| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,681,655 | G→A | 21.0% | L44L (CTG→CTA) | recJ → | ssDNA‑specific exonuclease RecJ |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,681,655 | 0 | G | A | 21.0% | 35.3 / 4.0 | 19 | L44L (CTG→CTA) | recJ | ssDNA‑specific exonuclease RecJ |
| Reads supporting (aligned to +/- strand): ref base G (7/8); new base A (2/2); total (9/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ACCTCGCCCGCTGCCGCCGACCCTGCCATTTCTGGGTAACCTGCCACCCTTGCTGACCCGCCTTTACGCCGCGCGCGGCGTGCAGAGCGAGGCCGAGCTGGACAAGAGCCTGGCACGCCTGCTGCCCTACCAGCAGCTCAAGGGCATCGAGGCCGGTGTGGACCTGCTGGTCGAAGCCATCGACCAGCGCCAGCGCATCCTCATCGTCGGCGACTTCGATGCCGACGGTGCCACCGCCAGCACCGT > NC_002947/1681535‑1681780 | agcTCGCCCGCTGCCGCAGACCCTGCCATTTCTGGTTAACCTGACACCCTTGCTGACCCGCCTTTACGCCGGGCGCGGCGTGCAGAGCGAGGCCGAGCTGGACAAGAGCCTGGCACGCCTGCTGCCCTACcagcag < 2:33982/134‑1 (MQ=255) cGCTGCCGCCGACCCTGCCATTTCTGGGTAACCTGCCACCCTTGCTGACCCGCCTTTACGCCGCGCGCGGCGTGCAGAGCGAGGCCGCGCTGGACAAGAGCCTGGCCCGCCTACTGCCCTACCAGCAGCTCAAggg > 1:214140/1‑136 (MQ=255) cGCTGCCGCCGACCCTGCCATTTCTGGGTAACCTGCCACCCTTGCTGACCCGCCTTTACGCCGCGCGCGGCGTGCAGAGCGAGGCCGAGCTGGACAAGAGCCTGGCACGCCTACTGCCCTACCAGCAGCTCAAggg > 1:212641/1‑136 (MQ=255) cgccgACCCTGCCATTTCTGGGTAACCTGCCACCCTTGCTGACCCGCCTTTACGCCGCGCCCGGCGTGCAGAGCGAGGCCGAGCTGGACAAGAGCCTGGCACGCCTGCTGCCCTACCAGCAGCTCAAGGGCATCGa > 1:286569/1‑136 (MQ=255) ccgACCCTGCCATTTCTGGGTAACCTGCCCCCCTTGCTGACCCGCCTTTACGCCGCGCGCGGCGTGCAGAGCGAGGCCGAGCTGGACAAGAGCCTGGCACGCCTGCTGCCCTACCAGCAGCTCAAGGGCATCGAgg < 2:209648/136‑1 (MQ=255) gCCTTTACGCCGCGCGCGGCGTGCAGAGCGAGGCCGAGCTGGACAAGAGCCTGGCACGCCTGCTGCCCTACCAGCAGCTCAAGGGCATCGAGGCCGGTGTGGACCTGCTGGTCGAAGCCATCGAccagcgccagcg > 2:150740/1‑136 (MQ=255) cgccgcGCGCGGCGTGCAGAGCGAGGCCGAGCTGGACAAGAGCCTGGCACGCCTGCTGCCCTACCAGCAGCTCAAGGGCATCGAGGCCGGTGTGGACCTGCTGGTCGAAGCCATCGACCAGCGCCAGCGCATCCTc < 2:186595/136‑1 (MQ=255) gcggcgTGCAGAGCGAGGCCGAGCTGGACAAGAGCCTGGCACGCCTGCTGCCCTACCAGCAGCTCAAGGGCATCGAGGCCGGTGTGGACCTGCTGGTCGAAGCCATCGACCAGCGCCAGCGCATCCTCATCGTCgg > 1:104628/1‑136 (MQ=255) gCCGAGCTGGACAAGAGCCTGGCACGCCTGCTGCCCTACCAGCAGCTCAAGGGCATCGAGGCCGGTGTGGACCTGCTGGGCGAAGCCATCGCCCCGCGCCAGCGGATCCTCATCGTCGGCGACGTCGCTGCCgccc > 1:39654/1‑133 (MQ=255) cGAGCTGGACAAGAGCCTGGCACGCCTGCTGCCCTACCAGCAGCTCAAGGGCATCGAGGCCGGTGTGGACCTGCTGGTCGAAGCCATCGACCAGCGCCAGCGCATcc > 1:201446/1‑107 (MQ=255) cGAGCTGGACAAGAGCCTGGCACGCCTGCTGCCCTACCAGCAGCTCAAGGGCATCGAGGCCGGTGTGGACCTGCTGGTCGAAGCCATCGACCAGCGCCAGCGCATcc < 2:201446/107‑1 (MQ=255) cGAGCTGGACAAGAGCCTGGCACGCCTGCTGCCCTACCAGCAGCTCAAGGGCATCGAGGCCGGTGTGGACCTGCTGGTCGAAGCCATCGACCAGCGCCAGCGCATCCTCATCGTCGGCGACTTCGATGCCGACGGt > 1:172812/1‑136 (MQ=255) cGAGCTGGACAAGAGCCTGGCACGCCTGCTGCCCTACCAGCAGCTCAAGGGCATCGAGGCCGGTGTGGACCTGCTGGTCGAAGCCATCGACCAGCGCCAGCGCATCCTCATCGTCGGCGACTTCGATGCCGACGGt > 1:240737/1‑136 (MQ=255) cGAGCTGGACAAGAGCCTGGCACGCCTGCTGCCCTACCAGCAGCTCAAGGGCATCGAGGCCGGTGTGGACCTGCTGGTCGAAGCCATCGACCAGCGCCAGCGCATCCTCATCGTCGGCGACTTCGATGCCGACGGt < 2:250888/136‑1 (MQ=255) aCAAGAGCCTGGCACGCCTGCTGCCCTACCAGCAGCTCAAGGGAATCGAGGCCGGTGTGGACCTGCTGGTCGAAGCCATCGACCAGCGCCAGCGCATCCTCATCGTCGGCGACTTCGATGCCGACGGTGCCACCGc < 1:109323/136‑1 (MQ=255) agagCCTGGCACGCCTGCGGCCCTACCAGCAGCTCAAGGGCATCGAGGCCGGTGTGGACCTGCTGGTCTAAGCCATCCACCAGCGCCAGCGCATCCTCATCGTCGGCGACTTCGATGCCGACGGTGCCACCGCCAg < 1:123986/136‑1 (MQ=255) cTGGCACGCCTACTGCCCTCCCAGCAGCTCACGGGCATCGCGGCCGGTGTGGACCTGCTGGTCGAAGCCATCGCCCAGCGCCAGCGCATCCTCATCGTCGGCGACTTCGATGCCGACGGTGCCACCGCCAGCACCg < 2:214140/136‑1 (MQ=255) cTGGCACGCCTACTGCCCTACCAGCAGCTCAAGGGCATCGAGGCCGGTGTGGACCTGCTGGTCGAAGCCATCGACCAGCGCCAGCGCATCCTCATCGTCGGCGACTTCGATGCCGACGGTGCCACCGCCAGCACCg < 2:212641/136‑1 (MQ=255) gggCACGCCTGCTGCCCTACCAGCAGCTCAAGGGCATCGAGGCCGGTGTGGACCTGCTGGTCGAAGCCATCGACCAGCGCCAGCGCATCCTCATCGTCGGCGACTTCGATGCCGACGGTGCCACCGCCAGCACCGt < 1:150740/135‑1 (MQ=255) | ACCTCGCCCGCTGCCGCCGACCCTGCCATTTCTGGGTAACCTGCCACCCTTGCTGACCCGCCTTTACGCCGCGCGCGGCGTGCAGAGCGAGGCCGAGCTGGACAAGAGCCTGGCACGCCTGCTGCCCTACCAGCAGCTCAAGGGCATCGAGGCCGGTGTGGACCTGCTGGTCGAAGCCATCGACCAGCGCCAGCGCATCCTCATCGTCGGCGACTTCGATGCCGACGGTGCCACCGCCAGCACCGT > NC_002947/1681535‑1681780 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |