| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 728,963 | C→A | 25.0% | T150N (ACC→AAC) | rluD → | 23S rRNA pseudouridine(1911/1915/1917) synthase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 728,963 | 0 | C | A | 25.0% | 34.1 / 6.5 | 16 | T150N (ACC→AAC) | rluD | 23S rRNA pseudouridine(1911/1915/1917) synthase |
| Reads supporting (aligned to +/- strand): ref base C (7/5); new base A (2/2); total (9/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CAGCGGCACCCTGCTCAACGCGCTGCTGCACCATGTGCCAGACATCGTCAACGTGCCGCGCGCCGGTATCGTGCACCGCCTGGACAAGGACACCACCGGCCTGATGGTGGTGGCCAAGACCCTGCAGGCGCAGACCAAGCTGGTCGAGCAGATGCAGGCCCGCAAGGTCAGCCGCATCTACGAGTGCATCGTGATTGGCGTGGTGACTGCCGGTGGCAAGATCGACGCGCCCATCGGCCGCCACGGCGGCA > NC_002947/728844‑729094 | cAGCGGCACCCTGCTCAACGCGCTGCTGCACCATGTGCCAGACATCGTCAACGGGCCGCGCGCCGGTATCGTGCACCGCCGGGACAAGGACACCACCGGCCTGATGGTGGTGGCCAAGACCCTGCAGGCGCAGAcc < 1:247147/136‑1 (MQ=255) gctgcACCATGTGCCAGACATCGTCAACGTGCCGCGCGCCGGTATCGTGCACCGCCTGGACAAGGACACCACCGGCCTGATGGTGGTGGCCAAGACCCTGCAGGCGCAGACCAAGCTGGTCGAGCAGATGCAGGcc < 2:78418/136‑1 (MQ=255) gcggcACCATGGGCCAGACATCGTCAACGTGCCGCGCGCCGGTATCGTGCACCGCCTGGACAAGGACACCACCGGCCTGATGGTGGTGGCCAAGACCCTGCAGGCGCAGACCAAGCTGGTCGAGCAGATGCAGGcc < 2:285323/133‑1 (MQ=255) cAGACATCGTCAACGTGCCGCGCGCCGGTATCGTGCACCGCCTGGACAAGGACACCACCGGCCTGATGGTGGTGGCCAAGACCCTGCAGGCGCAGACCAAGCTGGTCGAGCAGATGCAGGCCCGCAAGGTCAGCCg > 1:275045/1‑136 (MQ=255) cAACGTGCCGCGCGCCGGTATCGTGCACCGCCTGGACAAGGACACCACCGGCCTGATGGTGGTGGCCAAGACCCTGCAGGCGCAGACCAAGCTGGTCGAGCAGATGCAGGCCCGCAAGGTCAGCCGCATCTACGAg > 2:69161/1‑136 (MQ=255) ccGCGCGCCGGTATCGTGCACCGCCTGGACAAGGACACCACCGGCCTGATGGTGGTGGCCAAGACCCTGCAGGCGCAGACCAAGCTGGTCGAGCAGATGCAGGCCCGCAAGGTCAGCCGCATCTACGAGTGCATCg < 2:234143/136‑1 (MQ=255) gcgcgcCGGTATCGTGCACCGCCTGGACAAGGACACCACCGGCCTGATGGTGGTGGCCAAGACCCTGCAGGCGCAGACCAAGCTGGTCGAGCAGATGCAGGCCCGCAAGGTCAGCCGCATCTACGAGTGCATCGTg < 1:69161/136‑1 (MQ=255) aCCGCCTGGACAAGGACACCACCGGCCTGATGGTGGTGGCCAAGACCCTGCAGGCGCAGACCAAGCTGGTCGAGCAGATGCAGGCCCGCAAGGTCAGCCGCATCTACGAGTGCATCGTGATTGGCGTGGTGACTGc > 1:45220/1‑136 (MQ=255) ggACAAGGACACCACCGGCCTGATGGTGGTGGCCAAGAACCTGCAGGCGCAGACCAAGCt < 1:246135/60‑1 (MQ=255) ggACAAGGACACCACCGGCCTGATGGTGGTGGCCAAGAACCTGCAGGCGCAGACCAAGCt < 1:247586/60‑1 (MQ=255) ggACAAGGACACCACCGGCCTGATGGTGGTGGCCAAGAACCTGCAGGCGCAGACCAAGCt > 2:246135/1‑60 (MQ=255) ggACAAGGACACCACCGGCCTGATGGTGGTGGCCAAGAACCTGCAGGCGCAGACCAAGCt > 2:247586/1‑60 (MQ=255) gCCAAGACCCTGCAGGCGCAGACCAAGCTGGTCGAGCAGATGCAGGCCCGCAAGGTCAGCCGCATCTACGAGTGCATCGTGATTGGCGTGGTGACTGCCGGTGGCAAGATCGACGCGCCCATCGGCCGCCAcggcg > 1:125894/1‑136 (MQ=255) gCCAAGACCCTGCAGGCGCAGACCAAGCTGGTCGAGCAGATGCAGGCCCGCAAGGTCAGCCGCATCTACGAGTGCATCGTGATTGGCGTGGTGACTGCCGGTGGCAAGATCGACGCGCCCATCGGCCGCCAcggcg > 2:29147/1‑136 (MQ=255) gCCAAGACCCTGCAGGCGCAGACCAAGCTGGTCGAGCAGATGCAGGCCCGCAAGGTCAGCCGCATCTACGAGTGCATCGTGATTGGCGTGGTGACTGCCGGTGGCAAGATCGACGCGCCCATCGGCCGCCAcggcg > 2:8904/1‑136 (MQ=255) aaGACCCTGCAGGCGCAGACCAAGCTGGTCGAGCAGATGCAGGCCCGCAAGGTCAGCCGCATCTACGAGTGCATCGTGATTGGCGTGGTGACTGCCGGTGGCAAGATCGACGCGCCCATCGGCCGCCACGGCGGCa > 1:275343/1‑136 (MQ=255) | CAGCGGCACCCTGCTCAACGCGCTGCTGCACCATGTGCCAGACATCGTCAACGTGCCGCGCGCCGGTATCGTGCACCGCCTGGACAAGGACACCACCGGCCTGATGGTGGTGGCCAAGACCCTGCAGGCGCAGACCAAGCTGGTCGAGCAGATGCAGGCCCGCAAGGTCAGCCGCATCTACGAGTGCATCGTGATTGGCGTGGTGACTGCCGGTGGCAAGATCGACGCGCCCATCGGCCGCCACGGCGGCA > NC_002947/728844‑729094 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |