Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,160,4320TG59.6% 3.5 / 51.3 104L159R (CTG→CGG) PP_3661membrane protein
Reads supporting (aligned to +/- strand):  ref base T (10/28);  new base G (38/18);  total (58/46)
Fisher's exact test for biased strand distribution p-value = 1.25e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.15e-01
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 110 total aligned reads displayed.

ACCAGTACCCTGCTGATCGGCCTGTGGTGTGGCATGAAAGTGTTCAAGCTCGACCGCGATACCGCCTTGCTGACCGCCGCCGGCAGCGCCATCTGCGGCGCCGCCGCCGTGCTGGCATTCGAATCGGCGCTGCGCAG‑CGCCCCCCATAAAAGCGCCATGGCAGTCGGAAGCGTAGTGCTGTTCGGTACCTTGTCGATGTTCCTTTACCCACTGGCGATCAATGCCGGCTGGCTGCATCTGGATACCATGGGCGCCGGGCTG  >  NC_002947/4160302‑4160562
                                                                                                                                  |                                                                                                                                   
ccctgTACCGTGCTGATGGGCGTGTGGGGGGGAATGAACGGGTGCATGCGCGACCGCGATACCGCCTTGCTGACGGCCGCGGGCAGCGCTAGCTGCGGCGCCGCCGGGGTGCTGGCATTCGAATGGGCGCTgcgct                                                                                                                                <  2:1804154/132‑2 (MQ=255)
aCCTGTACCCTGCTGAGCGGCCTGTGGTGTGTCATTAATGTTTTAAAGCTCGACCGCTATACCGCCTTGCTGCCCGCCGCCGGCAGCGCCTTGGGGGGCGCCGCCGCGGGGGTGGCATTCGAATCggcgcggcgcc                                                                                                                                <  2:2765969/136‑2 (MQ=255)
aCCAGTACCCTGCTGATCGGCCTGTGGTGTGGCATGAAAGTGTTCAAGCTCGACCGCGATACCGCCTTGCTGACCGCCGCCGGCAGCGCCATCTGCGGCGCCGCCGCCGTGCTGGCATTCGAATCGGGGCTgcgcc                                                                                                                                >  2:2428767/1‑135 (MQ=255)
aCCAGTACCCTGCTGATCGGCCTGTGGTGTGGCATGAAAGTGTTCAAGCTCGACCGCGATACCGCCTTGCTGACCGCCGCCGGCAGCGCCATCTGCGGCGCCGCCGCCGTGCTGGCATTCGAATCGGCGCTGCGCa                                                                                                                                <  1:1965366/136‑1 (MQ=255)
aCCAGTACCCTGCTGATCGGCCTGGGGGGTGGCATGAAAGTTTTCAAGCTCGACCGCTATACCGCCTTGCTGACCGCCGCCGGCAGCTCCATCTGCGGCGCCGCCGCCGTGCTGGCATTCGAATCGGCACTGCGCa                                                                                                                                <  1:3846376/136‑1 (MQ=255)
  cAGTACCCTGCTGATCGGCCTGTGGTGTGGCATGAAAGTGTTCAAGCTCGACCGCGATACCGCCTTGCTGACCGCCGCCGGCAGCGCCATCTGCGGCGCCGCCGCCGTGCTGGCATTCGAATCGGTGCTGCGCAg‑c                                                                                                                             >  2:3943871/1‑136 (MQ=255)
  cAGTACCCTGCTGATCGGCCTGTGGTGTGGCATGAAAGTGTTCAAGCTCGACCGCGATACCGCCTTGCTGACCGCCGCCGGCAGCGCCATCTGCGGCGCCGCCGCCGTGCTGGCATTCGAATCGGCGCGGcgccg‑c                                                                                                                             >  2:1020361/1‑136 (MQ=255)
  cAGTACCCTGCTGATCGGCCTGTGGTGTGGCATGAAAGTGTTCAAGCTCGACCGCGATACCGCCTTGCTGACCGCCGCCGGCAGCGCCATCTGCGGCGCCGCCGCCGTGCTGGCATTCGAATCGGCGCGGCGCAg‑c                                                                                                                             >  1:954411/1‑136 (MQ=255)
  aaGTACCCGGCTGAGCGGCGTGGGGGGTGGAATCAATGTGTTCAAGCGCGACCGCGAAACCGCCTGGCTGCCCGCCGCCGGCAGCGCCATCTGCGGCGCCGCCGCCGTGCTGGCATTCTAATCGGCGCTGCGCAg‑c                                                                                                                             <  2:2351381/135‑1 (MQ=255)
     tACCCTGCTGATCGGCCTGTGGTGTGGCATGAAAGTGTTCAAGCTCGACCGCGATACCGCCTTGCTGACCGCCGCCGGCAGCGCCATCTGCGGCGCCGCCGCCGTGCTGGCATTCGAATCGGGGCGGCGGCG‑CGcc                                                                                                                          >  1:2670087/1‑136 (MQ=255)
       cccTGCTGATCGGCCTGTGGTGTGGCATGAAAGTGTTCAAGCTCGACCGCGATACCGCCTTGCTGACCGCCGCCGGCAGCGCCATCTGCGGCGCCGCCGCCGTGCTGGCATTCGAATCGGCGGGGCGCCG‑cccccc                                                                                                                        >  2:1587071/1‑136 (MQ=255)
          tgctgATCGGCCTGTGGTGTGGCATGAAAGTGTTCAAGCTCGACCGCGATACCGCCTTGCTGACCGCCGCCGGCAGCGCCATCTGCGGCGCCGCCGCCCTGCTGGCATTCGAATCGGGGCGGCGGAC‑ccccccccc                                                                                                                     >  1:23450/1‑135 (MQ=255)
             tgATCGGCCTGTGGTGTGGCATGAAAGTGTTCAAGCTCGACCGCGATACCGCCTTGCTGACCGCCGCCGGCAGCGCCATCTGCGGCGCCGCCGCCGTGCTGGCATTCGAATCGGGGGGGGGGGG‑CGccccccccaa                                                                                                                  >  1:2876918/1‑132 (MQ=255)
             tgATCGGCCTGTGGTGTGGCATGAAAGTGTTCAAGCTCGACCGCGATACCGCCTTGCTGACCGCCGCCGGCAGCGCCATCTGCGGCGCCGCCGCCGTGCTGGCATTCGAATCGGGGGGGGGCCG‑ccccccccccca                                                                                                                  >  1:1361701/1‑132 (MQ=255)
             tgATCGGCCTGTGGTGTGGCATGAAAGTGTTCAAGCTCGACCGCGATACCGCCTTGCTGACCGCCGCCGGCAGCGCCATCTGCGGCGCCGCCGCCGTGCTGGCATTCGAATCGGGGGGGGGCCG‑ccccccccccaa                                                                                                                  >  1:2557199/1‑132 (MQ=255)
             tgATCGGCCTGTGGTGTGGCATGAAAGTGTTCAAGCTCGACCGCGATACCGCCTTGCTGACCGCCGCCGGCAGCGCCATCTGCGGCGCCGCCGCCGTGCTGGCATTCGAATCGGGGGGGGGCCC‑CGccccccccaa                                                                                                                  >  1:3915852/1‑132 (MQ=255)
             tgATCGGCCTGTGGTGTGGCATGAAAGTGTTCAAGCTCGACCGCGATACCGCCTTGCTGACCGCCGCCGGCAGCGCCATCTGCGGCGCCGCCGCCGTGCTGGCATTCGAATCGGGGGGGCGGCG‑CGccccccccca                                                                                                                  >  1:4330262/1‑132 (MQ=255)
             tgATCGGCCTGTGGTGTGGCATGAAAGTGTTCAAGCTCGACCGCGATACCGCCTTGCTGACCGCCGCCGGCAGCGCCATCTGCGGCGCCGCCGCCGTGCTGGCATTCGAATCGGGGGGGCGCGG‑cccccccccccc                                                                                                                  >  1:4446926/1‑132 (MQ=255)
             tgATCGGCCTGTGGTGTGGCATGAAAGTGTTCAAGCTCGACCGCGATACCGCCTTGCTGACCGCCGCCGGCAGCGCCATCTGCGGCGCCGCCGCCGTGCTGGCATTCGAATCGGGGCGGCGCCG‑CGcccccccaaa                                                                                                                  >  1:3280408/1‑132 (MQ=255)
             tgATCGGCCTGTGGTGTGGCATGAAAGTGTTCAAGCTCGACCGCGATACCGCCTTGCTGACCGCCGCCGGCAGCGCCATCTGCGGCGCCGCCGCCGTGCTGGCATTCGAATCGGGGCGGCGCAC‑ccccccccccaa                                                                                                                  >  1:1823031/1‑132 (MQ=255)
             tgATCGGCCTGTGGTGTGGCATGAAAGTGTTCAAGCTCGACCGCGATACCGCCTTGCTGACCGCCGCCGGCAGCGCCATCTGCGGCGCCGCCGCCGTGCTGGCATTCGAATCGGCGCTGCGCCG‑CGCCCCCCAGaa                                                                                                                  >  1:1796691/1‑136 (MQ=255)
             tgATCGGCCTGTGGTGTGGCATGAAAGTGTTCAAGCTCGACCGCGATACCGCCTTGCTGACCGCCGCCGGCAGCGCCATCTGCGGCGCCGCCGCCGTGCTGGCATTCGAATCGGCGCTGCGCCC‑CGcccccccaaa                                                                                                                  >  2:3523703/1‑132 (MQ=255)
             tgATCGGCCTGTGGTGTGGCATGAAAGTGTTCAAGCTCGACCGCGATACCGCCTTGCTGACCGCCGCCGGCAGCGCCATCTGCGGCGCCGCCGCCGTGCTGGCATTCGAATCGGCGCGGCCCCG‑cccccccccaaa                                                                                                                  >  1:3103387/1‑132 (MQ=255)
             tgATCGGCCTGTGGTGTGGCATGAAAGTGTTCAAGCTCGACCGCGATACCGCCTTGCTGACCGCCGCCGGCAGCGCCATCTGCGGCGCCGCCGCCGTGCTGGCATTCGAATAGGCGGAGCGCcc‑cccccccccaaa                                                                                                                  >  1:4685683/1‑132 (MQ=255)
             tgATCGGCCTGTGGTGTGGCATGAAAGTGTTCAAGCTCGACCGCGATACCGCCTTGCTGACCGCCGCCGGCAGCGCCATCTGCGGCGCCGCCGCCGTGCTGGCAATCGAATCGGCGCGGCGGAC‑CGcccccccaaa                                                                                                                  >  1:3060416/1‑132 (MQ=255)
             tgATCGGCCTGTGGTGTGGCATGAAAGTGTTCAAGCTCGACCGCGATACCGCCTTGCTGACCGCCGCCGGCAGCGCCATCTGCGGCGCCGCCGCCGAGCTGGCATTCCAATCGGCGCAGCGACG‑CGgcccccccaa                                                                                                                  >  2:3027755/1‑132 (MQ=255)
               aTCGGCCTGTGGTGTGGCATGAAAGTGTTCAAGCTCGACCGCGATACCGCCTTGCTGACCGCCGCCGGCCGCGCCATCTGCGGCGCCGCCGCCGTGCTGGCATTCGAATCGGCGGCGCCCCG‑cccccccccccaca                                                                                                                >  1:1922618/1‑130 (MQ=255)
               aTCGGCCTGTGGTGTGGCATGAAAGTGTTCAAGCTCGACCGCGATACCGCCTTGCTGACCGCCGCCGGCAGCGCCATCTGCGGCGCCGCCGCCGTGCTGGCATTCGAATCGGGGGGGGGCCG‑CGCCCCCCCCaaaa                                                                                                                >  2:3336178/1‑136 (MQ=255)
               aTCGGCCTGTGGTGTGGCATGAAAGTGTTCAAGCTCGACCGCGATACCGCCTTGCTGACCGCCGCCGGCAGCGCCATCTGCGGCGCCGCCGCCGTGCTGGCATTCGAATCGGGGGGGCGCGG‑CGgccccccccaca                                                                                                                >  1:3575165/1‑130 (MQ=255)
               aTCGGCCTGTGGTGTGGCATGAAAGTGTTCAAGCTCGACCGCGATACCGCCTTGCTGACCGCCGCCGGCAGCGCCATCTGCGGCGCCGCCGCCGTGCTGGCATTCGAATCGGGGGGGCGCAC‑CGCCCCCCCCaaaa                                                                                                                >  1:156609/1‑136 (MQ=255)
               aTCGGCCTGTGGTGTGGCATGAAAGTGTTCAAGCTCGACCGCGATACCGCCTTGCTGACCGCCGCCGGCAGCGCCATCTGCGGCGCCGCCGCCGTGCTGGCATTCGAATCGGGGCGGCGGCG‑CGCCCCCCCCaaaa                                                                                                                >  1:200441/1‑136 (MQ=255)
                  ggCCTGTGGTGTGGCATGAAAGTGTTCAAGCTCGACCGCGATACCGCCTTGCTGACCGCCGCCGGCAGCGCCATCTGCGGCGCCGCCGCCGTGCTGGCATTCGAATCGGGGGGGCGCCG‑CCCCCCCCCCaaaaaaa                                                                                                             >  1:382472/1‑133 (MQ=255)
                  ggCCTGTGGTGTGGCATGAAAGTGTTCAAGCTCGACCGCGATACCGCCTTGCTGACCGCCGCCGGCAGCGCCATCTGCGGCGCCGCCGCCGTGCTGGCATTCGAATCGGGGCAGCGCCG‑CGCCCCCCAGaaaaacc                                                                                                             >  2:1021567/1‑133 (MQ=255)
                  ggCCTGTGGTGTGGCATGAAAGTGTTCAAGCTCGACCGCGATACCGCCTTGCTGACCGCCGCCGGCAGCGCCATCTGCGGCGCCGCCGCCGTGCTGGCATTCGAATCGGCGCGGCGCAG‑CGCCCCCCaaaaaaccc                                                                                                             >  2:942789/1‑133 (MQ=255)
                  ggCCTGTGGTGTGGCATGAAAGTGTTCAAGCTCGACCGCGATACCGCCTTGCTGACCGCCGCCGGCAGCGCCATCTGCGGCGCCGCCGCCGTGCTGGCATTCGAATCGGCGCAGCGCAC‑CCCCCCCCCAaaaaacc                                                                                                             >  2:2599722/1‑133 (MQ=255)
                    ccTGAGGTGTGGCATGAAAGTGTTCAAGCTCGACCGCGATACCGCCTTGCTGACCGCCGCCGGCAGCGCCATCTGCCGCCCCGCCGCCCTGCTGGCATTCGAATCGGCGCGGCCACG‑CACCCCCCCCaaaaaacgc                                                                                                           >  2:528415/1‑131 (MQ=255)
                       gtggtgtgtCATTAATGTTTTCAAGCTCGCCGGCTAAACGGCGTTGCTGACCGCCGCGGGCAGCGCCTTCTGCGGCGCCGCCGCCGTGGTGGCTTTCGAATCGGGGCTGCGCTC‑CGCCCCCCATAAAAGCGCCATg                                                                                                        <  2:2314840/136‑1 (MQ=255)
                       gtggtgtggCATGAAAGTGTTCAAGCTCGACCGCGATACCGCCTTGCTGACCGCCGCCGGCAGCGCCATCTGCGGCGCCGCCGCCGTGCTGGCATTCGAATCGGCGCGGGGCCG‑CGCCCCCCAGAAAAGCGCCaag                                                                                                        >  2:4643363/1‑134 (MQ=255)
                       gtggtgtggCATGAAAGTGTTCAAGCTCGACCGCGATACCGCCTTGCTGACCGCCGCCGGCAGCGCCATCTGCGGCGCCGCCGCCGTGCTGGCATTCGAATCGGCGCAGCGCAG‑CGCCCCCCAAAAAAGCCCCagg                                                                                                        >  2:4437544/1‑134 (MQ=255)
                         ggtgtggCATGAAAGTGTTCAAGCTCGACCGCGATACCGCCTTGCTGACCGCCGCCGGCAGCGCCATCTGCGGCGCCGCCGCCGTGCTGGCATTCGAATCGGGGCGGCGCAG‑CCCCCCCCCAAAAAACCCCAAggg                                                                                                      >  2:870095/1‑135 (MQ=255)
                         ggtgtggCATGAAAGTGTTCAAGCTCGACCGCGATACCGCCTTGCTGACCGCCGCCGGCAGCGCCATCTGCGGCGCCGCCGCCGTGCTGGCATTCGAATCGGCGCGGCGCAG‑CGCCCCCCCAAAAAACGCCATGGc                                                                                                      >  2:4404687/1‑136 (MQ=255)
                         ggtgtggCATGAAAGTGTTCAAGCTCGACCGCGATACCGCCTTGCTGACCGCCGCCGGCAGCGCCATCTGCGGCGCCGCCGCCGGGCTGGCATTCGCATCGGCGCGGCGGCG‑CCGCCCCCACACAAACGcccaggc                                                                                                      >  2:2857641/1‑131 (MQ=255)
                            gtggCATTAAAGTGTTCAACCCCGACCGCGAACCCGCCTTCCTCCCCGCCGCCGGCCGCGCCCTCTGCGGCGCCGCCGCCGTGCGGGCATTCGATCCGGCGCTGCGCAG‑CGCCCCCCATAAAAGCGCCATGGCAGt                                                                                                   <  2:2235802/136‑1 (MQ=255)
                            gtggCATGATTGTGTGCACGCGCGCCCGCCATCCCGCGTGGCGCCCCGCCGCGGCCCGCGCCTGCTGCGGCGCCGCCGCGGGGCTGGCATTCGACGGGGCGCGGCGCAG‑CGCCCCCCATAAAAGCGCCATGGCAGt                                                                                                   <  2:164876/136‑1 (MQ=255)
                            gtggCATGAAAGTGTTCAAGCTCGACCGCGATACCGCCTTGCTGACCGCCGCCGGCAGCGCCATCTGCGGCGCCGCCGCCGTGCTGGCATTCGAATCGACGCTGCGCAG‑CGCCCCCCATAAAAGCGCCATGGCAGt                                                                                                   <  1:4678263/136‑1 (MQ=255)
                                cATGAAAGTGTTCAAGCTCGACCGCGATACCGCCTTGCTGACCGCCGCCGGCAGCGCCATCTGCGGCGCCGCCGCCGTGCTGGCATTCGAATCGGGGCGGGGCGC‑CCCCCCCCCAAAAAACGCCCAGGGAGTCggg                                                                                               >  1:4612958/1‑135 (MQ=255)
                                cATGAAAGTGTTCAAGCTCGACCGCGATACCGCCTTGCTGACCGCCGCCGGCAGCGCCATCTGCGGCGCCGCCGCCGTGCTGGCATTCGAATCGGCGCAGGCCAG‑CGCCCCCCAAAAAAACGCCCTAGCAgggcgc                                                                                               >  1:1954996/1‑131 (MQ=255)
                                cATGAAAGTGTTCAAGCTCGACCGCGATACCGCCTTGCTGACCGCCGCCGGCAGCGCCATCTGCGGCGCCGCCGCCGAGCTGGCATTCGAATCGGCGCAGCGCAC‑CCCCCCCCCAAAAAGCCCCAAAGCACTCGGa                                                                                               >  2:2306609/1‑136 (MQ=255)
                                cATGAAAGTGTTCAAGCTCGACAGCGATACCGCCTTGCTGACCGCCGCCGGCAGCGCCATCTGCGGCGCCGCCGCCGTGCTGGCATTCGAATCGGCGCTGCGTAG‑CGCCCCCCATAAAAGCGCCAAGGCAGtagca                                                                                               >  2:872071/1‑132 (MQ=255)
                                 attaaaGTGTCCACGCGCCCCCGCGATCCCGCTTTGCCCCCCGCCGCCGCCCGCGCCATCGGCGGCGCCCCCGCGGTGCTGCAATTCTAAGCGGCGGGGCGCCG‑CGCCCCCCATAAAAGCGCCATGGCAGTCGGaa                                                                                              <  2:1768025/133‑1 (MQ=255)
                                 aTGAAAGTGTTCAAGCTCGACCGCGATACCGCCTTGCTGACCGCCGCCGGCAGCGCCATCTGCGGCGCCGCCGCCGTGCTGGCATTCGAATCGGCGCTGCGCAG‑CGCCCCCCAAAAAAGCGCCATGGCAGTCGGaa                                                                                              >  2:3566035/1‑136 (MQ=255)
                                     aaGTGTTCAAGCTCGACCGCGATACCGCCTTGCTGACCGCCGCCGGCAGCGCCATCTGCGGCGCCGCCGCCGTGCTGGCATTCGAATCGGCGGGGCGCCGCCGCCCCCCA‑AAAAACGCCAAGGGAGTGGGGAggca                                                                                          >  2:293038/1‑133 (MQ=255)
                                     aaGTGTTCAAGCTCGACCGCGATACCGCCTTGCTGACCGCCGCCGGCAGCGCCATCTGCGGCGCCGCCGCCGTGCTGGCATTCGAATCGGCGCGGCGCCG‑CCCCCCCCCGAAAACCGCCAAGGCAGGCGGGAGCgg                                                                                          >  2:3468635/1‑135 (MQ=255)
                                      tgttttCACGCTCGACCGCTATACCGCTTGGCGGACCCGGCCCGGCAGCGCCTGCTGCGGCGCCGCCGCCGGGCTGTAATTCTAAGCGGCTCTGCGCTG‑CGCCCCCCATAAAAGCGCCATGGCAGTCGGAAGCGTa                                                                                         <  2:2720092/132‑1 (MQ=255)
                                      tgtgtTCAACCTCGCCGGCTATCCCGCCTTGCTGACCGCCGCCGCCTGCGCCTTGTGGGGCGCCGCGGCGGTGGTGGAATTCCAATCGGCGCTGCGCAG‑CGCCCCCCATAAAAGCGCCATGGCAGTCGGAAGCGTa                                                                                         <  2:3820433/135‑1 (MQ=255)
                                      tgtgcTCACGCGCGCCCGCGATCCCGCCTTGCCCCCCGCCGCCGGCCGCGCCCTCGGCGGCGCCGCCGGCGTGGTGTAATTCGACCCGGCGCGGCGCCC‑CCCCCCCCATAAAAGCGCCATGGCAGTCGGAAGCGTa                                                                                         <  2:3327530/135‑1 (MQ=255)
                                      aGTTTTCAAGCTCTCCCGCTAAACCGCCTTGGTCACCCCCGCCGGCAGCGCCTTCGGGGGCGCCGCCGCCGTGCTGGCATTCTAATGGGCGCTGCGCAG‑CGCCCCCCATAAAAGCGCCATGGCAGTCGGAAGCGTa                                                                                         <  2:3583257/136‑1 (MQ=255)
                                      aGTGTTCACGCGCGACCGCGATACCGCCTTGCGGACCGCCGCCGGCAGCGCCATCTGCGGCGCCGCCGCCGTGCTGGCATTCGAAGCGGCGGTGCGCCG‑CGCCCCCCATAAAAGCGCCATGGCAGTCGGAAGCGTa                                                                                         <  2:1720330/136‑1 (MQ=255)
                                           tCAAGCTCCCCCCCGATCCCGCTTTGCTGCCCCCCGCCGGCAGGGCCATGTGCGGCGCCGCCGCGGTGGTGTCATTCCAAGCGGCGCGGCGCCG‑CCCCCCCCATAAAAGCGCCATGGCAGTCGGAAGCGTAGTGCt                                                                                    <  2:2910334/136‑1 (MQ=255)
                                             aaGCTCGACCGCGATACCGCCTTGCTGACCGCCGCCGGCAGCGCCATCTGCGGCGCCGCCGCCGTGCTGGCATTCGACTCGGCGCGGCGCCC‑CGCCCCCCCAAAAAGCGCCCAGGCCGTCGGCAGGGTAGttgggg                                                                                  >  2:3969755/1‑131 (MQ=255)
                                             aaGCTCGACCGCGATACCGCCTTGCTGACCGCCGCCGGCAGCGCCATCTGCGGCGCCGCCGCCGTGCTGGCATTCGAATCGGGGGGGCGCCG‑CGCCCCCCCCAAAAACCCCCAGGGACGCGGAAGCGGAggggggt                                                                                  >  1:2268517/1‑130 (MQ=255)
                                             aaGCTCGACCGCGATACCGCCTTGCTGACCGCCGCCGGCAGCGCCATCTGCGGCGCCGCCGCCGTGCTGGCATTCGAATCGGGGCGGGGCCG‑CGCCCCCCCCAAAAAACCCCAAGCAGGCGGAAGCGGAgtggtgg                                                                                  >  1:959430/1‑135 (MQ=255)
                                             aaGCTCGACCGCGATACCGCCTTGCTGACCGCCGCCGGCAGCGCCATCTGCGGCGCCGCCGCCGTGCTGGCATTCGAATCGGGGCGGGGCAG‑CGCCCCCCCAAAAAACGCCAAGGGAGGCGGGAGGGGAGTGgtgt                                                                                  >  1:2703704/1‑136 (MQ=255)
                                             aaGCTCGACCGCGATACCGCCTTGCTGACCGCCGCCGGCAGCGCCATCTGCGGCGCCGCCGCCGTGCTGGCATTCGAATCGGCGCGGCGCCC‑CCCCCCCCCCAAAAACGCCCAGGCAGGCGGACGCCTAggtgggg                                                                                  >  1:1364019/1‑130 (MQ=255)
                                                  cGACCGCGATCGCGCCTGGCTGCCGGCCGCCGCCAGCCCCATCGGCGGCGCCGCCGCGGTGCTGGAACTCCACGGGGGCGTGCGCCG‑CGCCCCCCATAAAAGCGCCATGGCAGTCGGAAGCGTAGTGCTGTTCGGt                                                                             <  2:4207468/136‑1 (MQ=255)
                                                       gcgATACCGCCTTGCTGACCGCCGCCGGCAGCGCCATCTGCGGCGCCGCCGCCGTGCTGGCATTCGAATCGGGGCGGCGCCG‑CGCCCCCCAAAAAAAAGCCCAGGCGCGCGGGAGCGTAGTGGTGTTggggggcgt                                                                        >  2:3659428/1‑130 (MQ=255)
                                                       gcgATACCGCCTTGCTGACCGCCGCCGGCAGCGCCATCTGCGGCGCCGCCGCCGTGCTGGCATTCGAATCGGCGCGGCGCCG‑CGCCCCCCCAAAAAGCCCCCAGGCAATCGGAAGGGTATTGCTGGTCGGttactt                                                                        >  2:3094289/1‑131 (MQ=255)
                                                           tcccGCCTTGCTGCCCGCCCCCGGCAGCGCCATCGGCGGCGCCGCCGCCGTGCTGGCATTCTAAGCGGCGCTGCGCCG‑CGCCCCCCATAAAAGCGCCATGGCAGTCGGAAGCGTAGTGCTGTTCGGTACCTTGTCg                                                                    <  2:4579434/134‑1 (MQ=255)
                                                           tACCGCGTTGCTGACCGCCGCCGGCAGCGCCTTCTGCGGCGCCGCCGCGGTGCTGGATTTCGAATCGGCGCTGCGCAG‑CGCCCCCCATAAAAGCGCCATGGCAGTCGGAAGCGTAGTGCTGTTCGGTACCTTGTCg                                                                    <  2:1697542/136‑1 (MQ=255)
                                                           ccccgccgTGCCGCCCGCCGCCGGCCGCGCCCGCGGCGGCGCCGCCGCCGGTGTGCAATTCGACGCGGCGCGGCCCAC‑CCCCCCCCATAAAAGCGCCATGGCAGTCGGAAGCGTAGTGCTGTTCGGTACCTTGTCg                                                                    <  1:1712140/134‑1 (MQ=255)
                                                           ccccGCGTTGCTGCCCGCCGCGGGCAGCGCCATCTGGGGCGCCGCCGCGGTGCGGGCATTCGAAGCGGCGCTGCGCCG‑CGCCCCCCATAAAAGCGCCATGGCAGTCGGAAGCGTAGTGCTGTTCGGTACCTTGTCg                                                                    <  2:3766324/134‑1 (MQ=255)
                                                           acccGCCTTGCTGCCCGCCCCCGGCAGCGCCAGCTGGGGCGCCGCCGCCGTGCTGGCATTCTACTCGGCGCGGCGCAG‑CGCCCCCCATAAAAGCGCCATGGCAGTCGGAAGCGTAGTGCTGTTCGGTACCTTGTCg                                                                    <  2:1023357/134‑1 (MQ=255)
                                                                   tGCTGACCGCCACCGGCAGCGCCATCTGCGGCGCCGCCGCCGTGCTGGCATTCGAATCGGCGCTGTGCAG‑CGCCCCCCATAAAAGCGCCATGGCAGTCGGAAGCGTAGTGCTGTTCGGTACCTTGTCg                                                                    >  2:2699281/1‑128 (MQ=255)
                                                                      tGACCGCCGCCGGCAGCGCCATCTGCGGCGCCGCCGCCGTGCTGGCATTCGAATCGGCGCAGCGCCG‑CGCCCCCCCAAAAAGCCCCCAGGCAGTCGGAAGCGTAGTGGTGGTAGGGTCCGTGTCGATTTTACTTTa                                                         >  1:1838582/1‑136 (MQ=255)
                                                                          cgccgccgGCCGCGCCAGGGCCGGCGCGGCCGCCGGGCTTGCATAGGACGCGGCGCTCCCCCC‑CGCCCCCCATAAAAGCGCCATGGCAGTCGGAAGCGTAGTGCTGTTCGGTACCTTGTCGATGTTCCTTTACCCa                                                     <  1:2569172/136‑1 (MQ=255)
                                                                          cgccgccgGCAGCGCCTGCGGCGGCGCCGCCGCGGTGCTGGCATTCGAAGCGGCGCGGCGCCC‑CCCCCCCCATAAAAGCGCCATGGCAGTCGGAAGCGTAGTGCTGTTCGGTACCTTGTCGATGTTCCTTTACCCa                                                     <  1:4154252/136‑1 (MQ=255)
                                                                               ccgGCAGCGCCAGCGGCGGCGCCGCCGCCGTGCTGGATTTCGACGCGGCGCTGCGCCG‑CGCCCCCCATAAAAGCGCCATGGCAGTCGGAAGCGTAGTGCTGTTCGGTACCTTGTCGATGTTCCTTTACCCACTGGc                                                <  2:3743973/136‑1 (MQ=255)
                                                                                             gggggcgCCGCGGCGGTGCTGGCATTCGACGCGGCGCGTCGCCG‑CGCCCCCCATAAAAGCGCCATGGCAGTCGGAAGCGTAGTGCTGTTCGGTACCTTGTCGATGTTCCTTTACCCACTGGCGATCAATGCCggct                                  <  2:4004587/133‑1 (MQ=255)
                                                                                             gggggcgCCGCCGCGGGGCTGGAATGCTACGCGCCGCGGCGCCG‑CGCCCCCCATAAAAGCGCCATGGCAGTCGGAAGCGTAGTGCTGTTCGGTACCTTGTCGATGTTCCTTTACCCACTGGCGATCAATGCCggct                                  <  2:3982240/133‑1 (MQ=255)
                                                                                             ggcggggCCGCGGCGGGGGTGGCTTTCTATCCGGCGCTGCGCAG‑CGCCCCCCATAAAAGCGCCATGGCAGTCGGAAGCGTAGTGCTGTTCGGTACCTTGTCGATGTTCCTTTACCCACTGGCGATCAATGCCggct                                  <  2:2546666/135‑1 (MQ=255)
                                                                                             ggcggcgCCGCCGCCGTGCTGGCTTTCGAGGGGGCGCGGCGCAG‑CCCCCCCCATAAAAGCGCCATGGCAGTCGGAAGCGTAGTGCTGTTCGGTACCTTGTCGATGTTCCTTTACCCACTGGCGATCAATGCCggct                                  <  1:4626034/135‑1 (MQ=255)
                                                                                             ggcggcgCCGCCGCCGTGCTGGCTTCCCCCGCGGCGCGGCCCCC‑CCCCCCCCATAAAAGCGCCATGGCAGTCGGAAGCGTAGTGCTGTTCGGTACCTTGTCGATGTTCCTTTACCCACTGGCGATCAATGCCggct                                  <  1:1236444/135‑1 (MQ=255)
                                                                                               gggcgCCGCGGCGGTGGTGGCTTTCGAAGGGGCGCGGCGCCG‑CGCCCCCCATAAAAGCGCCATGGCAGTCGGAAGCGTAGTGCTGTTCGGTACCTTGTCGATGTTCCTTTACCCACTGGCGATCAATGCCggctgg                                <  1:1746202/135‑1 (MQ=255)
                                                                                               gggcgCCGCCGGGGTGCTTGCCTTCGCCGCGGCCCGGCGCCC‑CCCCCCCCATAAAAGCGCCATGGCAGTCGGAAGCGTAGTGCTGTTCGGTACCTTGTCGATGTTCCTTTACCCACTGGCGATCAATGCCggctgg                                <  1:1020361/135‑1 (MQ=255)
                                                                                               gggcgCCGCCGCCGTGCTGGCATTCTTATCGGCGCGGCGGAG‑CGCCCCCCATAAAAGCGCCATGGCAGTCGGAAGCGTAGTGCTGTTCGGTACCTTGTCGATGTTCCTTTACCCACTGGCGATCAATGCCggctgg                                <  2:3060416/135‑1 (MQ=255)
                                                                                               gggcgCCGCCGCCGGGGTGGGATTCGAAGCGGCGCGGCGCAC‑CGCCCCCCATAAAAGCGCCATGGCAGTCGGAAGCGTAGTGCTGTTCGGTACCTTGTCGATGTTCCTTTACCCACTGGCGATCAATGCCggctgg                                <  2:3854353/135‑1 (MQ=255)
                                                                                                gccgacgccgcggGGCTGGAATTCTATGCGGCTCTGGTCCG‑CGCCCCCCATAAAAGCGCCATGGCAGTCGGAATCGTAGTGCTGTTCGGTACCTTGTCGATGTTCCTTTACACACTGGCGATCAATGCCggctggc                               <  2:2253383/131‑1 (MQ=255)
                                                                                                  cgccgccgcCGTGCTGTCTTCCGACCCGGCCCGGCGCCG‑CCCCCCCCATAAAAGCGCCATGGCAGTCGGAAGCGTAGTGCTGTTCGGTACCTTGTCGATGTTCCTTTACCCACTGGCGATCAATGCCGgctggctg                             <  2:4007259/136‑1 (MQ=255)
                                                                                                   gccgccgccGGGCTGGAATTCTATCGGGCGCGGCGCAG‑CGCCCCCCATAAAAGCGCCATGGCAGTCGGAAGCGTAGTGCTGTTCGGTACCTTGTCGATGTTCCTTTACCCACTGGCGATCAATGCCGGCTGGCTGc                            <  2:382472/136‑1 (MQ=255)
                                                                                                     cgccgccgTGCTGCTATTCGCCGCGGCGCGGCGCCC‑CCCCCCCCATAAAAGCGCCATGGCAGTCGGAAGCGTAGTGCTGTTCGGTACCTTGTCGATGTTCCTTTACCCACTGGCGATCAATGCCGGCTGGCTGCAt                          <  1:3855071/136‑1 (MQ=255)
                                                                                                       ccgccgTGCTGGCATTCTAATCGGCGCTGCGCCG‑CGCCCCCCATAAAAGCGCCATGGCAGTCGGAAGCGTAGTGCTGTTCGGTACCTTGTCGATGTTCCTTTACCCACTGGCGATCAATGCCGGCTGGCTGCATCt                        <  2:493199/136‑1 (MQ=255)
                                                                                                        ggccgGGGTGGCTTTCGATGCGGCGCTGCGCCG‑CGCCCCCCATAAAAGCGCCATGGCAGTCGGAAGCGTAGTGCTGTTCGGTACCTTGTCGATGTTCCTTTACCCACTGGCGATCAATGCCGGCTGGCTGCATCTg                       <  2:2400070/135‑1 (MQ=255)
                                                                                                                        gAATCGGCGCTGCGCAG‑CGCCCCCCATAAAAGCGCCATGGCAGTCGGAAGCGTAGTGCTGTTCGGTACCTTGTCGATGTTCCTTTACCCACTGGCGATCAATGCCGGCTGGCTGCATCTGGATACCATGGGCGCCg       <  2:2773664/136‑1 (MQ=255)
                                                                                                                        caagcggcgcggcgCCG‑CGCCCCCCATAAAAGCGCCATGGCAGTCGGAAGCGTAGTGCTGTTCGGTACCTTGTCGATGTTCCTTTACCCACTGGCGATCAATGCCGGCTGGCTGCATCTGGATACCATGGGCGCCg       <  2:286844/132‑1 (MQ=255)
                                                                                                                          aTCGGCGCTGCGCAG‑CGCCCCCCAAAAAATCGCCATGGCAGTCGGAAGCGTAGTGCTGTTCGGTTCCATGTAGATGTTACTTTAAACACTGGCGGTAAAATCCGGCTGGCTGCATCAGGGTACCAAGGGCGCCggg     >  2:3517485/1‑136 (MQ=255)
                                                                                                                             ggcgcggcgcTG‑CGCCCCCCATAAAAGCGCCATGGCAGTCGGAAGCGTAGTGCTGTTCGGTACCTTGTCGATGTTCCTTTACCCACTGGCGATCAATGCCGGCTGGCTGCATCTGGATACCATGGGCGCCGGgctg  <  2:1800458/136‑1 (MQ=255)
                                                                                                                             ggcgcggcgcCG‑CGCCCCCCATAAAAGCGCCATGGCAGTCGGAAGCGTAGTGCTGTTCGGTACCTTGTCGATGTTCCTTTACCCACTGGCGATCAATGCCGGCTGGCTGCATCTGGATACCATGGGCGCCGGgctg  <  2:4239127/136‑1 (MQ=255)
                                                                                                                             ggcgcggcgcAG‑CGCCCCCCATAAAAGCGCCATGGCAGTCGGAAGCGTAGTGCTGTTCGGTACCTTGTCGATGTTCCTTTACCCACTGGCGATCAATGCCGGCTGGCTGCATCTGGATACCATGGGCGCCGGgctg  <  2:801557/136‑1 (MQ=255)
                                                                                                                             ggccgtGCGCTG‑CCACCCCCATAAAAGCGCCATGGCAGTCGGAAGCGTAGTGCTGTTCGGTACCTTGTCGATGTTCCTTTACCCACTGGCGATCAATGCCGGCTGGCTGCATCTGGATACCATGGGCGCCGGgctg  <  1:838181/131‑1 (MQ=255)
                                                                                                                             ggCGCTCCGGCG‑CCCCCCCCATAACAGCGCCATGGCAGTCGGAAGCGTAGTGCTGTTCGGTACCTTGTCGATGTTCCTTTACCCACTGGCGATCAATGCCGGCTGGCTGCATCTGGATACCATGGGCGCCGGgctg  <  1:248880/136‑1 (MQ=255)
                                                                                                                                  |                                                                                                                                   
ACCAGTACCCTGCTGATCGGCCTGTGGTGTGGCATGAAAGTGTTCAAGCTCGACCGCGATACCGCCTTGCTGACCGCCGCCGGCAGCGCCATCTGCGGCGCCGCCGCCGTGCTGGCATTCGAATCGGCGCTGCGCAG‑CGCCCCCCATAAAAGCGCCATGGCAGTCGGAAGCGTAGTGCTGTTCGGTACCTTGTCGATGTTCCTTTACCCACTGGCGATCAATGCCGGCTGGCTGCATCTGGATACCATGGGCGCCGGGCTG  >  NC_002947/4160302‑4160562

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑