| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 739,872 | C→T | 15.4% | R253Q (CGG→CAG) | pilC ← | type IV fimbrial assembly protein PilC |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 739,872 | 0 | C | T | 15.4% | 61.2 / 4.5 | 26 | R253Q (CGG→CAG) | pilC | type IV fimbrial assembly protein PilC |
| Reads supporting (aligned to +/- strand): ref base C (13/9); new base T (2/2); total (15/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.43e-01 | |||||||||||
ACCCGCGCCACCGTCCCCAGCGCATCCAGCAACGGCACCCCGGCGCCGTAGGACGTTGCCAGGCTGCGCGCAAACCGCGCCAGCGCGGCCTGCCCCAGCAACTTGCCAAACACGGGCAAGCCCAGCACCCGTCGAGATATCCACAGGCGCGCTGGCGCGTGCTGCCGGTAAAGCTGGCGCACGGCAACACCCATCACCACGCCCATCACCAGCAGCAGCGGCGCAAACCGGCTCAGCCCTGTGGACAAGTTGATCAC > NC_002947/739743‑739999 | aCCCGCGCCACCGTCCCCAGCGCATCCAGCAACGGCACCCCGGCGCCGTAGGACGTTGACAGGCTGCGCGCAAACCGCGCCAGCGCGCCCTGCCCCAGCAACTTGCCAAACACGGGCAAGCCCAGCACCCGTCgag > 1:283393/1‑136 (MQ=255) ccGCGCCACCGTCCCCAGCGCATCCAGCAACGGCACCCCGGCGCCGTAGGACGTTGCCAGGCTGCGCGCAAACCGCGCCAGCGCGGCCTGCCCCAGCAACTTGCCAAACACGGGCAAGCCCAGCACCCGTCGAGat > 2:228256/1‑136 (MQ=255) cACCGTCCCCAGCGCATCCAGCAACGGCACCCCGGCGCCGTAGGACGTTGCCAGGCTGCGCGCAAACCGCGCCAGCGCGGCCTGCCCCAGCAACTTGCCAAACACGGGCAAGCCCAGCACCCGTCGAGATATCcac > 2:257238/1‑136 (MQ=255) aGCGCATCCAGCAACGGCACCCCGGCGCCGTAGGACGTTGCCAGGCTGCGCGCAAACCGCGCCAGCGCGGCCTGCCCCAGCAACTTGCCAAACACGGGCAAGCCCAGCACCCGTCGAGATATCCACAGGCGCGCTg > 1:175763/1‑136 (MQ=255) tCCAGCAACGGCACCCCGGCGCCGTAGGACGTTGCCAGGCTGCGCGCAAACCGCGCCAGCGCGGCCTGCCCCAGCAACTTGCCAAACACGGGCAAGCCCAGCACCCGTCGAGATATCCACAGGCGCGCTGGCGCGt > 1:90061/1‑136 (MQ=255) cccGGCGCCGTAGGACGTTGCCAGGCTGCGCGCAAACCGCGCCAGCGCGGCCTGCCCCAGCAACTTGCCAAACACGGGCAAGCCCAGCACCCGTCGAGATATCCACAGGCGCGCTGGCGCGTGCTGCCGGTAAAGc < 1:204282/136‑1 (MQ=255) cccGGCGCCGTAGGACGTTGCCAGGCTGCGCGCAAACCGCGCCAGCGCGGCCTGCCCCAGCAACTTGCCAAACACGGGCAAGCCCAGCACCCGTCGAGATATCCACAGGCGCGCTGGCGCGTGCTGCCGGTAAAGc < 1:257204/136‑1 (MQ=255) gacagcCGTAGGACGTTGCCAGGCTGCGCGCAAACCGCGCCAGCGCGGCCTGCCCCAGCAACTTGCCAAACACGGGCAAGCCCAGCACCCGTCGAGATATCCACAGGCGCGCTGGCGCGTGCTGCCGGTAAAGCTg < 1:95391/132‑1 (MQ=255) cGGCGCCGTAGGACGTTGCCAGGCTGCGCGCAATCCGCGCCAGCGCGGCCTGCCCCAGCTACTTGCCAATCACGGGCAAGCCCAGCACGCGTCGAGATTTCCACAGGCGCGCTGGCGCGTGCTGCCGGTAAAGCTg < 2:182725/136‑1 (MQ=255) tGCCAGGCTGCGCGCAAACCGCGCCAGCGCGGCCTGCCCCAGCAACTTGCCAAACACGGGCAAGCCCAGCACCCGTCGAGATATCCACAGGCGCGCTGGCGCGTGCTGCCGGTAAAGCTGGCGCACGGCAACAccc > 2:52175/1‑136 (MQ=255) ccAGGCTGCGCGCAAACCGCGCCAGCGCGGCCTGCCCCAGCAACTTGCCAAACACGGGCAAGCCCAGCACCCGACGAGATATCCACAGGCGCGCTGGCGCGTGCTGCCGGTAAAGCTGGCGCACGGCAACACCCAt > 2:77806/1‑136 (MQ=255) cAGGCTGCGCGCAAACCGCGCCAGCGCGGCCTGCCCCAGCAACTTGCCAAACACGGGCAAGCCCAGCGCCCGTCGAGATATCCACAGGCGCGCTGGCGCGTGCTGCCGGTAAAGCTGGCGCACGGCAACACCCATc > 1:59123/1‑136 (MQ=255) cAGGCTGCGCGCAAACCGCGCCAGCGCGGCCTGCCCCAGCAACTTGCCAAACACGGGCAAGCCCAGCACCTGTCGAGATATCCACAGGCGCGCTGGCGCGTGCTGCCGGTAAAGCTGGCGCACGGCAACACCCATc > 1:67700/1‑136 (MQ=255) cAGGCTGCGCGCAAACCGCGCCAGCGCGGCCTGCCCCAGCAACTTGCCAAACACGGGCAAGCCCAGCACCTGTCGAGATATCCACAGGCGCGCTGGCGCGTGCTGCCGGTAAAGCTGGCGCACGGCAACACCCATc > 1:66315/1‑136 (MQ=255) cAGGCTGCGCGCAAACCGCGCCAGCGCGGCCTGCCCCAGCAACTTGCCAAACACGGGCAAGCCCAGCACCCGTCGAGATATCCACAGGCGCGCTGGCGCGTGCTGCCGGTAAAGCTGGCGCACGGCACCACCCATc > 2:22315/1‑136 (MQ=255) ccAGCAACTTGCCAAACACGGGCAAGCCCAGCGCCCGTCGAGATATCCACAGGCGCGCTGGCGCGTGCTGCCGGTAAAGCTGGCGCACGGCAACACCCATCACCACGCCCATCACCAGCAGCAGCGGCGCAAACCg < 2:59123/136‑1 (MQ=255) ccAGCAACTTGCCAAACACGGGCAAGCCCAGCACCTGTCGAGATATCCACAGGCGCGCTGGCGCGTGCTGCCGGTAAAGCTGGCGCACGGCAACACCCATCACCACGCCCATCACCAGCAGCAGCGGCGCAAACCg < 2:66315/136‑1 (MQ=255) ccAGCAACTTGCCAAACACGGGCAAGCCCAGCACCTGTCGAGATATCCACAGGCGCGCTGGCGCGTGCTGCCGGTAAAGCTGGCGCACGGCAACACCCATCACCACGCCCATCACCAGCAGCAGCGGCGCAAACCg < 2:67700/136‑1 (MQ=255) aGCAACTTGCCAAACACGGGCAAGCCCAGCACCCGTCGAGATATCCACAGGCGCGCTGGCGCGTGCTGCCGGTAAAGCTGGCGCACGGCAACACCCATCACCACGCCCATCACCAGCAGCAGCGGCGCAAACCGGc < 1:16766/136‑1 (MQ=255) aaCTTGCCAAACACGGGCAAGCCCAGCACCCGTCGAGATATCCACAGGCGCGCTGGCGCGTGCTGCCGGTAAAGCTGGCGCACGGCAACACCCATCACCACGCCCATCACCAGCAGCAGCGGCGCAAACCGGCTCa < 1:257238/136‑1 (MQ=255) aaaCACGGGCAAGCCCAGCACCCGTCGAGATATCCACAGGCGCGCTGGCGCGTGCTGCCGGTAAAGCTGGCGCACGGCAACACCCATCACCACGCCCATCACCAGCAGCAGCGGCGCAAACCGGCTCAGCCCtgtg > 1:170578/1‑136 (MQ=255) gCAAGCCCAGCACCCGTCGAGATATCCACAGGCGCGCTGGCGCGTGCTGCCGGTAAAGCTGGCGCACGGCAACACCCATCACCACGCCCATCACCAGCAGCAGCGGCGCAAACCGGCTCAGCCCTGTGGACAAGtt < 1:52175/136‑1 (MQ=255) aGCCCAGCACCCGTCGAGATATCCACAGGCGCGCTGGCGCGTGCTGCCGGTAAAGCTGGCGCACGGCAACACCCATCACCACGCCCATCACCAGCAGCAGCGGCGCAAACCGGCTCAGCCCTGTGGACAAGTTGAt > 2:15277/1‑136 (MQ=255) aGCCCAGCACCCGTCGAGATATCCACAGGCGCGCTGGCGCGTGCTGCCGGTAAAGCTGGCGCACGGCAACACCCATCACCACGCCCATCACCAGCAGCAGCGGCGCAAACCGGCTCAGCCCTGTGGACAAGTTGAt > 2:245592/1‑136 (MQ=255) aGCCCAGCACCCGTCGAGATATCCACAGGCGCGCTGGCGCGTGCTGCCGGTAAAGCTGGCGCACGGCAACACCCATCACCACGCCCATCACCAGCAGCAGCGGCGCAAACCGGCTCAGCCCTGTGGACAAGTTGAt > 2:257784/1‑136 (MQ=255) ccAGCACCCGTCGAGATATCCACAGGCGCGCTGGCGCGTGCTGCCGGTAAAGCTGGCGCACGGCAACACCCATCACCACGCCCATCACCAGCAGCAGCGGCGCAAACCGGCTCAGCCCTGTGGACAAGTTGATCAc < 1:228256/136‑1 (MQ=255) | ACCCGCGCCACCGTCCCCAGCGCATCCAGCAACGGCACCCCGGCGCCGTAGGACGTTGCCAGGCTGCGCGCAAACCGCGCCAGCGCGGCCTGCCCCAGCAACTTGCCAAACACGGGCAAGCCCAGCACCCGTCGAGATATCCACAGGCGCGCTGGCGCGTGCTGCCGGTAAAGCTGGCGCACGGCAACACCCATCACCACGCCCATCACCAGCAGCAGCGGCGCAAACCGGCTCAGCCCTGTGGACAAGTTGATCAC > NC_002947/739743‑739999 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |