| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,154,248 | 0 | G | C | 52.6% | ‑3.4 / 10.2 | 19 | T146T (ACG→ACC) | PP_2768 | branched‑chain amino acid ABC transporter permease |
| Reads supporting (aligned to +/- strand): ref base G (7/2); new base C (10/0); total (17/2) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.11e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.26e-01 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TACATCATCGAGGGCGCCGCCCAGGCCTTATGGGGCGCCCAGGTTCACGGCCTGGAGCTGGCCATCAGCGACGCCCCGCTGGAGCTGGGCGGCCTGCTGCTGTCGCAGTTCGACCTGTT‑CGCCGCCGCAACGGCGGC‑GGCCCTGGTGGTGCTTCTGTCCTTGCTGTTCAACCGCACGCGCATCGGCCTGGCGCTGCGTGCGGTGGCCGACGATCCCCGCGCGGCGATGAGCGTCGGTATCCGCCTGCCGAGGGTGTGGGC > NC_002947/3154117‑3154376 | tACATCATCGAGGGCGCCGCCCAGGCCTTATGGGGCGCCCAGGTTCACGGCCTGGAGCTGGCCATCAGCGACGCCCCGCTGGAGCTGGGCGGCCTGCTGCTGTCGCAGTTCGACCTGTT‑CGGCGCCCCCAccgcgg > 2:4185130/1‑136 (MQ=255)tACATCATCGAGGGCGCCGCCCAGGCCTTATGGGGCGCCCAGGTTCACGGCCTGGAGCTGGCCATCAGCGACGCCCCGCTGGAGCTGGGCGGCCTGCTGCTGTCGCAGTTCGACCTGTT‑CGGCCCCCCCAccgcgg > 1:3559594/1‑136 (MQ=255) tcatcGAGGGCGCCGCCCAGGCCTTATGGGGCGCCCAGGTTCACGGCCTGGAGCTGGCCATCAGCGACGCCCCGCTGGAGCTGGGCGGCCTGCTGCTGTCGCAGTTCGCCCTGTT‑CGCCGCCGCCAccgcggc‑ggg > 2:1121506/1‑135 (MQ=255) catcGAGGGCGCCGCCCAGGCCTTATGGGGCGCCCAGGTTCACGGCCTGGAGCTGGCCATCAGCGACGCCCCGCTGGAGCTGGGCGGCCTGCTGCTGTCGCAGTTCGACCTGTT‑CGCCCCCCCCAccgcggg‑gggc > 2:4321232/1‑134 (MQ=255) aGGGCGCCGCCCAGGCCTTATGGGGCGCCCAGGTTCACGGCCTGGAGCTGGCCATCAGCGACGCCCCGCTGGAGCTGGGCGGCCTGCTGCTGTCGCAGTTCGACCTGTT‑CGGCGCCCCCACCGCGGC‑GGCCCcggg > 2:2985762/1‑135 (MQ=255) aGGGCGCCGCCCAGGCCTTATGGGGCGCCCAGGTTCACGGCCTGGAGCTGGCCATCAGCGACGCCCCGCTGGAGCTGGGCGGCCTGCTGCTGTCGCAGTTCGACCTGTT‑CGGCCCCCCCACCGCGGG‑GGGCCcggg > 1:2379775/1‑135 (MQ=255) aGGGCGCCGCCCAGGCCTTATGGGGCGCCCAGGTTCACGGCCTGGAGCTGGCCATCAGCGACGCCCCGCTGGAGCTGGGCGGCCTGCTGCTGTCGCAGTTCGACCTGTT‑CGCCCCCCCCACCGCGGC‑GGGCCcggg > 2:3555754/1‑135 (MQ=255) aGGGCGCCGCCCAGGCCTTATGGGGCGCCCAGGTTCACGGCCTGGAGCTGGCCATCAGCGACGCCCCGCTGGAGCTGGGCGGCCTGCTGCTGTCGCAGTTCGACCTGTT‑CGCCCCCCCCACCGCGGC‑GGCCCgggg > 1:3019191/1‑135 (MQ=255) aGGGCGCCGCCCAGGCCTTATGGGGCGCCCAGGTTCACGGCCTGGAGCTGGCCATCAGCGACGCCCCGCTGGAGCTGGGCGGCCTGCTGCTGTCGCAGTTCGACCTGTT‑CGCCCCCCCCACCGCGGC‑GGCCCcggg > 1:1929541/1‑135 (MQ=255) gggCGCCGCCCAGGCCTTATGGGGCGCCCAGGTTCACGGCCTGGAGCTGGCCATCAGCGACGCCCCGCTGGAGCTGGGCGGCCTGCTGCTGTCGCAGTTCGACCTGTT‑CGCCCCCGCACCGGCGGC‑GGGCCggggg > 2:1161629/1‑134 (MQ=255) gggCGCCGCCCAGGCCTTATGGGGCGCCCAGGTTCACGGCCTGGAGCTGGCCATCAGCGACGCCCCGCTGGAGCTGGGCGGCCTGCTGCTGTCGCAGTTCGACCTGTT‑CGCCCCCCCCACCGCGGG‑GGgcccgctg > 2:3059341/1‑131 (MQ=255) ccAGGCCTTATGGGGCGCCCAGGTTCACGGCCTGGAGCTGGCCATCAGCGACGCCCCGCTGGAGCTGGGCGGCCTGCTGCTGTCGCAGTTCGACCTGTT‑CGCCGCCGCACCCGCGGCGGGCCCGGggggtggtggg > 2:3074051/1‑131 (MQ=255) gggCGCCCAGGTTCACGGCCTGGAGCTGGCCATCAGCGACGCCCCGCTGGAGCTGGGCGGCCTGCTGCTGTCGCAGTTCGACCTGTT‑CGCCCCCCCAACCGCGGC‑GGCCCGGGTGGTGCGTCTGGCCGTGCTGtta > 2:2675106/1‑135 (MQ=255) cccAGGTTCACGGCCTGGAGCTGGCCATCAGCGACGCCCCGCTGGAGCTGGGCGGCCTGCTGCTGTCGCAGTTAGACCTGTT‑CGCCGCCGCAACGGCGGC‑GGGCCCGGGGGGGGGTGGGTGCGTGGTGTTCAAccc > 2:1183619/1‑135 (MQ=255) ccAGGTTCACGGCCTGGAGCTGGCCATCAGCGACGCCCCGCTGGAGCTGGGCGGCCTGCTGCTGTCGCAGTTCGACCTGTT‑CGCCGCCGCCACGGCGGC‑GGCCCGGGGGGGGGGTGTGTGCGTGGTGTTGAAcccc > 1:1086590/1‑134 (MQ=255) cAGGTTCACGGCCTGGAGCTGGCCATCAGCGACGCCCCGCTGGAGCTGGGTGGCCTGCTGCTGTCGCAGTTCGACCTGTT‑CGCCCCCGCCACCGCCGC‑GGCCCGGGTGGTGGGTATGTGCTTGCTGTTGAAccccc > 2:4562703/1‑133 (MQ=255) ccTGGAGCTGGCCATCAGCGACGCCCCGCTGGAGCTGGGCGGCCTGCTGCTGTCGCAGGTGGACCTGTTCCGCCCCCCCAACGG‑GGC‑GGGGCGGGGGGTGGTTAGGTCGCTGCGGTTAAACCCCCCgcgccagggc > 2:1055827/1‑130 (MQ=255) aTCAGCGACGCCCCGCTGGAGCTGGGCGGCCTGCTGCTGTCGCAGTTCGACCTGTT‑CGCCCCCCCCACCGCGGC‑GGCCCGGGTGGGGCGTGTGTGCGTGGGGTTGAACCCCCCCCCCAGCGCCCcggcgcggcggg > 1:1821620/1‑134 (MQ=255) ccccGCTGGAGCTGGGCGGCCTGCTGCTGTCGCAGTTCGACCTGTT‑CGCCGCCGCAACGGCGGC‑GGCCCGGGTGGGGGTTGTGTGCTTGCTGTTCAACCCCCCGCCCCTCGGCCTGGGGCTGCGTGGGGTGGCcgc > 2:1158876/1‑135 (MQ=255) ccccGCTGGAGCTGGGCGGCCTGCTGCTGTCGCAGTTCGACCTGTT‑CGCCGCCCCCACGGGGGC‑GGCCCGGGGGGGGCGTCGGTGCGTGCGGTTGAACCGCACGCGCATCGGCCTGGCGCAGCGTGCGGGGGCcgc > 2:3144604/1‑135 (MQ=255) gctgctgTCGCAGTTCGACCTGTT‑CGCCGCCGCAACGGCAGC‑GGCCCTGGTGGTGCTTATGTCCTTGCTGTTCAACCGCACCCGCAACGGCCTGGCGCTGCGTGCGGTGGCCGCCGAACCCCGCGCGGCGATGAGc > 2:1005228/1‑136 (MQ=255) tt‑CGCCGCCGCAACGGCGGC‑GGCCCTGGTGGTGCTTCTGTCCTTGCTGTTCAACCGCACGCGCATCGGCCTGGCGCTGCGTGCGGTGGCCGACGATCCCCGCGCGGCGATGAGCGTCGGTATCCGCCTGCCGAggg < 2:3148858/136‑1 (MQ=255) ccgcAACGGCGGT‑GGCCCTGGTGGTGCTTCTGTCCTTGCTGTTCAACCGCACGCGCATCGGCCTGGCGCTGCGTGCGGTGGCCGACGATCCCCGCGCGGCGATGAGCGTCGGTATCCGCCTGCCGAGGGTGTGGGc < 1:1518130/136‑1 (MQ=255) | TACATCATCGAGGGCGCCGCCCAGGCCTTATGGGGCGCCCAGGTTCACGGCCTGGAGCTGGCCATCAGCGACGCCCCGCTGGAGCTGGGCGGCCTGCTGCTGTCGCAGTTCGACCTGTT‑CGCCGCCGCAACGGCGGC‑GGCCCTGGTGGTGCTTCTGTCCTTGCTGTTCAACCGCACGCGCATCGGCCTGGCGCTGCGTGCGGTGGCCGACGATCCCCGCGCGGCGATGAGCGTCGGTATCCGCCTGCCGAGGGTGTGGGC > NC_002947/3154117‑3154376 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |