAATGCACTGTTCCt < 2:231080/136‑1 (MQ=255) gCCAGCAGCCAGGTCGATCAGCGCCTGTTCGCCTTGTTGGATGAACTGGCAGCCAACCGTTGGCTGTCGACCCAAGAGCGCAATGCACTGTTCCTCGCTGGCCGTGGCCTGCTGGGCAAGCCGGAAGGCCAGTggc < 2:267881/136‑1 (MQ=255) gCCAGCAGCCAGGTCGATCAGCGCCTGTTCGCCTTGTCGGATGAACTGGCAGCCAACCGTTGGCTGTCGACCCAAGAGCGCAATGCACTGTTCCTCGCTGGCCGTGGCCTGCTGGGCAAGCCGGAAGGCCAGTggc < 1:179560/136‑1 (MQ=255) cagcCAGGTCGATCAGCGCCTGTTCGCCTTGTCGGATGAACTGGCAGCCAACCGTTGGCTGTCGACCCAAGAGCGCAATGCACTGTTCCTCGCTGGCCGTGGCCTGCTGGGCAAGCCGGAAGGCCAGTggcaggca < 2:58613/136‑1 (MQ=255) cAGCGCCTGTTCGCCTTGTCGGATGAACTGGCAGCCAACCGTTGGCTGTCGACCCAAGAGCGCAATGCACTGTTCCTCGCTGGCCGTGGCCTGCTGGGCAAGCCGGAAGGCCAGTGGCAGGCACGCCTGGACAgcg > 2:115694/1‑136 (MQ=255) cGGATGAACTGGCAGCCAACCGTTGGCTGTCGACCCAAGAGCGCAATGCACTGTTCCTCGCTGGCCGTGGCCTGCTGGGCAAGCCGGAAGGCCAGTGGCAGGCACGCCTGGACAGCGCGGGTGAAGTGCGCGAGtt < 1:30735/136‑1 (MQ=255) | GCAAAGGCTGGCTGGCCGATTATGGCAGCGCCCTGCGTGACCAGGCACTGATCCTGGCGCTGCTGCAGGAAAGCAACCTGGCCAGCAGCCAGGTCGATCAGCGCCTGTTCGCCTTGTCGGATGAACTGGCAGCCAACCGTTGGCTGTCGACCCAAGAGCGCAATGCACTGTTCCTCGCTGGCCGTGGCCTGCTGGGCAAGCCGGAAGGCCAGTGGCAGGCACGCCTGGACAGCGCGGGTGAAGTGCGCGAGTT > NC_002947/667189‑667441 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |